Candidatus Nesciobacter abundans
Average proteome isoelectric point is 7.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 501 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C0UFQ8|A0A5C0UFQ8_9PROT 50S ribosomal protein L17 OS=Candidatus Nesciobacter abundans OX=2601668 GN=rplQ PE=3 SV=1
MM1 pKa = 7.61 CEE3 pKa = 4.0 KK4 pKa = 10.46 KK5 pKa = 10.24 SVEE8 pKa = 4.14 IIFVYY13 pKa = 10.43 PDD15 pKa = 3.43 GSKK18 pKa = 10.29 KK19 pKa = 10.21 VCNANVGDD27 pKa = 4.67 NMLQVIRR34 pKa = 11.84 KK35 pKa = 7.37 NHH37 pKa = 5.6 IPMACSCGGALACGSCHH54 pKa = 5.94 GTLDD58 pKa = 3.55 QPFFDD63 pKa = 5.41 KK64 pKa = 11.13 IEE66 pKa = 5.07 DD67 pKa = 3.46 SDD69 pKa = 3.7 QAMSEE74 pKa = 4.34 TEE76 pKa = 4.33 EE77 pKa = 3.76 EE78 pKa = 4.16 TMEE81 pKa = 4.45 YY82 pKa = 10.28 FVNDD86 pKa = 3.96 DD87 pKa = 3.46 ATDD90 pKa = 3.66 TSRR93 pKa = 11.84 LCCQVDD99 pKa = 3.55 VRR101 pKa = 11.84 EE102 pKa = 4.64 EE103 pKa = 3.99 LNGMHH108 pKa = 7.24 IYY110 pKa = 10.05 IPKK113 pKa = 8.08 NTSDD117 pKa = 3.13
Molecular weight: 13.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.122
IPC2_protein 4.431
IPC_protein 4.342
Toseland 4.177
ProMoST 4.406
Dawson 4.317
Bjellqvist 4.507
Wikipedia 4.215
Rodwell 4.19
Grimsley 4.088
Solomon 4.304
Lehninger 4.266
Nozaki 4.431
DTASelect 4.609
Thurlkill 4.202
EMBOSS 4.228
Sillero 4.469
Patrickios 2.003
IPC_peptide 4.317
IPC2_peptide 4.457
IPC2.peptide.svr19 4.38
Protein with the highest isoelectric point:
>tr|A0A5C0UI69|A0A5C0UI69_9PROT ABC transporter ATP-binding protein OS=Candidatus Nesciobacter abundans OX=2601668 GN=FZC36_01365 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 8.96 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.62 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 8.91 VLSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.99 GRR39 pKa = 11.84 KK40 pKa = 8.67 VLSAA44 pKa = 4.05
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
501
0
501
174585
41
2723
348.5
39.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.444 ± 0.083
1.376 ± 0.039
5.299 ± 0.084
7.196 ± 0.125
5.331 ± 0.118
5.4 ± 0.105
1.769 ± 0.041
8.838 ± 0.097
10.186 ± 0.101
8.911 ± 0.081
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.485 ± 0.049
6.649 ± 0.089
2.73 ± 0.053
2.599 ± 0.053
3.485 ± 0.078
9.239 ± 0.106
4.148 ± 0.059
5.626 ± 0.087
0.805 ± 0.03
3.484 ± 0.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here