Staphylococcus virus PH15

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Rockefellervirus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A1BU75|A1BU75_9CAUD Uncharacterized protein OS=Staphylococcus virus PH15 OX=399185 GN=ph26 PE=4 SV=1
MM1 pKa = 7.79LINVLNLNDD10 pKa = 3.87SQDD13 pKa = 3.51GNRR16 pKa = 11.84IKK18 pKa = 10.81QGDD21 pKa = 4.44LSHH24 pKa = 6.11MRR26 pKa = 11.84YY27 pKa = 9.41ILSDD31 pKa = 3.66TNNDD35 pKa = 3.71DD36 pKa = 4.57LKK38 pKa = 11.48LDD40 pKa = 3.86GLPAKK45 pKa = 10.63VFLTDD50 pKa = 3.42STGVKK55 pKa = 10.05YY56 pKa = 10.56IYY58 pKa = 8.7DD59 pKa = 3.78TTVRR63 pKa = 11.84QYY65 pKa = 12.07DD66 pKa = 3.4NAYY69 pKa = 9.72VCDD72 pKa = 3.85VVINQIIPANTYY84 pKa = 10.19SLEE87 pKa = 3.64IWVDD91 pKa = 3.26NKK93 pKa = 11.02YY94 pKa = 10.97VFPSDD99 pKa = 3.32NKK101 pKa = 11.01AKK103 pKa = 10.17IQVTEE108 pKa = 4.2SVIGRR113 pKa = 11.84QLINTQNHH121 pKa = 6.33DD122 pKa = 3.2LWQEE126 pKa = 3.89MIEE129 pKa = 4.18YY130 pKa = 9.84GVKK133 pKa = 10.46NGLIKK138 pKa = 10.83NQTEE142 pKa = 4.13SEE144 pKa = 4.39EE145 pKa = 4.14NFVIGEE151 pKa = 4.09NAPTDD156 pKa = 3.66TTKK159 pKa = 10.29IWIDD163 pKa = 3.3TTGGNEE169 pKa = 3.62

Molecular weight: 19.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A1BU65|A1BU65_9CAUD Putative phage tape measure protein OS=Staphylococcus virus PH15 OX=399185 GN=ph16 PE=4 SV=1
MM1 pKa = 7.54SLTPNEE7 pKa = 3.89WKK9 pKa = 10.31DD10 pKa = 3.28WVVGRR15 pKa = 11.84RR16 pKa = 11.84LALLDD21 pKa = 3.78EE22 pKa = 4.42QEE24 pKa = 4.32TLLFGAQANGLVQAGKK40 pKa = 9.58SLKK43 pKa = 10.18RR44 pKa = 11.84LQKK47 pKa = 8.59QLEE50 pKa = 3.97RR51 pKa = 11.84ARR53 pKa = 11.84YY54 pKa = 6.86EE55 pKa = 3.64VRR57 pKa = 11.84GQSEE61 pKa = 4.38EE62 pKa = 4.06YY63 pKa = 10.73EE64 pKa = 4.03RR65 pKa = 11.84MKK67 pKa = 10.59EE68 pKa = 4.04RR69 pKa = 11.84KK70 pKa = 8.73LAHH73 pKa = 6.28NKK75 pKa = 10.13RR76 pKa = 11.84IRR78 pKa = 11.84NVQKK82 pKa = 9.93QGTRR86 pKa = 11.84RR87 pKa = 11.84FMNSLRR93 pKa = 11.84NTSQKK98 pKa = 11.01GGG100 pKa = 3.49

Molecular weight: 11.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

13558

40

1034

199.4

22.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.406 ± 0.453

0.575 ± 0.089

6.373 ± 0.361

7.479 ± 0.591

3.592 ± 0.219

5.945 ± 0.44

1.778 ± 0.165

7.236 ± 0.238

9.493 ± 0.429

7.663 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.611 ± 0.187

6.823 ± 0.341

2.818 ± 0.194

4.02 ± 0.245

4.625 ± 0.227

5.768 ± 0.291

5.996 ± 0.225

6.048 ± 0.261

1.335 ± 0.144

4.418 ± 0.392
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski