Sphingobacterium sp. dk4302

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Sphingobacterium; unclassified Sphingobacterium

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3478 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q0QGT1|A0A5Q0QGT1_9SPHI Carboxypeptidase-like regulatory domain-containing protein OS=Sphingobacterium sp. dk4302 OX=2662364 GN=GFH32_11435 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.17HH3 pKa = 6.01QINTSEE9 pKa = 3.67ARR11 pKa = 11.84VYY13 pKa = 10.51VGTYY17 pKa = 9.68GKK19 pKa = 10.68YY20 pKa = 9.48NDD22 pKa = 3.91GSIFGKK28 pKa = 9.65WLDD31 pKa = 3.67LSDD34 pKa = 3.9YY35 pKa = 11.13ANSDD39 pKa = 3.43EE40 pKa = 6.01FYY42 pKa = 9.16TACKK46 pKa = 9.7EE47 pKa = 4.07LHH49 pKa = 6.45NDD51 pKa = 3.23EE52 pKa = 5.29ADD54 pKa = 3.6PEE56 pKa = 4.99FMFQDD61 pKa = 4.05YY62 pKa = 11.31EE63 pKa = 4.3NIPNGLIGEE72 pKa = 4.36SWISNNIFEE81 pKa = 4.62VLEE84 pKa = 4.02ALKK87 pKa = 11.23DD88 pKa = 3.45MDD90 pKa = 4.17EE91 pKa = 4.22SRR93 pKa = 11.84KK94 pKa = 9.42EE95 pKa = 4.55AFLIWCDD102 pKa = 3.13NGHH105 pKa = 6.56RR106 pKa = 11.84KK107 pKa = 9.7LSEE110 pKa = 3.86TDD112 pKa = 3.03ISDD115 pKa = 4.12LMSDD119 pKa = 4.7FDD121 pKa = 5.05DD122 pKa = 6.17DD123 pKa = 5.36YY124 pKa = 11.42IGEE127 pKa = 4.34YY128 pKa = 10.1KK129 pKa = 10.71DD130 pKa = 4.44EE131 pKa = 4.16EE132 pKa = 4.36DD133 pKa = 3.41FAYY136 pKa = 10.22EE137 pKa = 4.02QVEE140 pKa = 4.31QMDD143 pKa = 4.07LPEE146 pKa = 4.13FAKK149 pKa = 10.63QYY151 pKa = 10.87FDD153 pKa = 3.66YY154 pKa = 10.72EE155 pKa = 4.17AYY157 pKa = 10.44ARR159 pKa = 11.84DD160 pKa = 4.51LFSGDD165 pKa = 3.18YY166 pKa = 9.57WSDD169 pKa = 2.95SGYY172 pKa = 11.05VFYY175 pKa = 11.36NSS177 pKa = 3.31

Molecular weight: 20.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q0QIB4|A0A5Q0QIB4_9SPHI Acyltransferase family protein OS=Sphingobacterium sp. dk4302 OX=2662364 GN=GFH32_17145 PE=4 SV=1
MM1 pKa = 6.97QAGSKK6 pKa = 9.88FIQLMSRR13 pKa = 11.84LSRR16 pKa = 11.84RR17 pKa = 11.84IRR19 pKa = 11.84WNFKK23 pKa = 8.45QEE25 pKa = 3.73NRR27 pKa = 11.84FGSAGVARR35 pKa = 11.84AAFALLLLGLFLYY48 pKa = 10.65VVLDD52 pKa = 3.37SRR54 pKa = 11.84LYY56 pKa = 7.72PWEE59 pKa = 3.96YY60 pKa = 11.31ASLQRR65 pKa = 11.84ATGVALQQSAEE76 pKa = 3.92HH77 pKa = 6.76RR78 pKa = 11.84FQLRR82 pKa = 11.84AEE84 pKa = 4.05APEE87 pKa = 3.86NFRR90 pKa = 11.84IRR92 pKa = 11.84AFCRR96 pKa = 11.84YY97 pKa = 8.4MDD99 pKa = 4.56SLNSSPQGKK108 pKa = 7.91KK109 pKa = 8.73TYY111 pKa = 10.46DD112 pKa = 3.35SIMEE116 pKa = 4.15RR117 pKa = 11.84RR118 pKa = 11.84PGLLDD123 pKa = 3.18SARR126 pKa = 11.84NLLYY130 pKa = 10.44HH131 pKa = 6.05YY132 pKa = 10.63NN133 pKa = 3.58

Molecular weight: 15.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3478

0

3478

1255142

25

2009

360.9

40.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.253 ± 0.038

0.706 ± 0.011

5.499 ± 0.025

6.184 ± 0.037

4.935 ± 0.031

6.626 ± 0.038

1.845 ± 0.02

7.383 ± 0.036

6.973 ± 0.033

9.589 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.287 ± 0.017

5.692 ± 0.036

3.543 ± 0.02

3.933 ± 0.021

4.074 ± 0.025

6.687 ± 0.034

5.219 ± 0.027

6.227 ± 0.032

1.182 ± 0.014

4.162 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski