Staphylococcus virus 55
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4ZB55|Q4ZB55_9CAUD ORF052 OS=Staphylococcus virus 55 OX=320845 PE=4 SV=1
MM1 pKa = 8.14 DD2 pKa = 3.98 NRR4 pKa = 11.84 EE5 pKa = 4.06 QIEE8 pKa = 3.92 QSVISASAYY17 pKa = 10.04 NGNDD21 pKa = 3.29 TEE23 pKa = 4.52 GLLKK27 pKa = 10.49 EE28 pKa = 4.44 IEE30 pKa = 4.24 DD31 pKa = 4.28 VYY33 pKa = 11.57 KK34 pKa = 10.64 KK35 pKa = 10.72 AQAFDD40 pKa = 4.66 EE41 pKa = 4.34 ILEE44 pKa = 4.2 GLPNAMQDD52 pKa = 3.36 ALKK55 pKa = 10.66 EE56 pKa = 4.16 DD57 pKa = 3.68 IYY59 pKa = 11.25 LDD61 pKa = 3.66 EE62 pKa = 5.14 AVGIMTSQVVYY73 pKa = 10.27 KK74 pKa = 10.8 YY75 pKa = 10.77 EE76 pKa = 3.89 EE77 pKa = 4.06 EE78 pKa = 4.28 QEE80 pKa = 3.93 NDD82 pKa = 2.92
Molecular weight: 9.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.123
IPC2_protein 3.872
IPC_protein 3.783
Toseland 3.605
ProMoST 3.859
Dawson 3.757
Bjellqvist 3.961
Wikipedia 3.656
Rodwell 3.63
Grimsley 3.528
Solomon 3.732
Lehninger 3.694
Nozaki 3.884
DTASelect 4.012
Thurlkill 3.656
EMBOSS 3.668
Sillero 3.897
Patrickios 3.198
IPC_peptide 3.745
IPC2_peptide 3.884
IPC2.peptide.svr19 3.857
Protein with the highest isoelectric point:
>tr|Q4ZB84|Q4ZB84_9CAUD ORF025 OS=Staphylococcus virus 55 OX=320845 PE=4 SV=1
MM1 pKa = 7.18 VVTTAIRR8 pKa = 11.84 KK9 pKa = 8.57 RR10 pKa = 11.84 KK11 pKa = 8.08 ARR13 pKa = 11.84 KK14 pKa = 8.03 RR15 pKa = 11.84 KK16 pKa = 8.33 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 6.94 RR20 pKa = 11.84 TKK22 pKa = 9.87 IKK24 pKa = 10.32 RR25 pKa = 11.84 KK26 pKa = 9.4 KK27 pKa = 9.89 LKK29 pKa = 10.35 KK30 pKa = 9.43 KK31 pKa = 10.3 QKK33 pKa = 9.7 LKK35 pKa = 11.08 KK36 pKa = 10.08 KK37 pKa = 9.61 MLIKK41 pKa = 9.65 TIKK44 pKa = 9.75 IIKK47 pKa = 9.22
Molecular weight: 5.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.484
IPC2_protein 11.184
IPC_protein 12.691
Toseland 12.881
ProMoST 13.349
Dawson 12.881
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.91
Grimsley 12.925
Solomon 13.364
Lehninger 13.261
Nozaki 12.881
DTASelect 12.852
Thurlkill 12.881
EMBOSS 13.364
Sillero 12.881
Patrickios 12.632
IPC_peptide 13.364
IPC2_peptide 12.34
IPC2.peptide.svr19 9.033
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
77
0
77
13879
35
989
180.2
20.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.894 ± 0.324
0.634 ± 0.109
5.959 ± 0.266
7.068 ± 0.417
4.366 ± 0.235
5.663 ± 0.36
1.945 ± 0.165
7.328 ± 0.247
9.021 ± 0.379
7.89 ± 0.319
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.767 ± 0.181
6.341 ± 0.31
2.846 ± 0.234
4.316 ± 0.19
4.136 ± 0.226
6.153 ± 0.269
6.276 ± 0.258
6.06 ± 0.272
1.131 ± 0.153
4.208 ± 0.315
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here