Paracoccus mutanolyticus
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2325 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z4PAG3|A0A2Z4PAG3_9RHOB Glyceraldehyde-3-phosphate dehydrogenase OS=Paracoccus mutanolyticus OX=1499308 GN=gap PE=3 SV=1
MM1 pKa = 7.28 TPEE4 pKa = 4.16 NLQEE8 pKa = 4.43 GDD10 pKa = 3.85 PLIAPSTTEE19 pKa = 3.56 HH20 pKa = 7.04 PLYY23 pKa = 10.84 DD24 pKa = 4.08 SVVEE28 pKa = 4.21 ACRR31 pKa = 11.84 TVYY34 pKa = 10.74 DD35 pKa = 3.74 PEE37 pKa = 5.01 IPVNIFDD44 pKa = 5.17 LGLIYY49 pKa = 10.31 TIAIDD54 pKa = 3.18 QANAVRR60 pKa = 11.84 IVMTLTAPGCPVAGEE75 pKa = 4.15 MPGWVADD82 pKa = 3.91 AVGAIPGVGQVDD94 pKa = 3.56 VEE96 pKa = 4.62 MTFQPQWGMDD106 pKa = 3.61 MMSEE110 pKa = 4.19 EE111 pKa = 4.48 ARR113 pKa = 11.84 LEE115 pKa = 4.05 LGFII119 pKa = 4.34
Molecular weight: 12.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.778
IPC2_protein 3.681
IPC_protein 3.605
Toseland 3.414
ProMoST 3.77
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.528
Rodwell 3.452
Grimsley 3.338
Solomon 3.567
Lehninger 3.516
Nozaki 3.732
DTASelect 3.872
Thurlkill 3.478
EMBOSS 3.528
Sillero 3.732
Patrickios 0.693
IPC_peptide 3.567
IPC2_peptide 3.706
IPC2.peptide.svr19 3.71
Protein with the highest isoelectric point:
>tr|A0A2Z4PFZ7|A0A2Z4PFZ7_9RHOB AAA family ATPase OS=Paracoccus mutanolyticus OX=1499308 GN=DPM13_06785 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNLVRR13 pKa = 11.84 ARR15 pKa = 11.84 RR16 pKa = 11.84 HH17 pKa = 4.37 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.12 GGRR29 pKa = 11.84 RR30 pKa = 11.84 VLNARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.53 GRR40 pKa = 11.84 KK41 pKa = 8.73 VLSAA45 pKa = 4.05
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2325
0
2325
548463
25
1156
235.9
25.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.34 ± 0.079
0.999 ± 0.018
5.836 ± 0.041
5.279 ± 0.051
3.334 ± 0.035
9.004 ± 0.05
2.201 ± 0.025
4.691 ± 0.039
2.564 ± 0.041
10.169 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.679 ± 0.026
2.185 ± 0.027
5.874 ± 0.05
3.29 ± 0.024
8.293 ± 0.069
4.808 ± 0.034
4.946 ± 0.038
6.986 ± 0.042
1.55 ± 0.027
1.971 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here