Pannonibacter phragmitetus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Stappiaceae; Pannonibacter

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4943 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U3NZC0|A0A0U3NZC0_9RHOB Malate dehydrogenase OS=Pannonibacter phragmitetus OX=121719 GN=mdh PE=3 SV=1
MM1 pKa = 8.04SLMGSLNSAISALAAQSQALSNISSNLANSSTTAYY36 pKa = 9.23KK37 pKa = 10.61ASSTSFSNLIAGSSTSTSGGVTASTRR63 pKa = 11.84ASVSDD68 pKa = 3.47QGLLTATGNSTNLAIDD84 pKa = 3.89GSGFFVVSSSVTGGQTYY101 pKa = 6.99YY102 pKa = 10.29TRR104 pKa = 11.84NGEE107 pKa = 4.36FEE109 pKa = 4.07VDD111 pKa = 3.32QNGYY115 pKa = 9.76LVNNGYY121 pKa = 10.23YY122 pKa = 10.75LMGWQTDD129 pKa = 3.03ADD131 pKa = 4.11GNIIGGTSEE140 pKa = 4.33ASLVPIDD147 pKa = 3.88IDD149 pKa = 4.51AIQSSVGATTAVGIQANLPADD170 pKa = 3.66AVEE173 pKa = 4.96GDD175 pKa = 3.91TFSTTVEE182 pKa = 4.26IYY184 pKa = 10.62DD185 pKa = 4.29SLGSTHH191 pKa = 6.17NMSVTWTKK199 pKa = 10.18TGEE202 pKa = 4.41NTWEE206 pKa = 4.07MTFDD210 pKa = 4.56DD211 pKa = 4.26PTLNGTTSGTVSTDD225 pKa = 3.33AIEE228 pKa = 3.93ITFNSDD234 pKa = 2.36GTLASTNPSPATLSVTGWTTGAADD258 pKa = 3.83TEE260 pKa = 4.58VTLDD264 pKa = 3.89LGTSGLANGLTQYY277 pKa = 11.52ASGSSDD283 pKa = 3.63PYY285 pKa = 11.31LKK287 pKa = 10.39LDD289 pKa = 4.53KK290 pKa = 9.78ITNDD294 pKa = 3.19GLPYY298 pKa = 10.69GSLTGVSITSDD309 pKa = 3.02GSVIASYY316 pKa = 11.63DD317 pKa = 3.41NGDD320 pKa = 3.0EE321 pKa = 3.93RR322 pKa = 11.84TIYY325 pKa = 9.79KK326 pKa = 10.13IPVATFTNSNGLTLNSDD343 pKa = 3.43GMYY346 pKa = 10.31SRR348 pKa = 11.84SPTSGNSTLQIAGTSGAGSINGGYY372 pKa = 10.27LEE374 pKa = 5.69ASTTDD379 pKa = 2.86TSTEE383 pKa = 3.89FSRR386 pKa = 11.84MLTAQQAYY394 pKa = 9.57SAASQIISTSNSMFDD409 pKa = 3.43SLLQAVRR416 pKa = 3.68

Molecular weight: 42.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L0J4M4|A0A0L0J4M4_9RHOB Disulfide bond formation protein DsbA OS=Pannonibacter phragmitetus OX=121719 GN=APZ00_18445 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.21RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.48GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.24NGRR28 pKa = 11.84LVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.19GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4943

0

4943

1591901

32

3054

322.1

34.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.746 ± 0.048

0.827 ± 0.012

5.41 ± 0.024

5.844 ± 0.03

3.754 ± 0.026

8.767 ± 0.034

1.97 ± 0.019

5.148 ± 0.028

3.141 ± 0.023

10.546 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.605 ± 0.017

2.493 ± 0.019

5.109 ± 0.031

3.246 ± 0.024

6.842 ± 0.036

5.594 ± 0.027

5.35 ± 0.029

7.194 ± 0.031

1.272 ± 0.016

2.141 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski