Auxenochlorella protothecoides (Green microalga) (Chlorella protothecoides)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae; Chlorellales; Chlorellaceae; Auxenochlorella

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7001 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A087ST42|A0A087ST42_AUXPR VAMP-like protein YKT61 OS=Auxenochlorella protothecoides OX=3075 GN=APUTEX25_001748 PE=3 SV=1
MM1 pKa = 7.68KK2 pKa = 10.05FAQLVIGPAGCGKK15 pKa = 8.66STYY18 pKa = 10.36CEE20 pKa = 4.02TIKK23 pKa = 10.75AHH25 pKa = 7.23CDD27 pKa = 3.12TIGRR31 pKa = 11.84SVHH34 pKa = 5.76VVNLDD39 pKa = 3.35PAAEE43 pKa = 4.08HH44 pKa = 6.29FNYY47 pKa = 9.7PVSIDD52 pKa = 3.51VRR54 pKa = 11.84DD55 pKa = 4.95LITLDD60 pKa = 4.19DD61 pKa = 3.93VAEE64 pKa = 4.24EE65 pKa = 4.7LDD67 pKa = 3.7LGPNGGLLYY76 pKa = 10.95CMEE79 pKa = 4.44FLEE82 pKa = 5.96DD83 pKa = 5.32NLSDD87 pKa = 3.37WLEE90 pKa = 4.23EE91 pKa = 3.85EE92 pKa = 4.14LQGYY96 pKa = 9.44GEE98 pKa = 4.83DD99 pKa = 4.65DD100 pKa = 4.02YY101 pKa = 12.11LLFDD105 pKa = 4.49CPGQIEE111 pKa = 5.09LYY113 pKa = 9.42SHH115 pKa = 6.48VSVFRR120 pKa = 11.84TFTEE124 pKa = 4.16FLKK127 pKa = 10.66RR128 pKa = 11.84DD129 pKa = 3.18GWNICAVYY137 pKa = 10.75CMDD140 pKa = 3.59SQFVAEE146 pKa = 4.58PPKK149 pKa = 10.57FIAGCLSALSAMVQLEE165 pKa = 4.31LPHH168 pKa = 8.23VNMLTKK174 pKa = 10.35VDD176 pKa = 4.27LVQDD180 pKa = 3.68KK181 pKa = 11.15DD182 pKa = 3.82SLEE185 pKa = 4.48SYY187 pKa = 10.01LFPDD191 pKa = 3.75ALEE194 pKa = 4.38LRR196 pKa = 11.84HH197 pKa = 6.58ALGEE201 pKa = 4.37STGPRR206 pKa = 11.84FSALNDD212 pKa = 3.6VIVNLLDD219 pKa = 3.45EE220 pKa = 4.56FAMVSFTPLDD230 pKa = 3.57IGDD233 pKa = 3.98EE234 pKa = 4.19EE235 pKa = 5.1SIEE238 pKa = 4.17TALFQVDD245 pKa = 3.54SAIQYY250 pKa = 10.98GEE252 pKa = 4.19DD253 pKa = 3.02QDD255 pKa = 4.66VRR257 pKa = 11.84QKK259 pKa = 11.21EE260 pKa = 4.28YY261 pKa = 11.32GDD263 pKa = 4.15FPDD266 pKa = 4.44EE267 pKa = 3.73EE268 pKa = 5.29APAHH272 pKa = 5.39EE273 pKa = 5.66AIDD276 pKa = 3.81FF277 pKa = 4.19

Molecular weight: 30.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A087SIX0|A0A087SIX0_AUXPR DUF1995 domain-containing protein OS=Auxenochlorella protothecoides OX=3075 GN=APUTEX25_004025 PE=4 SV=1
MM1 pKa = 7.37ARR3 pKa = 11.84LAATHH8 pKa = 6.6LAQRR12 pKa = 11.84GRR14 pKa = 11.84RR15 pKa = 11.84SSLRR19 pKa = 11.84VWISPTLTSGSRR31 pKa = 11.84HH32 pKa = 5.36AFWRR36 pKa = 11.84TSWRR40 pKa = 11.84RR41 pKa = 11.84GRR43 pKa = 11.84VLGRR47 pKa = 11.84GRR49 pKa = 11.84RR50 pKa = 11.84PPLPPWSRR58 pKa = 11.84AAA60 pKa = 3.72

Molecular weight: 6.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6999

2

7001

2763953

49

16440

394.8

42.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.692 ± 0.061

1.513 ± 0.012

4.951 ± 0.018

5.862 ± 0.03

2.922 ± 0.017

8.823 ± 0.03

2.311 ± 0.014

2.96 ± 0.021

3.066 ± 0.028

10.716 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.953 ± 0.013

2.021 ± 0.016

6.257 ± 0.026

3.662 ± 0.018

7.136 ± 0.028

6.492 ± 0.023

4.881 ± 0.019

6.974 ± 0.024

1.48 ± 0.012

2.059 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski