Amino acid dipepetide frequency for Aspergillus awamori (Black koji mold)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.909AlaAla: 8.909 ± 0.056	1.125AlaCys: 1.125 ± 0.015	4.232AlaAsp: 4.232 ± 0.027	5.034AlaGlu: 5.034 ± 0.035	3.167AlaPhe: 3.167 ± 0.024	5.734AlaGly: 5.734 ± 0.04	1.816AlaHis: 1.816 ± 0.02	4.365AlaIle: 4.365 ± 0.027	3.657AlaLys: 3.657 ± 0.033	7.835AlaLeu: 7.835 ± 0.041	1.997AlaMet: 1.997 ± 0.018	2.889AlaAsn: 2.889 ± 0.024	4.615AlaPro: 4.615 ± 0.042	3.337AlaGln: 3.337 ± 0.032	4.873AlaArg: 4.873 ± 0.033	7.231AlaSer: 7.231 ± 0.043	5.313AlaThr: 5.313 ± 0.034	5.508AlaVal: 5.508 ± 0.036	1.203AlaTrp: 1.203 ± 0.015	2.268AlaTyr: 2.268 ± 0.02	0.0AlaXaa: 0.0 ± 0.0
Cys	0.973CysAla: 0.973 ± 0.015	0.236CysCys: 0.236 ± 0.007	0.685CysAsp: 0.685 ± 0.012	0.614CysGlu: 0.614 ± 0.011	0.561CysPhe: 0.561 ± 0.009	0.929CysGly: 0.929 ± 0.014	0.364CysHis: 0.364 ± 0.009	0.74CysIle: 0.74 ± 0.013	0.468CysLys: 0.468 ± 0.01	1.402CysLeu: 1.402 ± 0.017	0.281CysMet: 0.281 ± 0.007	0.43CysAsn: 0.43 ± 0.009	0.671CysPro: 0.671 ± 0.011	0.491CysGln: 0.491 ± 0.01	0.808CysArg: 0.808 ± 0.011	0.973CysSer: 0.973 ± 0.013	0.722CysThr: 0.722 ± 0.013	0.856CysVal: 0.856 ± 0.012	0.226CysTrp: 0.226 ± 0.007	0.37CysTyr: 0.37 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	4.594AspAla: 4.594 ± 0.03	0.643AspCys: 0.643 ± 0.012	3.959AspAsp: 3.959 ± 0.034	4.231AspGlu: 4.231 ± 0.032	2.113AspPhe: 2.113 ± 0.02	3.923AspGly: 3.923 ± 0.035	1.293AspHis: 1.293 ± 0.016	3.169AspIle: 3.169 ± 0.025	2.195AspLys: 2.195 ± 0.022	5.181AspLeu: 5.181 ± 0.034	1.26AspMet: 1.26 ± 0.014	1.906AspAsn: 1.906 ± 0.017	3.36AspPro: 3.36 ± 0.024	1.92AspGln: 1.92 ± 0.02	3.121AspArg: 3.121 ± 0.029	4.05AspSer: 4.05 ± 0.028	3.057AspThr: 3.057 ± 0.025	3.683AspVal: 3.683 ± 0.023	0.905AspTrp: 0.905 ± 0.011	1.718AspTyr: 1.718 ± 0.02	0.0AspXaa: 0.0 ± 0.0
Glu	5.269GluAla: 5.269 ± 0.039	0.654GluCys: 0.654 ± 0.012	4.014GluAsp: 4.014 ± 0.032	5.457GluGlu: 5.457 ± 0.053	1.915GluPhe: 1.915 ± 0.021	3.799GluGly: 3.799 ± 0.03	1.409GluHis: 1.409 ± 0.015	2.992GluIle: 2.992 ± 0.022	3.415GluLys: 3.415 ± 0.031	5.161GluLeu: 5.161 ± 0.039	1.453GluMet: 1.453 ± 0.018	2.207GluAsn: 2.207 ± 0.022	2.85GluPro: 2.85 ± 0.045	2.566GluGln: 2.566 ± 0.024	3.887GluArg: 3.887 ± 0.029	4.321GluSer: 4.321 ± 0.035	3.541GluThr: 3.541 ± 0.028	3.643GluVal: 3.643 ± 0.029	0.914GluTrp: 0.914 ± 0.012	1.759GluTyr: 1.759 ± 0.019	0.0GluXaa: 0.0 ± 0.0
Phe	2.983PheAla: 2.983 ± 0.024	0.588PheCys: 0.588 ± 0.011	2.245PheAsp: 2.245 ± 0.018	2.06PheGlu: 2.06 ± 0.021	1.622PhePhe: 1.622 ± 0.017	2.783PheGly: 2.783 ± 0.026	0.966PheHis: 0.966 ± 0.014	1.849PheIle: 1.849 ± 0.022	1.328PheLys: 1.328 ± 0.016	3.613PheLeu: 3.613 ± 0.03	0.775PheMet: 0.775 ± 0.013	1.392PheAsn: 1.392 ± 0.016	2.007PhePro: 2.007 ± 0.019	1.429PheGln: 1.429 ± 0.017	2.035PheArg: 2.035 ± 0.019	2.995PheSer: 2.995 ± 0.024	2.207PheThr: 2.207 ± 0.021	2.389PheVal: 2.389 ± 0.021	0.663PheTrp: 0.663 ± 0.011	1.168PheTyr: 1.168 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	5.211GlyAla: 5.211 ± 0.039	0.911GlyCys: 0.911 ± 0.013	3.65GlyAsp: 3.65 ± 0.029	3.639GlyGlu: 3.639 ± 0.028	2.768GlyPhe: 2.768 ± 0.024	5.401GlyGly: 5.401 ± 0.047	1.712GlyHis: 1.712 ± 0.018	3.562GlyIle: 3.562 ± 0.026	3.16GlyLys: 3.16 ± 0.027	6.231GlyLeu: 6.231 ± 0.036	1.582GlyMet: 1.582 ± 0.019	2.421GlyAsn: 2.421 ± 0.023	3.336GlyPro: 3.336 ± 0.026	2.538GlyGln: 2.538 ± 0.021	4.075GlyArg: 4.075 ± 0.03	5.641GlySer: 5.641 ± 0.041	3.969GlyThr: 3.969 ± 0.029	4.527GlyVal: 4.527 ± 0.031	1.182GlyTrp: 1.182 ± 0.015	2.239GlyTyr: 2.239 ± 0.021	0.0GlyXaa: 0.0 ± 0.0
His	1.924HisAla: 1.924 ± 0.019	0.364HisCys: 0.364 ± 0.009	1.336HisAsp: 1.336 ± 0.015	1.351HisGlu: 1.351 ± 0.016	0.931HisPhe: 0.931 ± 0.013	1.716HisGly: 1.716 ± 0.018	0.922HisHis: 0.922 ± 0.016	1.271HisIle: 1.271 ± 0.015	0.84HisLys: 0.84 ± 0.012	2.398HisLeu: 2.398 ± 0.022	0.51HisMet: 0.51 ± 0.009	0.865HisAsn: 0.865 ± 0.012	1.819HisPro: 1.819 ± 0.02	1.005HisGln: 1.005 ± 0.014	1.605HisArg: 1.605 ± 0.017	1.85HisSer: 1.85 ± 0.018	1.347HisThr: 1.347 ± 0.017	1.459HisVal: 1.459 ± 0.018	0.368HisTrp: 0.368 ± 0.008	0.752HisTyr: 0.752 ± 0.011	0.0HisXaa: 0.0 ± 0.0
Ile	4.22IleAla: 4.22 ± 0.029	0.813IleCys: 0.813 ± 0.011	2.842IleAsp: 2.842 ± 0.022	2.809IleGlu: 2.809 ± 0.022	2.049IlePhe: 2.049 ± 0.022	3.244IleGly: 3.244 ± 0.03	1.298IleHis: 1.298 ± 0.014	2.609IleIle: 2.609 ± 0.026	1.975IleLys: 1.975 ± 0.022	4.801IleLeu: 4.801 ± 0.032	1.052IleMet: 1.052 ± 0.015	1.8IleAsn: 1.8 ± 0.019	3.223IlePro: 3.223 ± 0.025	1.977IleGln: 1.977 ± 0.02	2.884IleArg: 2.884 ± 0.024	3.899IleSer: 3.899 ± 0.026	2.906IleThr: 2.906 ± 0.024	3.22IleVal: 3.22 ± 0.024	0.756IleTrp: 0.756 ± 0.011	1.525IleTyr: 1.525 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	3.821LysAla: 3.821 ± 0.035	0.474LysCys: 0.474 ± 0.009	2.51LysAsp: 2.51 ± 0.022	3.158LysGlu: 3.158 ± 0.03	1.296LysPhe: 1.296 ± 0.016	2.734LysGly: 2.734 ± 0.026	1.058LysHis: 1.058 ± 0.013	2.101LysIle: 2.101 ± 0.022	2.76LysLys: 2.76 ± 0.037	3.827LysLeu: 3.827 ± 0.032	0.898LysMet: 0.898 ± 0.012	1.556LysAsn: 1.556 ± 0.018	2.498LysPro: 2.498 ± 0.026	1.743LysGln: 1.743 ± 0.018	3.103LysArg: 3.103 ± 0.029	3.164LysSer: 3.164 ± 0.029	2.523LysThr: 2.523 ± 0.021	2.659LysVal: 2.659 ± 0.024	0.631LysTrp: 0.631 ± 0.009	1.328LysTyr: 1.328 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	7.981LeuAla: 7.981 ± 0.048	1.293LeuCys: 1.293 ± 0.016	5.259LeuAsp: 5.259 ± 0.034	5.566LeuGlu: 5.566 ± 0.042	3.463LeuPhe: 3.463 ± 0.03	6.11LeuGly: 6.11 ± 0.036	2.396LeuHis: 2.396 ± 0.021	4.125LeuIle: 4.125 ± 0.03	3.907LeuLys: 3.907 ± 0.031	8.939LeuLeu: 8.939 ± 0.058	1.857LeuMet: 1.857 ± 0.018	3.177LeuAsn: 3.177 ± 0.024	5.668LeuPro: 5.668 ± 0.034	4.038LeuGln: 4.038 ± 0.034	5.947LeuArg: 5.947 ± 0.041	7.619LeuSer: 7.619 ± 0.036	5.025LeuThr: 5.025 ± 0.035	5.625LeuVal: 5.625 ± 0.036	1.279LeuTrp: 1.279 ± 0.016	2.567LeuTyr: 2.567 ± 0.02	0.0LeuXaa: 0.0 ± 0.0
Met	2.166MetAla: 2.166 ± 0.021	0.245MetCys: 0.245 ± 0.007	1.234MetAsp: 1.234 ± 0.016	1.318MetGlu: 1.318 ± 0.018	0.754MetPhe: 0.754 ± 0.012	1.474MetGly: 1.474 ± 0.016	0.5MetHis: 0.5 ± 0.009	1.041MetIle: 1.041 ± 0.012	0.958MetLys: 0.958 ± 0.012	1.895MetLeu: 1.895 ± 0.018	0.565MetMet: 0.565 ± 0.01	0.794MetAsn: 0.794 ± 0.012	1.229MetPro: 1.229 ± 0.014	0.89MetGln: 0.89 ± 0.013	1.266MetArg: 1.266 ± 0.012	1.88MetSer: 1.88 ± 0.02	1.298MetThr: 1.298 ± 0.016	1.36MetVal: 1.36 ± 0.016	0.267MetTrp: 0.267 ± 0.007	0.539MetTyr: 0.539 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	3.029AsnAla: 3.029 ± 0.021	0.462AsnCys: 0.462 ± 0.009	1.885AsnAsp: 1.885 ± 0.019	1.985AsnGlu: 1.985 ± 0.02	1.315AsnPhe: 1.315 ± 0.013	2.85AsnGly: 2.85 ± 0.027	0.85AsnHis: 0.85 ± 0.012	2.052AsnIle: 2.052 ± 0.019	1.404AsnLys: 1.404 ± 0.018	3.21AsnLeu: 3.21 ± 0.025	0.835AsnMet: 0.835 ± 0.013	1.469AsnAsn: 1.469 ± 0.016	2.436AsnPro: 2.436 ± 0.022	1.323AsnGln: 1.323 ± 0.015	1.946AsnArg: 1.946 ± 0.018	2.635AsnSer: 2.635 ± 0.022	2.183AsnThr: 2.183 ± 0.02	2.32AsnVal: 2.32 ± 0.021	0.578AsnTrp: 0.578 ± 0.01	1.108AsnTyr: 1.108 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	5.188ProAla: 5.188 ± 0.049	0.568ProCys: 0.568 ± 0.012	3.289ProAsp: 3.289 ± 0.024	3.93ProGlu: 3.93 ± 0.038	2.11ProPhe: 2.11 ± 0.021	3.882ProGly: 3.882 ± 0.026	1.382ProHis: 1.382 ± 0.017	2.586ProIle: 2.586 ± 0.024	2.415ProLys: 2.415 ± 0.025	4.845ProLeu: 4.845 ± 0.031	1.073ProMet: 1.073 ± 0.016	2.158ProAsn: 2.158 ± 0.022	4.775ProPro: 4.775 ± 0.067	2.464ProGln: 2.464 ± 0.031	3.375ProArg: 3.375 ± 0.03	6.126ProSer: 6.126 ± 0.041	4.041ProThr: 4.041 ± 0.033	3.757ProVal: 3.757 ± 0.033	0.796ProTrp: 0.796 ± 0.011	1.584ProTyr: 1.584 ± 0.018	0.0ProXaa: 0.0 ± 0.0
Gln	3.439GlnAla: 3.439 ± 0.033	0.479GlnCys: 0.479 ± 0.01	2.049GlnAsp: 2.049 ± 0.02	2.472GlnGlu: 2.472 ± 0.022	1.325GlnPhe: 1.325 ± 0.015	2.45GlnGly: 2.45 ± 0.022	1.094GlnHis: 1.094 ± 0.016	1.922GlnIle: 1.922 ± 0.021	1.902GlnLys: 1.902 ± 0.022	3.672GlnLeu: 3.672 ± 0.027	0.886GlnMet: 0.886 ± 0.014	1.499GlnAsn: 1.499 ± 0.018	2.606GlnPro: 2.606 ± 0.032	2.468GlnGln: 2.468 ± 0.051	2.644GlnArg: 2.644 ± 0.021	3.208GlnSer: 3.208 ± 0.025	2.345GlnThr: 2.345 ± 0.021	2.259GlnVal: 2.259 ± 0.021	0.613GlnTrp: 0.613 ± 0.01	1.235GlnTyr: 1.235 ± 0.013	0.0GlnXaa: 0.0 ± 0.0
Arg	4.687ArgAla: 4.687 ± 0.036	0.765ArgCys: 0.765 ± 0.011	3.38ArgAsp: 3.38 ± 0.03	3.828ArgGlu: 3.828 ± 0.03	2.184ArgPhe: 2.184 ± 0.018	3.669ArgGly: 3.669 ± 0.028	1.547ArgHis: 1.547 ± 0.017	2.922ArgIle: 2.922 ± 0.024	3.231ArgLys: 3.231 ± 0.027	5.73ArgLeu: 5.73 ± 0.036	1.301ArgMet: 1.301 ± 0.015	2.189ArgAsn: 2.189 ± 0.019	3.495ArgPro: 3.495 ± 0.03	2.677ArgGln: 2.677 ± 0.021	4.986ArgArg: 4.986 ± 0.048	4.78ArgSer: 4.78 ± 0.039	3.29ArgThr: 3.29 ± 0.026	3.523ArgVal: 3.523 ± 0.026	0.965ArgTrp: 0.965 ± 0.014	1.773ArgTyr: 1.773 ± 0.018	0.0ArgXaa: 0.0 ± 0.0
Ser	6.701SerAla: 6.701 ± 0.042	0.896SerCys: 0.896 ± 0.014	4.307SerAsp: 4.307 ± 0.032	4.211SerGlu: 4.211 ± 0.029	3.016SerPhe: 3.016 ± 0.025	5.523SerGly: 5.523 ± 0.042	2.008SerHis: 2.008 ± 0.021	4.022SerIle: 4.022 ± 0.028	3.31SerLys: 3.31 ± 0.026	7.532SerLeu: 7.532 ± 0.038	1.717SerMet: 1.717 ± 0.017	2.916SerAsn: 2.916 ± 0.026	5.45SerPro: 5.45 ± 0.048	3.33SerGln: 3.33 ± 0.028	4.986SerArg: 4.986 ± 0.04	8.94SerSer: 8.94 ± 0.068	5.708SerThr: 5.708 ± 0.038	4.766SerVal: 4.766 ± 0.029	1.19SerTrp: 1.19 ± 0.015	2.172SerTyr: 2.172 ± 0.021	0.0SerXaa: 0.0 ± 0.0
Thr	5.28ThrAla: 5.28 ± 0.034	0.765ThrCys: 0.765 ± 0.011	2.997ThrAsp: 2.997 ± 0.024	3.222ThrGlu: 3.222 ± 0.028	2.229ThrPhe: 2.229 ± 0.02	4.312ThrGly: 4.312 ± 0.033	1.34ThrHis: 1.34 ± 0.017	3.151ThrIle: 3.151 ± 0.028	2.366ThrLys: 2.366 ± 0.021	5.46ThrLeu: 5.46 ± 0.033	1.227ThrMet: 1.227 ± 0.015	2.095ThrAsn: 2.095 ± 0.021	4.299ThrPro: 4.299 ± 0.038	2.127ThrGln: 2.127 ± 0.022	3.082ThrArg: 3.082 ± 0.024	5.354ThrSer: 5.354 ± 0.04	4.578ThrThr: 4.578 ± 0.046	3.936ThrVal: 3.936 ± 0.029	0.894ThrTrp: 0.894 ± 0.015	1.753ThrTyr: 1.753 ± 0.02	0.0ThrXaa: 0.0 ± 0.0
Val	5.291ValAla: 5.291 ± 0.034	0.876ValCys: 0.876 ± 0.014	3.796ValAsp: 3.796 ± 0.025	3.875ValGlu: 3.875 ± 0.033	2.506ValPhe: 2.506 ± 0.021	4.08ValGly: 4.08 ± 0.031	1.479ValHis: 1.479 ± 0.016	3.112ValIle: 3.112 ± 0.023	2.693ValLys: 2.693 ± 0.021	5.797ValLeu: 5.797 ± 0.035	1.364ValMet: 1.364 ± 0.015	2.28ValAsn: 2.28 ± 0.019	3.703ValPro: 3.703 ± 0.027	2.489ValGln: 2.489 ± 0.021	3.567ValArg: 3.567 ± 0.023	4.815ValSer: 4.815 ± 0.031	3.69ValThr: 3.69 ± 0.027	4.438ValVal: 4.438 ± 0.031	0.896ValTrp: 0.896 ± 0.014	1.875ValTyr: 1.875 ± 0.019	0.0ValXaa: 0.0 ± 0.0
Trp	1.161TrpAla: 1.161 ± 0.014	0.203TrpCys: 0.203 ± 0.006	0.921TrpAsp: 0.921 ± 0.013	0.873TrpGlu: 0.873 ± 0.012	0.552TrpPhe: 0.552 ± 0.01	0.963TrpGly: 0.963 ± 0.016	0.373TrpHis: 0.373 ± 0.008	0.804TrpIle: 0.804 ± 0.013	0.82TrpLys: 0.82 ± 0.011	1.424TrpLeu: 1.424 ± 0.016	0.375TrpMet: 0.375 ± 0.008	0.646TrpAsn: 0.646 ± 0.011	0.653TrpPro: 0.653 ± 0.011	0.591TrpGln: 0.591 ± 0.01	0.985TrpArg: 0.985 ± 0.014	1.096TrpSer: 1.096 ± 0.015	0.97TrpThr: 0.97 ± 0.015	0.95TrpVal: 0.95 ± 0.014	0.292TrpTrp: 0.292 ± 0.007	0.461TrpTyr: 0.461 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.28TyrAla: 2.28 ± 0.022	0.442TyrCys: 0.442 ± 0.008	1.691TyrAsp: 1.691 ± 0.018	1.592TyrGlu: 1.592 ± 0.017	1.221TyrPhe: 1.221 ± 0.015	2.179TyrGly: 2.179 ± 0.021	0.83TyrHis: 0.83 ± 0.013	1.506TyrIle: 1.506 ± 0.017	1.036TyrLys: 1.036 ± 0.015	2.887TyrLeu: 2.887 ± 0.02	0.667TyrMet: 0.667 ± 0.012	1.183TyrAsn: 1.183 ± 0.015	1.65TyrPro: 1.65 ± 0.02	1.165TyrGln: 1.165 ± 0.015	1.744TyrArg: 1.744 ± 0.017	2.127TyrSer: 2.127 ± 0.018	1.755TyrThr: 1.755 ± 0.019	1.743TyrVal: 1.743 ± 0.018	0.496TyrTrp: 0.496 ± 0.011	1.037TyrTyr: 1.037 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 11139 proteins (6005040 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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