Streptohalobacillus salinus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Streptohalobacillus

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2307 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V3W2J6|A0A2V3W2J6_9BACI Putative MATE family efflux protein OS=Streptohalobacillus salinus OX=621096 GN=DES38_11625 PE=4 SV=1
MM1 pKa = 7.34KK2 pKa = 10.24KK3 pKa = 10.25VLGVLVTLLLLITLAACGGSEE24 pKa = 5.38DD25 pKa = 4.97EE26 pKa = 5.19DD27 pKa = 4.0TSGDD31 pKa = 3.49NGGEE35 pKa = 4.29GTEE38 pKa = 4.13SDD40 pKa = 4.02GPTEE44 pKa = 4.03VTAWAWDD51 pKa = 3.58PNFNIRR57 pKa = 11.84ALEE60 pKa = 4.06LAEE63 pKa = 3.91EE64 pKa = 4.81AYY66 pKa = 10.58DD67 pKa = 5.15GEE69 pKa = 4.69TEE71 pKa = 4.23LDD73 pKa = 3.69LEE75 pKa = 4.76IIEE78 pKa = 4.59NAQDD82 pKa = 5.44DD83 pKa = 4.02IVQRR87 pKa = 11.84LNTGLSSGTTQGMPNIVLIEE107 pKa = 4.37DD108 pKa = 3.41YY109 pKa = 10.36RR110 pKa = 11.84AQSFLQSYY118 pKa = 9.92PEE120 pKa = 3.98AFYY123 pKa = 10.7PLTDD127 pKa = 4.42YY128 pKa = 11.31INPDD132 pKa = 3.23DD133 pKa = 3.9FASYY137 pKa = 10.27KK138 pKa = 10.49VEE140 pKa = 3.71ATSFDD145 pKa = 3.62GEE147 pKa = 4.45NYY149 pKa = 10.51GLPFDD154 pKa = 4.35SGVTGLYY161 pKa = 10.41VRR163 pKa = 11.84TDD165 pKa = 3.59YY166 pKa = 11.53LEE168 pKa = 4.16EE169 pKa = 5.52AGYY172 pKa = 8.16TVEE175 pKa = 5.62DD176 pKa = 3.72VTDD179 pKa = 3.9ITWSEE184 pKa = 4.13YY185 pKa = 10.38IEE187 pKa = 4.13IGKK190 pKa = 9.29DD191 pKa = 3.17VKK193 pKa = 10.77EE194 pKa = 3.86ATGYY198 pKa = 11.69NMITMDD204 pKa = 4.55PNDD207 pKa = 3.83FGLVRR212 pKa = 11.84MMLQSAGSWYY222 pKa = 10.51FEE224 pKa = 4.46EE225 pKa = 6.13DD226 pKa = 3.17GSTPNLEE233 pKa = 4.17GNPALRR239 pKa = 11.84EE240 pKa = 3.83AFEE243 pKa = 3.97VHH245 pKa = 6.0KK246 pKa = 11.24AMMEE250 pKa = 3.73ADD252 pKa = 3.33IVRR255 pKa = 11.84LHH257 pKa = 6.76SDD259 pKa = 2.93WSQFVGGFNSGDD271 pKa = 3.62VASVPTGNWITPSVKK286 pKa = 10.46AEE288 pKa = 3.74EE289 pKa = 4.31SQAGNWAVVPQPRR302 pKa = 11.84LEE304 pKa = 4.13VDD306 pKa = 3.44GGTTASNLGGSSWYY320 pKa = 10.37VLNTDD325 pKa = 3.43GKK327 pKa = 8.83EE328 pKa = 3.7AAAEE332 pKa = 3.96FLANTFGSNEE342 pKa = 3.83EE343 pKa = 4.47FYY345 pKa = 8.45TTLVEE350 pKa = 4.56EE351 pKa = 4.57IGALGTYY358 pKa = 9.31TPGTQGEE365 pKa = 4.21AFEE368 pKa = 5.78AGDD371 pKa = 4.11EE372 pKa = 4.35FFDD375 pKa = 4.27GQSIIQDD382 pKa = 3.47FSNWTEE388 pKa = 4.02EE389 pKa = 4.15IPPVNYY395 pKa = 10.31GMHH398 pKa = 6.84TYY400 pKa = 10.54AIDD403 pKa = 5.26DD404 pKa = 3.7ILVVEE409 pKa = 4.4MQHH412 pKa = 6.39YY413 pKa = 10.83LDD415 pKa = 4.92GRR417 pKa = 11.84SLDD420 pKa = 3.81EE421 pKa = 4.69ALSNAQDD428 pKa = 3.43QAEE431 pKa = 4.35TQLRR435 pKa = 3.83

Molecular weight: 47.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V3WQH4|A0A2V3WQH4_9BACI Transketolase OS=Streptohalobacillus salinus OX=621096 GN=DES38_10781 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.02KK14 pKa = 8.47VHH16 pKa = 5.57GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTKK25 pKa = 10.2NGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.51KK37 pKa = 10.32GRR39 pKa = 11.84RR40 pKa = 11.84VLSAA44 pKa = 3.76

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2307

0

2307

724221

29

2174

313.9

35.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.377 ± 0.045

0.525 ± 0.012

5.983 ± 0.044

7.387 ± 0.052

4.421 ± 0.039

6.41 ± 0.042

2.323 ± 0.025

7.577 ± 0.049

6.144 ± 0.054

9.844 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.719 ± 0.019

4.166 ± 0.038

3.405 ± 0.027

4.179 ± 0.037

4.191 ± 0.031

5.571 ± 0.037

6.04 ± 0.033

7.275 ± 0.036

0.833 ± 0.018

3.631 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski