Aspergillus awamori (Black koji mold)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11139 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A401KDG7|A0A401KDG7_ASPAW RNA polymerase I-specific transcription initiation factor rrn5 OS=Aspergillus awamori OX=105351 GN=AAWM_00202 PE=4 SV=1
MM1 pKa = 7.07KK2 pKa = 9.9TYY4 pKa = 10.84GLWSTLAALGSFTLSSAKK22 pKa = 9.68TINFVAHH29 pKa = 7.08EE30 pKa = 4.68DD31 pKa = 3.7DD32 pKa = 5.65DD33 pKa = 5.68LLFLSPDD40 pKa = 4.47LIRR43 pKa = 11.84DD44 pKa = 3.6ILSGEE49 pKa = 4.39PIRR52 pKa = 11.84TVFLTAGDD60 pKa = 4.0AGLGSDD66 pKa = 3.5YY67 pKa = 11.0WNARR71 pKa = 11.84EE72 pKa = 4.0EE73 pKa = 4.44GSRR76 pKa = 11.84AAYY79 pKa = 10.58ARR81 pKa = 11.84MAVASNNWDD90 pKa = 3.2EE91 pKa = 5.09DD92 pKa = 4.52VIFVGDD98 pKa = 4.0KK99 pKa = 10.94SITLYY104 pKa = 10.31TLQDD108 pKa = 4.01DD109 pKa = 4.91DD110 pKa = 5.76DD111 pKa = 5.1ISLAFMHH118 pKa = 6.84LPDD121 pKa = 4.93GNMDD125 pKa = 3.49GTGFGPDD132 pKa = 3.85QNDD135 pKa = 3.5SLQKK139 pKa = 10.46LWNGDD144 pKa = 2.49IDD146 pKa = 4.34VIRR149 pKa = 11.84TIDD152 pKa = 3.45GSGIEE157 pKa = 4.11YY158 pKa = 9.48TRR160 pKa = 11.84DD161 pKa = 3.19EE162 pKa = 4.5LLEE165 pKa = 4.35TIAALIDD172 pKa = 4.57DD173 pKa = 5.25FDD175 pKa = 5.85PDD177 pKa = 3.61EE178 pKa = 4.89VKK180 pKa = 10.28TGDD183 pKa = 3.6YY184 pKa = 11.43VNDD187 pKa = 4.35FGDD190 pKa = 4.38GDD192 pKa = 3.99HH193 pKa = 7.18SDD195 pKa = 4.75HH196 pKa = 6.31YY197 pKa = 10.96ACGYY201 pKa = 10.12FVDD204 pKa = 4.93NALQQADD211 pKa = 3.89SDD213 pKa = 4.19AGLTGYY219 pKa = 10.12YY220 pKa = 9.95GYY222 pKa = 9.31PIQYY226 pKa = 9.27MDD228 pKa = 4.34VNLDD232 pKa = 3.65DD233 pKa = 6.06SDD235 pKa = 5.69IEE237 pKa = 4.29DD238 pKa = 3.65KK239 pKa = 11.42TNIFYY244 pKa = 10.62EE245 pKa = 4.21YY246 pKa = 10.82ADD248 pKa = 4.29YY249 pKa = 8.64DD250 pKa = 4.13TATCASDD257 pKa = 4.01DD258 pKa = 3.99ACSSRR263 pKa = 11.84PEE265 pKa = 4.3SAWLLRR271 pKa = 11.84EE272 pKa = 4.08YY273 pKa = 10.62EE274 pKa = 4.27VV275 pKa = 3.97

Molecular weight: 30.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A401LA08|A0A401LA08_ASPAW Uncharacterized protein (Fragment) OS=Aspergillus awamori OX=105351 GN=AAWM_11219 PE=4 SV=1
MM1 pKa = 7.02PQSAAIHH8 pKa = 5.48GHH10 pKa = 6.37PGAPLAASLPRR21 pKa = 11.84HH22 pKa = 6.04PRR24 pKa = 11.84ASHH27 pKa = 5.9PPSRR31 pKa = 11.84PVSPRR36 pKa = 11.84GASHH40 pKa = 6.78HH41 pKa = 6.82PISPGQLVNSLRR53 pKa = 11.84QHH55 pKa = 6.6ARR57 pKa = 11.84TQLSAGTGRR66 pKa = 11.84LPAWPPAVSAGRR78 pKa = 11.84SAAPALQVLRR88 pKa = 11.84RR89 pKa = 11.84SVAAILRR96 pKa = 11.84ANVLWSEE103 pKa = 4.13SDD105 pKa = 2.89SWGRR109 pKa = 11.84PPAHH113 pKa = 6.49RR114 pKa = 11.84VLATVSRR121 pKa = 11.84GQLVNGPLSRR131 pKa = 11.84TRR133 pKa = 11.84ARR135 pKa = 11.84TASLPP140 pKa = 3.59

Molecular weight: 14.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11139

0

11139

6005040

66

7054

539.1

59.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.511 ± 0.02

1.28 ± 0.008

5.628 ± 0.015

6.123 ± 0.023

3.71 ± 0.014

6.776 ± 0.018

2.463 ± 0.009

4.99 ± 0.015

4.384 ± 0.019

9.184 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.168 ± 0.008

3.587 ± 0.011

6.034 ± 0.025

4.057 ± 0.016

6.092 ± 0.015

8.27 ± 0.024

6.047 ± 0.017

6.262 ± 0.014

1.512 ± 0.009

2.923 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski