Oceanotoga teriensis
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2638 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A316D2T6|A0A316D2T6_9BACT Putative MATE family efflux protein OS=Oceanotoga teriensis OX=515440 GN=C7380_10770 PE=4 SV=1
MM1 pKa = 7.58 SKK3 pKa = 10.72 RR4 pKa = 11.84 LTVLLFIIFTFILSFGSSYY23 pKa = 11.06 TMNSFDD29 pKa = 3.81 SSEE32 pKa = 4.09 LNRR35 pKa = 11.84 INSAMSTDD43 pKa = 3.41 KK44 pKa = 11.57 GNFSLNVAKK53 pKa = 10.62 VIYY56 pKa = 10.21 DD57 pKa = 3.63 FYY59 pKa = 11.5 NFDD62 pKa = 3.61 WAGNEE67 pKa = 3.7 YY68 pKa = 10.8 LLRR71 pKa = 11.84 DD72 pKa = 4.24 LEE74 pKa = 5.72 DD75 pKa = 3.16 IMNLYY80 pKa = 10.62 SEE82 pKa = 5.93 DD83 pKa = 3.73 IYY85 pKa = 11.66 SEE87 pKa = 4.9 DD88 pKa = 3.93 FDD90 pKa = 6.24 SEE92 pKa = 4.51 FDD94 pKa = 4.46 DD95 pKa = 4.29 EE96 pKa = 6.17 DD97 pKa = 4.02 YY98 pKa = 11.52 LEE100 pKa = 4.27 KK101 pKa = 11.01 LEE103 pKa = 5.12 EE104 pKa = 4.14 FFKK107 pKa = 10.97 SLKK110 pKa = 10.8 SSDD113 pKa = 4.12 VYY115 pKa = 11.84 DD116 pKa = 4.07 LMVSIKK122 pKa = 10.9 DD123 pKa = 3.55 EE124 pKa = 4.86 LKK126 pKa = 11.18 DD127 pKa = 3.52 LDD129 pKa = 4.26 EE130 pKa = 5.22 NKK132 pKa = 10.6 DD133 pKa = 3.64 SLVFLSKK140 pKa = 10.31 EE141 pKa = 4.01 AYY143 pKa = 9.94 DD144 pKa = 4.76 DD145 pKa = 3.78 FVKK148 pKa = 10.89 FINVDD153 pKa = 3.08 SDD155 pKa = 3.97 PYY157 pKa = 10.58 FVEE160 pKa = 4.5 FDD162 pKa = 3.84 YY163 pKa = 10.1 EE164 pKa = 4.63 TVGNFAFDD172 pKa = 3.56 YY173 pKa = 7.9 RR174 pKa = 11.84 TLKK177 pKa = 10.66 ILDD180 pKa = 3.32 ILANTFVYY188 pKa = 10.5 MNDD191 pKa = 3.45 NPSMLEE197 pKa = 3.83 YY198 pKa = 10.15 YY199 pKa = 10.76 HH200 pKa = 6.74 NFFSYY205 pKa = 10.76 FEE207 pKa = 4.44 SDD209 pKa = 3.36 PDD211 pKa = 3.71 EE212 pKa = 4.62 EE213 pKa = 4.73 DD214 pKa = 4.48 LIRR217 pKa = 11.84 PEE219 pKa = 4.2 ANSVFSQMEE228 pKa = 4.07 YY229 pKa = 10.9 VEE231 pKa = 5.02 PEE233 pKa = 4.04 DD234 pKa = 5.65 FEE236 pKa = 4.92 PLLKK240 pKa = 10.6 KK241 pKa = 10.48 LAAVDD246 pKa = 5.07 GDD248 pKa = 4.45 SEE250 pKa = 4.64 WISLRR255 pKa = 11.84 DD256 pKa = 3.33 IFIPEE261 pKa = 4.06 ILILPPSYY269 pKa = 10.92 EE270 pKa = 3.98 YY271 pKa = 9.84 FTISDD276 pKa = 3.59 YY277 pKa = 11.96 AMRR280 pKa = 11.84 GMLLIDD286 pKa = 4.62 DD287 pKa = 5.45 FEE289 pKa = 4.77 TSDD292 pKa = 3.58 LFVNKK297 pKa = 9.78 RR298 pKa = 11.84 YY299 pKa = 10.37 ADD301 pKa = 3.62 LVDD304 pKa = 3.91 YY305 pKa = 10.3 FMNTSEE311 pKa = 5.21 ADD313 pKa = 3.51 DD314 pKa = 3.77 FVYY317 pKa = 10.79 YY318 pKa = 9.41 YY319 pKa = 11.04 YY320 pKa = 9.94 PDD322 pKa = 3.29 SYY324 pKa = 11.24 GIKK327 pKa = 10.05 EE328 pKa = 4.61 DD329 pKa = 3.39 FDD331 pKa = 4.25 KK332 pKa = 11.34 VLKK335 pKa = 10.32 EE336 pKa = 4.13 NYY338 pKa = 9.02 NFRR341 pKa = 11.84 YY342 pKa = 10.14 ANPILGFLNVSFDD355 pKa = 3.77 EE356 pKa = 4.73 FLMEE360 pKa = 5.33 EE361 pKa = 4.19 DD362 pKa = 4.52 LEE364 pKa = 5.43 DD365 pKa = 3.53 IVSEE369 pKa = 4.2 FWMSINGVKK378 pKa = 10.26 VYY380 pKa = 10.74 NFRR383 pKa = 11.84 DD384 pKa = 3.96 FVEE387 pKa = 4.52 EE388 pKa = 3.87 FLNSYY393 pKa = 9.93 KK394 pKa = 10.98 DD395 pKa = 3.44 VMNFKK400 pKa = 10.52 FRR402 pKa = 11.84 MMFNIDD408 pKa = 3.12 MSTGYY413 pKa = 9.88 EE414 pKa = 4.0 EE415 pKa = 4.54 PGEE418 pKa = 4.16 VVEE421 pKa = 4.6 PLVLGLDD428 pKa = 3.52 MNMTFNYY435 pKa = 8.81 PAYY438 pKa = 9.51 KK439 pKa = 10.64 DD440 pKa = 3.83 PFDD443 pKa = 3.82 VGAMNLRR450 pKa = 11.84 FKK452 pKa = 11.11 PEE454 pKa = 3.41 IFNVVMSEE462 pKa = 4.04 FAKK465 pKa = 10.87 LEE467 pKa = 3.67 QGYY470 pKa = 9.88 FNISEE475 pKa = 4.13 IVSNISEE482 pKa = 4.19 YY483 pKa = 10.78 TDD485 pKa = 3.61 FLKK488 pKa = 11.09 VEE490 pKa = 4.14 NNDD493 pKa = 3.22 LVFYY497 pKa = 10.73 LNVDD501 pKa = 3.48 NYY503 pKa = 10.17 FDD505 pKa = 4.48 SEE507 pKa = 4.11 ISFVNFMSDD516 pKa = 4.36 DD517 pKa = 3.58 IVNLLSMFLMTAMSSDD533 pKa = 3.55 YY534 pKa = 11.31 NYY536 pKa = 11.59
Molecular weight: 63.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.795
IPC_protein 3.821
Toseland 3.605
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.643
Grimsley 3.503
Solomon 3.795
Lehninger 3.745
Nozaki 3.897
DTASelect 4.126
Thurlkill 3.643
EMBOSS 3.719
Sillero 3.935
Patrickios 1.265
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.815
Protein with the highest isoelectric point:
>tr|A0A316D0Q2|A0A316D0Q2_9BACT 3D (Asp-Asp-Asp) domain-containing protein OS=Oceanotoga teriensis OX=515440 GN=C7380_10290 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 9.91 QPSRR9 pKa = 11.84 IKK11 pKa = 10.52 RR12 pKa = 11.84 KK13 pKa = 7.89 RR14 pKa = 11.84 THH16 pKa = 6.05 GFLVRR21 pKa = 11.84 KK22 pKa = 7.06 RR23 pKa = 11.84 TKK25 pKa = 10.3 AGSSVLRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.65 GRR39 pKa = 11.84 KK40 pKa = 8.66 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.45
IPC2_protein 11.169
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.427
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.149
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.074
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2638
0
2638
858968
26
1762
325.6
37.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.394 ± 0.047
0.641 ± 0.014
5.608 ± 0.037
7.409 ± 0.051
5.524 ± 0.045
5.579 ± 0.048
1.243 ± 0.018
11.443 ± 0.062
10.147 ± 0.062
9.038 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.532 ± 0.021
7.348 ± 0.056
2.805 ± 0.024
2.15 ± 0.025
2.768 ± 0.027
6.512 ± 0.038
4.439 ± 0.032
5.044 ± 0.042
0.754 ± 0.015
4.622 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here