Marivita geojedonensis
Average proteome isoelectric point is 5.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3878 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X4NFF1|A0A1X4NFF1_9RHOB DNA-binding protein OS=Marivita geojedonensis OX=1123756 GN=MGEO_18150 PE=4 SV=1
MM1 pKa = 7.26 KK2 pKa = 10.23 HH3 pKa = 6.2 ILLGTALVATAATSTYY19 pKa = 10.79 AGSADD24 pKa = 3.96 LAPVEE29 pKa = 4.05 PMIVEE34 pKa = 4.52 PVTVAPTQMGGDD46 pKa = 3.2 WTGAYY51 pKa = 9.98 GGLSFGNLSADD62 pKa = 4.52 ADD64 pKa = 4.13 DD65 pKa = 5.89 LDD67 pKa = 5.2 DD68 pKa = 4.46 SEE70 pKa = 5.25 GVYY73 pKa = 10.53 GVYY76 pKa = 10.39 GGYY79 pKa = 10.48 DD80 pKa = 3.31 YY81 pKa = 11.67 DD82 pKa = 3.89 FGQFVVGGEE91 pKa = 4.12 LDD93 pKa = 3.76 YY94 pKa = 10.82 QTGEE98 pKa = 4.48 DD99 pKa = 3.47 IQLGGIEE106 pKa = 4.14 VDD108 pKa = 3.32 DD109 pKa = 3.89 VLRR112 pKa = 11.84 AKK114 pKa = 10.53 VRR116 pKa = 11.84 GGYY119 pKa = 10.08 DD120 pKa = 3.19 LGRR123 pKa = 11.84 TLVYY127 pKa = 9.36 GTVGAAQLNTNIGDD141 pKa = 3.58 DD142 pKa = 3.51 TGFVGGLGVEE152 pKa = 4.4 YY153 pKa = 10.42 KK154 pKa = 9.38 VTEE157 pKa = 4.14 QITVGGEE164 pKa = 3.9 YY165 pKa = 10.12 LAHH168 pKa = 7.12 RR169 pKa = 11.84 FDD171 pKa = 5.52 DD172 pKa = 4.2 FDD174 pKa = 3.68 GTGVDD179 pKa = 3.9 VEE181 pKa = 4.7 ADD183 pKa = 3.6 TVSIRR188 pKa = 11.84 GSFRR192 pKa = 11.84 FF193 pKa = 3.65
Molecular weight: 20.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.689
IPC2_protein 3.757
IPC_protein 3.77
Toseland 3.541
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.719
Rodwell 3.592
Grimsley 3.452
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.139
Thurlkill 3.605
EMBOSS 3.732
Sillero 3.897
Patrickios 1.074
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.78
Protein with the highest isoelectric point:
>tr|A0A1X4NPQ8|A0A1X4NPQ8_9RHOB Enoyl-CoA hydratase OS=Marivita geojedonensis OX=1123756 GN=MGEO_04625 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3878
0
3878
1194701
41
1964
308.1
33.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.664 ± 0.044
0.928 ± 0.014
6.199 ± 0.034
5.991 ± 0.034
3.866 ± 0.023
8.468 ± 0.036
2.069 ± 0.022
5.331 ± 0.027
3.311 ± 0.032
9.958 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.839 ± 0.018
2.685 ± 0.02
5.008 ± 0.023
3.188 ± 0.022
6.472 ± 0.042
5.348 ± 0.028
5.584 ± 0.026
7.369 ± 0.034
1.423 ± 0.016
2.298 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here