Proteome-pI 2.0 - Proteome Isoelectric Point Database

Mannheimia phage vB_MhS_587AP2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A0M3LQ61|A0A0M3LQ61_9CAUD Host specificity protein J OS=Mannheimia phage vB_MhS_587AP2 OX=1572745 GN=587AP2_27 PE=4 SV=1

MM1 pKa = 7.59IGYY4 pKa = 9.72EE5 pKa = 4.37DD6 pKa = 3.76YY7 pKa = 11.1TDD9 pKa = 5.21ASLQPEE15 pKa = 4.28EE16 pKa = 5.21DD17 pKa = 3.68PSEE20 pKa = 4.01EE21 pKa = 3.84AFLLVEE27 pKa = 4.21EE28 pKa = 5.12KK29 pKa = 10.53IIDD32 pKa = 3.64VLEE35 pKa = 4.37NNGFEE40 pKa = 4.34TVATILAGSDD50 pKa = 3.44GWCNAINAYY59 pKa = 9.94LAEE62 pKa = 4.32QNKK65 pKa = 8.83HH66 pKa = 5.6RR67 pKa = 11.84NEE69 pKa = 4.01FF70 pKa = 3.52

Molecular weight: 7.88 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A0M3LPY7|A0A0M3LPY7_9CAUD Uncharacterized protein OS=Mannheimia phage vB_MhS_587AP2 OX=1572745 GN=587AP2_58 PE=4 SV=1

MM1 pKa = 7.11KK2 pKa = 9.5TFSIHH7 pKa = 4.92TSVWHH12 pKa = 6.96DD13 pKa = 3.34SSTKK17 pKa = 10.26GFRR20 pKa = 11.84HH21 pKa = 5.78NIKK24 pKa = 10.21HH25 pKa = 6.0KK26 pKa = 10.89RR27 pKa = 11.84HH28 pKa = 6.1DD29 pKa = 3.41CWRR32 pKa = 11.84ADD34 pKa = 3.03IRR36 pKa = 11.84IKK38 pKa = 10.89QKK40 pKa = 10.65INNVWVQISRR50 pKa = 11.84IRR52 pKa = 11.84KK53 pKa = 8.58RR54 pKa = 11.84FKK56 pKa = 10.71CPEE59 pKa = 3.81QAKK62 pKa = 10.53SFAEE66 pKa = 3.77QWKK69 pKa = 7.98QTEE72 pKa = 4.42TPAFLQHH79 pKa = 6.11QINRR83 pKa = 11.84QALRR87 pKa = 11.84EE88 pKa = 3.95EE89 pKa = 4.41LSNLEE94 pKa = 3.55QRR96 pKa = 11.84KK97 pKa = 7.89IQKK100 pKa = 10.24NGMLEE105 pKa = 4.07VIEE108 pKa = 4.16YY109 pKa = 10.11RR110 pKa = 11.84ISQIKK115 pKa = 9.71EE116 pKa = 3.59LLKK119 pKa = 10.99

Molecular weight: 14.48 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
77	0	77	14768	37	1954	191.8	21.6

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
8.092 ± 0.549	1.043 ± 0.13	5.282 ± 0.213	6.988 ± 0.336	4.076 ± 0.242	6.291 ± 0.239	1.706 ± 0.179	6.927 ± 0.199	7.205 ± 0.299	8.166 ± 0.301

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.187 ± 0.237	5.966 ± 0.354	3.298 ± 0.268	4.93 ± 0.333	4.625 ± 0.21	6.358 ± 0.315	5.695 ± 0.447	6.155 ± 0.265	1.503 ± 0.091	3.508 ± 0.179

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski