Defluviimonas aquaemixtae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Defluviimonas

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4129 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R8BM21|A0A2R8BM21_9RHOB PNPLA domain-containing protein OS=Defluviimonas aquaemixtae OX=1542388 GN=DEA8626_03508 PE=4 SV=1
MM1 pKa = 6.74STAIKK6 pKa = 10.35FDD8 pKa = 4.04FSQAAGEE15 pKa = 4.36EE16 pKa = 4.49IEE18 pKa = 4.79SSLFGANMIFTKK30 pKa = 10.83NILDD34 pKa = 4.06NNSSPTLDD42 pKa = 3.6NLNVSNIRR50 pKa = 11.84YY51 pKa = 8.6PGGSVTEE58 pKa = 4.25FNFDD62 pKa = 3.45YY63 pKa = 10.42RR64 pKa = 11.84NPDD67 pKa = 2.66IAVNDD72 pKa = 3.68NSLHH76 pKa = 6.13YY77 pKa = 11.26DD78 pKa = 3.42NDD80 pKa = 3.21QDD82 pKa = 4.12TAPTPLLPGEE92 pKa = 4.48FVPLTSFLDD101 pKa = 3.73WVEE104 pKa = 4.25STGKK108 pKa = 10.04SATVVLPTRR117 pKa = 11.84NLVDD121 pKa = 3.52QSTQQGTLPRR131 pKa = 11.84AINIEE136 pKa = 4.26AIEE139 pKa = 4.16DD140 pKa = 3.71LKK142 pKa = 11.45KK143 pKa = 10.78FLLDD147 pKa = 3.85LLSDD151 pKa = 3.54VDD153 pKa = 4.53SNGNAMQNADD163 pKa = 3.43IAALEE168 pKa = 4.22IGNEE172 pKa = 4.09YY173 pKa = 9.91WSLGSMTAAEE183 pKa = 4.22YY184 pKa = 10.62GAVVDD189 pKa = 4.76AVIAVIEE196 pKa = 4.53GVFEE200 pKa = 3.96EE201 pKa = 5.0LGISSQEE208 pKa = 3.97APDD211 pKa = 3.85VLVQMGSPWGVEE223 pKa = 4.24FQSGGAYY230 pKa = 10.3YY231 pKa = 10.6GITADD236 pKa = 3.55SPQYY240 pKa = 10.97ILDD243 pKa = 3.67QYY245 pKa = 11.62GLTDD249 pKa = 4.27SDD251 pKa = 4.07FLNDD255 pKa = 5.59GSLKK259 pKa = 9.7WLSKK263 pKa = 10.64INLVNLDD270 pKa = 4.13IIRR273 pKa = 11.84GISEE277 pKa = 4.13DD278 pKa = 3.29SRR280 pKa = 11.84NSVDD284 pKa = 3.8GVVDD288 pKa = 3.41HH289 pKa = 6.7YY290 pKa = 11.57YY291 pKa = 11.04YY292 pKa = 11.06HH293 pKa = 6.12NTDD296 pKa = 3.0NALSYY301 pKa = 10.88TSGSINYY308 pKa = 8.7IDD310 pKa = 4.2RR311 pKa = 11.84DD312 pKa = 3.77YY313 pKa = 11.46AIWSSNFGRR322 pKa = 11.84EE323 pKa = 3.88LDD325 pKa = 3.67LHH327 pKa = 4.91ITEE330 pKa = 4.92WNVKK334 pKa = 7.08TANLNQLGLKK344 pKa = 9.1GAGVILEE351 pKa = 4.09QVEE354 pKa = 4.14NLVRR358 pKa = 11.84LGVDD362 pKa = 3.45SAFVWPYY369 pKa = 9.29QHH371 pKa = 6.24NTRR374 pKa = 11.84NDD376 pKa = 3.59LAGSPGDD383 pKa = 3.98DD384 pKa = 3.36TSLTPLGAAFKK395 pKa = 10.31LAAEE399 pKa = 4.34SLVGTRR405 pKa = 11.84RR406 pKa = 11.84LDD408 pKa = 3.6SNISGGEE415 pKa = 4.04LEE417 pKa = 4.43VNAYY421 pKa = 9.12SASEE425 pKa = 3.98KK426 pKa = 10.58FVFFVSSRR434 pKa = 11.84SDD436 pKa = 3.23TQQSIEE442 pKa = 4.17IDD444 pKa = 3.57FGDD447 pKa = 3.88LVSSYY452 pKa = 11.14VSVSGVKK459 pKa = 9.87IGVDD463 pKa = 3.94LSTSDD468 pKa = 3.24GRR470 pKa = 11.84HH471 pKa = 4.48WTPSGISQVEE481 pKa = 4.12YY482 pKa = 10.58FNEE485 pKa = 4.05SDD487 pKa = 3.66VQAQLSQYY495 pKa = 11.02SEE497 pKa = 5.05DD498 pKa = 4.1EE499 pKa = 4.48LGTAANVSFVLDD511 pKa = 4.02PYY513 pKa = 11.3EE514 pKa = 4.34VMRR517 pKa = 11.84LEE519 pKa = 4.25FVLDD523 pKa = 3.75AARR526 pKa = 11.84QFSGASGNDD535 pKa = 3.11TWSGGAGYY543 pKa = 10.36DD544 pKa = 3.46VAFGSSGDD552 pKa = 3.75DD553 pKa = 3.17TLLGFDD559 pKa = 5.23GNDD562 pKa = 3.21TLAGDD567 pKa = 4.65DD568 pKa = 4.8GRR570 pKa = 11.84DD571 pKa = 3.4HH572 pKa = 6.94LAGGGGHH579 pKa = 7.36DD580 pKa = 4.58EE581 pKa = 4.49LNGGAGDD588 pKa = 3.78DD589 pKa = 4.56WITGDD594 pKa = 4.41SGNDD598 pKa = 3.67TIEE601 pKa = 4.41GGAGADD607 pKa = 3.62TLIGGSGIDD616 pKa = 3.46TVNYY620 pKa = 8.74YY621 pKa = 10.38YY622 pKa = 11.07SSGGVTIDD630 pKa = 3.89LLTNAVSGGWAADD643 pKa = 3.48DD644 pKa = 4.35TISGFEE650 pKa = 3.66RR651 pKa = 11.84AYY653 pKa = 10.73GSNSGNDD660 pKa = 3.3VLKK663 pKa = 10.75GSNGANVLRR672 pKa = 11.84GYY674 pKa = 10.2GGNDD678 pKa = 2.5IVYY681 pKa = 10.5DD682 pKa = 4.0RR683 pKa = 11.84GGDD686 pKa = 3.74DD687 pKa = 4.68YY688 pKa = 12.23VDD690 pKa = 4.52LGDD693 pKa = 4.78GNDD696 pKa = 3.54AVIVGNGADD705 pKa = 3.73TLIGGSGIDD714 pKa = 3.46TVNYY718 pKa = 8.74YY719 pKa = 10.38YY720 pKa = 11.07SSGGVTIDD728 pKa = 3.89LLTNAVSGGWAADD741 pKa = 3.48DD742 pKa = 4.35TISGFEE748 pKa = 3.66RR749 pKa = 11.84AYY751 pKa = 10.73GSNSGNDD758 pKa = 3.3VLKK761 pKa = 10.75GSNGANVLRR770 pKa = 11.84GYY772 pKa = 10.2GGNDD776 pKa = 2.5IVYY779 pKa = 10.5DD780 pKa = 4.0RR781 pKa = 11.84GGDD784 pKa = 3.74DD785 pKa = 4.68YY786 pKa = 12.23VDD788 pKa = 4.52LGDD791 pKa = 4.78GNDD794 pKa = 3.54AVIVGNGADD803 pKa = 3.73TLIGGSGIDD812 pKa = 3.46TVNYY816 pKa = 8.74YY817 pKa = 10.38YY818 pKa = 11.07SSGGVTIDD826 pKa = 3.89LLTNAVSGGWAADD839 pKa = 3.48DD840 pKa = 4.35TISGFEE846 pKa = 3.66RR847 pKa = 11.84AYY849 pKa = 10.73GSNSGNDD856 pKa = 3.3VLKK859 pKa = 10.75GSNGANVLRR868 pKa = 11.84GYY870 pKa = 10.3GGNDD874 pKa = 3.09RR875 pKa = 11.84LDD877 pKa = 3.37GRR879 pKa = 11.84AGNDD883 pKa = 3.41YY884 pKa = 11.13LDD886 pKa = 4.51GGSGGDD892 pKa = 3.47TLTGGVGADD901 pKa = 2.81HH902 pKa = 6.97FVFHH906 pKa = 6.55KK907 pKa = 9.83TYY909 pKa = 10.45GADD912 pKa = 4.19LITDD916 pKa = 4.25FAYY919 pKa = 10.45GLDD922 pKa = 3.89EE923 pKa = 5.02LVLDD927 pKa = 4.16HH928 pKa = 6.67SLWGGGLTEE937 pKa = 4.36AQVISIYY944 pKa = 10.95ASVIGEE950 pKa = 4.07NLVLDD955 pKa = 4.46FGGGDD960 pKa = 3.86TITLQGIASPGLLVDD975 pKa = 6.14DD976 pKa = 4.52ILIVV980 pKa = 3.44

Molecular weight: 103.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R8B1Q6|A0A2R8B1Q6_9RHOB Uncharacterized protein OS=Defluviimonas aquaemixtae OX=1542388 GN=DEA8626_00081 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.56GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4129

0

4129

1262290

30

2386

305.7

33.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.849 ± 0.055

0.906 ± 0.013

5.924 ± 0.031

6.182 ± 0.042

3.716 ± 0.024

8.908 ± 0.038

2.062 ± 0.02

5.081 ± 0.029

3.038 ± 0.036

9.909 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.627 ± 0.018

2.412 ± 0.023

5.218 ± 0.027

2.678 ± 0.023

7.269 ± 0.041

4.999 ± 0.025

5.259 ± 0.029

7.327 ± 0.03

1.449 ± 0.016

2.188 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski