Klebsiella phage ST13-OXA48phi12.4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482MG85|A0A482MG85_9CAUD Helix-turn-helix domain-containing protein OS=Klebsiella phage ST13-OXA48phi12.4 OX=2510467 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 10.01IKK4 pKa = 10.39CIKK7 pKa = 9.25DD8 pKa = 3.26TEE10 pKa = 4.81GYY12 pKa = 5.66WTEE15 pKa = 4.05GEE17 pKa = 4.32MYY19 pKa = 9.28PARR22 pKa = 11.84VVAGGFVQVGDD33 pKa = 4.47DD34 pKa = 4.37DD35 pKa = 5.04DD36 pKa = 5.41PNGEE40 pKa = 4.12GWSAAPMEE48 pKa = 3.99YY49 pKa = 10.01RR50 pKa = 11.84EE51 pKa = 5.16DD52 pKa = 3.71GSIVYY57 pKa = 9.02QVGGIEE63 pKa = 4.22GDD65 pKa = 3.91VLFEE69 pKa = 4.19EE70 pKa = 5.34ASHH73 pKa = 7.3DD74 pKa = 3.65

Molecular weight: 8.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482MFE0|A0A482MFE0_9CAUD Tail tape-measure protein 1 OS=Klebsiella phage ST13-OXA48phi12.4 OX=2510467 PE=4 SV=1
MM1 pKa = 7.61RR2 pKa = 11.84LINRR6 pKa = 11.84SRR8 pKa = 11.84HH9 pKa = 5.19SPLGRR14 pKa = 11.84QACDD18 pKa = 2.61AALAKK23 pKa = 10.28HH24 pKa = 5.58VEE26 pKa = 4.22LYY28 pKa = 11.08GDD30 pKa = 3.63YY31 pKa = 10.96GRR33 pKa = 11.84QKK35 pKa = 9.55MKK37 pKa = 9.47RR38 pKa = 11.84TYY40 pKa = 8.99TVVVQGTKK48 pKa = 8.73ITVEE52 pKa = 4.08VVNRR56 pKa = 11.84NCSYY60 pKa = 11.08VATAMNCARR69 pKa = 11.84RR70 pKa = 11.84LRR72 pKa = 11.84HH73 pKa = 6.46LPGQVSS79 pKa = 3.19

Molecular weight: 8.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

0

85

17652

30

1128

207.7

23.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.044 ± 0.427

1.184 ± 0.157

5.784 ± 0.23

6.271 ± 0.296

3.541 ± 0.195

7.166 ± 0.262

1.841 ± 0.15

5.433 ± 0.228

4.968 ± 0.288

9.002 ± 0.275

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.629 ± 0.1

3.864 ± 0.171

4.368 ± 0.239

4.43 ± 0.268

6.124 ± 0.238

6.368 ± 0.267

5.79 ± 0.244

6.56 ± 0.279

1.711 ± 0.127

2.923 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski