Streptococcus phage Javan220

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AV40|A0A4D6AV40_9CAUD Uncharacterized protein OS=Streptococcus phage Javan220 OX=2548052 GN=Javan220_0035 PE=4 SV=1
MM1 pKa = 7.11TKK3 pKa = 10.56EE4 pKa = 3.99DD5 pKa = 4.31IIYY8 pKa = 10.52IMDD11 pKa = 5.15KK12 pKa = 10.79IMNTALFHH20 pKa = 6.52ANPYY24 pKa = 10.3EE25 pKa = 4.02PFVEE29 pKa = 4.59DD30 pKa = 3.47WEE32 pKa = 4.88SVYY35 pKa = 11.45SLANEE40 pKa = 4.0VCEE43 pKa = 3.97EE44 pKa = 4.21LKK46 pKa = 10.92DD47 pKa = 3.94GKK49 pKa = 10.69

Molecular weight: 5.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AUW7|A0A4D6AUW7_9CAUD Uncharacterized protein OS=Streptococcus phage Javan220 OX=2548052 GN=Javan220_0026 PE=4 SV=1
MM1 pKa = 6.52TTEE4 pKa = 4.98SLIQSKK10 pKa = 10.04IRR12 pKa = 11.84VALSQNGHH20 pKa = 4.93RR21 pKa = 11.84VFRR24 pKa = 11.84ANVGKK29 pKa = 10.59VRR31 pKa = 11.84TADD34 pKa = 3.08GRR36 pKa = 11.84FFDD39 pKa = 3.73TGLPKK44 pKa = 10.75GFSDD48 pKa = 4.67LFGFRR53 pKa = 11.84KK54 pKa = 10.2DD55 pKa = 3.4GQIFFIEE62 pKa = 4.58VKK64 pKa = 10.73NEE66 pKa = 3.82TGRR69 pKa = 11.84VRR71 pKa = 11.84PEE73 pKa = 3.37QKK75 pKa = 10.2QFLEE79 pKa = 4.34TMRR82 pKa = 11.84SFGALAGVARR92 pKa = 11.84SPEE95 pKa = 3.97DD96 pKa = 2.99ALGIVNEE103 pKa = 4.07QRR105 pKa = 11.84IKK107 pKa = 11.06

Molecular weight: 12.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12220

41

1393

218.2

24.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.692 ± 0.581

0.753 ± 0.112

6.391 ± 0.25

7.087 ± 0.434

3.92 ± 0.295

6.596 ± 0.299

1.178 ± 0.121

6.285 ± 0.289

8.396 ± 0.4

8.077 ± 0.247

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.209 ± 0.223

6.056 ± 0.237

2.758 ± 0.187

3.879 ± 0.271

3.764 ± 0.308

6.318 ± 0.451

6.923 ± 0.517

6.399 ± 0.253

1.203 ± 0.103

4.116 ± 0.315
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski