Mycobacterium phage Colbert

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; Pegunavirus; Mycobacterium virus Suffolk

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C9DAE0|C9DAE0_9CAUD Uncharacterized protein OS=Mycobacterium phage Colbert OX=663553 GN=62 PE=4 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84EE4 pKa = 4.11ICQDD8 pKa = 3.48LADD11 pKa = 4.74DD12 pKa = 4.36VFDD15 pKa = 6.55GDD17 pKa = 4.76EE18 pKa = 4.01QWCDD22 pKa = 2.82ITGDD26 pKa = 3.35HH27 pKa = 6.61HH28 pKa = 7.01VCVMTDD34 pKa = 2.66GHH36 pKa = 5.75IARR39 pKa = 11.84RR40 pKa = 11.84THH42 pKa = 5.55EE43 pKa = 4.51CEE45 pKa = 5.85CGITWW50 pKa = 4.11

Molecular weight: 5.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C9DAG5|C9DAG5_9CAUD Uncharacterized protein OS=Mycobacterium phage Colbert OX=663553 GN=87 PE=4 SV=1
MM1 pKa = 7.14TAPEE5 pKa = 4.33KK6 pKa = 10.63ARR8 pKa = 11.84KK9 pKa = 9.32DD10 pKa = 3.37RR11 pKa = 11.84LRR13 pKa = 11.84PVPRR17 pKa = 11.84AGLKK21 pKa = 9.85QIRR24 pKa = 11.84NGEE27 pKa = 3.77ARR29 pKa = 11.84YY30 pKa = 9.85CPSCQTLRR38 pKa = 11.84RR39 pKa = 11.84QTDD42 pKa = 3.05MRR44 pKa = 11.84ITKK47 pKa = 9.97GGVPICIGCRR57 pKa = 11.84DD58 pKa = 3.59NSPRR62 pKa = 11.84VIGG65 pKa = 3.84

Molecular weight: 7.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

21353

36

1992

213.5

22.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.12 ± 0.352

1.105 ± 0.126

6.528 ± 0.261

5.812 ± 0.368

2.281 ± 0.115

9.685 ± 0.622

2.0 ± 0.195

4.594 ± 0.166

2.918 ± 0.145

8.041 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.075 ± 0.098

2.88 ± 0.21

6.093 ± 0.276

3.063 ± 0.127

6.833 ± 0.344

4.945 ± 0.22

7.123 ± 0.21

7.559 ± 0.184

1.84 ± 0.142

2.506 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski