Microvirga subterranea

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Microvirga

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4897 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A370HZ70|A0A370HZ70_9RHIZ Uncharacterized protein OS=Microvirga subterranea OX=186651 GN=DES45_101512 PE=4 SV=1
MM1 pKa = 7.82AFFDD5 pKa = 3.62VTNAFGQGFNMSATGDD21 pKa = 3.85SGWSFVEE28 pKa = 3.62ADD30 pKa = 3.96PNVSEE35 pKa = 5.02QLYY38 pKa = 10.75SDD40 pKa = 4.17DD41 pKa = 4.09GKK43 pKa = 11.41LITFSVNGSPYY54 pKa = 9.22INFYY58 pKa = 10.9GFTYY62 pKa = 9.81TLGYY66 pKa = 8.76TYY68 pKa = 10.53PDD70 pKa = 3.46GNRR73 pKa = 11.84DD74 pKa = 3.63VLIDD78 pKa = 4.25NIWYY82 pKa = 9.66YY83 pKa = 11.91NNGQNVMSVTQLNLQTTIFDD103 pKa = 3.93LQGNAWSVRR112 pKa = 11.84LNNGNDD118 pKa = 3.34TFDD121 pKa = 3.87GNDD124 pKa = 3.37YY125 pKa = 11.1NDD127 pKa = 3.78VIRR130 pKa = 11.84GGFGNDD136 pKa = 4.41LIIGYY141 pKa = 10.06AGDD144 pKa = 5.64DD145 pKa = 3.74ILLGDD150 pKa = 4.56QGNDD154 pKa = 3.56TIGGGAGNDD163 pKa = 4.17LIFGGDD169 pKa = 3.47GYY171 pKa = 10.67DD172 pKa = 3.24TVSYY176 pKa = 11.2GGFSTNYY183 pKa = 8.76IFTRR187 pKa = 11.84NVDD190 pKa = 3.38GSVTVTDD197 pKa = 3.87TTGGWGTDD205 pKa = 2.95TVYY208 pKa = 10.92DD209 pKa = 3.64VEE211 pKa = 5.43AFYY214 pKa = 11.04FNNGTFSLSSLLPIDD229 pKa = 4.79PPAPPPPPPPPPPTPEE245 pKa = 3.98EE246 pKa = 4.15IYY248 pKa = 10.86AGEE251 pKa = 4.09DD252 pKa = 3.19TLYY255 pKa = 9.54GTSGSNTLKK264 pKa = 10.87GYY266 pKa = 10.26GGNDD270 pKa = 3.41TLRR273 pKa = 11.84GQGGNDD279 pKa = 3.3YY280 pKa = 11.23LFGGDD285 pKa = 4.42GNDD288 pKa = 3.26VLYY291 pKa = 11.21GGAGKK296 pKa = 10.08DD297 pKa = 3.08AFVFDD302 pKa = 4.22TKK304 pKa = 10.88PNKK307 pKa = 7.57TTNKK311 pKa = 10.26DD312 pKa = 3.29AIKK315 pKa = 10.33DD316 pKa = 3.87FKK318 pKa = 11.56VVDD321 pKa = 3.34DD322 pKa = 5.12TIRR325 pKa = 11.84LDD327 pKa = 3.33NAVFTKK333 pKa = 10.82VGGNGTLKK341 pKa = 10.91ASAFWTNTTGKK352 pKa = 10.1AHH354 pKa = 7.21DD355 pKa = 3.83KK356 pKa = 10.21DD357 pKa = 3.99DD358 pKa = 3.73RR359 pKa = 11.84VIYY362 pKa = 10.75DD363 pKa = 3.29KK364 pKa = 11.41DD365 pKa = 3.89SGVLYY370 pKa = 10.78YY371 pKa = 10.79DD372 pKa = 4.17ADD374 pKa = 4.04GSGKK378 pKa = 9.86GAAVAFATISKK389 pKa = 10.18NLALTNKK396 pKa = 9.91DD397 pKa = 3.61FYY399 pKa = 11.0IVV401 pKa = 3.15

Molecular weight: 42.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A370HXV8|A0A370HXV8_9RHIZ Xanthine dehydrogenase YagT iron-sulfur-binding subunit OS=Microvirga subterranea OX=186651 GN=DES45_10129 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 10.27GLPKK6 pKa = 10.1GWRR9 pKa = 11.84IHH11 pKa = 7.18DD12 pKa = 3.67GRR14 pKa = 11.84DD15 pKa = 3.3PEE17 pKa = 4.25EE18 pKa = 4.07EE19 pKa = 3.81LRR21 pKa = 11.84KK22 pKa = 9.95LKK24 pKa = 10.63RR25 pKa = 11.84RR26 pKa = 11.84FQAVSDD32 pKa = 3.65RR33 pKa = 11.84FHH35 pKa = 6.84RR36 pKa = 11.84ARR38 pKa = 11.84RR39 pKa = 11.84LRR41 pKa = 11.84SLLRR45 pKa = 11.84QIRR48 pKa = 11.84LPALLLTGIFAVVTVLVTLSPWPFTTTVRR77 pKa = 11.84HH78 pKa = 5.61LASFPSCGLARR89 pKa = 11.84AIGLAPAYY97 pKa = 10.3RR98 pKa = 11.84GDD100 pKa = 3.63PGYY103 pKa = 9.44WAHH106 pKa = 6.7QDD108 pKa = 3.2EE109 pKa = 5.05DD110 pKa = 4.29SDD112 pKa = 4.44GRR114 pKa = 11.84SCEE117 pKa = 3.92TWKK120 pKa = 10.69SHH122 pKa = 6.05

Molecular weight: 13.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4897

0

4897

1507765

25

2599

307.9

33.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.033 ± 0.049

0.758 ± 0.011

5.652 ± 0.036

5.818 ± 0.036

3.704 ± 0.023

8.662 ± 0.041

2.013 ± 0.017

5.111 ± 0.024

3.198 ± 0.029

10.196 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.356 ± 0.02

2.58 ± 0.029

5.342 ± 0.029

3.179 ± 0.022

7.385 ± 0.041

5.474 ± 0.024

5.333 ± 0.029

7.683 ± 0.029

1.309 ± 0.015

2.214 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski