Amino acid dipepetide frequency for Sphingobacterium sp. dk4302

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.682AlaAla: 5.682 ± 0.085
0.625AlaCys: 0.625 ± 0.025
4.098AlaAsp: 4.098 ± 0.067
4.599AlaGlu: 4.599 ± 0.064
3.416AlaPhe: 3.416 ± 0.061
4.946AlaGly: 4.946 ± 0.068
1.205AlaHis: 1.205 ± 0.033
5.697AlaIle: 5.697 ± 0.084
5.165AlaLys: 5.165 ± 0.077
6.854AlaLeu: 6.854 ± 0.082
1.79AlaMet: 1.79 ± 0.037
4.035AlaAsn: 4.035 ± 0.059
2.031AlaPro: 2.031 ± 0.044
2.865AlaGln: 2.865 ± 0.047
2.472AlaArg: 2.472 ± 0.044
4.913AlaSer: 4.913 ± 0.065
3.684AlaThr: 3.684 ± 0.06
4.662AlaVal: 4.662 ± 0.056
0.79AlaTrp: 0.79 ± 0.025
2.997AlaTyr: 2.997 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
0.447CysAla: 0.447 ± 0.021
0.122CysCys: 0.122 ± 0.009
0.34CysAsp: 0.34 ± 0.017
0.357CysGlu: 0.357 ± 0.018
0.378CysPhe: 0.378 ± 0.018
0.558CysGly: 0.558 ± 0.026
0.176CysHis: 0.176 ± 0.013
0.57CysIle: 0.57 ± 0.024
0.425CysLys: 0.425 ± 0.018
0.73CysLeu: 0.73 ± 0.026
0.152CysMet: 0.152 ± 0.011
0.334CysAsn: 0.334 ± 0.017
0.265CysPro: 0.265 ± 0.017
0.249CysGln: 0.249 ± 0.014
0.276CysArg: 0.276 ± 0.015
0.539CysSer: 0.539 ± 0.022
0.372CysThr: 0.372 ± 0.018
0.394CysVal: 0.394 ± 0.017
0.082CysTrp: 0.082 ± 0.008
0.3CysTyr: 0.3 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
3.995AspAla: 3.995 ± 0.063
0.347AspCys: 0.347 ± 0.018
2.463AspAsp: 2.463 ± 0.047
3.508AspGlu: 3.508 ± 0.052
3.141AspPhe: 3.141 ± 0.062
3.774AspGly: 3.774 ± 0.06
1.031AspHis: 1.031 ± 0.028
4.186AspIle: 4.186 ± 0.059
3.921AspLys: 3.921 ± 0.061
5.458AspLeu: 5.458 ± 0.063
1.252AspMet: 1.252 ± 0.032
2.74AspAsn: 2.74 ± 0.053
2.115AspPro: 2.115 ± 0.039
2.298AspGln: 2.298 ± 0.046
2.438AspArg: 2.438 ± 0.05
3.121AspSer: 3.121 ± 0.051
2.263AspThr: 2.263 ± 0.048
3.47AspVal: 3.47 ± 0.059
0.8AspTrp: 0.8 ± 0.026
2.668AspTyr: 2.668 ± 0.05
0.0AspXaa: 0.0 ± 0.0
Glu
4.509GluAla: 4.509 ± 0.061
0.3GluCys: 0.3 ± 0.018
3.102GluAsp: 3.102 ± 0.051
4.516GluGlu: 4.516 ± 0.069
2.651GluPhe: 2.651 ± 0.053
3.78GluGly: 3.78 ± 0.053
1.241GluHis: 1.241 ± 0.036
4.794GluIle: 4.794 ± 0.071
4.844GluLys: 4.844 ± 0.064
6.367GluLeu: 6.367 ± 0.075
1.469GluMet: 1.469 ± 0.035
3.651GluAsn: 3.651 ± 0.056
1.556GluPro: 1.556 ± 0.035
2.765GluGln: 2.765 ± 0.051
2.881GluArg: 2.881 ± 0.055
3.514GluSer: 3.514 ± 0.054
2.973GluThr: 2.973 ± 0.051
4.102GluVal: 4.102 ± 0.065
0.684GluTrp: 0.684 ± 0.026
2.138GluTyr: 2.138 ± 0.048
0.0GluXaa: 0.0 ± 0.0
Phe
3.423PheAla: 3.423 ± 0.061
0.407PheCys: 0.407 ± 0.02
3.06PheAsp: 3.06 ± 0.054
3.037PheGlu: 3.037 ± 0.048
2.456PhePhe: 2.456 ± 0.053
3.49PheGly: 3.49 ± 0.067
0.851PheHis: 0.851 ± 0.026
3.393PheIle: 3.393 ± 0.059
3.149PheLys: 3.149 ± 0.049
4.458PheLeu: 4.458 ± 0.079
1.139PheMet: 1.139 ± 0.03
2.982PheAsn: 2.982 ± 0.061
1.825PhePro: 1.825 ± 0.04
1.842PheGln: 1.842 ± 0.037
1.942PheArg: 1.942 ± 0.039
3.602PheSer: 3.602 ± 0.057
2.636PheThr: 2.636 ± 0.049
3.032PheVal: 3.032 ± 0.054
0.584PheTrp: 0.584 ± 0.022
2.04PheTyr: 2.04 ± 0.041
0.0PheXaa: 0.0 ± 0.0
Gly
4.599GlyAla: 4.599 ± 0.072
0.512GlyCys: 0.512 ± 0.021
3.333GlyAsp: 3.333 ± 0.06
3.544GlyGlu: 3.544 ± 0.055
3.527GlyPhe: 3.527 ± 0.059
4.47GlyGly: 4.47 ± 0.08
1.163GlyHis: 1.163 ± 0.032
5.217GlyIle: 5.217 ± 0.078
5.036GlyLys: 5.036 ± 0.062
6.246GlyLeu: 6.246 ± 0.074
1.67GlyMet: 1.67 ± 0.033
3.665GlyAsn: 3.665 ± 0.059
1.493GlyPro: 1.493 ± 0.037
2.376GlyGln: 2.376 ± 0.046
2.681GlyArg: 2.681 ± 0.059
4.351GlySer: 4.351 ± 0.067
3.946GlyThr: 3.946 ± 0.055
4.359GlyVal: 4.359 ± 0.059
0.891GlyTrp: 0.891 ± 0.03
3.186GlyTyr: 3.186 ± 0.058
0.0GlyXaa: 0.0 ± 0.0
His
1.239HisAla: 1.239 ± 0.033
0.189HisCys: 0.189 ± 0.012
0.907HisAsp: 0.907 ± 0.028
0.982HisGlu: 0.982 ± 0.028
1.081HisPhe: 1.081 ± 0.033
1.14HisGly: 1.14 ± 0.031
0.479HisHis: 0.479 ± 0.021
1.48HisIle: 1.48 ± 0.036
1.002HisLys: 1.002 ± 0.028
1.829HisLeu: 1.829 ± 0.043
0.415HisMet: 0.415 ± 0.018
0.954HisAsn: 0.954 ± 0.032
0.943HisPro: 0.943 ± 0.028
0.843HisGln: 0.843 ± 0.026
0.793HisArg: 0.793 ± 0.025
1.115HisSer: 1.115 ± 0.031
0.843HisThr: 0.843 ± 0.029
1.094HisVal: 1.094 ± 0.03
0.258HisTrp: 0.258 ± 0.013
0.864HisTyr: 0.864 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
6.025IleAla: 6.025 ± 0.082
0.647IleCys: 0.647 ± 0.024
4.639IleAsp: 4.639 ± 0.069
4.647IleGlu: 4.647 ± 0.068
3.169IlePhe: 3.169 ± 0.063
5.042IleGly: 5.042 ± 0.075
1.401IleHis: 1.401 ± 0.035
5.018IleIle: 5.018 ± 0.065
4.797IleLys: 4.797 ± 0.066
6.643IleLeu: 6.643 ± 0.08
1.412IleMet: 1.412 ± 0.036
4.143IleAsn: 4.143 ± 0.055
3.307IlePro: 3.307 ± 0.048
2.902IleGln: 2.902 ± 0.046
3.079IleArg: 3.079 ± 0.051
5.259IleSer: 5.259 ± 0.063
3.693IleThr: 3.693 ± 0.053
4.552IleVal: 4.552 ± 0.063
0.736IleTrp: 0.736 ± 0.027
2.722IleTyr: 2.722 ± 0.051
0.0IleXaa: 0.0 ± 0.0
Lys
4.975LysAla: 4.975 ± 0.067
0.265LysCys: 0.265 ± 0.014
4.278LysAsp: 4.278 ± 0.06
5.207LysGlu: 5.207 ± 0.074
2.737LysPhe: 2.737 ± 0.047
4.468LysGly: 4.468 ± 0.06
1.352LysHis: 1.352 ± 0.033
4.985LysIle: 4.985 ± 0.068
5.05LysLys: 5.05 ± 0.075
6.239LysLeu: 6.239 ± 0.071
1.751LysMet: 1.751 ± 0.034
4.198LysAsn: 4.198 ± 0.057
2.537LysPro: 2.537 ± 0.044
2.843LysGln: 2.843 ± 0.049
2.923LysArg: 2.923 ± 0.041
4.295LysSer: 4.295 ± 0.059
3.815LysThr: 3.815 ± 0.059
4.238LysVal: 4.238 ± 0.058
0.793LysTrp: 0.793 ± 0.026
2.777LysTyr: 2.777 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
7.168LeuAla: 7.168 ± 0.094
0.745LeuCys: 0.745 ± 0.027
5.22LeuAsp: 5.22 ± 0.064
5.435LeuGlu: 5.435 ± 0.07
4.822LeuPhe: 4.822 ± 0.077
6.164LeuGly: 6.164 ± 0.073
1.689LeuHis: 1.689 ± 0.037
6.788LeuIle: 6.788 ± 0.089
6.967LeuLys: 6.967 ± 0.078
9.312LeuLeu: 9.312 ± 0.11
2.255LeuMet: 2.255 ± 0.045
5.578LeuAsn: 5.578 ± 0.072
3.809LeuPro: 3.809 ± 0.055
3.655LeuGln: 3.655 ± 0.063
3.886LeuArg: 3.886 ± 0.06
7.327LeuSer: 7.327 ± 0.075
5.181LeuThr: 5.181 ± 0.067
5.575LeuVal: 5.575 ± 0.078
0.884LeuTrp: 0.884 ± 0.028
3.432LeuTyr: 3.432 ± 0.06
0.0LeuXaa: 0.0 ± 0.0
Met
1.691MetAla: 1.691 ± 0.041
0.152MetCys: 0.152 ± 0.011
1.24MetAsp: 1.24 ± 0.03
1.46MetGlu: 1.46 ± 0.042
0.903MetPhe: 0.903 ± 0.029
1.506MetGly: 1.506 ± 0.032
0.42MetHis: 0.42 ± 0.02
1.511MetIle: 1.511 ± 0.035
1.907MetLys: 1.907 ± 0.039
2.22MetLeu: 2.22 ± 0.044
0.606MetMet: 0.606 ± 0.027
1.338MetAsn: 1.338 ± 0.029
0.928MetPro: 0.928 ± 0.031
0.926MetGln: 0.926 ± 0.026
1.103MetArg: 1.103 ± 0.027
1.58MetSer: 1.58 ± 0.04
1.192MetThr: 1.192 ± 0.028
1.275MetVal: 1.275 ± 0.039
0.196MetTrp: 0.196 ± 0.014
0.721MetTyr: 0.721 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
3.945AsnAla: 3.945 ± 0.057
0.299AsnCys: 0.299 ± 0.018
2.779AsnAsp: 2.779 ± 0.05
3.211AsnGlu: 3.211 ± 0.061
2.816AsnPhe: 2.816 ± 0.049
3.706AsnGly: 3.706 ± 0.057
0.997AsnHis: 0.997 ± 0.028
4.216AsnIle: 4.216 ± 0.067
3.93AsnLys: 3.93 ± 0.057
5.45AsnLeu: 5.45 ± 0.07
1.289AsnMet: 1.289 ± 0.031
3.246AsnAsn: 3.246 ± 0.061
2.804AsnPro: 2.804 ± 0.047
2.384AsnGln: 2.384 ± 0.047
2.545AsnArg: 2.545 ± 0.044
3.708AsnSer: 3.708 ± 0.066
3.023AsnThr: 3.023 ± 0.056
3.168AsnVal: 3.168 ± 0.064
0.791AsnTrp: 0.791 ± 0.028
2.613AsnTyr: 2.613 ± 0.055
0.0AsnXaa: 0.0 ± 0.0
Pro
2.412ProAla: 2.412 ± 0.047
0.196ProCys: 0.196 ± 0.013
2.071ProAsp: 2.071 ± 0.039
2.695ProGlu: 2.695 ± 0.052
1.861ProPhe: 1.861 ± 0.037
2.185ProGly: 2.185 ± 0.041
0.659ProHis: 0.659 ± 0.025
2.789ProIle: 2.789 ± 0.049
2.4ProLys: 2.4 ± 0.048
3.217ProLeu: 3.217 ± 0.054
0.76ProMet: 0.76 ± 0.025
2.205ProAsn: 2.205 ± 0.047
0.726ProPro: 0.726 ± 0.022
1.27ProGln: 1.27 ± 0.033
1.167ProArg: 1.167 ± 0.031
2.389ProSer: 2.389 ± 0.044
2.099ProThr: 2.099 ± 0.045
2.391ProVal: 2.391 ± 0.046
0.417ProTrp: 0.417 ± 0.019
1.524ProTyr: 1.524 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
2.936GlnAla: 2.936 ± 0.048
0.184GlnCys: 0.184 ± 0.012
1.992GlnAsp: 1.992 ± 0.035
2.691GlnGlu: 2.691 ± 0.053
1.793GlnPhe: 1.793 ± 0.039
2.316GlnGly: 2.316 ± 0.041
0.869GlnHis: 0.869 ± 0.028
2.953GlnIle: 2.953 ± 0.053
2.661GlnLys: 2.661 ± 0.047
3.862GlnLeu: 3.862 ± 0.061
0.852GlnMet: 0.852 ± 0.028
2.105GlnAsn: 2.105 ± 0.045
1.133GlnPro: 1.133 ± 0.03
2.114GlnGln: 2.114 ± 0.042
1.613GlnArg: 1.613 ± 0.037
2.463GlnSer: 2.463 ± 0.044
1.99GlnThr: 1.99 ± 0.037
2.676GlnVal: 2.676 ± 0.051
0.47GlnTrp: 0.47 ± 0.02
1.658GlnTyr: 1.658 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
2.722ArgAla: 2.722 ± 0.048
0.246ArgCys: 0.246 ± 0.015
2.125ArgAsp: 2.125 ± 0.047
2.485ArgGlu: 2.485 ± 0.048
2.159ArgPhe: 2.159 ± 0.04
2.377ArgGly: 2.377 ± 0.053
0.695ArgHis: 0.695 ± 0.021
3.305ArgIle: 3.305 ± 0.045
2.921ArgLys: 2.921 ± 0.052
4.006ArgLeu: 4.006 ± 0.052
1.069ArgMet: 1.069 ± 0.028
2.385ArgAsn: 2.385 ± 0.044
1.329ArgPro: 1.329 ± 0.033
1.41ArgGln: 1.41 ± 0.035
1.719ArgArg: 1.719 ± 0.039
2.489ArgSer: 2.489 ± 0.048
2.118ArgThr: 2.118 ± 0.039
2.612ArgVal: 2.612 ± 0.044
0.605ArgTrp: 0.605 ± 0.022
1.96ArgTyr: 1.96 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
4.545SerAla: 4.545 ± 0.069
0.604SerCys: 0.604 ± 0.02
3.451SerAsp: 3.451 ± 0.051
3.589SerGlu: 3.589 ± 0.047
3.758SerPhe: 3.758 ± 0.056
4.481SerGly: 4.481 ± 0.062
1.107SerHis: 1.107 ± 0.03
5.299SerIle: 5.299 ± 0.072
4.513SerLys: 4.513 ± 0.065
6.665SerLeu: 6.665 ± 0.088
1.434SerMet: 1.434 ± 0.033
3.864SerAsn: 3.864 ± 0.061
2.289SerPro: 2.289 ± 0.039
2.188SerGln: 2.188 ± 0.041
2.647SerArg: 2.647 ± 0.051
4.772SerSer: 4.772 ± 0.073
3.745SerThr: 3.745 ± 0.06
4.114SerVal: 4.114 ± 0.059
0.813SerTrp: 0.813 ± 0.029
2.994SerTyr: 2.994 ± 0.051
0.0SerXaa: 0.0 ± 0.0
Thr
4.051ThrAla: 4.051 ± 0.056
0.307ThrCys: 0.307 ± 0.018
2.946ThrAsp: 2.946 ± 0.051
2.981ThrGlu: 2.981 ± 0.052
2.68ThrPhe: 2.68 ± 0.045
4.0ThrGly: 4.0 ± 0.067
0.953ThrHis: 0.953 ± 0.027
3.919ThrIle: 3.919 ± 0.06
3.311ThrLys: 3.311 ± 0.054
5.068ThrLeu: 5.068 ± 0.062
0.987ThrMet: 0.987 ± 0.029
2.734ThrAsn: 2.734 ± 0.057
2.071ThrPro: 2.071 ± 0.044
1.826ThrGln: 1.826 ± 0.036
1.74ThrArg: 1.74 ± 0.04
3.434ThrSer: 3.434 ± 0.049
2.829ThrThr: 2.829 ± 0.051
3.513ThrVal: 3.513 ± 0.059
0.638ThrTrp: 0.638 ± 0.025
2.203ThrTyr: 2.203 ± 0.046
0.0ThrXaa: 0.0 ± 0.0
Val
4.461ValAla: 4.461 ± 0.072
0.505ValCys: 0.505 ± 0.021
3.859ValAsp: 3.859 ± 0.062
4.03ValGlu: 4.03 ± 0.064
3.078ValPhe: 3.078 ± 0.057
4.149ValGly: 4.149 ± 0.069
1.06ValHis: 1.06 ± 0.029
4.361ValIle: 4.361 ± 0.065
4.203ValLys: 4.203 ± 0.055
6.134ValLeu: 6.134 ± 0.076
1.378ValMet: 1.378 ± 0.039
3.53ValAsn: 3.53 ± 0.056
2.254ValPro: 2.254 ± 0.044
2.177ValGln: 2.177 ± 0.044
2.499ValArg: 2.499 ± 0.048
4.42ValSer: 4.42 ± 0.061
2.997ValThr: 2.997 ± 0.049
4.126ValVal: 4.126 ± 0.073
0.663ValTrp: 0.663 ± 0.026
2.384ValTyr: 2.384 ± 0.045
0.0ValXaa: 0.0 ± 0.0
Trp
0.771TrpAla: 0.771 ± 0.024
0.094TrpCys: 0.094 ± 0.009
0.676TrpAsp: 0.676 ± 0.023
0.694TrpGlu: 0.694 ± 0.027
0.545TrpPhe: 0.545 ± 0.023
0.839TrpGly: 0.839 ± 0.029
0.218TrpHis: 0.218 ± 0.014
0.8TrpIle: 0.8 ± 0.023
0.9TrpLys: 0.9 ± 0.027
1.104TrpLeu: 1.104 ± 0.028
0.365TrpMet: 0.365 ± 0.016
0.766TrpAsn: 0.766 ± 0.026
0.326TrpPro: 0.326 ± 0.015
0.487TrpGln: 0.487 ± 0.021
0.524TrpArg: 0.524 ± 0.021
0.707TrpSer: 0.707 ± 0.025
0.661TrpThr: 0.661 ± 0.024
0.65TrpVal: 0.65 ± 0.026
0.159TrpTrp: 0.159 ± 0.012
0.529TrpTyr: 0.529 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.931TyrAla: 2.931 ± 0.055
0.319TyrCys: 0.319 ± 0.016
2.408TyrAsp: 2.408 ± 0.048
2.172TyrGlu: 2.172 ± 0.046
2.38TyrPhe: 2.38 ± 0.048
2.871TyrGly: 2.871 ± 0.058
0.884TyrHis: 0.884 ± 0.026
2.552TyrIle: 2.552 ± 0.046
2.624TyrLys: 2.624 ± 0.045
4.03TyrLeu: 4.03 ± 0.067
0.829TyrMet: 0.829 ± 0.029
2.465TyrAsn: 2.465 ± 0.053
1.692TyrPro: 1.692 ± 0.035
1.913TyrGln: 1.913 ± 0.036
1.812TyrArg: 1.812 ± 0.043
2.873TyrSer: 2.873 ± 0.053
2.129TyrThr: 2.129 ± 0.049
2.264TyrVal: 2.264 ± 0.042
0.562TyrTrp: 0.562 ± 0.023
1.915TyrTyr: 1.915 ± 0.039
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3478 proteins (1255142 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski