Salmonella phage SP01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Markadamsvirinae; Tequintavirus; Salmonella virus SP01

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 145 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A291NKZ2|A0A291NKZ2_9CAUD Uncharacterized protein OS=Salmonella phage SP01 OX=1920294 PE=4 SV=1
MM1 pKa = 6.89QVYY4 pKa = 10.36VLTRR8 pKa = 11.84DD9 pKa = 3.25INEE12 pKa = 3.91YY13 pKa = 9.95NQDD16 pKa = 2.51GEE18 pKa = 4.74YY19 pKa = 9.78FVKK22 pKa = 10.56VFAEE26 pKa = 4.49KK27 pKa = 10.07PSKK30 pKa = 10.21QQLLDD35 pKa = 3.63AGVPEE40 pKa = 4.86DD41 pKa = 3.5QAKK44 pKa = 10.79CILQDD49 pKa = 3.91KK50 pKa = 9.66EE51 pKa = 4.39FTGDD55 pKa = 3.25AYY57 pKa = 10.56EE58 pKa = 4.91CFYY61 pKa = 11.16LSCEE65 pKa = 4.28NII67 pKa = 3.48

Molecular weight: 7.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A291NKU6|A0A291NKU6_9CAUD PHB domain-containing protein OS=Salmonella phage SP01 OX=1920294 PE=4 SV=1
MM1 pKa = 7.79AKK3 pKa = 9.77QKK5 pKa = 10.18NAKK8 pKa = 7.58TQAAPAVKK16 pKa = 9.52TFPQTEE22 pKa = 4.31ANRR25 pKa = 11.84KK26 pKa = 8.9ARR28 pKa = 11.84LEE30 pKa = 3.84RR31 pKa = 11.84HH32 pKa = 5.4LRR34 pKa = 11.84KK35 pKa = 10.0HH36 pKa = 5.6PTDD39 pKa = 3.49AQAAQALNNPAPIRR53 pKa = 11.84QKK55 pKa = 10.71PKK57 pKa = 10.52AKK59 pKa = 9.68NATRR63 pKa = 11.84KK64 pKa = 8.39SYY66 pKa = 11.17KK67 pKa = 8.89MVMYY71 pKa = 10.31VEE73 pKa = 4.89NYY75 pKa = 7.4GHH77 pKa = 6.99KK78 pKa = 9.99SVPVSLSFNAGAEE91 pKa = 4.17LFSRR95 pKa = 11.84NGMSMKK101 pKa = 10.42DD102 pKa = 3.33YY103 pKa = 11.01EE104 pKa = 4.31KK105 pKa = 10.98AVNQKK110 pKa = 9.97RR111 pKa = 11.84KK112 pKa = 8.13PTADD116 pKa = 3.13VLRR119 pKa = 11.84DD120 pKa = 3.4TRR122 pKa = 11.84GQFGSVKK129 pKa = 9.73PNIFGVEE136 pKa = 3.85YY137 pKa = 11.02SKK139 pKa = 11.81DD140 pKa = 3.37NVRR143 pKa = 11.84ALCYY147 pKa = 10.2GVGIKK152 pKa = 8.51FTGDD156 pKa = 3.22SARR159 pKa = 11.84KK160 pKa = 7.85SAKK163 pKa = 9.24PARR166 pKa = 11.84KK167 pKa = 9.36RR168 pKa = 11.84KK169 pKa = 9.78AKK171 pKa = 10.37

Molecular weight: 19.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

145

0

145

29613

42

1226

204.2

22.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.547 ± 0.422

1.148 ± 0.104

6.041 ± 0.152

6.916 ± 0.234

4.029 ± 0.145

6.47 ± 0.219

1.915 ± 0.153

6.561 ± 0.174

7.412 ± 0.264

8.344 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.509 ± 0.132

5.46 ± 0.208

3.421 ± 0.153

3.792 ± 0.232

4.572 ± 0.154

6.588 ± 0.273

5.845 ± 0.236

6.278 ± 0.157

1.185 ± 0.087

3.964 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski