Enterococcus phage EfaCPT1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Efquatrovirus; Enterococcus virus EfaCPT1

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7AUI2|I7AUI2_9CAUD Uncharacterized protein OS=Enterococcus phage EfaCPT1 OX=1204540 GN=EfaCPT1_gp58 PE=4 SV=1
MM1 pKa = 7.4PVKK4 pKa = 9.82TNYY7 pKa = 10.04HH8 pKa = 5.34ICLTNNEE15 pKa = 4.58YY16 pKa = 10.68INLQTEE22 pKa = 4.27TPILVMYY29 pKa = 8.03EE30 pKa = 3.77QAVEE34 pKa = 4.0NDD36 pKa = 3.37EE37 pKa = 5.22KK38 pKa = 11.32LLKK41 pKa = 10.05LDD43 pKa = 3.38KK44 pKa = 11.55AEE46 pKa = 5.25DD47 pKa = 3.61IEE49 pKa = 5.65IDD51 pKa = 4.02GEE53 pKa = 4.27MQPTFITIPLDD64 pKa = 3.76SILYY68 pKa = 9.19VLEE71 pKa = 4.61DD72 pKa = 3.26AA73 pKa = 5.85

Molecular weight: 8.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I7B7B0|I7B7B0_9CAUD Putative major capsid protein OS=Enterococcus phage EfaCPT1 OX=1204540 GN=EfaCPT1_gp05 PE=4 SV=1
MM1 pKa = 7.38GGSRR5 pKa = 11.84KK6 pKa = 10.03LKK8 pKa = 8.76WEE10 pKa = 4.03GVAKK14 pKa = 10.5IFRR17 pKa = 11.84PPLIFLKK24 pKa = 10.78KK25 pKa = 10.63LIGDD29 pKa = 3.92DD30 pKa = 4.1FFSHH34 pKa = 6.75EE35 pKa = 4.03FLKK38 pKa = 10.44TKK40 pKa = 10.05VEE42 pKa = 4.01NMLFPLKK49 pKa = 9.95NTIQNWFLFNCIKK62 pKa = 10.62RR63 pKa = 11.84YY64 pKa = 6.92TEE66 pKa = 4.36CYY68 pKa = 9.43TYY70 pKa = 11.32SLLRR74 pKa = 11.84NTSLITRR81 pKa = 11.84IKK83 pKa = 9.99TGFSNNTFCAIILLGSFFLNLNYY106 pKa = 10.68SKK108 pKa = 11.13GGNYY112 pKa = 9.53YY113 pKa = 10.7SSAKK117 pKa = 9.13TYY119 pKa = 8.32YY120 pKa = 10.32GKK122 pKa = 10.68RR123 pKa = 11.84FAHH126 pKa = 6.04ATNTSVV132 pKa = 3.67

Molecular weight: 15.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

12682

50

1456

183.8

20.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.709 ± 0.417

0.623 ± 0.119

6.127 ± 0.267

8.421 ± 0.564

4.258 ± 0.217

6.411 ± 0.671

1.506 ± 0.161

6.624 ± 0.24

8.8 ± 0.357

8.587 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.178 ± 0.162

6.332 ± 0.294

2.736 ± 0.188

3.864 ± 0.148

3.493 ± 0.178

5.307 ± 0.259

6.119 ± 0.252

6.537 ± 0.269

1.262 ± 0.11

4.108 ± 0.342
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski