Caulobacter virus phiCbK
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 318 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J3SL11|J3SL11_9CAUD Uncharacterized protein OS=Caulobacter virus phiCbK OX=1204537 GN=CbK_gp058 PE=4 SV=1
MM1 pKa = 7.89 RR2 pKa = 11.84 EE3 pKa = 3.81 GFMLEE8 pKa = 4.36 GYY10 pKa = 9.94 GGLKK14 pKa = 9.79 IGPGGGADD22 pKa = 3.82 PYY24 pKa = 8.94 TTLGDD29 pKa = 4.04 GGSEE33 pKa = 3.97 GCIPVKK39 pKa = 10.77 DD40 pKa = 5.34 DD41 pKa = 3.22 IAPDD45 pKa = 3.55 GGTEE49 pKa = 3.9 IISPHH54 pKa = 6.43 GYY56 pKa = 9.87 GG57 pKa = 4.0
Molecular weight: 5.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.867
IPC2_protein 4.177
IPC_protein 4.012
Toseland 3.834
ProMoST 4.177
Dawson 3.999
Bjellqvist 4.164
Wikipedia 3.948
Rodwell 3.859
Grimsley 3.757
Solomon 3.973
Lehninger 3.935
Nozaki 4.139
DTASelect 4.317
Thurlkill 3.897
EMBOSS 3.948
Sillero 4.139
Patrickios 1.952
IPC_peptide 3.973
IPC2_peptide 4.113
IPC2.peptide.svr19 4.027
Protein with the highest isoelectric point:
>tr|J3SKX6|J3SKX6_9CAUD Uncharacterized protein OS=Caulobacter virus phiCbK OX=1204537 GN=CbK_gp190 PE=4 SV=1
MM1 pKa = 7.46 SSSEE5 pKa = 4.23 VLVQDD10 pKa = 4.79 PLPPAAPNRR19 pKa = 11.84 RR20 pKa = 11.84 HH21 pKa = 6.59 RR22 pKa = 11.84 LFLEE26 pKa = 4.17 AGGLCTYY33 pKa = 8.65 CTGQMAYY40 pKa = 9.87 RR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 IGRR46 pKa = 11.84 GGGKK50 pKa = 9.88 LEE52 pKa = 4.32 PDD54 pKa = 3.44 EE55 pKa = 4.17 VTIEE59 pKa = 4.04 HH60 pKa = 6.63 LEE62 pKa = 3.84 ARR64 pKa = 11.84 AEE66 pKa = 4.25 GGSNHH71 pKa = 6.27 EE72 pKa = 4.65 SNLAAACVACNNTKK86 pKa = 10.81 DD87 pKa = 3.64 NAMPAWEE94 pKa = 4.18 FRR96 pKa = 11.84 RR97 pKa = 11.84 LRR99 pKa = 11.84 LRR101 pKa = 11.84 LLPEE105 pKa = 4.32 WPPCTFPPLSVRR117 pKa = 11.84 RR118 pKa = 11.84 RR119 pKa = 11.84 EE120 pKa = 3.87 PGLRR124 pKa = 11.84 PVSKK128 pKa = 10.59 RR129 pKa = 11.84 IGRR132 pKa = 11.84 LFEE135 pKa = 5.85 NIDD138 pKa = 3.29 NPGYY142 pKa = 7.46 WRR144 pKa = 11.84 RR145 pKa = 11.84 WFGSDD150 pKa = 3.09 PTRR153 pKa = 11.84 KK154 pKa = 9.3 VALVLHH160 pKa = 6.58 RR161 pKa = 11.84 EE162 pKa = 3.96 QRR164 pKa = 11.84 HH165 pKa = 4.45 LAIKK169 pKa = 10.3 RR170 pKa = 11.84 RR171 pKa = 11.84 VLLEE175 pKa = 3.91 HH176 pKa = 6.82 LPGRR180 pKa = 11.84 NDD182 pKa = 2.81 SRR184 pKa = 11.84 TVVFWRR190 pKa = 11.84 FGPLSAIKK198 pKa = 10.53 VITGRR203 pKa = 11.84 SAAYY207 pKa = 9.55 EE208 pKa = 4.03 DD209 pKa = 4.14 RR210 pKa = 11.84 DD211 pKa = 4.04 LVLSLSWVWRR221 pKa = 11.84 GADD224 pKa = 3.45 VLTALLGRR232 pKa = 11.84 ARR234 pKa = 11.84 SAEE237 pKa = 4.13 TTPGGEE243 pKa = 4.09 PP244 pKa = 3.31
Molecular weight: 27.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.341
IPC_protein 10.204
Toseland 10.394
ProMoST 10.175
Dawson 10.526
Bjellqvist 10.292
Wikipedia 10.76
Rodwell 10.555
Grimsley 10.584
Solomon 10.657
Lehninger 10.613
Nozaki 10.438
DTASelect 10.262
Thurlkill 10.423
EMBOSS 10.804
Sillero 10.467
Patrickios 10.248
IPC_peptide 10.657
IPC2_peptide 9.589
IPC2.peptide.svr19 8.505
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
318
0
318
62441
24
2799
196.4
21.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.653 ± 0.225
0.918 ± 0.061
6.555 ± 0.157
6.275 ± 0.215
3.68 ± 0.112
8.285 ± 0.508
2.138 ± 0.111
4.606 ± 0.109
4.435 ± 0.219
8.326 ± 0.176
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.252 ± 0.09
2.92 ± 0.109
5.325 ± 0.155
3.181 ± 0.096
6.898 ± 0.246
4.937 ± 0.167
5.969 ± 0.276
6.914 ± 0.124
1.722 ± 0.079
3.014 ± 0.107
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here