Sinosporangium album
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6239 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G7ZRL7|A0A1G7ZRL7_9ACTN DNA-binding transcriptional regulator LysR family OS=Sinosporangium album OX=504805 GN=SAMN05421505_111173 PE=3 SV=1
MM1 pKa = 7.58 KK2 pKa = 10.37 ISVDD6 pKa = 3.42 YY7 pKa = 10.67 DD8 pKa = 3.67 LCEE11 pKa = 4.48 SNALCMEE18 pKa = 4.75 IAPEE22 pKa = 4.03 VFEE25 pKa = 4.93 VRR27 pKa = 11.84 DD28 pKa = 3.84 DD29 pKa = 3.69 NFLYY33 pKa = 11.04 VLDD36 pKa = 4.87 EE37 pKa = 4.68 SPAQAVWPQVEE48 pKa = 4.37 DD49 pKa = 4.03 ALRR52 pKa = 11.84 ACPKK56 pKa = 10.14 GAISIEE62 pKa = 4.27 DD63 pKa = 3.25
Molecular weight: 7.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.769
IPC2_protein 3.834
IPC_protein 3.732
Toseland 3.541
ProMoST 3.808
Dawson 3.719
Bjellqvist 3.948
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.465
Solomon 3.694
Lehninger 3.643
Nozaki 3.859
DTASelect 4.024
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.846
Patrickios 1.863
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|A0A1G8B453|A0A1G8B453_9ACTN LacI family transcriptional regulator OS=Sinosporangium album OX=504805 GN=SAMN05421505_113121 PE=4 SV=1
MM1 pKa = 7.62 LSGPRR6 pKa = 11.84 ARR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 SVKK13 pKa = 10.07 RR14 pKa = 11.84 IQRR17 pKa = 11.84 PVVRR21 pKa = 11.84 TGLRR25 pKa = 11.84 VPLAGEE31 pKa = 4.06 RR32 pKa = 11.84 AMSGAMSGAVPRR44 pKa = 11.84 ALSRR48 pKa = 11.84 TGSLSRR54 pKa = 11.84 TGSLSRR60 pKa = 11.84 TGSLSRR66 pKa = 11.84 TGSLSRR72 pKa = 11.84 TGSVGHH78 pKa = 5.78 TVRR81 pKa = 11.84 GHH83 pKa = 5.27 RR84 pKa = 11.84 RR85 pKa = 11.84 PRR87 pKa = 11.84 RR88 pKa = 11.84 GRR90 pKa = 11.84 IRR92 pKa = 11.84 ARR94 pKa = 11.84 TAEE97 pKa = 4.19 VVKK100 pKa = 10.48 RR101 pKa = 11.84 ATRR104 pKa = 11.84 GAGSGTQFIPKK115 pKa = 9.4 LRR117 pKa = 11.84 LVFLPPSS124 pKa = 3.5
Molecular weight: 13.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.498
IPC2_protein 11.111
IPC_protein 12.72
Toseland 12.881
ProMoST 13.378
Dawson 12.881
Bjellqvist 12.881
Wikipedia 13.364
Rodwell 12.442
Grimsley 12.925
Solomon 13.378
Lehninger 13.29
Nozaki 12.881
DTASelect 12.881
Thurlkill 12.881
EMBOSS 13.393
Sillero 12.881
Patrickios 12.149
IPC_peptide 13.393
IPC2_peptide 12.384
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6239
0
6239
2028968
25
10262
325.2
34.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.298 ± 0.064
0.799 ± 0.009
5.846 ± 0.027
5.639 ± 0.031
2.769 ± 0.017
9.328 ± 0.041
2.324 ± 0.016
3.615 ± 0.029
2.155 ± 0.026
10.267 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.893 ± 0.014
1.79 ± 0.016
6.171 ± 0.035
2.597 ± 0.017
8.303 ± 0.033
5.253 ± 0.022
5.822 ± 0.029
8.592 ± 0.031
1.497 ± 0.013
2.042 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here