Citricoccus sp. SGAir0253 
Average proteome isoelectric point is 6.16 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 2944 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A4P9JAH5|A0A4P9JAH5_9MICC Glycosyltransferase family 9 protein OS=Citricoccus sp. SGAir0253 OX=2567881 GN=E7744_07695 PE=4 SV=1MM1 pKa = 6.76  KK2 pKa = 9.18  TARR5 pKa = 11.84  LEE7 pKa = 4.36  SPAVRR12 pKa = 11.84  AAALAAVTVPFLAACGGGADD32 pKa = 4.11  GGEE35 pKa = 4.27  TLTFAAIPAEE45 pKa = 4.26  SSASLEE51 pKa = 3.91  SDD53 pKa = 3.52  YY54 pKa = 12.01  SNITALIEE62 pKa = 4.01  QEE64 pKa = 4.08  TGVDD68 pKa = 3.61  VEE70 pKa = 4.49  FQNASDD76 pKa = 3.83  YY77 pKa = 11.44  AAVIEE82 pKa = 4.37  GQRR85 pKa = 11.84  AGQIDD90 pKa = 3.76  IASYY94 pKa = 11.09  GPFSYY99 pKa = 10.89  VIAKK103 pKa = 10.39  DD104 pKa = 3.17  SGVNIEE110 pKa = 4.6  PVAAPTSDD118 pKa = 3.36  QDD120 pKa = 3.49  KK121 pKa = 10.48  QPAYY125 pKa = 9.64  TSLAYY130 pKa = 10.42  VRR132 pKa = 11.84  ADD134 pKa = 4.2  SDD136 pKa = 3.69  IQDD139 pKa = 3.99  LADD142 pKa = 3.95  LEE144 pKa = 4.55  GKK146 pKa = 9.57  KK147 pKa = 10.26  VCFVDD152 pKa = 3.61  AASTSGYY159 pKa = 9.41  LVPMKK164 pKa = 10.72  GLLDD168 pKa = 3.76  EE169 pKa = 6.2  GIDD172 pKa = 4.07  LNTEE176 pKa = 3.99  TEE178 pKa = 3.91  AVLAGGHH185 pKa = 6.27  DD186 pKa = 4.03  ASLLSLDD193 pKa = 5.23  AGSCDD198 pKa = 3.49  AAFAHH203 pKa = 6.43  DD204 pKa = 3.9  TMLNTLVTSGQVDD217 pKa = 3.9  EE218 pKa = 5.44  GALKK222 pKa = 9.93  PVWEE226 pKa = 4.48  SAPITEE232 pKa = 5.64  DD233 pKa = 4.27  PIALNMDD240 pKa = 4.26  TIEE243 pKa = 4.94  PEE245 pKa = 3.86  LAEE248 pKa = 4.27  QISTVIRR255 pKa = 11.84  EE256 pKa = 4.31  KK257 pKa = 10.98  ANKK260 pKa = 8.25  PALVEE265 pKa = 4.11  AGICASEE272 pKa = 4.22  EE273 pKa = 4.12  DD274 pKa = 4.04  CVLPEE279 pKa = 4.38  EE280 pKa = 4.56  IEE282 pKa = 4.16  YY283 pKa = 10.43  GYY285 pKa = 11.23  LPVDD289 pKa = 4.5  DD290 pKa = 5.65  SDD292 pKa = 3.77  FDD294 pKa = 4.84  AIRR297 pKa = 11.84  EE298 pKa = 4.05  ICAATDD304 pKa = 3.09  ADD306 pKa = 3.9  ACHH309 pKa = 6.61  SVGG312 pKa = 4.61  
 32.49 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.737 
IPC2_protein 3.77 
IPC_protein 3.783 
Toseland    3.567 
ProMoST     3.923 
Dawson      3.757 
Bjellqvist  3.91 
Wikipedia   3.668 
Rodwell     3.605 
Grimsley    3.478 
Solomon     3.757 
Lehninger   3.706 
Nozaki      3.872 
DTASelect   4.075 
Thurlkill   3.605 
EMBOSS      3.681 
Sillero     3.897 
Patrickios  1.863 
IPC_peptide 3.757 
IPC2_peptide  3.872 
IPC2.peptide.svr19  3.813 
 Protein with the highest isoelectric point: 
>tr|A0A4P9JBT8|A0A4P9JBT8_9MICC ABC transporter substrate-binding protein OS=Citricoccus sp. SGAir0253 OX=2567881 GN=E7744_09900 PE=4 SV=1MM1 pKa = 7.41  RR2 pKa = 11.84  WARR5 pKa = 11.84  PSSSAPRR12 pKa = 11.84  TPSPAPAGPASRR24 pKa = 11.84  SPTPARR30 pKa = 11.84  TARR33 pKa = 11.84  RR34 pKa = 11.84  RR35 pKa = 11.84  RR36 pKa = 11.84  PRR38 pKa = 11.84  RR39 pKa = 11.84  RR40 pKa = 11.84  GRR42 pKa = 11.84  RR43 pKa = 11.84  RR44 pKa = 11.84  PVRR47 pKa = 11.84  PRR49 pKa = 11.84  PAPTVRR55 pKa = 11.84  RR56 pKa = 11.84  GAARR60 pKa = 11.84  GGG62 pKa = 3.48  
 6.84 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.496 
IPC2_protein 11.506 
IPC_protein 13.115 
Toseland    13.276 
ProMoST     13.773 
Dawson      13.276 
Bjellqvist  13.276 
Wikipedia   13.759 
Rodwell     12.778 
Grimsley    13.32 
Solomon     13.773 
Lehninger   13.685 
Nozaki      13.276 
DTASelect   13.276 
Thurlkill   13.276 
EMBOSS      13.773 
Sillero     13.276 
Patrickios  12.501 
IPC_peptide 13.788 
IPC2_peptide  12.778 
IPC2.peptide.svr19  9.398 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        2944 
0
2944 
1013340
32
2154
344.2
36.65
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        14.018 ± 0.072
0.594 ± 0.011
5.842 ± 0.042
5.979 ± 0.042
2.739 ± 0.029
9.903 ± 0.047
2.256 ± 0.021
3.27 ± 0.031
1.605 ± 0.032
10.113 ± 0.054
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        1.881 ± 0.02
1.701 ± 0.022
6.178 ± 0.04
2.793 ± 0.028
7.923 ± 0.054
4.92 ± 0.031
5.971 ± 0.033
8.895 ± 0.046
1.509 ± 0.02
1.913 ± 0.022
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here