Proteome-pI 2.0 - Proteome Isoelectric Point Database

Methanomassiliicoccales archaeon RumEn M2

Taxonomy: cellular organisms; Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Methanomassiliicoccales; unclassified Methanomassiliicoccales

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1175 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A0Q4BBW1|A0A0Q4BBW1_9ARCH Modification methylase HemK OS=Methanomassiliicoccales archaeon RumEn M2 OX=1713725 GN=AOA81_03555 PE=4 SV=1

MM1 pKa = 7.2GVADD5 pKa = 3.6ITLTAVWSLPAIPNAEE21 pKa = 4.06EE22 pKa = 4.14TVEE25 pKa = 4.1VAYY28 pKa = 11.0SSVNFSEE35 pKa = 4.35NHH37 pKa = 4.96VEE39 pKa = 3.73PDD41 pKa = 3.1KK42 pKa = 11.04EE43 pKa = 4.24YY44 pKa = 11.41SFGLEE49 pKa = 3.84NGVMARR55 pKa = 11.84FSGNDD60 pKa = 2.76ITGDD64 pKa = 3.54VTFTAISWDD73 pKa = 3.95GEE75 pKa = 4.12FHH77 pKa = 7.31VYY79 pKa = 10.04GADD82 pKa = 3.36VYY84 pKa = 11.04SIDD87 pKa = 3.78MVGSGSVNILIPVDD101 pKa = 3.52KK102 pKa = 10.5FGSPVVHH109 pKa = 7.17HH110 pKa = 7.22IDD112 pKa = 4.45AEE114 pKa = 4.57DD115 pKa = 3.82NDD117 pKa = 4.24VVLTSTIVEE126 pKa = 4.08IGGVKK131 pKa = 9.91YY132 pKa = 10.96ASFDD136 pKa = 3.55ADD138 pKa = 3.63NFSLFYY144 pKa = 10.76VVEE147 pKa = 4.98DD148 pKa = 4.27GSTEE152 pKa = 4.02GGPAGTNTNIMVGVIAIAIAAILVAVWAYY181 pKa = 10.31RR182 pKa = 11.84RR183 pKa = 11.84NALL186 pKa = 4.18

Molecular weight: 19.79 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A0Q4B072|A0A0Q4B072_9ARCH Uncharacterized protein OS=Methanomassiliicoccales archaeon RumEn M2 OX=1713725 GN=AOA81_06335 PE=4 SV=1

MM1 pKa = 7.57TNNKK5 pKa = 8.62PPAMKK10 pKa = 10.42ARR12 pKa = 11.84LNKK15 pKa = 10.06AGKK18 pKa = 7.8QNRR21 pKa = 11.84RR22 pKa = 11.84VPAWVMMRR30 pKa = 11.84TNRR33 pKa = 11.84QFLRR37 pKa = 11.84HH38 pKa = 5.85PKK40 pKa = 8.66RR41 pKa = 11.84RR42 pKa = 11.84SWRR45 pKa = 11.84MTSLKK50 pKa = 10.34EE51 pKa = 3.78

Molecular weight: 6.21 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1175	0	1175	312200	39	1188	265.7	29.35

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
8.131 ± 0.078	1.489 ± 0.037	6.405 ± 0.052	7.026 ± 0.065	3.716 ± 0.045	8.177 ± 0.062	1.627 ± 0.03	6.97 ± 0.049	5.65 ± 0.075	8.423 ± 0.073

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
3.65 ± 0.041	3.294 ± 0.037	4.024 ± 0.038	1.901 ± 0.03	5.913 ± 0.066	6.84 ± 0.07	5.119 ± 0.063	7.607 ± 0.054	0.889 ± 0.023	3.149 ± 0.045

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski