Lactococcus phage LL-H (Lactococcus delbrueckii bacteriophage LL-H)
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8SDH8|Q8SDH8_BPLLH Uncharacterized protein OS=Lactococcus phage LL-H OX=12348 PE=4 SV=2
MM1 pKa = 7.66 IDD3 pKa = 3.48 VEE5 pKa = 4.34 NKK7 pKa = 8.62 MVAEE11 pKa = 4.69 GNYY14 pKa = 10.5 EE15 pKa = 4.03 MTKK18 pKa = 9.17 QAYY21 pKa = 9.06 IEE23 pKa = 4.22 MYY25 pKa = 10.42 KK26 pKa = 9.36 MAYY29 pKa = 9.52 EE30 pKa = 3.98 VDD32 pKa = 3.81 SPEE35 pKa = 4.59 DD36 pKa = 3.78 EE37 pKa = 4.6 SEE39 pKa = 3.94 EE40 pKa = 5.21 AYY42 pKa = 8.86 WQYY45 pKa = 11.82 LMDD48 pKa = 4.77 QIDD51 pKa = 4.03 HH52 pKa = 6.81 IEE54 pKa = 4.03 PVRR57 pKa = 11.84 ACLDD61 pKa = 3.37 TCGFDD66 pKa = 4.75 SMEE69 pKa = 5.6 DD70 pKa = 3.17 MANWFRR76 pKa = 11.84 TANTADD82 pKa = 3.65 LHH84 pKa = 7.15 DD85 pKa = 5.17 LLDD88 pKa = 4.54 DD89 pKa = 4.36 LEE91 pKa = 4.66 EE92 pKa = 4.21 ATEE95 pKa = 3.98
Molecular weight: 11.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.764
IPC2_protein 3.745
IPC_protein 3.706
Toseland 3.503
ProMoST 3.834
Dawson 3.681
Bjellqvist 3.884
Wikipedia 3.592
Rodwell 3.528
Grimsley 3.414
Solomon 3.668
Lehninger 3.617
Nozaki 3.808
DTASelect 3.973
Thurlkill 3.554
EMBOSS 3.605
Sillero 3.821
Patrickios 0.604
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.772
Protein with the highest isoelectric point:
>tr|Q38343|Q38343_BPLLH Uncharacterized protein OS=Lactococcus phage LL-H OX=12348 PE=4 SV=1
MM1 pKa = 7.78 SDD3 pKa = 3.36 LSVFSRR9 pKa = 11.84 MAQSTGSRR17 pKa = 11.84 SVRR20 pKa = 11.84 LQVLNQMHH28 pKa = 6.54 QDD30 pKa = 3.28 MEE32 pKa = 4.47 QYY34 pKa = 9.53 VPKK37 pKa = 10.44 RR38 pKa = 11.84 AGFLRR43 pKa = 11.84 SQSFVNDD50 pKa = 3.39 TGIHH54 pKa = 4.4 YY55 pKa = 6.41 TAKK58 pKa = 9.45 YY59 pKa = 10.64 ARR61 pKa = 11.84 AQFYY65 pKa = 10.86 GFVNGHH71 pKa = 5.43 RR72 pKa = 11.84 VRR74 pKa = 11.84 NYY76 pKa = 7.21 STPGTGRR83 pKa = 11.84 RR84 pKa = 11.84 WDD86 pKa = 3.83 LKK88 pKa = 11.03 AKK90 pKa = 10.17 AVYY93 pKa = 9.65 KK94 pKa = 10.76 ADD96 pKa = 3.46 WQKK99 pKa = 10.83 VAVAAFLKK107 pKa = 9.6 EE108 pKa = 4.23 AKK110 pKa = 10.32 GEE112 pKa = 4.19 YY113 pKa = 10.11
Molecular weight: 12.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.259
IPC2_protein 9.78
IPC_protein 10.218
Toseland 10.262
ProMoST 10.014
Dawson 10.467
Bjellqvist 10.16
Wikipedia 10.657
Rodwell 10.818
Grimsley 10.54
Solomon 10.496
Lehninger 10.467
Nozaki 10.218
DTASelect 10.16
Thurlkill 10.306
EMBOSS 10.657
Sillero 10.365
Patrickios 10.467
IPC_peptide 10.496
IPC2_peptide 8.873
IPC2.peptide.svr19 8.727
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51
0
51
10332
65
796
202.6
22.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.304 ± 0.354
0.755 ± 0.136
6.543 ± 0.301
5.507 ± 0.382
3.494 ± 0.216
7.123 ± 0.352
1.858 ± 0.14
5.846 ± 0.273
7.811 ± 0.382
7.365 ± 0.297
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.458 ± 0.174
5.217 ± 0.274
3.291 ± 0.189
4.21 ± 0.32
4.355 ± 0.272
6.794 ± 0.37
6.93 ± 0.375
6.446 ± 0.293
1.529 ± 0.142
4.162 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here