Leptomonas pyrrhocoris

Taxonomy: cellular organisms; Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina; Trypanosomatida; Trypanosomatidae; Leishmaniinae; Leptomonas

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9272 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M9G527|A0A0M9G527_9TRYP Asparagine--tRNA ligase OS=Leptomonas pyrrhocoris OX=157538 GN=ABB37_03391 PE=3 SV=1
MM1 pKa = 7.69TDD3 pKa = 2.85VTSQQGSASDD13 pKa = 3.5VVTAVQQVRR22 pKa = 11.84DD23 pKa = 3.95DD24 pKa = 3.9YY25 pKa = 11.24MKK27 pKa = 10.72FFSDD31 pKa = 3.52VFTDD35 pKa = 3.8EE36 pKa = 5.01LVEE39 pKa = 4.3LYY41 pKa = 10.41EE42 pKa = 4.31VDD44 pKa = 3.55GSKK47 pKa = 11.05DD48 pKa = 3.25AVKK51 pKa = 10.55QLTACIEE58 pKa = 4.3SGVAVYY64 pKa = 7.97GHH66 pKa = 7.6PIYY69 pKa = 11.14VDD71 pKa = 3.98DD72 pKa = 4.41PHH74 pKa = 8.18LSSSS78 pKa = 3.38

Molecular weight: 8.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M9G022|A0A0M9G022_9TRYP Uncharacterized protein OS=Leptomonas pyrrhocoris OX=157538 GN=ABB37_05246 PE=4 SV=1
MM1 pKa = 6.91GTVSRR6 pKa = 11.84PRR8 pKa = 11.84GMRR11 pKa = 11.84PKK13 pKa = 8.85WHH15 pKa = 6.99KK16 pKa = 10.7KK17 pKa = 8.61RR18 pKa = 11.84IKK20 pKa = 9.87RR21 pKa = 11.84LKK23 pKa = 9.0LRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84MRR30 pKa = 11.84QRR32 pKa = 11.84SKK34 pKa = 11.39

Molecular weight: 4.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9272

0

9272

5711352

34

7638

616.0

66.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.842 ± 0.034

1.643 ± 0.01

5.011 ± 0.016

6.042 ± 0.025

3.107 ± 0.016

6.454 ± 0.021

2.649 ± 0.01

2.841 ± 0.015

3.413 ± 0.022

8.896 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.134 ± 0.01

3.008 ± 0.013

6.047 ± 0.031

4.113 ± 0.017

7.097 ± 0.02

8.762 ± 0.035

6.348 ± 0.021

7.164 ± 0.02

1.041 ± 0.007

2.378 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski