Amino acid dipepetide frequency for Methanomassiliicoccales archaeon RumEn M2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.944AlaAla: 7.944 ± 0.231
1.054AlaCys: 1.054 ± 0.061
5.404AlaAsp: 5.404 ± 0.133
6.384AlaGlu: 6.384 ± 0.146
2.972AlaPhe: 2.972 ± 0.13
6.525AlaGly: 6.525 ± 0.167
1.188AlaHis: 1.188 ± 0.061
5.173AlaIle: 5.173 ± 0.136
4.5AlaLys: 4.5 ± 0.141
7.697AlaLeu: 7.697 ± 0.172
3.049AlaMet: 3.049 ± 0.101
2.213AlaAsn: 2.213 ± 0.082
2.508AlaPro: 2.508 ± 0.093
1.496AlaGln: 1.496 ± 0.065
3.908AlaArg: 3.908 ± 0.124
4.965AlaSer: 4.965 ± 0.126
3.434AlaThr: 3.434 ± 0.113
7.774AlaVal: 7.774 ± 0.196
0.641AlaTrp: 0.641 ± 0.048
2.479AlaTyr: 2.479 ± 0.099
0.0AlaXaa: 0.0 ± 0.0
Cys
1.163CysAla: 1.163 ± 0.06
0.285CysCys: 0.285 ± 0.031
0.922CysAsp: 0.922 ± 0.063
0.897CysGlu: 0.897 ± 0.053
0.484CysPhe: 0.484 ± 0.038
1.807CysGly: 1.807 ± 0.086
0.346CysHis: 0.346 ± 0.033
0.958CysIle: 0.958 ± 0.062
0.631CysLys: 0.631 ± 0.042
1.006CysLeu: 1.006 ± 0.067
0.426CysMet: 0.426 ± 0.035
0.439CysAsn: 0.439 ± 0.042
0.922CysPro: 0.922 ± 0.058
0.25CysGln: 0.25 ± 0.028
1.025CysArg: 1.025 ± 0.061
0.999CysSer: 0.999 ± 0.086
0.916CysThr: 0.916 ± 0.093
0.916CysVal: 0.916 ± 0.06
0.115CysTrp: 0.115 ± 0.018
0.381CysTyr: 0.381 ± 0.036
0.0CysXaa: 0.0 ± 0.0
Asp
5.471AspAla: 5.471 ± 0.146
0.884AspCys: 0.884 ± 0.05
3.578AspAsp: 3.578 ± 0.123
4.158AspGlu: 4.158 ± 0.119
2.498AspPhe: 2.498 ± 0.091
5.051AspGly: 5.051 ± 0.117
1.163AspHis: 1.163 ± 0.066
4.955AspIle: 4.955 ± 0.142
3.027AspLys: 3.027 ± 0.104
6.102AspLeu: 6.102 ± 0.168
2.306AspMet: 2.306 ± 0.087
1.947AspAsn: 1.947 ± 0.078
3.28AspPro: 3.28 ± 0.115
1.035AspGln: 1.035 ± 0.058
3.882AspArg: 3.882 ± 0.12
4.084AspSer: 4.084 ± 0.125
2.998AspThr: 2.998 ± 0.104
4.917AspVal: 4.917 ± 0.114
0.567AspTrp: 0.567 ± 0.045
2.146AspTyr: 2.146 ± 0.085
0.0AspXaa: 0.0 ± 0.0
Glu
5.753GluAla: 5.753 ± 0.148
1.089GluCys: 1.089 ± 0.055
4.263GluAsp: 4.263 ± 0.128
5.689GluGlu: 5.689 ± 0.175
2.479GluPhe: 2.479 ± 0.099
5.25GluGly: 5.25 ± 0.142
1.246GluHis: 1.246 ± 0.063
5.516GluIle: 5.516 ± 0.137
4.67GluLys: 4.67 ± 0.139
5.493GluLeu: 5.493 ± 0.129
2.703GluMet: 2.703 ± 0.085
2.55GluAsn: 2.55 ± 0.085
2.265GluPro: 2.265 ± 0.094
1.192GluGln: 1.192 ± 0.077
4.513GluArg: 4.513 ± 0.13
4.286GluSer: 4.286 ± 0.119
3.456GluThr: 3.456 ± 0.118
4.622GluVal: 4.622 ± 0.137
0.685GluTrp: 0.685 ± 0.043
2.543GluTyr: 2.543 ± 0.085
0.0GluXaa: 0.0 ± 0.0
Phe
2.739PheAla: 2.739 ± 0.09
0.602PheCys: 0.602 ± 0.047
2.777PheAsp: 2.777 ± 0.086
2.383PheGlu: 2.383 ± 0.094
1.63PhePhe: 1.63 ± 0.084
3.258PheGly: 3.258 ± 0.108
0.583PheHis: 0.583 ± 0.044
2.383PheIle: 2.383 ± 0.109
1.595PheLys: 1.595 ± 0.079
3.168PheLeu: 3.168 ± 0.129
1.204PheMet: 1.204 ± 0.062
1.118PheAsn: 1.118 ± 0.058
1.47PhePro: 1.47 ± 0.081
0.679PheGln: 0.679 ± 0.047
2.306PheArg: 2.306 ± 0.089
2.94PheSer: 2.94 ± 0.102
1.81PheThr: 1.81 ± 0.076
2.924PheVal: 2.924 ± 0.102
0.423PheTrp: 0.423 ± 0.046
1.166PheTyr: 1.166 ± 0.064
0.0PheXaa: 0.0 ± 0.0
Gly
6.169GlyAla: 6.169 ± 0.177
1.467GlyCys: 1.467 ± 0.076
4.734GlyAsp: 4.734 ± 0.132
4.917GlyGlu: 4.917 ± 0.131
3.065GlyPhe: 3.065 ± 0.098
6.134GlyGly: 6.134 ± 0.188
1.432GlyHis: 1.432 ± 0.086
6.393GlyIle: 6.393 ± 0.165
5.551GlyLys: 5.551 ± 0.148
6.63GlyLeu: 6.63 ± 0.161
3.232GlyMet: 3.232 ± 0.101
2.915GlyAsn: 2.915 ± 0.091
2.578GlyPro: 2.578 ± 0.096
1.432GlyGln: 1.432 ± 0.075
5.032GlyArg: 5.032 ± 0.146
5.525GlySer: 5.525 ± 0.155
4.958GlyThr: 4.958 ± 0.14
5.826GlyVal: 5.826 ± 0.13
0.887GlyTrp: 0.887 ± 0.053
2.896GlyTyr: 2.896 ± 0.097
0.0GlyXaa: 0.0 ± 0.0
His
1.127HisAla: 1.127 ± 0.057
0.327HisCys: 0.327 ± 0.035
1.015HisAsp: 1.015 ± 0.074
0.89HisGlu: 0.89 ± 0.048
0.65HisPhe: 0.65 ± 0.046
1.377HisGly: 1.377 ± 0.074
0.384HisHis: 0.384 ± 0.039
1.15HisIle: 1.15 ± 0.054
0.682HisLys: 0.682 ± 0.046
1.515HisLeu: 1.515 ± 0.071
0.615HisMet: 0.615 ± 0.048
0.493HisAsn: 0.493 ± 0.046
0.964HisPro: 0.964 ± 0.06
0.368HisGln: 0.368 ± 0.034
0.977HisArg: 0.977 ± 0.055
1.031HisSer: 1.031 ± 0.059
0.842HisThr: 0.842 ± 0.058
1.201HisVal: 1.201 ± 0.062
0.131HisTrp: 0.131 ± 0.022
0.532HisTyr: 0.532 ± 0.041
0.0HisXaa: 0.0 ± 0.0
Ile
5.695IleAla: 5.695 ± 0.147
1.073IleCys: 1.073 ± 0.058
4.475IleAsp: 4.475 ± 0.13
4.577IleGlu: 4.577 ± 0.128
2.466IlePhe: 2.466 ± 0.103
5.964IleGly: 5.964 ± 0.16
1.06IleHis: 1.06 ± 0.057
4.667IleIle: 4.667 ± 0.143
3.296IleLys: 3.296 ± 0.115
6.211IleLeu: 6.211 ± 0.143
2.316IleMet: 2.316 ± 0.094
2.191IleAsn: 2.191 ± 0.082
3.318IlePro: 3.318 ± 0.113
1.339IleGln: 1.339 ± 0.065
4.343IleArg: 4.343 ± 0.119
5.782IleSer: 5.782 ± 0.123
3.53IleThr: 3.53 ± 0.115
4.955IleVal: 4.955 ± 0.124
0.564IleTrp: 0.564 ± 0.044
1.877IleTyr: 1.877 ± 0.078
0.0IleXaa: 0.0 ± 0.0
Lys
5.103LysAla: 5.103 ± 0.135
0.737LysCys: 0.737 ± 0.055
4.19LysAsp: 4.19 ± 0.138
5.003LysGlu: 5.003 ± 0.144
1.365LysPhe: 1.365 ± 0.073
4.712LysGly: 4.712 ± 0.123
0.91LysHis: 0.91 ± 0.056
3.603LysIle: 3.603 ± 0.12
4.001LysLys: 4.001 ± 0.132
3.828LysLeu: 3.828 ± 0.132
1.909LysMet: 1.909 ± 0.082
2.14LysAsn: 2.14 ± 0.08
1.858LysPro: 1.858 ± 0.088
1.131LysGln: 1.131 ± 0.069
3.479LysArg: 3.479 ± 0.104
3.11LysSer: 3.11 ± 0.106
3.021LysThr: 3.021 ± 0.104
4.225LysVal: 4.225 ± 0.118
0.461LysTrp: 0.461 ± 0.035
1.714LysTyr: 1.714 ± 0.077
0.0LysXaa: 0.0 ± 0.0
Leu
6.759LeuAla: 6.759 ± 0.159
1.23LeuCys: 1.23 ± 0.058
5.551LeuAsp: 5.551 ± 0.138
5.727LeuGlu: 5.727 ± 0.162
3.312LeuPhe: 3.312 ± 0.139
6.672LeuGly: 6.672 ± 0.156
1.166LeuHis: 1.166 ± 0.062
5.605LeuIle: 5.605 ± 0.156
4.718LeuLys: 4.718 ± 0.14
7.044LeuLeu: 7.044 ± 0.207
3.434LeuMet: 3.434 ± 0.113
2.828LeuAsn: 2.828 ± 0.095
3.418LeuPro: 3.418 ± 0.116
1.579LeuGln: 1.579 ± 0.078
5.49LeuArg: 5.49 ± 0.144
6.672LeuSer: 6.672 ± 0.156
4.366LeuThr: 4.366 ± 0.118
5.653LeuVal: 5.653 ± 0.159
0.708LeuTrp: 0.708 ± 0.047
2.3LeuTyr: 2.3 ± 0.099
0.0LeuXaa: 0.0 ± 0.0
Met
3.283MetAla: 3.283 ± 0.103
0.429MetCys: 0.429 ± 0.036
2.357MetAsp: 2.357 ± 0.09
2.546MetGlu: 2.546 ± 0.083
1.201MetPhe: 1.201 ± 0.067
2.896MetGly: 2.896 ± 0.095
0.609MetHis: 0.609 ± 0.048
2.537MetIle: 2.537 ± 0.092
2.223MetLys: 2.223 ± 0.095
2.992MetLeu: 2.992 ± 0.101
1.39MetMet: 1.39 ± 0.068
1.256MetAsn: 1.256 ± 0.066
1.512MetPro: 1.512 ± 0.059
0.746MetGln: 0.746 ± 0.048
2.165MetArg: 2.165 ± 0.087
2.578MetSer: 2.578 ± 0.086
2.124MetThr: 2.124 ± 0.081
2.553MetVal: 2.553 ± 0.082
0.243MetTrp: 0.243 ± 0.03
0.858MetTyr: 0.858 ± 0.05
0.0MetXaa: 0.0 ± 0.0
Asn
2.572AsnAla: 2.572 ± 0.082
0.493AsnCys: 0.493 ± 0.038
1.771AsnAsp: 1.771 ± 0.073
2.028AsnGlu: 2.028 ± 0.074
0.977AsnPhe: 0.977 ± 0.051
2.883AsnGly: 2.883 ± 0.108
0.506AsnHis: 0.506 ± 0.042
2.553AsnIle: 2.553 ± 0.086
1.614AsnLys: 1.614 ± 0.08
2.963AsnLeu: 2.963 ± 0.102
1.326AsnMet: 1.326 ± 0.068
1.051AsnAsn: 1.051 ± 0.068
1.781AsnPro: 1.781 ± 0.074
0.577AsnGln: 0.577 ± 0.044
1.957AsnArg: 1.957 ± 0.084
2.008AsnSer: 2.008 ± 0.089
1.525AsnThr: 1.525 ± 0.067
2.944AsnVal: 2.944 ± 0.09
0.317AsnTrp: 0.317 ± 0.033
1.099AsnTyr: 1.099 ± 0.065
0.0AsnXaa: 0.0 ± 0.0
Pro
2.928ProAla: 2.928 ± 0.1
0.554ProCys: 0.554 ± 0.045
3.053ProAsp: 3.053 ± 0.107
3.786ProGlu: 3.786 ± 0.112
1.791ProPhe: 1.791 ± 0.084
3.584ProGly: 3.584 ± 0.115
0.657ProHis: 0.657 ± 0.05
2.14ProIle: 2.14 ± 0.084
2.287ProLys: 2.287 ± 0.103
3.302ProLeu: 3.302 ± 0.111
1.275ProMet: 1.275 ± 0.065
1.227ProAsn: 1.227 ± 0.061
1.393ProPro: 1.393 ± 0.068
0.906ProGln: 0.906 ± 0.053
1.877ProArg: 1.877 ± 0.078
2.649ProSer: 2.649 ± 0.098
1.787ProThr: 1.787 ± 0.088
3.424ProVal: 3.424 ± 0.104
0.375ProTrp: 0.375 ± 0.037
1.243ProTyr: 1.243 ± 0.064
0.0ProXaa: 0.0 ± 0.0
Gln
1.518GlnAla: 1.518 ± 0.079
0.247GlnCys: 0.247 ± 0.025
1.041GlnAsp: 1.041 ± 0.059
1.185GlnGlu: 1.185 ± 0.071
0.615GlnPhe: 0.615 ± 0.044
1.294GlnGly: 1.294 ± 0.06
0.301GlnHis: 0.301 ± 0.028
1.496GlnIle: 1.496 ± 0.08
1.284GlnLys: 1.284 ± 0.062
1.384GlnLeu: 1.384 ± 0.062
0.958GlnMet: 0.958 ± 0.053
0.708GlnAsn: 0.708 ± 0.045
0.612GlnPro: 0.612 ± 0.042
0.5GlnGln: 0.5 ± 0.046
1.22GlnArg: 1.22 ± 0.058
1.358GlnSer: 1.358 ± 0.067
1.111GlnThr: 1.111 ± 0.064
1.23GlnVal: 1.23 ± 0.063
0.227GlnTrp: 0.227 ± 0.029
0.721GlnTyr: 0.721 ± 0.046
0.0GlnXaa: 0.0 ± 0.0
Arg
4.081ArgAla: 4.081 ± 0.124
0.897ArgCys: 0.897 ± 0.065
3.655ArgAsp: 3.655 ± 0.128
4.423ArgGlu: 4.423 ± 0.126
2.684ArgPhe: 2.684 ± 0.094
4.045ArgGly: 4.045 ± 0.126
0.913ArgHis: 0.913 ± 0.055
4.901ArgIle: 4.901 ± 0.126
3.924ArgLys: 3.924 ± 0.116
4.497ArgLeu: 4.497 ± 0.135
2.598ArgMet: 2.598 ± 0.092
2.194ArgAsn: 2.194 ± 0.085
2.053ArgPro: 2.053 ± 0.091
1.182ArgGln: 1.182 ± 0.066
3.815ArgArg: 3.815 ± 0.116
3.812ArgSer: 3.812 ± 0.114
3.299ArgThr: 3.299 ± 0.101
3.498ArgVal: 3.498 ± 0.103
0.474ArgTrp: 0.474 ± 0.034
2.181ArgTyr: 2.181 ± 0.086
0.0ArgXaa: 0.0 ± 0.0
Ser
5.471SerAla: 5.471 ± 0.153
0.939SerCys: 0.939 ± 0.064
4.555SerAsp: 4.555 ± 0.139
5.074SerGlu: 5.074 ± 0.124
2.63SerPhe: 2.63 ± 0.089
6.416SerGly: 6.416 ± 0.163
1.028SerHis: 1.028 ± 0.056
4.228SerIle: 4.228 ± 0.119
3.93SerLys: 3.93 ± 0.126
5.698SerLeu: 5.698 ± 0.134
2.303SerMet: 2.303 ± 0.082
1.973SerAsn: 1.973 ± 0.082
2.691SerPro: 2.691 ± 0.09
1.368SerGln: 1.368 ± 0.065
3.725SerArg: 3.725 ± 0.106
4.686SerSer: 4.686 ± 0.147
3.078SerThr: 3.078 ± 0.109
6.015SerVal: 6.015 ± 0.138
0.602SerTrp: 0.602 ± 0.045
1.996SerTyr: 1.996 ± 0.075
0.0SerXaa: 0.0 ± 0.0
Thr
4.449ThrAla: 4.449 ± 0.127
0.727ThrCys: 0.727 ± 0.083
3.398ThrAsp: 3.398 ± 0.104
3.648ThrGlu: 3.648 ± 0.111
1.726ThrPhe: 1.726 ± 0.077
4.939ThrGly: 4.939 ± 0.146
0.83ThrHis: 0.83 ± 0.048
3.437ThrIle: 3.437 ± 0.123
2.457ThrLys: 2.457 ± 0.084
4.334ThrLeu: 4.334 ± 0.117
1.589ThrMet: 1.589 ± 0.08
1.537ThrAsn: 1.537 ± 0.077
2.242ThrPro: 2.242 ± 0.079
0.897ThrGln: 0.897 ± 0.051
2.258ThrArg: 2.258 ± 0.084
3.219ThrSer: 3.219 ± 0.111
2.377ThrThr: 2.377 ± 0.091
4.942ThrVal: 4.942 ± 0.156
0.413ThrTrp: 0.413 ± 0.034
1.768ThrTyr: 1.768 ± 0.089
0.0ThrXaa: 0.0 ± 0.0
Val
5.99ValAla: 5.99 ± 0.158
1.227ValCys: 1.227 ± 0.067
4.289ValAsp: 4.289 ± 0.119
4.52ValGlu: 4.52 ± 0.122
3.113ValPhe: 3.113 ± 0.105
5.464ValGly: 5.464 ± 0.138
1.24ValHis: 1.24 ± 0.065
5.4ValIle: 5.4 ± 0.161
4.17ValLys: 4.17 ± 0.125
7.002ValLeu: 7.002 ± 0.161
2.553ValMet: 2.553 ± 0.095
2.655ValAsn: 2.655 ± 0.074
3.623ValPro: 3.623 ± 0.111
1.448ValGln: 1.448 ± 0.07
4.795ValArg: 4.795 ± 0.128
5.81ValSer: 5.81 ± 0.163
4.417ValThr: 4.417 ± 0.138
5.458ValVal: 5.458 ± 0.163
0.637ValTrp: 0.637 ± 0.044
2.261ValTyr: 2.261 ± 0.083
0.0ValXaa: 0.0 ± 0.0
Trp
0.657TrpAla: 0.657 ± 0.047
0.109TrpCys: 0.109 ± 0.016
0.65TrpAsp: 0.65 ± 0.049
0.551TrpGlu: 0.551 ± 0.041
0.32TrpPhe: 0.32 ± 0.037
0.605TrpGly: 0.605 ± 0.046
0.16TrpHis: 0.16 ± 0.021
0.657TrpIle: 0.657 ± 0.048
0.605TrpLys: 0.605 ± 0.045
0.644TrpLeu: 0.644 ± 0.05
0.372TrpMet: 0.372 ± 0.033
0.439TrpAsn: 0.439 ± 0.039
0.301TrpPro: 0.301 ± 0.03
0.195TrpGln: 0.195 ± 0.021
0.516TrpArg: 0.516 ± 0.037
0.589TrpSer: 0.589 ± 0.05
0.436TrpThr: 0.436 ± 0.031
0.673TrpVal: 0.673 ± 0.047
0.099TrpTrp: 0.099 ± 0.019
0.311TrpTyr: 0.311 ± 0.033
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.438TyrAla: 2.438 ± 0.086
0.519TyrCys: 0.519 ± 0.04
2.37TyrAsp: 2.37 ± 0.093
1.877TyrGlu: 1.877 ± 0.072
1.182TyrPhe: 1.182 ± 0.064
2.899TyrGly: 2.899 ± 0.1
0.554TyrHis: 0.554 ± 0.044
1.947TyrIle: 1.947 ± 0.085
1.329TyrLys: 1.329 ± 0.064
2.723TyrLeu: 2.723 ± 0.106
0.939TyrMet: 0.939 ± 0.062
1.07TyrAsn: 1.07 ± 0.076
1.448TyrPro: 1.448 ± 0.065
0.692TyrGln: 0.692 ± 0.052
1.842TyrArg: 1.842 ± 0.082
2.3TyrSer: 2.3 ± 0.088
1.704TyrThr: 1.704 ± 0.091
2.322TyrVal: 2.322 ± 0.088
0.317TyrTrp: 0.317 ± 0.034
1.015TyrTyr: 1.015 ± 0.056
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1175 proteins (312200 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski