Amino acid dipepetide frequency for Enterococcus phage EfaCPT1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.394AlaAla: 0.394 ± 0.183
0.237AlaCys: 0.237 ± 0.163
3.549AlaAsp: 3.549 ± 0.512
3.706AlaGlu: 3.706 ± 0.438
2.366AlaPhe: 2.366 ± 0.577
2.997AlaGly: 2.997 ± 0.471
1.262AlaHis: 1.262 ± 0.345
4.889AlaIle: 4.889 ± 0.823
5.914AlaLys: 5.914 ± 0.708
5.205AlaLeu: 5.205 ± 0.855
2.839AlaMet: 2.839 ± 0.574
3.943AlaAsn: 3.943 ± 0.656
1.735AlaPro: 1.735 ± 0.281
1.656AlaGln: 1.656 ± 0.357
1.656AlaArg: 1.656 ± 0.296
3.47AlaSer: 3.47 ± 0.415
3.943AlaThr: 3.943 ± 0.538
4.101AlaVal: 4.101 ± 0.596
0.71AlaTrp: 0.71 ± 0.25
2.523AlaTyr: 2.523 ± 0.46
0.0AlaXaa: 0.0 ± 0.0
Cys
0.315CysAla: 0.315 ± 0.169
0.0CysCys: 0.0 ± 0.0
0.473CysAsp: 0.473 ± 0.19
0.631CysGlu: 0.631 ± 0.219
0.079CysPhe: 0.079 ± 0.082
0.315CysGly: 0.315 ± 0.168
0.158CysHis: 0.158 ± 0.119
0.631CysIle: 0.631 ± 0.208
0.71CysLys: 0.71 ± 0.248
0.394CysLeu: 0.394 ± 0.16
0.158CysMet: 0.158 ± 0.124
0.552CysAsn: 0.552 ± 0.204
0.0CysPro: 0.0 ± 0.0
0.079CysGln: 0.079 ± 0.079
0.237CysArg: 0.237 ± 0.145
0.394CysSer: 0.394 ± 0.201
0.237CysThr: 0.237 ± 0.148
0.473CysVal: 0.473 ± 0.174
0.237CysTrp: 0.237 ± 0.125
0.158CysTyr: 0.158 ± 0.14
0.0CysXaa: 0.0 ± 0.0
Asp
3.233AspAla: 3.233 ± 0.566
0.315AspCys: 0.315 ± 0.162
2.76AspAsp: 2.76 ± 0.538
4.731AspGlu: 4.731 ± 0.738
2.918AspPhe: 2.918 ± 0.475
4.889AspGly: 4.889 ± 0.654
0.71AspHis: 0.71 ± 0.224
4.574AspIle: 4.574 ± 0.72
5.678AspLys: 5.678 ± 0.677
5.52AspLeu: 5.52 ± 0.654
2.129AspMet: 2.129 ± 0.408
4.574AspAsn: 4.574 ± 0.648
2.05AspPro: 2.05 ± 0.494
1.498AspGln: 1.498 ± 0.259
2.129AspArg: 2.129 ± 0.439
3.706AspSer: 3.706 ± 0.55
2.602AspThr: 2.602 ± 0.543
3.549AspVal: 3.549 ± 0.488
0.71AspTrp: 0.71 ± 0.229
2.997AspTyr: 2.997 ± 0.494
0.0AspXaa: 0.0 ± 0.0
Glu
5.126GluAla: 5.126 ± 0.683
0.552GluCys: 0.552 ± 0.262
3.864GluAsp: 3.864 ± 0.78
8.201GluGlu: 8.201 ± 1.473
3.154GluPhe: 3.154 ± 0.601
5.047GluGly: 5.047 ± 0.62
1.814GluHis: 1.814 ± 0.34
4.101GluIle: 4.101 ± 0.547
5.757GluLys: 5.757 ± 0.63
10.173GluLeu: 10.173 ± 1.109
2.997GluMet: 2.997 ± 0.584
3.549GluAsn: 3.549 ± 0.365
2.445GluPro: 2.445 ± 0.602
4.337GluGln: 4.337 ± 0.572
3.233GluArg: 3.233 ± 0.559
3.627GluSer: 3.627 ± 0.577
4.337GluThr: 4.337 ± 0.525
6.861GluVal: 6.861 ± 0.79
1.498GluTrp: 1.498 ± 0.302
3.549GluTyr: 3.549 ± 0.706
0.0GluXaa: 0.0 ± 0.0
Phe
1.656PheAla: 1.656 ± 0.23
0.237PheCys: 0.237 ± 0.162
3.075PheAsp: 3.075 ± 0.536
3.154PheGlu: 3.154 ± 0.487
1.341PhePhe: 1.341 ± 0.444
2.681PheGly: 2.681 ± 0.551
0.394PheHis: 0.394 ± 0.187
4.022PheIle: 4.022 ± 0.613
4.101PheLys: 4.101 ± 0.617
3.154PheLeu: 3.154 ± 0.548
1.025PheMet: 1.025 ± 0.295
3.312PheAsn: 3.312 ± 0.472
0.71PhePro: 0.71 ± 0.223
1.735PheGln: 1.735 ± 0.386
1.735PheArg: 1.735 ± 0.361
2.366PheSer: 2.366 ± 0.375
3.627PheThr: 3.627 ± 0.6
2.208PheVal: 2.208 ± 0.393
0.473PheTrp: 0.473 ± 0.233
1.498PheTyr: 1.498 ± 0.374
0.0PheXaa: 0.0 ± 0.0
Gly
4.258GlyAla: 4.258 ± 1.079
0.315GlyCys: 0.315 ± 0.161
3.47GlyAsp: 3.47 ± 0.451
3.47GlyGlu: 3.47 ± 0.56
3.706GlyPhe: 3.706 ± 0.493
4.574GlyGly: 4.574 ± 1.34
0.631GlyHis: 0.631 ± 0.22
5.52GlyIle: 5.52 ± 0.932
6.151GlyLys: 6.151 ± 0.804
6.703GlyLeu: 6.703 ± 1.017
1.735GlyMet: 1.735 ± 0.397
3.706GlyAsn: 3.706 ± 0.441
0.71GlyPro: 0.71 ± 0.263
1.577GlyGln: 1.577 ± 0.324
2.523GlyArg: 2.523 ± 0.442
3.864GlySer: 3.864 ± 0.62
3.549GlyThr: 3.549 ± 0.668
4.101GlyVal: 4.101 ± 0.685
0.946GlyTrp: 0.946 ± 0.294
2.602GlyTyr: 2.602 ± 0.501
0.0GlyXaa: 0.0 ± 0.0
His
0.789HisAla: 0.789 ± 0.282
0.237HisCys: 0.237 ± 0.149
0.867HisAsp: 0.867 ± 0.317
0.946HisGlu: 0.946 ± 0.312
1.183HisPhe: 1.183 ± 0.34
1.025HisGly: 1.025 ± 0.34
0.237HisHis: 0.237 ± 0.16
0.631HisIle: 0.631 ± 0.204
1.341HisLys: 1.341 ± 0.337
0.789HisLeu: 0.789 ± 0.259
0.394HisMet: 0.394 ± 0.19
1.183HisAsn: 1.183 ± 0.34
0.315HisPro: 0.315 ± 0.147
0.789HisGln: 0.789 ± 0.264
0.631HisArg: 0.631 ± 0.243
0.552HisSer: 0.552 ± 0.245
0.946HisThr: 0.946 ± 0.385
1.262HisVal: 1.262 ± 0.309
0.079HisTrp: 0.079 ± 0.084
0.867HisTyr: 0.867 ± 0.282
0.0HisXaa: 0.0 ± 0.0
Ile
4.101IleAla: 4.101 ± 0.589
0.552IleCys: 0.552 ± 0.207
5.205IleAsp: 5.205 ± 0.515
7.176IleGlu: 7.176 ± 1.067
2.602IlePhe: 2.602 ± 0.431
3.627IleGly: 3.627 ± 0.517
0.631IleHis: 0.631 ± 0.25
3.864IleIle: 3.864 ± 0.514
7.097IleLys: 7.097 ± 0.656
5.205IleLeu: 5.205 ± 0.656
2.05IleMet: 2.05 ± 0.4
4.179IleAsn: 4.179 ± 0.618
2.366IlePro: 2.366 ± 0.344
2.918IleGln: 2.918 ± 0.477
1.577IleArg: 1.577 ± 0.314
3.391IleSer: 3.391 ± 0.448
4.416IleThr: 4.416 ± 0.481
3.47IleVal: 3.47 ± 0.442
0.552IleTrp: 0.552 ± 0.193
1.262IleTyr: 1.262 ± 0.355
0.0IleXaa: 0.0 ± 0.0
Lys
6.388LysAla: 6.388 ± 0.885
0.473LysCys: 0.473 ± 0.243
5.599LysAsp: 5.599 ± 0.788
9.463LysGlu: 9.463 ± 0.944
2.997LysPhe: 2.997 ± 0.5
4.731LysGly: 4.731 ± 0.788
1.341LysHis: 1.341 ± 0.323
4.022LysIle: 4.022 ± 0.536
5.993LysLys: 5.993 ± 0.748
6.703LysLeu: 6.703 ± 0.778
3.391LysMet: 3.391 ± 0.49
5.283LysAsn: 5.283 ± 0.564
3.312LysPro: 3.312 ± 0.549
4.258LysGln: 4.258 ± 0.562
4.258LysArg: 4.258 ± 0.657
3.943LysSer: 3.943 ± 0.771
5.52LysThr: 5.52 ± 0.645
5.757LysVal: 5.757 ± 0.612
1.104LysTrp: 1.104 ± 0.338
3.47LysTyr: 3.47 ± 0.477
0.0LysXaa: 0.0 ± 0.0
Leu
3.706LeuAla: 3.706 ± 0.521
0.237LeuCys: 0.237 ± 0.177
6.703LeuAsp: 6.703 ± 0.697
8.911LeuGlu: 8.911 ± 1.063
3.627LeuPhe: 3.627 ± 0.506
5.678LeuGly: 5.678 ± 0.956
0.946LeuHis: 0.946 ± 0.259
4.653LeuIle: 4.653 ± 0.887
8.517LeuLys: 8.517 ± 1.055
6.861LeuLeu: 6.861 ± 0.903
2.208LeuMet: 2.208 ± 0.386
5.362LeuAsn: 5.362 ± 0.65
2.523LeuPro: 2.523 ± 0.446
4.179LeuGln: 4.179 ± 0.552
2.208LeuArg: 2.208 ± 0.385
4.968LeuSer: 4.968 ± 0.591
4.416LeuThr: 4.416 ± 0.656
6.151LeuVal: 6.151 ± 0.966
1.183LeuTrp: 1.183 ± 0.273
2.839LeuTyr: 2.839 ± 0.505
0.0LeuXaa: 0.0 ± 0.0
Met
1.893MetAla: 1.893 ± 0.346
0.394MetCys: 0.394 ± 0.184
1.577MetAsp: 1.577 ± 0.35
2.681MetGlu: 2.681 ± 0.61
1.419MetPhe: 1.419 ± 0.423
2.129MetGly: 2.129 ± 0.446
0.158MetHis: 0.158 ± 0.102
2.76MetIle: 2.76 ± 0.457
3.154MetLys: 3.154 ± 0.557
2.366MetLeu: 2.366 ± 0.464
0.473MetMet: 0.473 ± 0.184
1.971MetAsn: 1.971 ± 0.381
0.867MetPro: 0.867 ± 0.255
0.789MetGln: 0.789 ± 0.248
1.814MetArg: 1.814 ± 0.354
1.735MetSer: 1.735 ± 0.44
1.735MetThr: 1.735 ± 0.338
1.735MetVal: 1.735 ± 0.459
0.71MetTrp: 0.71 ± 0.275
1.419MetTyr: 1.419 ± 0.401
0.0MetXaa: 0.0 ± 0.0
Asn
4.101AsnAla: 4.101 ± 0.653
0.473AsnCys: 0.473 ± 0.186
3.154AsnAsp: 3.154 ± 0.541
5.441AsnGlu: 5.441 ± 0.719
2.129AsnPhe: 2.129 ± 0.44
6.23AsnGly: 6.23 ± 0.756
0.71AsnHis: 0.71 ± 0.233
3.549AsnIle: 3.549 ± 0.568
5.599AsnLys: 5.599 ± 0.781
4.731AsnLeu: 4.731 ± 0.613
2.129AsnMet: 2.129 ± 0.349
4.179AsnAsn: 4.179 ± 0.562
1.656AsnPro: 1.656 ± 0.4
1.735AsnGln: 1.735 ± 0.314
1.893AsnArg: 1.893 ± 0.397
2.997AsnSer: 2.997 ± 0.541
4.653AsnThr: 4.653 ± 0.734
3.864AsnVal: 3.864 ± 0.923
1.104AsnTrp: 1.104 ± 0.284
2.997AsnTyr: 2.997 ± 0.526
0.0AsnXaa: 0.0 ± 0.0
Pro
1.814ProAla: 1.814 ± 0.411
0.079ProCys: 0.079 ± 0.076
1.971ProAsp: 1.971 ± 0.405
2.997ProGlu: 2.997 ± 0.553
1.025ProPhe: 1.025 ± 0.272
0.079ProGly: 0.079 ± 0.065
0.394ProHis: 0.394 ± 0.17
1.656ProIle: 1.656 ± 0.33
2.208ProLys: 2.208 ± 0.727
2.839ProLeu: 2.839 ± 0.521
1.183ProMet: 1.183 ± 0.314
1.893ProAsn: 1.893 ± 0.408
0.315ProPro: 0.315 ± 0.177
1.183ProGln: 1.183 ± 0.358
0.867ProArg: 0.867 ± 0.265
1.419ProSer: 1.419 ± 0.287
2.129ProThr: 2.129 ± 0.428
1.656ProVal: 1.656 ± 0.289
0.237ProTrp: 0.237 ± 0.124
1.419ProTyr: 1.419 ± 0.434
0.0ProXaa: 0.0 ± 0.0
Gln
2.208GlnAla: 2.208 ± 0.369
0.315GlnCys: 0.315 ± 0.144
1.498GlnAsp: 1.498 ± 0.342
2.523GlnGlu: 2.523 ± 0.416
1.893GlnPhe: 1.893 ± 0.437
2.05GlnGly: 2.05 ± 0.389
0.552GlnHis: 0.552 ± 0.265
2.918GlnIle: 2.918 ± 0.441
2.287GlnLys: 2.287 ± 0.469
4.258GlnLeu: 4.258 ± 0.484
1.419GlnMet: 1.419 ± 0.288
1.419GlnAsn: 1.419 ± 0.393
1.341GlnPro: 1.341 ± 0.389
2.287GlnGln: 2.287 ± 0.462
1.893GlnArg: 1.893 ± 0.424
2.287GlnSer: 2.287 ± 0.501
2.366GlnThr: 2.366 ± 0.364
2.523GlnVal: 2.523 ± 0.44
0.394GlnTrp: 0.394 ± 0.136
2.208GlnTyr: 2.208 ± 0.35
0.0GlnXaa: 0.0 ± 0.0
Arg
1.893ArgAla: 1.893 ± 0.372
0.473ArgCys: 0.473 ± 0.232
2.445ArgAsp: 2.445 ± 0.447
2.05ArgGlu: 2.05 ± 0.488
2.05ArgPhe: 2.05 ± 0.308
1.814ArgGly: 1.814 ± 0.512
0.71ArgHis: 0.71 ± 0.26
2.445ArgIle: 2.445 ± 0.406
3.075ArgLys: 3.075 ± 0.553
2.918ArgLeu: 2.918 ± 0.581
0.867ArgMet: 0.867 ± 0.241
2.602ArgAsn: 2.602 ± 0.395
1.183ArgPro: 1.183 ± 0.362
1.262ArgGln: 1.262 ± 0.33
1.262ArgArg: 1.262 ± 0.259
1.498ArgSer: 1.498 ± 0.364
2.05ArgThr: 2.05 ± 0.418
2.445ArgVal: 2.445 ± 0.449
0.158ArgTrp: 0.158 ± 0.106
1.735ArgTyr: 1.735 ± 0.408
0.0ArgXaa: 0.0 ± 0.0
Ser
3.312SerAla: 3.312 ± 0.763
0.079SerCys: 0.079 ± 0.074
2.129SerAsp: 2.129 ± 0.337
3.391SerGlu: 3.391 ± 0.557
2.523SerPhe: 2.523 ± 0.389
4.731SerGly: 4.731 ± 0.822
1.893SerHis: 1.893 ± 0.386
3.391SerIle: 3.391 ± 0.446
4.495SerLys: 4.495 ± 0.662
4.337SerLeu: 4.337 ± 0.536
1.577SerMet: 1.577 ± 0.288
3.785SerAsn: 3.785 ± 0.539
0.867SerPro: 0.867 ± 0.254
2.366SerGln: 2.366 ± 0.414
1.341SerArg: 1.341 ± 0.355
3.075SerSer: 3.075 ± 0.693
4.258SerThr: 4.258 ± 0.736
2.523SerVal: 2.523 ± 0.514
0.867SerTrp: 0.867 ± 0.205
2.129SerTyr: 2.129 ± 0.5
0.0SerXaa: 0.0 ± 0.0
Thr
3.706ThrAla: 3.706 ± 0.476
0.158ThrCys: 0.158 ± 0.123
3.549ThrAsp: 3.549 ± 0.538
4.101ThrGlu: 4.101 ± 0.574
2.523ThrPhe: 2.523 ± 0.357
4.574ThrGly: 4.574 ± 0.533
1.104ThrHis: 1.104 ± 0.311
4.574ThrIle: 4.574 ± 0.658
6.782ThrLys: 6.782 ± 0.663
4.81ThrLeu: 4.81 ± 0.765
1.893ThrMet: 1.893 ± 0.396
3.154ThrAsn: 3.154 ± 0.453
2.208ThrPro: 2.208 ± 0.351
2.366ThrGln: 2.366 ± 0.467
2.208ThrArg: 2.208 ± 0.366
2.602ThrSer: 2.602 ± 0.378
4.101ThrThr: 4.101 ± 0.726
4.179ThrVal: 4.179 ± 0.641
0.315ThrTrp: 0.315 ± 0.155
2.287ThrTyr: 2.287 ± 0.581
0.0ThrXaa: 0.0 ± 0.0
Val
5.362ValAla: 5.362 ± 0.716
0.394ValCys: 0.394 ± 0.18
5.441ValAsp: 5.441 ± 0.661
5.52ValGlu: 5.52 ± 0.768
2.287ValPhe: 2.287 ± 0.391
4.022ValGly: 4.022 ± 0.681
0.631ValHis: 0.631 ± 0.224
4.337ValIle: 4.337 ± 0.582
4.731ValLys: 4.731 ± 0.91
4.81ValLeu: 4.81 ± 0.631
2.129ValMet: 2.129 ± 0.33
4.022ValAsn: 4.022 ± 0.653
1.814ValPro: 1.814 ± 0.296
2.129ValGln: 2.129 ± 0.467
1.971ValArg: 1.971 ± 0.337
4.731ValSer: 4.731 ± 0.557
3.075ValThr: 3.075 ± 0.597
4.495ValVal: 4.495 ± 0.63
0.946ValTrp: 0.946 ± 0.292
2.523ValTyr: 2.523 ± 0.486
0.0ValXaa: 0.0 ± 0.0
Trp
0.789TrpAla: 0.789 ± 0.276
0.158TrpCys: 0.158 ± 0.129
0.946TrpAsp: 0.946 ± 0.307
1.498TrpGlu: 1.498 ± 0.357
0.789TrpPhe: 0.789 ± 0.23
0.946TrpGly: 0.946 ± 0.274
0.079TrpHis: 0.079 ± 0.074
0.631TrpIle: 0.631 ± 0.267
0.71TrpLys: 0.71 ± 0.223
1.183TrpLeu: 1.183 ± 0.312
0.158TrpMet: 0.158 ± 0.114
0.789TrpAsn: 0.789 ± 0.335
0.0TrpPro: 0.0 ± 0.0
0.315TrpGln: 0.315 ± 0.13
0.473TrpArg: 0.473 ± 0.223
0.473TrpSer: 0.473 ± 0.159
0.473TrpThr: 0.473 ± 0.156
1.656TrpVal: 1.656 ± 0.399
0.237TrpTrp: 0.237 ± 0.117
0.315TrpTyr: 0.315 ± 0.168
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.05TyrAla: 2.05 ± 0.483
0.552TyrCys: 0.552 ± 0.187
2.997TyrAsp: 2.997 ± 0.635
3.627TyrGlu: 3.627 ± 0.695
1.971TyrPhe: 1.971 ± 0.35
1.971TyrGly: 1.971 ± 0.332
0.71TyrHis: 0.71 ± 0.267
3.075TyrIle: 3.075 ± 0.546
3.706TyrLys: 3.706 ± 0.492
2.918TyrLeu: 2.918 ± 0.511
0.946TyrMet: 0.946 ± 0.274
3.864TyrAsn: 3.864 ± 0.601
0.946TyrPro: 0.946 ± 0.326
1.183TyrGln: 1.183 ± 0.334
1.025TyrArg: 1.025 ± 0.29
1.971TyrSer: 1.971 ± 0.567
2.76TyrThr: 2.76 ± 0.625
2.366TyrVal: 2.366 ± 0.536
0.158TyrTrp: 0.158 ± 0.11
2.287TyrTyr: 2.287 ± 0.548
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 69 proteins (12682 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski