Candidatus Nesciobacter abundans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales; Holosporaceae; Candidatus Nesciobacter

Average proteome isoelectric point is 7.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 501 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A5C0UFQ8|A0A5C0UFQ8_9PROT 50S ribosomal protein L17 OS=Candidatus Nesciobacter abundans OX=2601668 GN=rplQ PE=3 SV=1
MM1 pKa = 7.61CEE3 pKa = 4.0KK4 pKa = 10.46KK5 pKa = 10.24SVEE8 pKa = 4.14IIFVYY13 pKa = 10.43PDD15 pKa = 3.43GSKK18 pKa = 10.29KK19 pKa = 10.21VCNANVGDD27 pKa = 4.67NMLQVIRR34 pKa = 11.84KK35 pKa = 7.37NHH37 pKa = 5.6IPMACSCGGALACGSCHH54 pKa = 5.94GTLDD58 pKa = 3.55QPFFDD63 pKa = 5.41KK64 pKa = 11.13IEE66 pKa = 5.07DD67 pKa = 3.46SDD69 pKa = 3.7QAMSEE74 pKa = 4.34TEE76 pKa = 4.33EE77 pKa = 3.76EE78 pKa = 4.16TMEE81 pKa = 4.45YY82 pKa = 10.28FVNDD86 pKa = 3.96DD87 pKa = 3.46ATDD90 pKa = 3.66TSRR93 pKa = 11.84LCCQVDD99 pKa = 3.55VRR101 pKa = 11.84EE102 pKa = 4.64EE103 pKa = 3.99LNGMHH108 pKa = 7.24IYY110 pKa = 10.05IPKK113 pKa = 8.08NTSDD117 pKa = 3.13

Molecular weight:
13.02 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A5C0UI69|A0A5C0UI69_9PROT ABC transporter ATP-binding protein OS=Candidatus Nesciobacter abundans OX=2601668 GN=FZC36_01365 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 8.91VLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.99GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight:
5.17 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

501

0

501

174585

41

2723

348.5

39.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.444 ± 0.083

1.376 ± 0.039

5.299 ± 0.084

7.196 ± 0.125

5.331 ± 0.118

5.4 ± 0.105

1.769 ± 0.041

8.838 ± 0.097

10.186 ± 0.101

8.911 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.485 ± 0.049

6.649 ± 0.089

2.73 ± 0.053

2.599 ± 0.053

3.485 ± 0.078

9.239 ± 0.106

4.148 ± 0.059

5.626 ± 0.087

0.805 ± 0.03

3.484 ± 0.067

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski