Polaribacter atrinae
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3258 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A176TEQ9|A0A176TEQ9_9FLAO Methionine--tRNA ligase OS=Polaribacter atrinae OX=1333662 GN=metG PE=3 SV=1
MM1 pKa = 7.71 LFFCFITFQNINAQLSKK18 pKa = 10.91 KK19 pKa = 10.07 HH20 pKa = 6.51 FIPPLTSTDD29 pKa = 3.33 GFTDD33 pKa = 3.12 QYY35 pKa = 11.33 IYY37 pKa = 10.74 ISTPNNNVSYY47 pKa = 10.72 KK48 pKa = 8.61 ITPVGNLDD56 pKa = 3.17 QTAYY60 pKa = 10.84 SGIVSNNLPIEE71 pKa = 4.1 QPVLNLSGNTDD82 pKa = 4.07 FDD84 pKa = 4.44 NDD86 pKa = 3.96 TQLHH90 pKa = 5.86 IPEE93 pKa = 4.59 SSIIGNKK100 pKa = 6.03 FTNRR104 pKa = 11.84 GFIIEE109 pKa = 4.62 ANDD112 pKa = 4.46 LIYY115 pKa = 10.27 VTIRR119 pKa = 11.84 VRR121 pKa = 11.84 SSQANNGNKK130 pKa = 8.8 NQAGALVSKK139 pKa = 10.59 GNSALGTEE147 pKa = 4.37 FRR149 pKa = 11.84 IGGFIRR155 pKa = 11.84 DD156 pKa = 3.58 GSINGDD162 pKa = 3.46 VTFASIMATEE172 pKa = 5.18 DD173 pKa = 3.29 NTEE176 pKa = 3.97 VTITNATSSSTIININLNEE195 pKa = 4.25 GEE197 pKa = 4.53 SYY199 pKa = 10.43 IIHH202 pKa = 7.07 ANGANSSSIIGNLITSDD219 pKa = 3.32 KK220 pKa = 10.25 PIVVNSGSAYY230 pKa = 10.37 GSFGNNSGNADD241 pKa = 3.59 YY242 pKa = 11.23 GFDD245 pKa = 3.84 QIVGADD251 pKa = 3.93 KK252 pKa = 10.78 IGSEE256 pKa = 4.45 YY257 pKa = 10.6 ILVKK261 pKa = 10.86 GSGNTIEE268 pKa = 4.38 KK269 pKa = 10.46 SLIIAHH275 pKa = 6.68 EE276 pKa = 4.48 DD277 pKa = 3.14 FTEE280 pKa = 4.41 FFINDD285 pKa = 3.49 TSVGTFNAGEE295 pKa = 4.18 YY296 pKa = 10.66 YY297 pKa = 9.24 ITTNSMYY304 pKa = 7.63 TTNGNMYY311 pKa = 10.19 INTNKK316 pKa = 9.96 PVFVYY321 pKa = 10.32 QGVGGLGNNGIPSEE335 pKa = 4.16 ANQGLFFVPPLSCEE349 pKa = 3.84 NRR351 pKa = 11.84 GKK353 pKa = 10.63 VDD355 pKa = 4.67 NIPHH359 pKa = 6.73 IEE361 pKa = 4.25 RR362 pKa = 11.84 IGNEE366 pKa = 4.18 DD367 pKa = 3.26 FTGGISIVTNEE378 pKa = 4.17 DD379 pKa = 3.44 SVVKK383 pKa = 10.67 INGSLITTFSGISGPFLITTKK404 pKa = 10.93 DD405 pKa = 2.99 SGKK408 pKa = 9.67 YY409 pKa = 8.48 DD410 pKa = 3.58 TYY412 pKa = 11.42 KK413 pKa = 9.78 VTGLEE418 pKa = 4.18 GNISIEE424 pKa = 4.2 SSGEE428 pKa = 3.83 LYY430 pKa = 10.5 CAYY433 pKa = 10.46 FNQNGLATSGAFYY446 pKa = 10.75 SGFPSSPEE454 pKa = 3.33 IKK456 pKa = 10.37 FNVAISTLGNCLGNDD471 pKa = 4.52 LEE473 pKa = 4.44 LQAANTEE480 pKa = 4.39 LFDD483 pKa = 3.9 SFKK486 pKa = 10.57 WYY488 pKa = 10.7 FNDD491 pKa = 3.59 GSGYY495 pKa = 9.83 IDD497 pKa = 3.66 TNEE500 pKa = 3.95 TSATLIPSAPGSYY513 pKa = 10.4 QLIGKK518 pKa = 8.73 INCTNTTFKK527 pKa = 10.2 STEE530 pKa = 4.0 IPVSICPDD538 pKa = 3.88 DD539 pKa = 3.97 YY540 pKa = 11.91 DD541 pKa = 5.05 KK542 pKa = 11.78 DD543 pKa = 4.35 GIIDD547 pKa = 4.51 NIDD550 pKa = 2.88 VDD552 pKa = 3.81 IDD554 pKa = 3.54 NDD556 pKa = 4.61 GILNIDD562 pKa = 3.73 EE563 pKa = 4.43 SLGDD567 pKa = 4.13 KK568 pKa = 10.54 IIDD571 pKa = 3.85 LSDD574 pKa = 3.29 INNPTVEE581 pKa = 4.86 DD582 pKa = 4.25 DD583 pKa = 3.55 VTTIITNTFTGDD595 pKa = 2.82 ATGNFTSVANNVTTSNATYY614 pKa = 10.92 SLDD617 pKa = 3.64 FNDD620 pKa = 4.86 NINFKK625 pKa = 9.2 LTQNRR630 pKa = 11.84 TTDD633 pKa = 3.22 HH634 pKa = 6.54 TISNNEE640 pKa = 3.51 YY641 pKa = 10.53 FIIKK645 pKa = 10.46 LSQTEE650 pKa = 4.13 KK651 pKa = 10.94 NITLIDD657 pKa = 4.36 PDD659 pKa = 3.96 NQILIDD665 pKa = 4.18 SNFDD669 pKa = 5.1 DD670 pKa = 4.23 EE671 pKa = 5.88 FEE673 pKa = 5.6 DD674 pKa = 4.7 NFTQFSASVIKK685 pKa = 10.49 FKK687 pKa = 11.11 FKK689 pKa = 11.04 ADD691 pKa = 3.78 LVGSTSTFQFVANQINNISFEE712 pKa = 4.28 HH713 pKa = 6.49 YY714 pKa = 11.01 NDD716 pKa = 3.24 ISSNASTFNGNIGLTCFSLDD736 pKa = 3.26 SDD738 pKa = 4.01 GDD740 pKa = 4.14 GIEE743 pKa = 5.74 DD744 pKa = 4.25 MFDD747 pKa = 4.28 LDD749 pKa = 4.25 TDD751 pKa = 3.61 NDD753 pKa = 5.31 GIPDD757 pKa = 4.28 LYY759 pKa = 10.87 DD760 pKa = 3.89 AYY762 pKa = 10.64 GQEE765 pKa = 3.76 ISLAKK770 pKa = 9.87 TDD772 pKa = 4.09 ANLDD776 pKa = 3.52 GLDD779 pKa = 4.41 DD780 pKa = 3.94 VFEE783 pKa = 5.33 SITTNLDD790 pKa = 3.03 TDD792 pKa = 3.88 GDD794 pKa = 4.35 GVNNYY799 pKa = 9.9 LDD801 pKa = 4.0 YY802 pKa = 11.49 DD803 pKa = 3.92 SDD805 pKa = 3.92 NDD807 pKa = 4.57 GIYY810 pKa = 10.82 DD811 pKa = 3.66 STEE814 pKa = 3.73 ASHH817 pKa = 7.07 GLDD820 pKa = 2.95 TDD822 pKa = 4.07 FNGTIDD828 pKa = 3.64 TFIDD832 pKa = 3.22 INKK835 pKa = 10.02 NGLEE839 pKa = 4.24 DD840 pKa = 3.7 SLEE843 pKa = 4.22 NDD845 pKa = 3.56 TTVQTISLKK854 pKa = 9.4 YY855 pKa = 9.17 TIADD859 pKa = 3.57 TDD861 pKa = 3.37 ADD863 pKa = 4.26 AIFNFIEE870 pKa = 4.69 LDD872 pKa = 3.63 SDD874 pKa = 4.25 ADD876 pKa = 3.67 GCNDD880 pKa = 3.29 VIEE883 pKa = 5.78 AGFTDD888 pKa = 3.86 NNNDD892 pKa = 3.73 GILFANSFSVDD903 pKa = 3.15 EE904 pKa = 4.39 NGKK907 pKa = 9.76 VKK909 pKa = 10.76 NPNDD913 pKa = 4.15 GYY915 pKa = 7.67 TTPNSNYY922 pKa = 6.83 TTSAPIVVTTFTDD935 pKa = 3.44 VTFCEE940 pKa = 4.22 NLTDD944 pKa = 5.71 IITIDD949 pKa = 3.56 SNADD953 pKa = 3.06 SFQWEE958 pKa = 4.49 FSTDD962 pKa = 3.07 GTNWLIINEE971 pKa = 4.62 DD972 pKa = 3.93 DD973 pKa = 4.24 TTDD976 pKa = 3.89 LYY978 pKa = 11.25 QGSTSASLQITNTPFTYY995 pKa = 10.23 DD996 pKa = 2.83 SYY998 pKa = 11.32 QYY1000 pKa = 10.66 RR1001 pKa = 11.84 VALNRR1006 pKa = 11.84 TGNSCGFTSDD1016 pKa = 5.5 AITLTVNPKK1025 pKa = 9.91 PVIEE1029 pKa = 4.54 NNPAALFQCDD1039 pKa = 4.04 TDD1041 pKa = 4.67 SDD1043 pKa = 4.14 LQTTFNLTEE1052 pKa = 4.17 SEE1054 pKa = 3.84 ISITNEE1060 pKa = 3.12 TDD1062 pKa = 3.32 VNFEE1066 pKa = 4.23 YY1067 pKa = 11.09 YY1068 pKa = 9.36 ATEE1071 pKa = 3.93 LDD1073 pKa = 4.88 AINGTPQVADD1083 pKa = 3.37 KK1084 pKa = 10.72 TSYY1087 pKa = 10.81 SVNTNGEE1094 pKa = 4.05 AWVKK1098 pKa = 9.78 TISNTTACYY1107 pKa = 10.27 SISKK1111 pKa = 10.27 INLTVSYY1118 pKa = 10.76 APNEE1122 pKa = 4.34 TFNDD1126 pKa = 3.41 TLYY1129 pKa = 10.97 QCDD1132 pKa = 4.82 DD1133 pKa = 4.27 LLDD1136 pKa = 4.59 LDD1138 pKa = 5.57 GNNTTNNDD1146 pKa = 3.72 DD1147 pKa = 3.69 KK1148 pKa = 11.81 DD1149 pKa = 4.54 GITSFDD1155 pKa = 3.94 LSNISDD1161 pKa = 4.48 KK1162 pKa = 11.02 ISTDD1166 pKa = 2.96 SDD1168 pKa = 3.28 IKK1170 pKa = 10.82 IEE1172 pKa = 4.72 FYY1174 pKa = 10.16 EE1175 pKa = 4.22 TDD1177 pKa = 3.48 DD1178 pKa = 4.74 DD1179 pKa = 4.03 RR1180 pKa = 11.84 TRR1182 pKa = 11.84 SLNEE1186 pKa = 3.26 ITEE1189 pKa = 4.42 TQDD1192 pKa = 3.08 LASYY1196 pKa = 10.37 RR1197 pKa = 11.84 NKK1199 pKa = 10.51 NYY1201 pKa = 10.09 PFTTGNPVTIFYY1213 pKa = 10.91 KK1214 pKa = 10.8 LISITNNNCQGIGEE1228 pKa = 4.97 FYY1230 pKa = 11.2 LEE1232 pKa = 4.24 VNKK1235 pKa = 10.29 IPEE1238 pKa = 4.06 FTVDD1242 pKa = 4.24 GEE1244 pKa = 4.44 APSDD1248 pKa = 4.22 PIIICAEE1255 pKa = 4.21 NIPYY1259 pKa = 8.67 TLNVYY1264 pKa = 8.93 TSNSSYY1270 pKa = 11.06 NYY1272 pKa = 9.35 QWTNEE1277 pKa = 4.03 KK1278 pKa = 10.56 GDD1280 pKa = 3.97 NLGTTQNINISDD1292 pKa = 3.67 AGVYY1296 pKa = 8.72 TVTSSTNSCSRR1307 pKa = 11.84 SRR1309 pKa = 11.84 TITVYY1314 pKa = 10.79 KK1315 pKa = 10.5 SDD1317 pKa = 3.87 FEE1319 pKa = 4.22 NLEE1322 pKa = 4.03 EE1323 pKa = 4.45 SFVTITDD1330 pKa = 3.69 DD1331 pKa = 4.61 LSVINSNLNIRR1342 pKa = 11.84 IDD1344 pKa = 3.45 IPTNPLINEE1353 pKa = 4.3 VFQYY1357 pKa = 11.39 ALEE1360 pKa = 5.29 DD1361 pKa = 3.81 EE1362 pKa = 5.24 DD1363 pKa = 5.17 GMTIRR1368 pKa = 11.84 SFQDD1372 pKa = 3.03 SNVFEE1377 pKa = 5.49 DD1378 pKa = 3.98 IEE1380 pKa = 4.41 GGIYY1384 pKa = 9.98 KK1385 pKa = 10.17 IIVEE1389 pKa = 4.48 SKK1391 pKa = 9.03 NGCGTSEE1398 pKa = 4.47 LLVSVIQFPKK1408 pKa = 10.42 FFTPNGDD1415 pKa = 3.34 RR1416 pKa = 11.84 KK1417 pKa = 10.33 NDD1419 pKa = 2.79 SWGIKK1424 pKa = 9.47 GVSNTLYY1431 pKa = 10.49 QSNSSINIFNRR1442 pKa = 11.84 YY1443 pKa = 7.94 GKK1445 pKa = 10.34 LVAQTTIDD1453 pKa = 3.51 GDD1455 pKa = 3.88 GWDD1458 pKa = 3.44 GTYY1461 pKa = 10.52 NGKK1464 pKa = 9.2 LLPSDD1469 pKa = 4.78 DD1470 pKa = 3.67 YY1471 pKa = 10.8 WYY1473 pKa = 10.48 SIQLIPVNTDD1483 pKa = 2.8 QKK1485 pKa = 10.67 PILKK1489 pKa = 9.71 KK1490 pKa = 10.6 GHH1492 pKa = 6.37 FSLIRR1497 pKa = 11.84 KK1498 pKa = 8.22
Molecular weight: 164.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.795
IPC_protein 3.846
Toseland 3.617
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.77
Rodwell 3.668
Grimsley 3.516
Solomon 3.834
Lehninger 3.795
Nozaki 3.935
DTASelect 4.215
Thurlkill 3.656
EMBOSS 3.783
Sillero 3.961
Patrickios 1.545
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.857
Protein with the highest isoelectric point:
>tr|A0A176TAX6|A0A176TAX6_9FLAO Glutamate-1-semialdehyde 2 1-aminomutase OS=Polaribacter atrinae OX=1333662 GN=hemL PE=3 SV=1
MM1 pKa = 7.51 ARR3 pKa = 11.84 SLKK6 pKa = 10.37 LWLSNTEE13 pKa = 3.61 AEE15 pKa = 4.24 KK16 pKa = 10.87 YY17 pKa = 10.09 IRR19 pKa = 11.84 VSKK22 pKa = 8.47 TLKK25 pKa = 9.92 PYY27 pKa = 10.31 IMKK30 pKa = 10.36 KK31 pKa = 10.36 GVLILIGMFFLVSTIKK47 pKa = 10.91 AEE49 pKa = 4.21 NNKK52 pKa = 9.29 EE53 pKa = 4.1 VPNKK57 pKa = 10.13 IGFNYY62 pKa = 10.23 SYY64 pKa = 10.62 EE65 pKa = 4.08 NTVNFVEE72 pKa = 4.4 RR73 pKa = 11.84 DD74 pKa = 3.33 VEE76 pKa = 4.92 FFIFTNGEE84 pKa = 3.72 FDD86 pKa = 4.53 FNTHH90 pKa = 4.44 YY91 pKa = 10.05 TSNRR95 pKa = 11.84 NEE97 pKa = 3.59 IGVFISRR104 pKa = 11.84 DD105 pKa = 3.17 YY106 pKa = 10.57 RR107 pKa = 11.84 GRR109 pKa = 11.84 IEE111 pKa = 5.14 RR112 pKa = 11.84 IGNSFINYY120 pKa = 8.99 DD121 pKa = 3.08 RR122 pKa = 11.84 YY123 pKa = 11.52 GNVTRR128 pKa = 11.84 IGNVFMKK135 pKa = 9.87 YY136 pKa = 9.52 YY137 pKa = 10.35 RR138 pKa = 11.84 GQLTDD143 pKa = 3.25 VGNLKK148 pKa = 9.66 IRR150 pKa = 11.84 YY151 pKa = 7.53 DD152 pKa = 2.91 HH153 pKa = 6.7 WGNPKK158 pKa = 10.17 FYY160 pKa = 11.29 GNVKK164 pKa = 10.41 DD165 pKa = 5.26 NYY167 pKa = 8.65 YY168 pKa = 10.23 HH169 pKa = 6.91 YY170 pKa = 11.24 NGLRR174 pKa = 11.84 INLNIGNIFEE184 pKa = 4.66 YY185 pKa = 10.38 NNSYY189 pKa = 10.36 FSHH192 pKa = 7.28 RR193 pKa = 11.84 DD194 pKa = 3.41 FARR197 pKa = 11.84 HH198 pKa = 4.36 YY199 pKa = 11.1 SKK201 pKa = 10.38 IRR203 pKa = 11.84 EE204 pKa = 4.14 DD205 pKa = 3.2 NKK207 pKa = 10.1 YY208 pKa = 10.49 YY209 pKa = 10.58 YY210 pKa = 11.05 YY211 pKa = 10.92 KK212 pKa = 10.81 ANPNAKK218 pKa = 9.29 IGNKK222 pKa = 9.81 SKK224 pKa = 10.43 ILRR227 pKa = 11.84 RR228 pKa = 11.84 RR229 pKa = 11.84 KK230 pKa = 8.52 PAPVTRR236 pKa = 11.84 RR237 pKa = 11.84 SNTTYY242 pKa = 10.61 RR243 pKa = 11.84 KK244 pKa = 9.41 PVTINSNRR252 pKa = 11.84 RR253 pKa = 11.84 TVVGKK258 pKa = 10.49 RR259 pKa = 11.84 NNSTTRR265 pKa = 11.84 RR266 pKa = 11.84 STTINTNKK274 pKa = 10.27 NGTIDD279 pKa = 3.38 RR280 pKa = 11.84 RR281 pKa = 11.84 NNSTVTRR288 pKa = 11.84 RR289 pKa = 11.84 PTTVTRR295 pKa = 11.84 GKK297 pKa = 6.52 TTSVKK302 pKa = 10.76 RR303 pKa = 11.84 NDD305 pKa = 3.4 NNSNRR310 pKa = 11.84 KK311 pKa = 6.39 PTVVKK316 pKa = 10.53 RR317 pKa = 11.84 KK318 pKa = 9.14 NEE320 pKa = 4.05 KK321 pKa = 10.21 KK322 pKa = 10.29 KK323 pKa = 10.27 ATTKK327 pKa = 10.58 EE328 pKa = 3.67 EE329 pKa = 4.09 NNRR332 pKa = 11.84 KK333 pKa = 9.23 RR334 pKa = 11.84 RR335 pKa = 11.84 SS336 pKa = 3.28
Molecular weight: 39.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.3
IPC2_protein 10.028
IPC_protein 10.687
Toseland 10.687
ProMoST 10.365
Dawson 10.818
Bjellqvist 10.511
Wikipedia 11.023
Rodwell 11.169
Grimsley 10.877
Solomon 10.891
Lehninger 10.847
Nozaki 10.657
DTASelect 10.511
Thurlkill 10.701
EMBOSS 11.082
Sillero 10.73
Patrickios 10.847
IPC_peptide 10.891
IPC2_peptide 9.282
IPC2.peptide.svr19 8.388
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3258
0
3258
1114503
50
2354
342.1
38.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.044 ± 0.039
0.666 ± 0.012
5.535 ± 0.034
6.685 ± 0.036
5.43 ± 0.036
6.143 ± 0.043
1.641 ± 0.018
8.348 ± 0.043
8.665 ± 0.055
9.183 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.002 ± 0.021
6.592 ± 0.045
3.081 ± 0.021
3.121 ± 0.023
3.072 ± 0.027
6.595 ± 0.035
5.998 ± 0.04
6.156 ± 0.031
1.02 ± 0.015
4.025 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here