Sorangiineae bacterium NIC37A_2
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4071 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W9QGE6|A0A1W9QGE6_9DELT Fer2_BFD domain-containing protein OS=Sorangiineae bacterium NIC37A_2 OX=1970199 GN=B6A08_03530 PE=4 SV=1
MM1 pKa = 7.49 GGALSFGGGDD11 pKa = 3.48 EE12 pKa = 4.38 VVLIDD17 pKa = 6.31 DD18 pKa = 5.1 FDD20 pKa = 7.2 DD21 pKa = 5.2 DD22 pKa = 4.83 DD23 pKa = 6.69 ALFEE27 pKa = 5.1 SNGLNGGWYY36 pKa = 10.05 AYY38 pKa = 10.67 GDD40 pKa = 3.99 GSNGTLTHH48 pKa = 6.38 SEE50 pKa = 4.02 GLVPVSGGVRR60 pKa = 11.84 GGALRR65 pKa = 11.84 VQSEE69 pKa = 4.73 GFTAWGSVFGAYY81 pKa = 10.11 LSEE84 pKa = 4.25 GCLYY88 pKa = 10.85 DD89 pKa = 4.78 GSAFDD94 pKa = 5.3 GLTFYY99 pKa = 11.43 AKK101 pKa = 10.29 GQVEE105 pKa = 4.46 GGPNQEE111 pKa = 3.71 LSVRR115 pKa = 11.84 LVSLEE120 pKa = 4.27 DD121 pKa = 3.56 MPPEE125 pKa = 4.12 QGGTCDD131 pKa = 3.49 SDD133 pKa = 3.27 CWNSHH138 pKa = 4.85 RR139 pKa = 11.84 VNIEE143 pKa = 3.41 VGGCWRR149 pKa = 11.84 RR150 pKa = 11.84 YY151 pKa = 7.33 SFRR154 pKa = 11.84 FDD156 pKa = 2.85 EE157 pKa = 4.77 FLRR160 pKa = 11.84 AGQSTSSVVPSEE172 pKa = 3.77 LHH174 pKa = 6.89 LIDD177 pKa = 4.51 FALSTVDD184 pKa = 5.49 SADD187 pKa = 2.95 VWLDD191 pKa = 3.31 EE192 pKa = 4.21 LAFFIGDD199 pKa = 3.42 APGEE203 pKa = 4.09 EE204 pKa = 4.83 EE205 pKa = 4.51 ICDD208 pKa = 3.53
Molecular weight: 22.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.795
IPC_protein 3.783
Toseland 3.579
ProMoST 3.91
Dawson 3.77
Bjellqvist 3.948
Wikipedia 3.694
Rodwell 3.617
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.101
Thurlkill 3.617
EMBOSS 3.706
Sillero 3.91
Patrickios 1.1
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.814
Protein with the highest isoelectric point:
>tr|A0A1W9QCZ8|A0A1W9QCZ8_9DELT Uncharacterized protein OS=Sorangiineae bacterium NIC37A_2 OX=1970199 GN=B6A08_10125 PE=4 SV=1
MM1 pKa = 7.62 AIWARR6 pKa = 11.84 MARR9 pKa = 11.84 AWAVRR14 pKa = 11.84 PWTLRR19 pKa = 11.84 AGMSVRR25 pKa = 11.84 VGVRR29 pKa = 11.84 SVLAALFQAPVVPRR43 pKa = 11.84 QGEE46 pKa = 4.3 GMPLAGARR54 pKa = 11.84 LGAPRR59 pKa = 11.84 QAAPQAVATLLVVLRR74 pKa = 11.84 AQGARR79 pKa = 11.84 IRR81 pKa = 11.84 GARR84 pKa = 11.84 AQARR88 pKa = 11.84 VARR91 pKa = 11.84 TPVGPGGG98 pKa = 3.43
Molecular weight: 10.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.472
IPC2_protein 11.038
IPC_protein 12.647
Toseland 12.808
ProMoST 13.32
Dawson 12.808
Bjellqvist 12.808
Wikipedia 13.29
Rodwell 12.31
Grimsley 12.852
Solomon 13.32
Lehninger 13.217
Nozaki 12.808
DTASelect 12.808
Thurlkill 12.808
EMBOSS 13.32
Sillero 12.808
Patrickios 12.047
IPC_peptide 13.32
IPC2_peptide 12.31
IPC2.peptide.svr19 9.169
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4071
0
4071
1466748
33
3805
360.3
39.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.182 ± 0.043
1.059 ± 0.024
5.268 ± 0.032
7.175 ± 0.047
3.62 ± 0.024
8.834 ± 0.061
1.896 ± 0.015
4.043 ± 0.029
3.359 ± 0.032
10.786 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.693 ± 0.016
2.172 ± 0.023
5.928 ± 0.037
2.936 ± 0.018
7.699 ± 0.047
6.714 ± 0.034
5.061 ± 0.024
7.285 ± 0.034
1.146 ± 0.014
2.139 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here