Staphylococcus virus 71
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4ZBD2|Q4ZBD2_9CAUD ORF062 OS=Staphylococcus virus 71 OX=320844 PE=4 SV=1
MM1 pKa = 7.59 KK2 pKa = 10.23 KK3 pKa = 10.48 FNVQITYY10 pKa = 9.56 TGMIEE15 pKa = 3.91 EE16 pKa = 4.54 TIEE19 pKa = 4.21 AEE21 pKa = 4.2 SLEE24 pKa = 4.2 EE25 pKa = 4.29 AEE27 pKa = 5.02 NEE29 pKa = 4.01 ARR31 pKa = 11.84 DD32 pKa = 3.74 IAMMEE37 pKa = 4.13 VPFDD41 pKa = 3.87 CDD43 pKa = 2.9 EE44 pKa = 4.32 YY45 pKa = 10.54 EE46 pKa = 4.1 INVEE50 pKa = 4.13 VEE52 pKa = 4.08 QEE54 pKa = 3.56 NDD56 pKa = 2.86
Molecular weight: 6.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.16
IPC2_protein 3.732
IPC_protein 3.579
Toseland 3.439
ProMoST 3.643
Dawson 3.541
Bjellqvist 3.783
Wikipedia 3.427
Rodwell 3.439
Grimsley 3.363
Solomon 3.503
Lehninger 3.452
Nozaki 3.681
DTASelect 3.732
Thurlkill 3.478
EMBOSS 3.452
Sillero 3.694
Patrickios 2.943
IPC_peptide 3.516
IPC2_peptide 3.681
IPC2.peptide.svr19 3.723
Protein with the highest isoelectric point:
>tr|Q4ZBI7|Q4ZBI7_9CAUD ORF046 OS=Staphylococcus virus 71 OX=320844 PE=4 SV=1
MM1 pKa = 7.74 LLILVVNTQYY11 pKa = 10.82 MSIMVLVYY19 pKa = 7.72 MQQVLEE25 pKa = 4.16 VVEE28 pKa = 4.47 RR29 pKa = 11.84 KK30 pKa = 9.58 RR31 pKa = 11.84 YY32 pKa = 9.2 RR33 pKa = 11.84 GRR35 pKa = 11.84 TKK37 pKa = 9.43 MRR39 pKa = 11.84 TVSGILLKK47 pKa = 11.12 DD48 pKa = 3.29 NMLNLFGSRR57 pKa = 11.84 QQ58 pKa = 3.3
Molecular weight: 6.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.315
IPC2_protein 10.028
IPC_protein 10.847
Toseland 10.716
ProMoST 10.496
Dawson 10.847
Bjellqvist 10.584
Wikipedia 11.082
Rodwell 11.038
Grimsley 10.921
Solomon 10.965
Lehninger 10.921
Nozaki 10.672
DTASelect 10.599
Thurlkill 10.73
EMBOSS 11.125
Sillero 10.774
Patrickios 10.833
IPC_peptide 10.965
IPC2_peptide 9.516
IPC2.peptide.svr19 8.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
72
0
72
13903
36
989
193.1
22.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.977 ± 0.372
0.41 ± 0.069
6.243 ± 0.275
7.236 ± 0.413
4.445 ± 0.233
5.783 ± 0.309
1.769 ± 0.168
7.883 ± 0.2
9.128 ± 0.342
7.416 ± 0.268
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.719 ± 0.186
6.531 ± 0.266
2.733 ± 0.193
4.014 ± 0.184
4.064 ± 0.246
5.956 ± 0.26
6.092 ± 0.275
6.128 ± 0.289
1.216 ± 0.149
4.258 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here