Confluentimicrobium lipolyticum
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3757 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A238JND1|A0A238JND1_9RHOB Uncharacterized protein OS=Confluentimicrobium lipolyticum OX=1524263 GN=COL8621_00576 PE=4 SV=1
MM1 pKa = 7.64 KK2 pKa = 10.06 PLLTLAATAVTALFAQPAAALEE24 pKa = 4.28 LNGGEE29 pKa = 4.22 VQLGYY34 pKa = 9.19 STFPEE39 pKa = 4.18 EE40 pKa = 4.05 TDD42 pKa = 3.4 LSRR45 pKa = 11.84 TALQGSVEE53 pKa = 3.95 IGFTQKK59 pKa = 10.26 FAAQLDD65 pKa = 4.03 LSHH68 pKa = 7.35 FNFDD72 pKa = 3.82 FANEE76 pKa = 4.15 DD77 pKa = 3.64 GQNVTLHH84 pKa = 7.05 AIYY87 pKa = 10.64 NVGPVVSVGAFYY99 pKa = 11.03 GMDD102 pKa = 3.18 KK103 pKa = 11.03 VADD106 pKa = 4.18 ADD108 pKa = 4.03 LDD110 pKa = 4.23 FYY112 pKa = 11.3 GIEE115 pKa = 4.79 GGSQTQVFDD124 pKa = 3.53 TEE126 pKa = 4.92 AYY128 pKa = 10.25 LGYY131 pKa = 10.56 LDD133 pKa = 5.01 GDD135 pKa = 3.64 NDD137 pKa = 3.85 GTLVGLYY144 pKa = 10.65 GRR146 pKa = 11.84 FAVSEE151 pKa = 3.98 NWGLSASYY159 pKa = 10.85 DD160 pKa = 3.57 LADD163 pKa = 3.87 IDD165 pKa = 4.33 GGSDD169 pKa = 3.16 VSRR172 pKa = 11.84 YY173 pKa = 10.5 AFTVDD178 pKa = 2.85 YY179 pKa = 11.17 RR180 pKa = 11.84 MAQGIEE186 pKa = 4.05 LFGQVGSVEE195 pKa = 4.14 IGDD198 pKa = 3.88 DD199 pKa = 3.35 AEE201 pKa = 5.13 AYY203 pKa = 10.28 VGIGAKK209 pKa = 10.04 YY210 pKa = 10.29 RR211 pKa = 11.84 FGADD215 pKa = 2.76 GGVTFGRR222 pKa = 11.84 RR223 pKa = 11.84 GFLEE227 pKa = 4.21 QIPGLL232 pKa = 3.92
Molecular weight: 24.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.884
IPC_protein 3.884
Toseland 3.668
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.579
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.215
Thurlkill 3.719
EMBOSS 3.808
Sillero 3.999
Patrickios 1.901
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.894
Protein with the highest isoelectric point:
>tr|A0A238JLE2|A0A238JLE2_9RHOB Tryptophan synthase beta chain OS=Confluentimicrobium lipolyticum OX=1524263 GN=trpB PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.03 GRR39 pKa = 11.84 KK40 pKa = 8.43 SLCAA44 pKa = 3.75
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.403
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3757
0
3757
1183618
30
2751
315.0
34.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.903 ± 0.052
0.894 ± 0.013
6.093 ± 0.036
6.032 ± 0.039
3.933 ± 0.024
8.509 ± 0.035
2.021 ± 0.019
5.289 ± 0.027
3.64 ± 0.033
9.891 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.763 ± 0.018
2.88 ± 0.023
4.915 ± 0.025
3.209 ± 0.02
6.159 ± 0.036
5.296 ± 0.027
5.575 ± 0.027
7.388 ± 0.033
1.355 ± 0.018
2.255 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here