Polaribacter atrinae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Polaribacter

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3258 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A176TEQ9|A0A176TEQ9_9FLAO Methionine--tRNA ligase OS=Polaribacter atrinae OX=1333662 GN=metG PE=3 SV=1
MM1 pKa = 7.71LFFCFITFQNINAQLSKK18 pKa = 10.91KK19 pKa = 10.07HH20 pKa = 6.51FIPPLTSTDD29 pKa = 3.33GFTDD33 pKa = 3.12QYY35 pKa = 11.33IYY37 pKa = 10.74ISTPNNNVSYY47 pKa = 10.72KK48 pKa = 8.61ITPVGNLDD56 pKa = 3.17QTAYY60 pKa = 10.84SGIVSNNLPIEE71 pKa = 4.1QPVLNLSGNTDD82 pKa = 4.07FDD84 pKa = 4.44NDD86 pKa = 3.96TQLHH90 pKa = 5.86IPEE93 pKa = 4.59SSIIGNKK100 pKa = 6.03FTNRR104 pKa = 11.84GFIIEE109 pKa = 4.62ANDD112 pKa = 4.46LIYY115 pKa = 10.27VTIRR119 pKa = 11.84VRR121 pKa = 11.84SSQANNGNKK130 pKa = 8.8NQAGALVSKK139 pKa = 10.59GNSALGTEE147 pKa = 4.37FRR149 pKa = 11.84IGGFIRR155 pKa = 11.84DD156 pKa = 3.58GSINGDD162 pKa = 3.46VTFASIMATEE172 pKa = 5.18DD173 pKa = 3.29NTEE176 pKa = 3.97VTITNATSSSTIININLNEE195 pKa = 4.25GEE197 pKa = 4.53SYY199 pKa = 10.43IIHH202 pKa = 7.07ANGANSSSIIGNLITSDD219 pKa = 3.32KK220 pKa = 10.25PIVVNSGSAYY230 pKa = 10.37GSFGNNSGNADD241 pKa = 3.59YY242 pKa = 11.23GFDD245 pKa = 3.84QIVGADD251 pKa = 3.93KK252 pKa = 10.78IGSEE256 pKa = 4.45YY257 pKa = 10.6ILVKK261 pKa = 10.86GSGNTIEE268 pKa = 4.38KK269 pKa = 10.46SLIIAHH275 pKa = 6.68EE276 pKa = 4.48DD277 pKa = 3.14FTEE280 pKa = 4.41FFINDD285 pKa = 3.49TSVGTFNAGEE295 pKa = 4.18YY296 pKa = 10.66YY297 pKa = 9.24ITTNSMYY304 pKa = 7.63TTNGNMYY311 pKa = 10.19INTNKK316 pKa = 9.96PVFVYY321 pKa = 10.32QGVGGLGNNGIPSEE335 pKa = 4.16ANQGLFFVPPLSCEE349 pKa = 3.84NRR351 pKa = 11.84GKK353 pKa = 10.63VDD355 pKa = 4.67NIPHH359 pKa = 6.73IEE361 pKa = 4.25RR362 pKa = 11.84IGNEE366 pKa = 4.18DD367 pKa = 3.26FTGGISIVTNEE378 pKa = 4.17DD379 pKa = 3.44SVVKK383 pKa = 10.67INGSLITTFSGISGPFLITTKK404 pKa = 10.93DD405 pKa = 2.99SGKK408 pKa = 9.67YY409 pKa = 8.48DD410 pKa = 3.58TYY412 pKa = 11.42KK413 pKa = 9.78VTGLEE418 pKa = 4.18GNISIEE424 pKa = 4.2SSGEE428 pKa = 3.83LYY430 pKa = 10.5CAYY433 pKa = 10.46FNQNGLATSGAFYY446 pKa = 10.75SGFPSSPEE454 pKa = 3.33IKK456 pKa = 10.37FNVAISTLGNCLGNDD471 pKa = 4.52LEE473 pKa = 4.44LQAANTEE480 pKa = 4.39LFDD483 pKa = 3.9SFKK486 pKa = 10.57WYY488 pKa = 10.7FNDD491 pKa = 3.59GSGYY495 pKa = 9.83IDD497 pKa = 3.66TNEE500 pKa = 3.95TSATLIPSAPGSYY513 pKa = 10.4QLIGKK518 pKa = 8.73INCTNTTFKK527 pKa = 10.2STEE530 pKa = 4.0IPVSICPDD538 pKa = 3.88DD539 pKa = 3.97YY540 pKa = 11.91DD541 pKa = 5.05KK542 pKa = 11.78DD543 pKa = 4.35GIIDD547 pKa = 4.51NIDD550 pKa = 2.88VDD552 pKa = 3.81IDD554 pKa = 3.54NDD556 pKa = 4.61GILNIDD562 pKa = 3.73EE563 pKa = 4.43SLGDD567 pKa = 4.13KK568 pKa = 10.54IIDD571 pKa = 3.85LSDD574 pKa = 3.29INNPTVEE581 pKa = 4.86DD582 pKa = 4.25DD583 pKa = 3.55VTTIITNTFTGDD595 pKa = 2.82ATGNFTSVANNVTTSNATYY614 pKa = 10.92SLDD617 pKa = 3.64FNDD620 pKa = 4.86NINFKK625 pKa = 9.2LTQNRR630 pKa = 11.84TTDD633 pKa = 3.22HH634 pKa = 6.54TISNNEE640 pKa = 3.51YY641 pKa = 10.53FIIKK645 pKa = 10.46LSQTEE650 pKa = 4.13KK651 pKa = 10.94NITLIDD657 pKa = 4.36PDD659 pKa = 3.96NQILIDD665 pKa = 4.18SNFDD669 pKa = 5.1DD670 pKa = 4.23EE671 pKa = 5.88FEE673 pKa = 5.6DD674 pKa = 4.7NFTQFSASVIKK685 pKa = 10.49FKK687 pKa = 11.11FKK689 pKa = 11.04ADD691 pKa = 3.78LVGSTSTFQFVANQINNISFEE712 pKa = 4.28HH713 pKa = 6.49YY714 pKa = 11.01NDD716 pKa = 3.24ISSNASTFNGNIGLTCFSLDD736 pKa = 3.26SDD738 pKa = 4.01GDD740 pKa = 4.14GIEE743 pKa = 5.74DD744 pKa = 4.25MFDD747 pKa = 4.28LDD749 pKa = 4.25TDD751 pKa = 3.61NDD753 pKa = 5.31GIPDD757 pKa = 4.28LYY759 pKa = 10.87DD760 pKa = 3.89AYY762 pKa = 10.64GQEE765 pKa = 3.76ISLAKK770 pKa = 9.87TDD772 pKa = 4.09ANLDD776 pKa = 3.52GLDD779 pKa = 4.41DD780 pKa = 3.94VFEE783 pKa = 5.33SITTNLDD790 pKa = 3.03TDD792 pKa = 3.88GDD794 pKa = 4.35GVNNYY799 pKa = 9.9LDD801 pKa = 4.0YY802 pKa = 11.49DD803 pKa = 3.92SDD805 pKa = 3.92NDD807 pKa = 4.57GIYY810 pKa = 10.82DD811 pKa = 3.66STEE814 pKa = 3.73ASHH817 pKa = 7.07GLDD820 pKa = 2.95TDD822 pKa = 4.07FNGTIDD828 pKa = 3.64TFIDD832 pKa = 3.22INKK835 pKa = 10.02NGLEE839 pKa = 4.24DD840 pKa = 3.7SLEE843 pKa = 4.22NDD845 pKa = 3.56TTVQTISLKK854 pKa = 9.4YY855 pKa = 9.17TIADD859 pKa = 3.57TDD861 pKa = 3.37ADD863 pKa = 4.26AIFNFIEE870 pKa = 4.69LDD872 pKa = 3.63SDD874 pKa = 4.25ADD876 pKa = 3.67GCNDD880 pKa = 3.29VIEE883 pKa = 5.78AGFTDD888 pKa = 3.86NNNDD892 pKa = 3.73GILFANSFSVDD903 pKa = 3.15EE904 pKa = 4.39NGKK907 pKa = 9.76VKK909 pKa = 10.76NPNDD913 pKa = 4.15GYY915 pKa = 7.67TTPNSNYY922 pKa = 6.83TTSAPIVVTTFTDD935 pKa = 3.44VTFCEE940 pKa = 4.22NLTDD944 pKa = 5.71IITIDD949 pKa = 3.56SNADD953 pKa = 3.06SFQWEE958 pKa = 4.49FSTDD962 pKa = 3.07GTNWLIINEE971 pKa = 4.62DD972 pKa = 3.93DD973 pKa = 4.24TTDD976 pKa = 3.89LYY978 pKa = 11.25QGSTSASLQITNTPFTYY995 pKa = 10.23DD996 pKa = 2.83SYY998 pKa = 11.32QYY1000 pKa = 10.66RR1001 pKa = 11.84VALNRR1006 pKa = 11.84TGNSCGFTSDD1016 pKa = 5.5AITLTVNPKK1025 pKa = 9.91PVIEE1029 pKa = 4.54NNPAALFQCDD1039 pKa = 4.04TDD1041 pKa = 4.67SDD1043 pKa = 4.14LQTTFNLTEE1052 pKa = 4.17SEE1054 pKa = 3.84ISITNEE1060 pKa = 3.12TDD1062 pKa = 3.32VNFEE1066 pKa = 4.23YY1067 pKa = 11.09YY1068 pKa = 9.36ATEE1071 pKa = 3.93LDD1073 pKa = 4.88AINGTPQVADD1083 pKa = 3.37KK1084 pKa = 10.72TSYY1087 pKa = 10.81SVNTNGEE1094 pKa = 4.05AWVKK1098 pKa = 9.78TISNTTACYY1107 pKa = 10.27SISKK1111 pKa = 10.27INLTVSYY1118 pKa = 10.76APNEE1122 pKa = 4.34TFNDD1126 pKa = 3.41TLYY1129 pKa = 10.97QCDD1132 pKa = 4.82DD1133 pKa = 4.27LLDD1136 pKa = 4.59LDD1138 pKa = 5.57GNNTTNNDD1146 pKa = 3.72DD1147 pKa = 3.69KK1148 pKa = 11.81DD1149 pKa = 4.54GITSFDD1155 pKa = 3.94LSNISDD1161 pKa = 4.48KK1162 pKa = 11.02ISTDD1166 pKa = 2.96SDD1168 pKa = 3.28IKK1170 pKa = 10.82IEE1172 pKa = 4.72FYY1174 pKa = 10.16EE1175 pKa = 4.22TDD1177 pKa = 3.48DD1178 pKa = 4.74DD1179 pKa = 4.03RR1180 pKa = 11.84TRR1182 pKa = 11.84SLNEE1186 pKa = 3.26ITEE1189 pKa = 4.42TQDD1192 pKa = 3.08LASYY1196 pKa = 10.37RR1197 pKa = 11.84NKK1199 pKa = 10.51NYY1201 pKa = 10.09PFTTGNPVTIFYY1213 pKa = 10.91KK1214 pKa = 10.8LISITNNNCQGIGEE1228 pKa = 4.97FYY1230 pKa = 11.2LEE1232 pKa = 4.24VNKK1235 pKa = 10.29IPEE1238 pKa = 4.06FTVDD1242 pKa = 4.24GEE1244 pKa = 4.44APSDD1248 pKa = 4.22PIIICAEE1255 pKa = 4.21NIPYY1259 pKa = 8.67TLNVYY1264 pKa = 8.93TSNSSYY1270 pKa = 11.06NYY1272 pKa = 9.35QWTNEE1277 pKa = 4.03KK1278 pKa = 10.56GDD1280 pKa = 3.97NLGTTQNINISDD1292 pKa = 3.67AGVYY1296 pKa = 8.72TVTSSTNSCSRR1307 pKa = 11.84SRR1309 pKa = 11.84TITVYY1314 pKa = 10.79KK1315 pKa = 10.5SDD1317 pKa = 3.87FEE1319 pKa = 4.22NLEE1322 pKa = 4.03EE1323 pKa = 4.45SFVTITDD1330 pKa = 3.69DD1331 pKa = 4.61LSVINSNLNIRR1342 pKa = 11.84IDD1344 pKa = 3.45IPTNPLINEE1353 pKa = 4.3VFQYY1357 pKa = 11.39ALEE1360 pKa = 5.29DD1361 pKa = 3.81EE1362 pKa = 5.24DD1363 pKa = 5.17GMTIRR1368 pKa = 11.84SFQDD1372 pKa = 3.03SNVFEE1377 pKa = 5.49DD1378 pKa = 3.98IEE1380 pKa = 4.41GGIYY1384 pKa = 9.98KK1385 pKa = 10.17IIVEE1389 pKa = 4.48SKK1391 pKa = 9.03NGCGTSEE1398 pKa = 4.47LLVSVIQFPKK1408 pKa = 10.42FFTPNGDD1415 pKa = 3.34RR1416 pKa = 11.84KK1417 pKa = 10.33NDD1419 pKa = 2.79SWGIKK1424 pKa = 9.47GVSNTLYY1431 pKa = 10.49QSNSSINIFNRR1442 pKa = 11.84YY1443 pKa = 7.94GKK1445 pKa = 10.34LVAQTTIDD1453 pKa = 3.51GDD1455 pKa = 3.88GWDD1458 pKa = 3.44GTYY1461 pKa = 10.52NGKK1464 pKa = 9.2LLPSDD1469 pKa = 4.78DD1470 pKa = 3.67YY1471 pKa = 10.8WYY1473 pKa = 10.48SIQLIPVNTDD1483 pKa = 2.8QKK1485 pKa = 10.67PILKK1489 pKa = 9.71KK1490 pKa = 10.6GHH1492 pKa = 6.37FSLIRR1497 pKa = 11.84KK1498 pKa = 8.22

Molecular weight:
164.3 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A176TAX6|A0A176TAX6_9FLAO Glutamate-1-semialdehyde 2 1-aminomutase OS=Polaribacter atrinae OX=1333662 GN=hemL PE=3 SV=1
MM1 pKa = 7.51ARR3 pKa = 11.84SLKK6 pKa = 10.37LWLSNTEE13 pKa = 3.61AEE15 pKa = 4.24KK16 pKa = 10.87YY17 pKa = 10.09IRR19 pKa = 11.84VSKK22 pKa = 8.47TLKK25 pKa = 9.92PYY27 pKa = 10.31IMKK30 pKa = 10.36KK31 pKa = 10.36GVLILIGMFFLVSTIKK47 pKa = 10.91AEE49 pKa = 4.21NNKK52 pKa = 9.29EE53 pKa = 4.1VPNKK57 pKa = 10.13IGFNYY62 pKa = 10.23SYY64 pKa = 10.62EE65 pKa = 4.08NTVNFVEE72 pKa = 4.4RR73 pKa = 11.84DD74 pKa = 3.33VEE76 pKa = 4.92FFIFTNGEE84 pKa = 3.72FDD86 pKa = 4.53FNTHH90 pKa = 4.44YY91 pKa = 10.05TSNRR95 pKa = 11.84NEE97 pKa = 3.59IGVFISRR104 pKa = 11.84DD105 pKa = 3.17YY106 pKa = 10.57RR107 pKa = 11.84GRR109 pKa = 11.84IEE111 pKa = 5.14RR112 pKa = 11.84IGNSFINYY120 pKa = 8.99DD121 pKa = 3.08RR122 pKa = 11.84YY123 pKa = 11.52GNVTRR128 pKa = 11.84IGNVFMKK135 pKa = 9.87YY136 pKa = 9.52YY137 pKa = 10.35RR138 pKa = 11.84GQLTDD143 pKa = 3.25VGNLKK148 pKa = 9.66IRR150 pKa = 11.84YY151 pKa = 7.53DD152 pKa = 2.91HH153 pKa = 6.7WGNPKK158 pKa = 10.17FYY160 pKa = 11.29GNVKK164 pKa = 10.41DD165 pKa = 5.26NYY167 pKa = 8.65YY168 pKa = 10.23HH169 pKa = 6.91YY170 pKa = 11.24NGLRR174 pKa = 11.84INLNIGNIFEE184 pKa = 4.66YY185 pKa = 10.38NNSYY189 pKa = 10.36FSHH192 pKa = 7.28RR193 pKa = 11.84DD194 pKa = 3.41FARR197 pKa = 11.84HH198 pKa = 4.36YY199 pKa = 11.1SKK201 pKa = 10.38IRR203 pKa = 11.84EE204 pKa = 4.14DD205 pKa = 3.2NKK207 pKa = 10.1YY208 pKa = 10.49YY209 pKa = 10.58YY210 pKa = 11.05YY211 pKa = 10.92KK212 pKa = 10.81ANPNAKK218 pKa = 9.29IGNKK222 pKa = 9.81SKK224 pKa = 10.43ILRR227 pKa = 11.84RR228 pKa = 11.84RR229 pKa = 11.84KK230 pKa = 8.52PAPVTRR236 pKa = 11.84RR237 pKa = 11.84SNTTYY242 pKa = 10.61RR243 pKa = 11.84KK244 pKa = 9.41PVTINSNRR252 pKa = 11.84RR253 pKa = 11.84TVVGKK258 pKa = 10.49RR259 pKa = 11.84NNSTTRR265 pKa = 11.84RR266 pKa = 11.84STTINTNKK274 pKa = 10.27NGTIDD279 pKa = 3.38RR280 pKa = 11.84RR281 pKa = 11.84NNSTVTRR288 pKa = 11.84RR289 pKa = 11.84PTTVTRR295 pKa = 11.84GKK297 pKa = 6.52TTSVKK302 pKa = 10.76RR303 pKa = 11.84NDD305 pKa = 3.4NNSNRR310 pKa = 11.84KK311 pKa = 6.39PTVVKK316 pKa = 10.53RR317 pKa = 11.84KK318 pKa = 9.14NEE320 pKa = 4.05KK321 pKa = 10.21KK322 pKa = 10.29KK323 pKa = 10.27ATTKK327 pKa = 10.58EE328 pKa = 3.67EE329 pKa = 4.09NNRR332 pKa = 11.84KK333 pKa = 9.23RR334 pKa = 11.84RR335 pKa = 11.84SS336 pKa = 3.28

Molecular weight:
39.8 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3258

0

3258

1114503

50

2354

342.1

38.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.044 ± 0.039

0.666 ± 0.012

5.535 ± 0.034

6.685 ± 0.036

5.43 ± 0.036

6.143 ± 0.043

1.641 ± 0.018

8.348 ± 0.043

8.665 ± 0.055

9.183 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.002 ± 0.021

6.592 ± 0.045

3.081 ± 0.021

3.121 ± 0.023

3.072 ± 0.027

6.595 ± 0.035

5.998 ± 0.04

6.156 ± 0.031

1.02 ± 0.015

4.025 ± 0.025

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski