Erwinia tracheiphila
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3807 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M2KIT1|A0A0M2KIT1_9GAMM DNA-binding transcriptional regulator CpxR OS=Erwinia tracheiphila OX=65700 GN=cpxR PE=4 SV=1
MM1 pKa = 7.75 SYY3 pKa = 10.59 QEE5 pKa = 4.66 TILTQYY11 pKa = 10.25 SASEE15 pKa = 4.43 KK16 pKa = 9.87 ILSIIDD22 pKa = 3.62 TFNQALSLDD31 pKa = 4.0 DD32 pKa = 4.02 FTDD35 pKa = 3.64 EE36 pKa = 4.82 FIKK39 pKa = 10.74 DD40 pKa = 3.25 VWEE43 pKa = 4.15 VTTCGTFGLDD53 pKa = 3.06 VWGKK57 pKa = 8.36 IVNVSRR63 pKa = 11.84 YY64 pKa = 8.01 IQADD68 pKa = 2.97 IDD70 pKa = 3.73 SDD72 pKa = 3.82 RR73 pKa = 11.84 FGFSEE78 pKa = 4.65 ADD80 pKa = 3.44 SGDD83 pKa = 4.43 DD84 pKa = 3.6 YY85 pKa = 11.45 PSPFNDD91 pKa = 2.95 QPFYY95 pKa = 11.46 GGIQEE100 pKa = 4.32 TEE102 pKa = 4.25 TVKK105 pKa = 11.04 LSDD108 pKa = 3.38 DD109 pKa = 3.78 AYY111 pKa = 10.16 RR112 pKa = 11.84 TLVLCKK118 pKa = 9.96 AFSNISIATISEE130 pKa = 3.81 INRR133 pKa = 11.84 FLTILFNGRR142 pKa = 11.84 GRR144 pKa = 11.84 AYY146 pKa = 10.52 CVDD149 pKa = 3.84 YY150 pKa = 11.35 GDD152 pKa = 3.35 MRR154 pKa = 11.84 MGIFCEE160 pKa = 4.61 FEE162 pKa = 3.76 LAPYY166 pKa = 9.27 EE167 pKa = 4.1 ISILKK172 pKa = 10.44 NYY174 pKa = 9.84 DD175 pKa = 3.3 VLPIPSGVLVTAHH188 pKa = 6.42 QIAPPYY194 pKa = 10.33 FGFSDD199 pKa = 4.08 DD200 pKa = 4.69 SYY202 pKa = 10.81 PFNDD206 pKa = 3.3 GTFFRR211 pKa = 11.84 DD212 pKa = 2.93 II213 pKa = 3.99
Molecular weight: 24.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.961
IPC_protein 3.961
Toseland 3.745
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.897
Rodwell 3.783
Grimsley 3.656
Solomon 3.948
Lehninger 3.91
Nozaki 4.062
DTASelect 4.329
Thurlkill 3.795
EMBOSS 3.91
Sillero 4.088
Patrickios 1.189
IPC_peptide 3.948
IPC2_peptide 4.062
IPC2.peptide.svr19 3.949
Protein with the highest isoelectric point:
>tr|A0A0M2KH31|A0A0M2KH31_9GAMM Translocation and assembly module TamB OS=Erwinia tracheiphila OX=65700 GN=SY86_13740 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.42 NGRR28 pKa = 11.84 QVIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.14 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTVSKK46 pKa = 10.99
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3807
0
3807
1026315
34
3049
269.6
29.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.2 ± 0.05
1.159 ± 0.016
5.247 ± 0.035
5.644 ± 0.044
3.765 ± 0.028
7.169 ± 0.05
2.329 ± 0.024
5.784 ± 0.035
4.663 ± 0.046
10.568 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.745 ± 0.019
3.964 ± 0.035
4.302 ± 0.029
4.556 ± 0.035
6.065 ± 0.04
6.318 ± 0.038
5.363 ± 0.028
6.843 ± 0.031
1.494 ± 0.018
2.821 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here