Staphylococcus virus 71

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Phietavirus

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q4ZBD2|Q4ZBD2_9CAUD ORF062 OS=Staphylococcus virus 71 OX=320844 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.23KK3 pKa = 10.48FNVQITYY10 pKa = 9.56TGMIEE15 pKa = 3.91EE16 pKa = 4.54TIEE19 pKa = 4.21AEE21 pKa = 4.2SLEE24 pKa = 4.2EE25 pKa = 4.29AEE27 pKa = 5.02NEE29 pKa = 4.01ARR31 pKa = 11.84DD32 pKa = 3.74IAMMEE37 pKa = 4.13VPFDD41 pKa = 3.87CDD43 pKa = 2.9EE44 pKa = 4.32YY45 pKa = 10.54EE46 pKa = 4.1INVEE50 pKa = 4.13VEE52 pKa = 4.08QEE54 pKa = 3.56NDD56 pKa = 2.86

Molecular weight:
6.56 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q4ZBI7|Q4ZBI7_9CAUD ORF046 OS=Staphylococcus virus 71 OX=320844 PE=4 SV=1
MM1 pKa = 7.74LLILVVNTQYY11 pKa = 10.82MSIMVLVYY19 pKa = 7.72MQQVLEE25 pKa = 4.16VVEE28 pKa = 4.47RR29 pKa = 11.84KK30 pKa = 9.58RR31 pKa = 11.84YY32 pKa = 9.2RR33 pKa = 11.84GRR35 pKa = 11.84TKK37 pKa = 9.43MRR39 pKa = 11.84TVSGILLKK47 pKa = 11.12DD48 pKa = 3.29NMLNLFGSRR57 pKa = 11.84QQ58 pKa = 3.3

Molecular weight:
6.88 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

13903

36

989

193.1

22.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.977 ± 0.372

0.41 ± 0.069

6.243 ± 0.275

7.236 ± 0.413

4.445 ± 0.233

5.783 ± 0.309

1.769 ± 0.168

7.883 ± 0.2

9.128 ± 0.342

7.416 ± 0.268

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.719 ± 0.186

6.531 ± 0.266

2.733 ± 0.193

4.014 ± 0.184

4.064 ± 0.246

5.956 ± 0.26

6.092 ± 0.275

6.128 ± 0.289

1.216 ± 0.149

4.258 ± 0.313

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski