Micromonospora craterilacus
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6519 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2W2EIN9|A0A2W2EIN9_9ACTN DUF2154 domain-containing protein (Fragment) OS=Micromonospora craterilacus OX=1655439 GN=C1I95_29300 PE=4 SV=1
MM1 pKa = 6.87 VTTTYY6 pKa = 10.36 QVQGMTCGHH15 pKa = 6.44 CVSAVSAEE23 pKa = 4.04 VGAIPGVNEE32 pKa = 4.01 VQVDD36 pKa = 4.06 LAAGRR41 pKa = 11.84 VTVNSDD47 pKa = 3.34 QPLDD51 pKa = 3.89 DD52 pKa = 4.07 AAVRR56 pKa = 11.84 AAVDD60 pKa = 3.24 EE61 pKa = 4.64 AGYY64 pKa = 10.41 EE65 pKa = 3.93 LAGAA69 pKa = 4.6
Molecular weight: 6.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.797
IPC2_protein 3.961
IPC_protein 3.808
Toseland 3.617
ProMoST 3.999
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.656
Grimsley 3.541
Solomon 3.77
Lehninger 3.719
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.706
EMBOSS 3.77
Sillero 3.935
Patrickios 0.604
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.825
Protein with the highest isoelectric point:
>tr|A0A2W2D3E8|A0A2W2D3E8_9ACTN Mg-protoporphyrin IX chelatase (Fragment) OS=Micromonospora craterilacus OX=1655439 GN=C1I95_32120 PE=3 SV=1
MM1 pKa = 7.77 ASGRR5 pKa = 11.84 GHH7 pKa = 5.86 GRR9 pKa = 11.84 GRR11 pKa = 11.84 GHH13 pKa = 6.58 GRR15 pKa = 11.84 GHH17 pKa = 5.6 GRR19 pKa = 11.84 GRR21 pKa = 11.84 GRR23 pKa = 11.84 GRR25 pKa = 11.84 GHH27 pKa = 6.33 GRR29 pKa = 11.84 GHH31 pKa = 6.28 GRR33 pKa = 11.84 GHH35 pKa = 6.28 GRR37 pKa = 11.84 GHH39 pKa = 6.28 GRR41 pKa = 11.84 GHH43 pKa = 6.28 GRR45 pKa = 11.84 GHH47 pKa = 6.49 GRR49 pKa = 11.84 GHH51 pKa = 5.92 GHH53 pKa = 5.54 GHH55 pKa = 5.19 GHH57 pKa = 5.64 GHH59 pKa = 6.73
Molecular weight: 6.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.33
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.618
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.34
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.277
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6519
0
6519
2067831
28
3900
317.2
33.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.884 ± 0.041
0.754 ± 0.009
6.021 ± 0.023
5.007 ± 0.031
2.666 ± 0.017
9.32 ± 0.027
2.153 ± 0.015
3.265 ± 0.021
1.62 ± 0.02
10.512 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.625 ± 0.011
1.915 ± 0.024
6.292 ± 0.024
2.866 ± 0.017
8.426 ± 0.036
4.706 ± 0.024
6.203 ± 0.033
8.997 ± 0.03
1.648 ± 0.015
2.119 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here