Amino acid dipepetide frequency for Mycobacterium phage Colbert

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.844AlaAla: 17.844 ± 1.47
1.265AlaCys: 1.265 ± 0.261
7.212AlaAsp: 7.212 ± 0.581
7.493AlaGlu: 7.493 ± 0.79
2.389AlaPhe: 2.389 ± 0.371
10.631AlaGly: 10.631 ± 1.271
2.342AlaHis: 2.342 ± 0.326
4.543AlaIle: 4.543 ± 0.56
3.513AlaLys: 3.513 ± 0.457
10.725AlaLeu: 10.725 ± 0.952
3.232AlaMet: 3.232 ± 0.327
2.997AlaAsn: 2.997 ± 0.354
6.65AlaPro: 6.65 ± 0.487
4.075AlaGln: 4.075 ± 0.498
8.805AlaArg: 8.805 ± 0.831
5.62AlaSer: 5.62 ± 0.548
7.915AlaThr: 7.915 ± 0.575
9.086AlaVal: 9.086 ± 0.878
1.967AlaTrp: 1.967 ± 0.295
2.904AlaTyr: 2.904 ± 0.359
0.0AlaXaa: 0.0 ± 0.0
Cys
0.749CysAla: 0.749 ± 0.215
0.0CysCys: 0.0 ± 0.0
0.89CysAsp: 0.89 ± 0.238
0.703CysGlu: 0.703 ± 0.213
0.328CysPhe: 0.328 ± 0.119
1.546CysGly: 1.546 ± 0.296
0.328CysHis: 0.328 ± 0.121
0.281CysIle: 0.281 ± 0.126
0.234CysLys: 0.234 ± 0.117
0.656CysLeu: 0.656 ± 0.214
0.234CysMet: 0.234 ± 0.093
0.328CysAsn: 0.328 ± 0.12
0.89CysPro: 0.89 ± 0.217
0.328CysGln: 0.328 ± 0.112
0.937CysArg: 0.937 ± 0.232
0.468CysSer: 0.468 ± 0.121
0.609CysThr: 0.609 ± 0.181
0.937CysVal: 0.937 ± 0.217
0.281CysTrp: 0.281 ± 0.117
0.328CysTyr: 0.328 ± 0.13
0.0CysXaa: 0.0 ± 0.0
Asp
6.885AspAla: 6.885 ± 0.442
0.937AspCys: 0.937 ± 0.232
4.918AspAsp: 4.918 ± 0.846
5.058AspGlu: 5.058 ± 0.741
1.592AspPhe: 1.592 ± 0.243
6.135AspGly: 6.135 ± 0.66
1.218AspHis: 1.218 ± 0.265
3.091AspIle: 3.091 ± 0.376
1.967AspLys: 1.967 ± 0.347
5.62AspLeu: 5.62 ± 0.431
1.124AspMet: 1.124 ± 0.245
2.201AspAsn: 2.201 ± 0.33
5.386AspPro: 5.386 ± 0.541
1.452AspGln: 1.452 ± 0.285
3.981AspArg: 3.981 ± 0.445
2.951AspSer: 2.951 ± 0.336
3.606AspThr: 3.606 ± 0.518
4.168AspVal: 4.168 ± 0.377
1.171AspTrp: 1.171 ± 0.239
1.827AspTyr: 1.827 ± 0.255
0.0AspXaa: 0.0 ± 0.0
Glu
7.072GluAla: 7.072 ± 0.723
0.656GluCys: 0.656 ± 0.203
3.466GluAsp: 3.466 ± 0.581
2.061GluGlu: 2.061 ± 0.298
2.108GluPhe: 2.108 ± 0.366
4.449GluGly: 4.449 ± 0.51
1.124GluHis: 1.124 ± 0.243
3.84GluIle: 3.84 ± 0.488
1.733GluLys: 1.733 ± 0.259
6.323GluLeu: 6.323 ± 0.611
0.843GluMet: 0.843 ± 0.168
1.077GluAsn: 1.077 ± 0.221
3.325GluPro: 3.325 ± 0.528
2.482GluGln: 2.482 ± 0.305
4.168GluArg: 4.168 ± 0.487
1.592GluSer: 1.592 ± 0.298
3.887GluThr: 3.887 ± 0.432
5.714GluVal: 5.714 ± 0.707
1.124GluTrp: 1.124 ± 0.242
1.077GluTyr: 1.077 ± 0.216
0.0GluXaa: 0.0 ± 0.0
Phe
2.904PheAla: 2.904 ± 0.273
0.234PheCys: 0.234 ± 0.09
1.639PheAsp: 1.639 ± 0.274
1.311PheGlu: 1.311 ± 0.225
0.422PhePhe: 0.422 ± 0.115
2.951PheGly: 2.951 ± 0.405
0.468PheHis: 0.468 ± 0.137
0.609PheIle: 0.609 ± 0.175
1.077PheLys: 1.077 ± 0.222
1.452PheLeu: 1.452 ± 0.281
0.281PheMet: 0.281 ± 0.121
0.843PheAsn: 0.843 ± 0.19
1.311PhePro: 1.311 ± 0.249
0.609PheGln: 0.609 ± 0.17
1.639PheArg: 1.639 ± 0.269
1.171PheSer: 1.171 ± 0.278
1.452PheThr: 1.452 ± 0.229
1.452PheVal: 1.452 ± 0.229
0.328PheTrp: 0.328 ± 0.127
0.656PheTyr: 0.656 ± 0.137
0.0PheXaa: 0.0 ± 0.0
Gly
9.507GlyAla: 9.507 ± 1.42
1.171GlyCys: 1.171 ± 0.272
5.339GlyAsp: 5.339 ± 0.588
5.245GlyGlu: 5.245 ± 0.51
2.061GlyPhe: 2.061 ± 0.309
13.301GlyGly: 13.301 ± 3.029
1.967GlyHis: 1.967 ± 0.362
4.871GlyIle: 4.871 ± 0.389
3.559GlyLys: 3.559 ± 0.484
8.009GlyLeu: 8.009 ± 0.972
2.201GlyMet: 2.201 ± 0.332
2.576GlyAsn: 2.576 ± 0.529
4.496GlyPro: 4.496 ± 0.515
3.419GlyGln: 3.419 ± 0.512
5.948GlyArg: 5.948 ± 0.59
5.199GlySer: 5.199 ± 0.537
6.791GlyThr: 6.791 ± 0.7
6.697GlyVal: 6.697 ± 0.636
1.827GlyTrp: 1.827 ± 0.291
2.67GlyTyr: 2.67 ± 0.353
0.0GlyXaa: 0.0 ± 0.0
His
1.873HisAla: 1.873 ± 0.334
0.281HisCys: 0.281 ± 0.133
1.311HisAsp: 1.311 ± 0.276
1.358HisGlu: 1.358 ± 0.267
0.468HisPhe: 0.468 ± 0.141
1.546HisGly: 1.546 ± 0.269
0.749HisHis: 0.749 ± 0.245
1.452HisIle: 1.452 ± 0.296
0.422HisLys: 0.422 ± 0.159
1.405HisLeu: 1.405 ± 0.316
0.515HisMet: 0.515 ± 0.162
0.515HisAsn: 0.515 ± 0.149
1.171HisPro: 1.171 ± 0.27
0.187HisGln: 0.187 ± 0.09
2.248HisArg: 2.248 ± 0.383
0.843HisSer: 0.843 ± 0.19
1.92HisThr: 1.92 ± 0.375
0.937HisVal: 0.937 ± 0.236
0.187HisTrp: 0.187 ± 0.096
0.609HisTyr: 0.609 ± 0.202
0.0HisXaa: 0.0 ± 0.0
Ile
5.761IleAla: 5.761 ± 0.461
0.609IleCys: 0.609 ± 0.185
3.887IleAsp: 3.887 ± 0.356
4.683IleGlu: 4.683 ± 0.431
0.89IlePhe: 0.89 ± 0.204
5.199IleGly: 5.199 ± 0.573
0.703IleHis: 0.703 ± 0.182
1.546IleIle: 1.546 ± 0.333
1.405IleLys: 1.405 ± 0.305
2.904IleLeu: 2.904 ± 0.345
0.89IleMet: 0.89 ± 0.209
1.358IleAsn: 1.358 ± 0.258
2.716IlePro: 2.716 ± 0.381
0.843IleGln: 0.843 ± 0.221
2.201IleArg: 2.201 ± 0.38
2.435IleSer: 2.435 ± 0.314
3.606IleThr: 3.606 ± 0.366
3.091IleVal: 3.091 ± 0.382
0.328IleTrp: 0.328 ± 0.114
0.89IleTyr: 0.89 ± 0.198
0.0IleXaa: 0.0 ± 0.0
Lys
4.121LysAla: 4.121 ± 0.528
0.234LysCys: 0.234 ± 0.103
1.452LysAsp: 1.452 ± 0.243
0.703LysGlu: 0.703 ± 0.249
0.562LysPhe: 0.562 ± 0.16
2.576LysGly: 2.576 ± 0.338
0.515LysHis: 0.515 ± 0.168
1.592LysIle: 1.592 ± 0.281
1.03LysLys: 1.03 ± 0.295
2.904LysLeu: 2.904 ± 0.372
0.375LysMet: 0.375 ± 0.135
0.749LysAsn: 0.749 ± 0.223
1.358LysPro: 1.358 ± 0.316
1.499LysGln: 1.499 ± 0.285
2.061LysArg: 2.061 ± 0.357
1.405LysSer: 1.405 ± 0.225
2.248LysThr: 2.248 ± 0.331
2.81LysVal: 2.81 ± 0.331
0.328LysTrp: 0.328 ± 0.107
0.656LysTyr: 0.656 ± 0.173
0.0LysXaa: 0.0 ± 0.0
Leu
11.615LeuAla: 11.615 ± 0.885
1.077LeuCys: 1.077 ± 0.255
6.369LeuAsp: 6.369 ± 0.659
3.278LeuGlu: 3.278 ± 0.335
1.873LeuPhe: 1.873 ± 0.367
7.353LeuGly: 7.353 ± 1.138
1.077LeuHis: 1.077 ± 0.194
3.747LeuIle: 3.747 ± 0.517
2.435LeuLys: 2.435 ± 0.393
5.433LeuLeu: 5.433 ± 0.568
1.639LeuMet: 1.639 ± 0.261
3.278LeuAsn: 3.278 ± 0.53
6.042LeuPro: 6.042 ± 0.502
1.592LeuGln: 1.592 ± 0.262
4.028LeuArg: 4.028 ± 0.535
4.168LeuSer: 4.168 ± 0.45
7.447LeuThr: 7.447 ± 0.609
4.871LeuVal: 4.871 ± 0.483
1.265LeuTrp: 1.265 ± 0.24
1.827LeuTyr: 1.827 ± 0.291
0.0LeuXaa: 0.0 ± 0.0
Met
2.435MetAla: 2.435 ± 0.345
0.234MetCys: 0.234 ± 0.111
0.656MetAsp: 0.656 ± 0.154
0.656MetGlu: 0.656 ± 0.137
0.749MetPhe: 0.749 ± 0.19
1.358MetGly: 1.358 ± 0.23
0.422MetHis: 0.422 ± 0.142
0.843MetIle: 0.843 ± 0.174
0.562MetLys: 0.562 ± 0.127
1.499MetLeu: 1.499 ± 0.199
0.375MetMet: 0.375 ± 0.133
0.468MetAsn: 0.468 ± 0.145
1.358MetPro: 1.358 ± 0.258
0.375MetGln: 0.375 ± 0.151
1.546MetArg: 1.546 ± 0.246
1.967MetSer: 1.967 ± 0.325
2.67MetThr: 2.67 ± 0.305
1.452MetVal: 1.452 ± 0.25
0.562MetTrp: 0.562 ± 0.168
0.562MetTyr: 0.562 ± 0.171
0.0MetXaa: 0.0 ± 0.0
Asn
3.419AsnAla: 3.419 ± 0.377
0.234AsnCys: 0.234 ± 0.099
1.967AsnAsp: 1.967 ± 0.362
1.265AsnGlu: 1.265 ± 0.24
0.328AsnPhe: 0.328 ± 0.129
3.466AsnGly: 3.466 ± 0.497
0.562AsnHis: 0.562 ± 0.19
1.358AsnIle: 1.358 ± 0.289
0.937AsnLys: 0.937 ± 0.252
1.92AsnLeu: 1.92 ± 0.282
0.468AsnMet: 0.468 ± 0.128
1.311AsnAsn: 1.311 ± 0.323
1.873AsnPro: 1.873 ± 0.292
0.422AsnGln: 0.422 ± 0.135
2.061AsnArg: 2.061 ± 0.357
1.686AsnSer: 1.686 ± 0.291
2.529AsnThr: 2.529 ± 0.429
1.733AsnVal: 1.733 ± 0.225
0.375AsnTrp: 0.375 ± 0.147
0.89AsnTyr: 0.89 ± 0.21
0.0AsnXaa: 0.0 ± 0.0
Pro
7.681ProAla: 7.681 ± 0.584
0.422ProCys: 0.422 ± 0.121
4.309ProAsp: 4.309 ± 0.596
5.339ProGlu: 5.339 ± 0.707
1.358ProPhe: 1.358 ± 0.245
6.135ProGly: 6.135 ± 0.794
1.452ProHis: 1.452 ± 0.315
2.623ProIle: 2.623 ± 0.418
1.171ProLys: 1.171 ± 0.223
3.559ProLeu: 3.559 ± 0.383
1.218ProMet: 1.218 ± 0.232
1.967ProAsn: 1.967 ± 0.257
4.59ProPro: 4.59 ± 0.655
1.733ProGln: 1.733 ± 0.232
3.747ProArg: 3.747 ± 0.529
2.435ProSer: 2.435 ± 0.377
3.794ProThr: 3.794 ± 0.369
5.011ProVal: 5.011 ± 0.475
1.311ProTrp: 1.311 ± 0.234
1.077ProTyr: 1.077 ± 0.241
0.0ProXaa: 0.0 ± 0.0
Gln
4.215GlnAla: 4.215 ± 0.756
0.281GlnCys: 0.281 ± 0.117
1.03GlnAsp: 1.03 ± 0.237
0.843GlnGlu: 0.843 ± 0.19
1.03GlnPhe: 1.03 ± 0.222
2.201GlnGly: 2.201 ± 0.354
0.937GlnHis: 0.937 ± 0.186
2.061GlnIle: 2.061 ± 0.298
0.796GlnLys: 0.796 ± 0.244
2.389GlnLeu: 2.389 ± 0.35
0.609GlnMet: 0.609 ± 0.151
0.703GlnAsn: 0.703 ± 0.21
1.452GlnPro: 1.452 ± 0.244
1.546GlnGln: 1.546 ± 0.245
2.904GlnArg: 2.904 ± 0.42
1.78GlnSer: 1.78 ± 0.338
2.389GlnThr: 2.389 ± 0.35
2.061GlnVal: 2.061 ± 0.322
0.703GlnTrp: 0.703 ± 0.18
0.703GlnTyr: 0.703 ± 0.207
0.0GlnXaa: 0.0 ± 0.0
Arg
7.447ArgAla: 7.447 ± 0.715
0.609ArgCys: 0.609 ± 0.148
4.356ArgAsp: 4.356 ± 0.465
5.011ArgGlu: 5.011 ± 0.646
1.546ArgPhe: 1.546 ± 0.258
4.121ArgGly: 4.121 ± 0.539
1.733ArgHis: 1.733 ± 0.314
2.482ArgIle: 2.482 ± 0.281
1.78ArgLys: 1.78 ± 0.323
5.995ArgLeu: 5.995 ± 0.473
1.358ArgMet: 1.358 ± 0.298
2.342ArgAsn: 2.342 ± 0.322
4.075ArgPro: 4.075 ± 0.543
2.576ArgGln: 2.576 ± 0.301
6.557ArgArg: 6.557 ± 0.75
2.763ArgSer: 2.763 ± 0.291
4.028ArgThr: 4.028 ± 0.447
5.433ArgVal: 5.433 ± 0.712
1.78ArgTrp: 1.78 ± 0.296
2.295ArgTyr: 2.295 ± 0.393
0.0ArgXaa: 0.0 ± 0.0
Ser
5.058SerAla: 5.058 ± 0.574
0.562SerCys: 0.562 ± 0.145
3.232SerAsp: 3.232 ± 0.293
2.108SerGlu: 2.108 ± 0.345
0.703SerPhe: 0.703 ± 0.195
5.948SerGly: 5.948 ± 0.72
0.703SerHis: 0.703 ± 0.184
1.639SerIle: 1.639 ± 0.297
1.265SerLys: 1.265 ± 0.234
4.168SerLeu: 4.168 ± 0.676
1.452SerMet: 1.452 ± 0.272
1.124SerAsn: 1.124 ± 0.259
3.138SerPro: 3.138 ± 0.408
2.014SerGln: 2.014 ± 0.286
3.325SerArg: 3.325 ± 0.272
2.482SerSer: 2.482 ± 0.453
3.887SerThr: 3.887 ± 0.541
4.075SerVal: 4.075 ± 0.463
1.03SerTrp: 1.03 ± 0.224
1.546SerTyr: 1.546 ± 0.253
0.0SerXaa: 0.0 ± 0.0
Thr
8.617ThrAla: 8.617 ± 0.597
0.562ThrCys: 0.562 ± 0.149
5.386ThrAsp: 5.386 ± 0.575
3.466ThrGlu: 3.466 ± 0.413
1.592ThrPhe: 1.592 ± 0.266
7.025ThrGly: 7.025 ± 0.587
1.124ThrHis: 1.124 ± 0.238
3.887ThrIle: 3.887 ± 0.472
2.435ThrLys: 2.435 ± 0.305
5.339ThrLeu: 5.339 ± 0.533
1.592ThrMet: 1.592 ± 0.26
1.827ThrAsn: 1.827 ± 0.367
5.245ThrPro: 5.245 ± 0.643
1.92ThrGln: 1.92 ± 0.316
3.84ThrArg: 3.84 ± 0.41
4.168ThrSer: 4.168 ± 0.391
4.121ThrThr: 4.121 ± 0.501
6.182ThrVal: 6.182 ± 0.527
1.311ThrTrp: 1.311 ± 0.23
1.592ThrTyr: 1.592 ± 0.273
0.0ThrXaa: 0.0 ± 0.0
Val
8.383ValAla: 8.383 ± 0.452
0.937ValCys: 0.937 ± 0.234
4.356ValAsp: 4.356 ± 0.419
5.526ValGlu: 5.526 ± 0.644
1.452ValPhe: 1.452 ± 0.209
7.634ValGly: 7.634 ± 0.613
1.639ValHis: 1.639 ± 0.251
3.513ValIle: 3.513 ± 0.328
1.967ValLys: 1.967 ± 0.334
6.557ValLeu: 6.557 ± 0.462
1.499ValMet: 1.499 ± 0.31
2.154ValAsn: 2.154 ± 0.257
3.84ValPro: 3.84 ± 0.331
1.873ValGln: 1.873 ± 0.244
4.824ValArg: 4.824 ± 0.483
4.121ValSer: 4.121 ± 0.381
5.339ValThr: 5.339 ± 0.478
6.135ValVal: 6.135 ± 0.716
1.92ValTrp: 1.92 ± 0.309
1.92ValTyr: 1.92 ± 0.44
0.0ValXaa: 0.0 ± 0.0
Trp
2.342TrpAla: 2.342 ± 0.415
0.422TrpCys: 0.422 ± 0.139
1.358TrpAsp: 1.358 ± 0.246
0.515TrpGlu: 0.515 ± 0.153
0.749TrpPhe: 0.749 ± 0.258
1.03TrpGly: 1.03 ± 0.222
0.468TrpHis: 0.468 ± 0.159
0.89TrpIle: 0.89 ± 0.199
0.281TrpLys: 0.281 ± 0.111
1.592TrpLeu: 1.592 ± 0.325
0.468TrpMet: 0.468 ± 0.149
0.281TrpAsn: 0.281 ± 0.089
0.89TrpPro: 0.89 ± 0.234
0.749TrpGln: 0.749 ± 0.218
1.311TrpArg: 1.311 ± 0.224
1.265TrpSer: 1.265 ± 0.239
1.405TrpThr: 1.405 ± 0.261
1.546TrpVal: 1.546 ± 0.363
0.422TrpTrp: 0.422 ± 0.148
0.422TrpTyr: 0.422 ± 0.141
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.278TyrAla: 3.278 ± 0.303
0.328TyrCys: 0.328 ± 0.122
2.154TyrAsp: 2.154 ± 0.287
1.499TyrGlu: 1.499 ± 0.285
0.609TyrPhe: 0.609 ± 0.18
2.248TyrGly: 2.248 ± 0.389
0.468TyrHis: 0.468 ± 0.144
1.077TyrIle: 1.077 ± 0.215
0.609TyrLys: 0.609 ± 0.162
1.967TyrLeu: 1.967 ± 0.376
0.328TyrMet: 0.328 ± 0.144
0.703TyrAsn: 0.703 ± 0.145
1.124TyrPro: 1.124 ± 0.267
0.937TyrGln: 0.937 ± 0.215
2.201TyrArg: 2.201 ± 0.352
0.937TyrSer: 0.937 ± 0.178
1.592TyrThr: 1.592 ± 0.281
2.201TyrVal: 2.201 ± 0.287
0.187TyrTrp: 0.187 ± 0.091
0.609TyrTyr: 0.609 ± 0.168
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 100 proteins (21353 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski