Amino acid dipepetide frequency for Auxenochlorella protothecoides (Green microalga) (Chlorella protothecoides)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	24.1AlaAla: 24.1 ± 0.226	2.252AlaCys: 2.252 ± 0.037	5.85AlaAsp: 5.85 ± 0.052	7.836AlaGlu: 7.836 ± 0.08	3.707AlaPhe: 3.707 ± 0.042	12.772AlaGly: 12.772 ± 0.112	2.687AlaHis: 2.687 ± 0.032	3.301AlaIle: 3.301 ± 0.039	3.5AlaLys: 3.5 ± 0.041	14.984AlaLeu: 14.984 ± 0.124	2.685AlaMet: 2.685 ± 0.033	2.031AlaAsn: 2.031 ± 0.031	7.813AlaPro: 7.813 ± 0.073	4.794AlaGln: 4.794 ± 0.046	9.782AlaArg: 9.782 ± 0.08	9.12AlaSer: 9.12 ± 0.074	6.206AlaThr: 6.206 ± 0.058	9.063AlaVal: 9.063 ± 0.071	2.126AlaTrp: 2.126 ± 0.034	2.305AlaTyr: 2.305 ± 0.032	0.0AlaXaa: 0.0 ± 0.0
Cys	1.717CysAla: 1.717 ± 0.029	0.345CysCys: 0.345 ± 0.012	0.657CysAsp: 0.657 ± 0.017	0.647CysGlu: 0.647 ± 0.016	0.468CysPhe: 0.468 ± 0.011	1.274CysGly: 1.274 ± 0.025	0.387CysHis: 0.387 ± 0.013	0.563CysIle: 0.563 ± 0.014	0.415CysLys: 0.415 ± 0.014	1.735CysLeu: 1.735 ± 0.028	0.366CysMet: 0.366 ± 0.012	0.376CysAsn: 0.376 ± 0.012	0.999CysPro: 0.999 ± 0.022	0.515CysGln: 0.515 ± 0.013	1.089CysArg: 1.089 ± 0.024	1.097CysSer: 1.097 ± 0.026	0.888CysThr: 0.888 ± 0.02	1.042CysVal: 1.042 ± 0.02	0.26CysTrp: 0.26 ± 0.011	0.287CysTyr: 0.287 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.163AspAla: 7.163 ± 0.058	0.65AspCys: 0.65 ± 0.015	2.652AspAsp: 2.652 ± 0.041	3.17AspGlu: 3.17 ± 0.039	1.645AspPhe: 1.645 ± 0.028	4.428AspGly: 4.428 ± 0.048	0.916AspHis: 0.916 ± 0.018	1.763AspIle: 1.763 ± 0.026	1.353AspLys: 1.353 ± 0.024	5.303AspLeu: 5.303 ± 0.043	0.993AspMet: 0.993 ± 0.019	0.868AspAsn: 0.868 ± 0.017	3.552AspPro: 3.552 ± 0.039	1.337AspGln: 1.337 ± 0.024	2.936AspArg: 2.936 ± 0.039	2.717AspSer: 2.717 ± 0.034	2.252AspThr: 2.252 ± 0.028	3.885AspVal: 3.885 ± 0.037	0.844AspTrp: 0.844 ± 0.019	1.083AspTyr: 1.083 ± 0.026	0.0AspXaa: 0.0 ± 0.0
Glu	9.243GluAla: 9.243 ± 0.087	0.664GluCys: 0.664 ± 0.016	3.35GluAsp: 3.35 ± 0.039	5.239GluGlu: 5.239 ± 0.073	1.476GluPhe: 1.476 ± 0.027	5.466GluGly: 5.466 ± 0.043	1.195GluHis: 1.195 ± 0.023	1.567GluIle: 1.567 ± 0.025	1.784GluLys: 1.784 ± 0.036	5.872GluLeu: 5.872 ± 0.058	1.018GluMet: 1.018 ± 0.019	1.031GluAsn: 1.031 ± 0.021	2.672GluPro: 2.672 ± 0.035	1.979GluGln: 1.979 ± 0.03	4.348GluArg: 4.348 ± 0.051	2.966GluSer: 2.966 ± 0.032	2.343GluThr: 2.343 ± 0.029	4.418GluVal: 4.418 ± 0.044	0.797GluTrp: 0.797 ± 0.017	1.191GluTyr: 1.191 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	2.952PheAla: 2.952 ± 0.034	0.503PheCys: 0.503 ± 0.015	1.729PheAsp: 1.729 ± 0.033	1.543PheGlu: 1.543 ± 0.025	1.177PhePhe: 1.177 ± 0.02	2.415PheGly: 2.415 ± 0.037	0.697PheHis: 0.697 ± 0.015	1.019PheIle: 1.019 ± 0.022	1.056PheLys: 1.056 ± 0.019	3.207PheLeu: 3.207 ± 0.041	0.658PheMet: 0.658 ± 0.018	0.832PheAsn: 0.832 ± 0.019	1.553PhePro: 1.553 ± 0.025	1.172PheGln: 1.172 ± 0.021	1.803PheArg: 1.803 ± 0.028	2.01PheSer: 2.01 ± 0.029	1.507PheThr: 1.507 ± 0.024	2.125PheVal: 2.125 ± 0.031	0.479PheTrp: 0.479 ± 0.014	0.779PheTyr: 0.779 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	11.474GlyAla: 11.474 ± 0.107	1.263GlyCys: 1.263 ± 0.025	4.206GlyAsp: 4.206 ± 0.045	4.986GlyGlu: 4.986 ± 0.048	2.522GlyPhe: 2.522 ± 0.032	8.803GlyGly: 8.803 ± 0.084	1.898GlyHis: 1.898 ± 0.029	2.666GlyIle: 2.666 ± 0.039	2.907GlyLys: 2.907 ± 0.038	8.78GlyLeu: 8.78 ± 0.063	1.913GlyMet: 1.913 ± 0.029	1.775GlyAsn: 1.775 ± 0.028	5.328GlyPro: 5.328 ± 0.056	2.998GlyGln: 2.998 ± 0.033	6.169GlyArg: 6.169 ± 0.056	6.258GlySer: 6.258 ± 0.057	4.61GlyThr: 4.61 ± 0.043	6.545GlyVal: 6.545 ± 0.057	1.444GlyTrp: 1.444 ± 0.026	1.683GlyTyr: 1.683 ± 0.03	0.001GlyXaa: 0.001 ± 0.001
His	3.263HisAla: 3.263 ± 0.041	0.387HisCys: 0.387 ± 0.012	1.05HisAsp: 1.05 ± 0.019	1.009HisGlu: 1.009 ± 0.021	0.697HisPhe: 0.697 ± 0.015	2.071HisGly: 2.071 ± 0.03	0.608HisHis: 0.608 ± 0.016	0.785HisIle: 0.785 ± 0.017	0.605HisLys: 0.605 ± 0.014	2.406HisLeu: 2.406 ± 0.03	0.49HisMet: 0.49 ± 0.014	0.46HisAsn: 0.46 ± 0.013	1.734HisPro: 1.734 ± 0.027	0.73HisGln: 0.73 ± 0.016	1.628HisArg: 1.628 ± 0.022	1.472HisSer: 1.472 ± 0.022	1.176HisThr: 1.176 ± 0.022	1.679HisVal: 1.679 ± 0.024	0.36HisTrp: 0.36 ± 0.013	0.498HisTyr: 0.498 ± 0.014	0.001HisXaa: 0.001 ± 0.001
Ile	3.165IleAla: 3.165 ± 0.037	0.486IleCys: 0.486 ± 0.013	1.603IleAsp: 1.603 ± 0.028	1.478IleGlu: 1.478 ± 0.023	1.047IlePhe: 1.047 ± 0.023	2.119IleGly: 2.119 ± 0.032	0.719IleHis: 0.719 ± 0.018	1.151IleIle: 1.151 ± 0.028	1.23IleLys: 1.23 ± 0.023	3.093IleLeu: 3.093 ± 0.042	0.714IleMet: 0.714 ± 0.018	0.912IleAsn: 0.912 ± 0.021	1.869IlePro: 1.869 ± 0.028	1.198IleGln: 1.198 ± 0.023	1.845IleArg: 1.845 ± 0.031	1.98IleSer: 1.98 ± 0.029	1.665IleThr: 1.665 ± 0.029	2.245IleVal: 2.245 ± 0.032	0.398IleTrp: 0.398 ± 0.012	0.682IleTyr: 0.682 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	3.903LysAla: 3.903 ± 0.046	0.314LysCys: 0.314 ± 0.01	1.571LysAsp: 1.571 ± 0.027	2.053LysGlu: 2.053 ± 0.036	0.851LysPhe: 0.851 ± 0.016	2.401LysGly: 2.401 ± 0.037	0.732LysHis: 0.732 ± 0.018	1.014LysIle: 1.014 ± 0.021	1.675LysLys: 1.675 ± 0.04	3.069LysLeu: 3.069 ± 0.039	0.577LysMet: 0.577 ± 0.015	0.754LysAsn: 0.754 ± 0.02	1.664LysPro: 1.664 ± 0.024	1.177LysGln: 1.177 ± 0.024	2.323LysArg: 2.323 ± 0.036	1.664LysSer: 1.664 ± 0.03	1.589LysThr: 1.589 ± 0.027	2.136LysVal: 2.136 ± 0.033	0.378LysTrp: 0.378 ± 0.012	0.808LysTyr: 0.808 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	15.188LeuAla: 15.188 ± 0.121	1.661LeuCys: 1.661 ± 0.03	5.696LeuAsp: 5.696 ± 0.048	6.735LeuGlu: 6.735 ± 0.062	2.84LeuPhe: 2.84 ± 0.038	8.87LeuGly: 8.87 ± 0.066	2.766LeuHis: 2.766 ± 0.031	2.654LeuIle: 2.654 ± 0.038	3.173LeuLys: 3.173 ± 0.038	11.683LeuLeu: 11.683 ± 0.101	1.839LeuMet: 1.839 ± 0.025	2.143LeuAsn: 2.143 ± 0.03	6.692LeuPro: 6.692 ± 0.057	4.522LeuGln: 4.522 ± 0.047	8.333LeuArg: 8.333 ± 0.072	6.682LeuSer: 6.682 ± 0.056	5.208LeuThr: 5.208 ± 0.051	7.15LeuVal: 7.15 ± 0.049	1.3LeuTrp: 1.3 ± 0.027	2.024LeuTyr: 2.024 ± 0.031	0.001LeuXaa: 0.001 ± 0.001
Met	2.568MetAla: 2.568 ± 0.034	0.25MetCys: 0.25 ± 0.009	1.06MetAsp: 1.06 ± 0.021	1.22MetGlu: 1.22 ± 0.021	0.53MetPhe: 0.53 ± 0.014	1.589MetGly: 1.589 ± 0.025	0.544MetHis: 0.544 ± 0.015	0.508MetIle: 0.508 ± 0.016	0.584MetLys: 0.584 ± 0.016	2.227MetLeu: 2.227 ± 0.03	0.387MetMet: 0.387 ± 0.014	0.407MetAsn: 0.407 ± 0.012	1.147MetPro: 1.147 ± 0.019	0.916MetGln: 0.916 ± 0.018	1.369MetArg: 1.369 ± 0.023	1.237MetSer: 1.237 ± 0.022	0.975MetThr: 0.975 ± 0.022	1.35MetVal: 1.35 ± 0.023	0.219MetTrp: 0.219 ± 0.01	0.45MetTyr: 0.45 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.282AsnAla: 2.282 ± 0.031	0.315AsnCys: 0.315 ± 0.012	0.929AsnAsp: 0.929 ± 0.018	0.966AsnGlu: 0.966 ± 0.02	0.758AsnPhe: 0.758 ± 0.016	1.621AsnGly: 1.621 ± 0.029	0.428AsnHis: 0.428 ± 0.014	0.942AsnIle: 0.942 ± 0.02	0.794AsnLys: 0.794 ± 0.016	2.152AsnLeu: 2.152 ± 0.036	0.446AsnMet: 0.446 ± 0.014	0.59AsnAsn: 0.59 ± 0.017	1.525AsnPro: 1.525 ± 0.025	0.66AsnGln: 0.66 ± 0.018	1.17AsnArg: 1.17 ± 0.02	1.227AsnSer: 1.227 ± 0.023	1.047AsnThr: 1.047 ± 0.021	1.509AsnVal: 1.509 ± 0.028	0.325AsnTrp: 0.325 ± 0.011	0.529AsnTyr: 0.529 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	8.866ProAla: 8.866 ± 0.076	0.859ProCys: 0.859 ± 0.018	3.202ProAsp: 3.202 ± 0.038	3.676ProGlu: 3.676 ± 0.038	1.707ProPhe: 1.707 ± 0.026	6.868ProGly: 6.868 ± 0.061	1.402ProHis: 1.402 ± 0.027	1.56ProIle: 1.56 ± 0.028	1.513ProLys: 1.513 ± 0.026	5.657ProLeu: 5.657 ± 0.054	0.925ProMet: 0.925 ± 0.016	1.13ProAsn: 1.13 ± 0.021	5.371ProPro: 5.371 ± 0.08	1.945ProGln: 1.945 ± 0.027	4.235ProArg: 4.235 ± 0.041	4.991ProSer: 4.991 ± 0.054	3.06ProThr: 3.06 ± 0.04	3.671ProVal: 3.671 ± 0.031	0.853ProTrp: 0.853 ± 0.021	1.072ProTyr: 1.072 ± 0.02	0.003ProXaa: 0.003 ± 0.001
Gln	5.438GlnAla: 5.438 ± 0.054	0.444GlnCys: 0.444 ± 0.014	1.704GlnAsp: 1.704 ± 0.026	2.217GlnGlu: 2.217 ± 0.028	0.873GlnPhe: 0.873 ± 0.02	3.18GlnGly: 3.18 ± 0.04	1.009GlnHis: 1.009 ± 0.023	0.959GlnIle: 0.959 ± 0.022	1.042GlnLys: 1.042 ± 0.022	3.959GlnLeu: 3.959 ± 0.04	0.646GlnMet: 0.646 ± 0.016	0.666GlnAsn: 0.666 ± 0.017	2.172GlnPro: 2.172 ± 0.032	1.737GlnGln: 1.737 ± 0.033	3.228GlnArg: 3.228 ± 0.034	1.977GlnSer: 1.977 ± 0.024	1.668GlnThr: 1.668 ± 0.028	2.327GlnVal: 2.327 ± 0.035	0.474GlnTrp: 0.474 ± 0.013	0.9GlnTyr: 0.9 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	9.264ArgAla: 9.264 ± 0.083	1.212ArgCys: 1.212 ± 0.021	3.445ArgAsp: 3.445 ± 0.036	4.253ArgGlu: 4.253 ± 0.049	2.068ArgPhe: 2.068 ± 0.027	6.006ArgGly: 6.006 ± 0.053	1.903ArgHis: 1.903 ± 0.031	2.027ArgIle: 2.027 ± 0.032	2.201ArgLys: 2.201 ± 0.029	8.079ArgLeu: 8.079 ± 0.067	1.436ArgMet: 1.436 ± 0.024	1.267ArgAsn: 1.267 ± 0.022	4.211ArgPro: 4.211 ± 0.048	2.793ArgGln: 2.793 ± 0.034	6.356ArgArg: 6.356 ± 0.07	4.309ArgSer: 4.309 ± 0.044	3.099ArgThr: 3.099 ± 0.034	4.823ArgVal: 4.823 ± 0.044	1.136ArgTrp: 1.136 ± 0.021	1.468ArgTyr: 1.468 ± 0.025	0.0ArgXaa: 0.0 ± 0.0
Ser	7.714SerAla: 7.714 ± 0.068	1.003SerCys: 1.003 ± 0.022	2.973SerAsp: 2.973 ± 0.037	3.106SerGlu: 3.106 ± 0.037	1.967SerPhe: 1.967 ± 0.03	6.044SerGly: 6.044 ± 0.061	1.389SerHis: 1.389 ± 0.021	2.189SerIle: 2.189 ± 0.035	2.021SerLys: 2.021 ± 0.034	7.267SerLeu: 7.267 ± 0.058	1.391SerMet: 1.391 ± 0.026	1.385SerAsn: 1.385 ± 0.023	4.543SerPro: 4.543 ± 0.054	2.307SerGln: 2.307 ± 0.031	4.352SerArg: 4.352 ± 0.037	5.213SerSer: 5.213 ± 0.061	3.458SerThr: 3.458 ± 0.038	4.369SerVal: 4.369 ± 0.047	0.995SerTrp: 0.995 ± 0.021	1.231SerTyr: 1.231 ± 0.023	0.0SerXaa: 0.0 ± 0.0
Thr	5.88ThrAla: 5.88 ± 0.064	0.839ThrCys: 0.839 ± 0.019	2.062ThrAsp: 2.062 ± 0.03	2.273ThrGlu: 2.273 ± 0.035	1.561ThrPhe: 1.561 ± 0.026	4.25ThrGly: 4.25 ± 0.044	1.135ThrHis: 1.135 ± 0.017	1.63ThrIle: 1.63 ± 0.028	1.416ThrLys: 1.416 ± 0.027	6.043ThrLeu: 6.043 ± 0.049	0.914ThrMet: 0.914 ± 0.02	1.038ThrAsn: 1.038 ± 0.021	3.755ThrPro: 3.755 ± 0.039	1.71ThrGln: 1.71 ± 0.027	3.056ThrArg: 3.056 ± 0.035	3.522ThrSer: 3.522 ± 0.038	2.528ThrThr: 2.528 ± 0.039	3.38ThrVal: 3.38 ± 0.034	0.737ThrTrp: 0.737 ± 0.019	1.076ThrTyr: 1.076 ± 0.025	0.0ThrXaa: 0.0 ± 0.0
Val	8.925ValAla: 8.925 ± 0.062	1.069ValCys: 1.069 ± 0.022	3.747ValAsp: 3.747 ± 0.04	4.246ValGlu: 4.246 ± 0.04	2.151ValPhe: 2.151 ± 0.032	5.265ValGly: 5.265 ± 0.052	1.758ValHis: 1.758 ± 0.027	2.109ValIle: 2.109 ± 0.031	2.172ValLys: 2.172 ± 0.034	7.926ValLeu: 7.926 ± 0.068	1.348ValMet: 1.348 ± 0.021	1.497ValAsn: 1.497 ± 0.025	4.271ValPro: 4.271 ± 0.047	2.758ValGln: 2.758 ± 0.028	4.639ValArg: 4.639 ± 0.038	4.153ValSer: 4.153 ± 0.043	3.583ValThr: 3.583 ± 0.037	5.498ValVal: 5.498 ± 0.057	1.071ValTrp: 1.071 ± 0.025	1.555ValTyr: 1.555 ± 0.023	0.001ValXaa: 0.001 ± 0.001
Trp	1.672TrpAla: 1.672 ± 0.027	0.217TrpCys: 0.217 ± 0.009	0.861TrpAsp: 0.861 ± 0.019	0.853TrpGlu: 0.853 ± 0.018	0.403TrpPhe: 0.403 ± 0.012	1.143TrpGly: 1.143 ± 0.022	0.426TrpHis: 0.426 ± 0.014	0.418TrpIle: 0.418 ± 0.013	0.475TrpLys: 0.475 ± 0.013	1.548TrpLeu: 1.548 ± 0.026	0.329TrpMet: 0.329 ± 0.012	0.38TrpAsn: 0.38 ± 0.012	0.687TrpPro: 0.687 ± 0.015	0.675TrpGln: 0.675 ± 0.015	1.313TrpArg: 1.313 ± 0.024	0.976TrpSer: 0.976 ± 0.019	0.779TrpThr: 0.779 ± 0.017	1.029TrpVal: 1.029 ± 0.023	0.283TrpTrp: 0.283 ± 0.011	0.335TrpTyr: 0.335 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.136TyrAla: 2.136 ± 0.032	0.397TyrCys: 0.397 ± 0.013	1.165TyrAsp: 1.165 ± 0.023	1.11TyrGlu: 1.11 ± 0.021	0.767TyrPhe: 0.767 ± 0.019	1.643TyrGly: 1.643 ± 0.031	0.51TyrHis: 0.51 ± 0.013	0.774TyrIle: 0.774 ± 0.02	0.741TyrLys: 0.741 ± 0.018	2.166TyrLeu: 2.166 ± 0.033	0.46TyrMet: 0.46 ± 0.013	0.672TyrAsn: 0.672 ± 0.018	1.007TyrPro: 1.007 ± 0.022	0.695TyrGln: 0.695 ± 0.016	1.38TyrArg: 1.38 ± 0.025	1.346TyrSer: 1.346 ± 0.021	1.164TyrThr: 1.164 ± 0.022	1.499TyrVal: 1.499 ± 0.024	0.325TyrTrp: 0.325 ± 0.011	0.631TyrTyr: 0.631 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	2.708XaaXaa: 2.708 ± 0.575

Statistics based on 7001 proteins (2763953 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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