Micromonospora craterilacus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6519 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2W2EIN9|A0A2W2EIN9_9ACTN DUF2154 domain-containing protein (Fragment) OS=Micromonospora craterilacus OX=1655439 GN=C1I95_29300 PE=4 SV=1
MM1 pKa = 6.87VTTTYY6 pKa = 10.36QVQGMTCGHH15 pKa = 6.44CVSAVSAEE23 pKa = 4.04VGAIPGVNEE32 pKa = 4.01VQVDD36 pKa = 4.06LAAGRR41 pKa = 11.84VTVNSDD47 pKa = 3.34QPLDD51 pKa = 3.89DD52 pKa = 4.07AAVRR56 pKa = 11.84AAVDD60 pKa = 3.24EE61 pKa = 4.64AGYY64 pKa = 10.41EE65 pKa = 3.93LAGAA69 pKa = 4.6

Molecular weight: 6.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2W2D3E8|A0A2W2D3E8_9ACTN Mg-protoporphyrin IX chelatase (Fragment) OS=Micromonospora craterilacus OX=1655439 GN=C1I95_32120 PE=3 SV=1
MM1 pKa = 7.77ASGRR5 pKa = 11.84GHH7 pKa = 5.86GRR9 pKa = 11.84GRR11 pKa = 11.84GHH13 pKa = 6.58GRR15 pKa = 11.84GHH17 pKa = 5.6GRR19 pKa = 11.84GRR21 pKa = 11.84GRR23 pKa = 11.84GRR25 pKa = 11.84GHH27 pKa = 6.33GRR29 pKa = 11.84GHH31 pKa = 6.28GRR33 pKa = 11.84GHH35 pKa = 6.28GRR37 pKa = 11.84GHH39 pKa = 6.28GRR41 pKa = 11.84GHH43 pKa = 6.28GRR45 pKa = 11.84GHH47 pKa = 6.49GRR49 pKa = 11.84GHH51 pKa = 5.92GHH53 pKa = 5.54GHH55 pKa = 5.19GHH57 pKa = 5.64GHH59 pKa = 6.73

Molecular weight: 6.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6519

0

6519

2067831

28

3900

317.2

33.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.884 ± 0.041

0.754 ± 0.009

6.021 ± 0.023

5.007 ± 0.031

2.666 ± 0.017

9.32 ± 0.027

2.153 ± 0.015

3.265 ± 0.021

1.62 ± 0.02

10.512 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.625 ± 0.011

1.915 ± 0.024

6.292 ± 0.024

2.866 ± 0.017

8.426 ± 0.036

4.706 ± 0.024

6.203 ± 0.033

8.997 ± 0.03

1.648 ± 0.015

2.119 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski