Streptomyces spongiae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9455 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N8XXS9|A0A5N8XXS9_9ACTN Thiosulfate sulfurtransferase OS=Streptomyces spongiae OX=565072 GN=FNH08_45600 PE=4 SV=1
MM1 pKa = 7.36TDD3 pKa = 2.6AWDD6 pKa = 3.91AFLDD10 pKa = 3.75ALTDD14 pKa = 3.72AGVSNPVDD22 pKa = 3.56ALSALAGSGVDD33 pKa = 5.36LDD35 pKa = 4.68ALTPAQVAEE44 pKa = 5.05IISEE48 pKa = 4.32LNGDD52 pKa = 4.1SQSSDD57 pKa = 2.74AGHH60 pKa = 6.97DD61 pKa = 3.42VRR63 pKa = 11.84FGKK66 pKa = 10.45GGYY69 pKa = 8.96HH70 pKa = 5.28GTYY73 pKa = 10.44NGIPNCWFSSDD84 pKa = 3.42GKK86 pKa = 11.13VYY88 pKa = 10.53AADD91 pKa = 4.14GTCVGAAA98 pKa = 3.55

Molecular weight: 9.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N8XIB1|A0A5N8XIB1_9ACTN Aromatic ring-hydroxylating dioxygenase subunit alpha OS=Streptomyces spongiae OX=565072 GN=FNH08_16520 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9455

0

9455

3197008

20

6282

338.1

36.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.021 ± 0.03

0.775 ± 0.008

6.117 ± 0.02

5.731 ± 0.025

2.781 ± 0.013

9.362 ± 0.023

2.331 ± 0.013

3.183 ± 0.017

2.231 ± 0.024

10.312 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.74 ± 0.008

1.865 ± 0.013

5.969 ± 0.019

2.786 ± 0.014

7.916 ± 0.031

5.305 ± 0.019

6.331 ± 0.019

8.538 ± 0.024

1.559 ± 0.011

2.146 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski