Conchiformibius steedae
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2247 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3P2A3Q8|A0A3P2A3Q8_9NEIS Type I-C CRISPR-associated protein Cas7/Csd2 OS=Conchiformibius steedae OX=153493 GN=cas7c PE=4 SV=1
MM1 pKa = 7.71 DD2 pKa = 4.55 TNDD5 pKa = 4.0 FKK7 pKa = 11.15 QTLAPFFWVEE17 pKa = 3.34 HH18 pKa = 6.91 DD19 pKa = 5.26 DD20 pKa = 3.96 YY21 pKa = 11.78 FSVCLYY27 pKa = 10.95 AGDD30 pKa = 3.54 VDD32 pKa = 5.17 AYY34 pKa = 10.8 QAVFDD39 pKa = 4.15 SRR41 pKa = 11.84 ADD43 pKa = 3.45 EE44 pKa = 4.36 GFEE47 pKa = 3.9 GSGYY51 pKa = 10.78 DD52 pKa = 3.41 WTSLAVVFVAEE63 pKa = 4.18 QLPEE67 pKa = 3.73 LAEE70 pKa = 4.42 IIEE73 pKa = 4.5 FDD75 pKa = 3.7 PEE77 pKa = 4.46 AGMFCAYY84 pKa = 10.5 CSDD87 pKa = 3.6 EE88 pKa = 4.16 QALKK92 pKa = 10.19 TFVLAFKK99 pKa = 10.2 EE100 pKa = 3.95 ALEE103 pKa = 4.12 NKK105 pKa = 9.78 EE106 pKa = 4.55 LILDD110 pKa = 3.94 IFSRR114 pKa = 11.84 AEE116 pKa = 3.48 LDD118 pKa = 3.24
Molecular weight: 13.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.719
IPC_protein 3.681
Toseland 3.478
ProMoST 3.795
Dawson 3.668
Bjellqvist 3.859
Wikipedia 3.592
Rodwell 3.516
Grimsley 3.389
Solomon 3.656
Lehninger 3.605
Nozaki 3.783
DTASelect 3.973
Thurlkill 3.528
EMBOSS 3.605
Sillero 3.795
Patrickios 0.896
IPC_peptide 3.643
IPC2_peptide 3.783
IPC2.peptide.svr19 3.772
Protein with the highest isoelectric point:
>tr|A0A3P2A361|A0A3P2A361_9NEIS Glycosyltransferase family 25 protein OS=Conchiformibius steedae OX=153493 GN=EII21_07580 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.14 QPSVTKK11 pKa = 10.56 RR12 pKa = 11.84 KK13 pKa = 7.91 RR14 pKa = 11.84 THH16 pKa = 5.89 GFLVRR21 pKa = 11.84 SKK23 pKa = 9.38 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.87 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2247
0
2247
689593
22
10523
306.9
33.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.312 ± 0.105
1.052 ± 0.027
5.332 ± 0.045
5.673 ± 0.058
3.842 ± 0.046
7.143 ± 0.056
2.576 ± 0.031
5.183 ± 0.052
4.726 ± 0.05
10.247 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.343 ± 0.037
4.042 ± 0.048
4.372 ± 0.042
4.939 ± 0.053
5.682 ± 0.051
5.409 ± 0.049
4.996 ± 0.042
6.794 ± 0.048
1.375 ± 0.026
2.961 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here