Belnapia rosea
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5712 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G6PRX9|A0A1G6PRX9_9PROT Disulfide bond formation protein DsbB OS=Belnapia rosea OX=938405 GN=SAMN04487779_1002495 PE=4 SV=1
MM1 pKa = 6.83 TAYY4 pKa = 10.26 HH5 pKa = 6.06 GTNASEE11 pKa = 4.3 TFAGSASADD20 pKa = 3.56 MIFGEE25 pKa = 4.9 GGNDD29 pKa = 3.24 SLFGNDD35 pKa = 4.91 GNDD38 pKa = 3.18 SLYY41 pKa = 11.14 GGTGADD47 pKa = 3.92 MLNGGAGDD55 pKa = 5.07 DD56 pKa = 4.56 IICGGIGNDD65 pKa = 3.55 VLTGGAGADD74 pKa = 2.94 IFRR77 pKa = 11.84 FTGVTSTSPGDD88 pKa = 3.48 SEE90 pKa = 6.18 GIGDD94 pKa = 4.78 VINDD98 pKa = 3.94 FQQGEE103 pKa = 4.21 DD104 pKa = 3.51 HH105 pKa = 7.12 IKK107 pKa = 9.27 FTGVSQRR114 pKa = 11.84 TVTMNLEE121 pKa = 4.08 ADD123 pKa = 3.71 GDD125 pKa = 4.35 LVIHH129 pKa = 6.37 YY130 pKa = 7.61 GTLGGAPGPNQGTITLKK147 pKa = 11.11 GLGQYY152 pKa = 10.93 LSGSDD157 pKa = 4.53 FIFTT161 pKa = 4.27
Molecular weight: 16.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.681
IPC_protein 3.681
Toseland 3.452
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.681
Rodwell 3.516
Grimsley 3.376
Solomon 3.681
Lehninger 3.643
Nozaki 3.821
DTASelect 4.101
Thurlkill 3.541
EMBOSS 3.681
Sillero 3.821
Patrickios 1.875
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.736
Protein with the highest isoelectric point:
>tr|A0A1G6SHV9|A0A1G6SHV9_9PROT Cytoskeleton protein RodZ OS=Belnapia rosea OX=938405 GN=SAMN04487779_100512 PE=4 SV=1
MM1 pKa = 7.38 HH2 pKa = 7.58 AFLAWALGLIGGAFFSIGLGGMWAQVVAALRR33 pKa = 11.84 SPAFGGYY40 pKa = 9.38 SATGWLVPTLLLALAAAAWAGAALIWAARR69 pKa = 11.84 PRR71 pKa = 11.84 PRR73 pKa = 11.84 AILPRR78 pKa = 11.84 HH79 pKa = 6.48 LGRR82 pKa = 11.84 GTAFRR87 pKa = 11.84 RR88 pKa = 11.84 RR89 pKa = 11.84 RR90 pKa = 3.43
Molecular weight: 9.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.394
IPC2_protein 11.067
IPC_protein 12.442
Toseland 12.603
ProMoST 13.115
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.857
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.147
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5712
0
5712
1791141
24
2426
313.6
33.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.709 ± 0.063
0.876 ± 0.009
4.895 ± 0.027
5.728 ± 0.029
3.197 ± 0.021
9.472 ± 0.033
2.003 ± 0.016
4.105 ± 0.021
1.904 ± 0.025
11.101 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.371 ± 0.014
1.99 ± 0.018
6.342 ± 0.031
3.079 ± 0.02
8.481 ± 0.037
4.387 ± 0.022
4.904 ± 0.024
7.132 ± 0.025
1.527 ± 0.013
1.797 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here