Citricoccus sp. SGAir0253
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2944 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P9JAH5|A0A4P9JAH5_9MICC Glycosyltransferase family 9 protein OS=Citricoccus sp. SGAir0253 OX=2567881 GN=E7744_07695 PE=4 SV=1
MM1 pKa = 6.76 KK2 pKa = 9.18 TARR5 pKa = 11.84 LEE7 pKa = 4.36 SPAVRR12 pKa = 11.84 AAALAAVTVPFLAACGGGADD32 pKa = 4.11 GGEE35 pKa = 4.27 TLTFAAIPAEE45 pKa = 4.26 SSASLEE51 pKa = 3.91 SDD53 pKa = 3.52 YY54 pKa = 12.01 SNITALIEE62 pKa = 4.01 QEE64 pKa = 4.08 TGVDD68 pKa = 3.61 VEE70 pKa = 4.49 FQNASDD76 pKa = 3.83 YY77 pKa = 11.44 AAVIEE82 pKa = 4.37 GQRR85 pKa = 11.84 AGQIDD90 pKa = 3.76 IASYY94 pKa = 11.09 GPFSYY99 pKa = 10.89 VIAKK103 pKa = 10.39 DD104 pKa = 3.17 SGVNIEE110 pKa = 4.6 PVAAPTSDD118 pKa = 3.36 QDD120 pKa = 3.49 KK121 pKa = 10.48 QPAYY125 pKa = 9.64 TSLAYY130 pKa = 10.42 VRR132 pKa = 11.84 ADD134 pKa = 4.2 SDD136 pKa = 3.69 IQDD139 pKa = 3.99 LADD142 pKa = 3.95 LEE144 pKa = 4.55 GKK146 pKa = 9.57 KK147 pKa = 10.26 VCFVDD152 pKa = 3.61 AASTSGYY159 pKa = 9.41 LVPMKK164 pKa = 10.72 GLLDD168 pKa = 3.76 EE169 pKa = 6.2 GIDD172 pKa = 4.07 LNTEE176 pKa = 3.99 TEE178 pKa = 3.91 AVLAGGHH185 pKa = 6.27 DD186 pKa = 4.03 ASLLSLDD193 pKa = 5.23 AGSCDD198 pKa = 3.49 AAFAHH203 pKa = 6.43 DD204 pKa = 3.9 TMLNTLVTSGQVDD217 pKa = 3.9 EE218 pKa = 5.44 GALKK222 pKa = 9.93 PVWEE226 pKa = 4.48 SAPITEE232 pKa = 5.64 DD233 pKa = 4.27 PIALNMDD240 pKa = 4.26 TIEE243 pKa = 4.94 PEE245 pKa = 3.86 LAEE248 pKa = 4.27 QISTVIRR255 pKa = 11.84 EE256 pKa = 4.31 KK257 pKa = 10.98 ANKK260 pKa = 8.25 PALVEE265 pKa = 4.11 AGICASEE272 pKa = 4.22 EE273 pKa = 4.12 DD274 pKa = 4.04 CVLPEE279 pKa = 4.38 EE280 pKa = 4.56 IEE282 pKa = 4.16 YY283 pKa = 10.43 GYY285 pKa = 11.23 LPVDD289 pKa = 4.5 DD290 pKa = 5.65 SDD292 pKa = 3.77 FDD294 pKa = 4.84 AIRR297 pKa = 11.84 EE298 pKa = 4.05 ICAATDD304 pKa = 3.09 ADD306 pKa = 3.9 ACHH309 pKa = 6.61 SVGG312 pKa = 4.61
Molecular weight: 32.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.77
IPC_protein 3.783
Toseland 3.567
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.706
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.605
EMBOSS 3.681
Sillero 3.897
Patrickios 1.863
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.813
Protein with the highest isoelectric point:
>tr|A0A4P9JBT8|A0A4P9JBT8_9MICC ABC transporter substrate-binding protein OS=Citricoccus sp. SGAir0253 OX=2567881 GN=E7744_09900 PE=4 SV=1
MM1 pKa = 7.41 RR2 pKa = 11.84 WARR5 pKa = 11.84 PSSSAPRR12 pKa = 11.84 TPSPAPAGPASRR24 pKa = 11.84 SPTPARR30 pKa = 11.84 TARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 PRR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 GRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 PVRR47 pKa = 11.84 PRR49 pKa = 11.84 PAPTVRR55 pKa = 11.84 RR56 pKa = 11.84 GAARR60 pKa = 11.84 GGG62 pKa = 3.48
Molecular weight: 6.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.496
IPC2_protein 11.506
IPC_protein 13.115
Toseland 13.276
ProMoST 13.773
Dawson 13.276
Bjellqvist 13.276
Wikipedia 13.759
Rodwell 12.778
Grimsley 13.32
Solomon 13.773
Lehninger 13.685
Nozaki 13.276
DTASelect 13.276
Thurlkill 13.276
EMBOSS 13.773
Sillero 13.276
Patrickios 12.501
IPC_peptide 13.788
IPC2_peptide 12.778
IPC2.peptide.svr19 9.398
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2944
0
2944
1013340
32
2154
344.2
36.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.018 ± 0.072
0.594 ± 0.011
5.842 ± 0.042
5.979 ± 0.042
2.739 ± 0.029
9.903 ± 0.047
2.256 ± 0.021
3.27 ± 0.031
1.605 ± 0.032
10.113 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.881 ± 0.02
1.701 ± 0.022
6.178 ± 0.04
2.793 ± 0.028
7.923 ± 0.054
4.92 ± 0.031
5.971 ± 0.033
8.895 ± 0.046
1.509 ± 0.02
1.913 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here