Amino acid dipepetide frequency for Salmonella phage SP01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.666AlaAla: 7.666 ± 1.49
0.675AlaCys: 0.675 ± 0.156
4.019AlaAsp: 4.019 ± 0.413
4.998AlaGlu: 4.998 ± 0.408
3.107AlaPhe: 3.107 ± 0.349
4.998AlaGly: 4.998 ± 0.427
1.655AlaHis: 1.655 ± 0.301
4.424AlaIle: 4.424 ± 0.427
6.518AlaLys: 6.518 ± 0.781
5.91AlaLeu: 5.91 ± 0.515
1.857AlaMet: 1.857 ± 0.284
3.58AlaAsn: 3.58 ± 0.436
2.229AlaPro: 2.229 ± 0.314
3.478AlaGln: 3.478 ± 0.378
3.208AlaArg: 3.208 ± 0.316
4.964AlaSer: 4.964 ± 1.012
4.221AlaThr: 4.221 ± 0.584
4.491AlaVal: 4.491 ± 0.45
0.878AlaTrp: 0.878 ± 0.18
2.6AlaTyr: 2.6 ± 0.294
0.0AlaXaa: 0.0 ± 0.0
Cys
0.743CysAla: 0.743 ± 0.167
0.203CysCys: 0.203 ± 0.082
0.608CysAsp: 0.608 ± 0.14
0.709CysGlu: 0.709 ± 0.132
0.405CysPhe: 0.405 ± 0.109
1.013CysGly: 1.013 ± 0.223
0.169CysHis: 0.169 ± 0.087
0.675CysIle: 0.675 ± 0.154
0.709CysLys: 0.709 ± 0.147
0.844CysLeu: 0.844 ± 0.154
0.304CysMet: 0.304 ± 0.092
0.642CysAsn: 0.642 ± 0.129
0.574CysPro: 0.574 ± 0.163
0.473CysGln: 0.473 ± 0.138
0.574CysArg: 0.574 ± 0.117
1.013CysSer: 1.013 ± 0.17
0.642CysThr: 0.642 ± 0.146
0.608CysVal: 0.608 ± 0.142
0.068CysTrp: 0.068 ± 0.044
0.507CysTyr: 0.507 ± 0.136
0.0CysXaa: 0.0 ± 0.0
Asp
4.458AspAla: 4.458 ± 0.465
0.709AspCys: 0.709 ± 0.176
2.769AspAsp: 2.769 ± 0.403
4.187AspGlu: 4.187 ± 0.412
2.87AspPhe: 2.87 ± 0.314
3.748AspGly: 3.748 ± 0.332
1.216AspHis: 1.216 ± 0.199
4.593AspIle: 4.593 ± 0.441
4.593AspLys: 4.593 ± 0.43
5.572AspLeu: 5.572 ± 0.487
1.925AspMet: 1.925 ± 0.266
2.668AspAsn: 2.668 ± 0.326
2.567AspPro: 2.567 ± 0.251
1.486AspGln: 1.486 ± 0.247
2.769AspArg: 2.769 ± 0.278
3.681AspSer: 3.681 ± 0.387
3.613AspThr: 3.613 ± 0.363
3.411AspVal: 3.411 ± 0.327
0.743AspTrp: 0.743 ± 0.152
2.837AspTyr: 2.837 ± 0.318
0.0AspXaa: 0.0 ± 0.0
Glu
6.079GluAla: 6.079 ± 0.438
0.844GluCys: 0.844 ± 0.206
3.276GluAsp: 3.276 ± 0.432
5.234GluGlu: 5.234 ± 0.535
2.465GluPhe: 2.465 ± 0.289
3.85GluGly: 3.85 ± 0.372
1.216GluHis: 1.216 ± 0.186
5.268GluIle: 5.268 ± 0.467
4.863GluLys: 4.863 ± 0.464
7.058GluLeu: 7.058 ± 0.476
2.195GluMet: 2.195 ± 0.325
2.6GluAsn: 2.6 ± 0.282
1.452GluPro: 1.452 ± 0.241
2.972GluGln: 2.972 ± 0.456
3.073GluArg: 3.073 ± 0.34
3.951GluSer: 3.951 ± 0.358
3.782GluThr: 3.782 ± 0.568
4.694GluVal: 4.694 ± 0.344
1.013GluTrp: 1.013 ± 0.189
3.242GluTyr: 3.242 ± 0.344
0.0GluXaa: 0.0 ± 0.0
Phe
2.465PheAla: 2.465 ± 0.294
0.236PheCys: 0.236 ± 0.099
2.837PheAsp: 2.837 ± 0.321
2.702PheGlu: 2.702 ± 0.293
1.52PhePhe: 1.52 ± 0.251
2.6PheGly: 2.6 ± 0.318
1.148PheHis: 1.148 ± 0.218
2.533PheIle: 2.533 ± 0.318
3.039PheLys: 3.039 ± 0.309
3.174PheLeu: 3.174 ± 0.307
0.709PheMet: 0.709 ± 0.125
2.668PheAsn: 2.668 ± 0.32
1.486PhePro: 1.486 ± 0.204
0.979PheGln: 0.979 ± 0.161
1.722PheArg: 1.722 ± 0.283
3.208PheSer: 3.208 ± 0.337
2.33PheThr: 2.33 ± 0.291
2.702PheVal: 2.702 ± 0.297
0.473PheTrp: 0.473 ± 0.126
1.756PheTyr: 1.756 ± 0.249
0.0PheXaa: 0.0 ± 0.0
Gly
4.356GlyAla: 4.356 ± 0.462
1.081GlyCys: 1.081 ± 0.244
3.951GlyAsp: 3.951 ± 0.422
4.39GlyGlu: 4.39 ± 0.356
3.276GlyPhe: 3.276 ± 0.313
3.174GlyGly: 3.174 ± 0.36
1.486GlyHis: 1.486 ± 0.269
4.762GlyIle: 4.762 ± 0.383
5.167GlyLys: 5.167 ± 0.459
4.39GlyLeu: 4.39 ± 0.451
1.857GlyMet: 1.857 ± 0.271
3.681GlyAsn: 3.681 ± 0.681
1.148GlyPro: 1.148 ± 0.187
2.06GlyGln: 2.06 ± 0.261
2.904GlyArg: 2.904 ± 0.257
4.39GlySer: 4.39 ± 0.414
3.478GlyThr: 3.478 ± 0.433
5.032GlyVal: 5.032 ± 0.402
0.912GlyTrp: 0.912 ± 0.175
3.208GlyTyr: 3.208 ± 0.35
0.0GlyXaa: 0.0 ± 0.0
His
1.182HisAla: 1.182 ± 0.197
0.371HisCys: 0.371 ± 0.107
1.283HisAsp: 1.283 ± 0.264
1.013HisGlu: 1.013 ± 0.211
0.642HisPhe: 0.642 ± 0.167
1.52HisGly: 1.52 ± 0.208
0.574HisHis: 0.574 ± 0.145
1.418HisIle: 1.418 ± 0.23
1.351HisLys: 1.351 ± 0.231
2.195HisLeu: 2.195 ± 0.312
0.405HisMet: 0.405 ± 0.126
1.114HisAsn: 1.114 ± 0.209
1.013HisPro: 1.013 ± 0.204
0.642HisGln: 0.642 ± 0.173
1.013HisArg: 1.013 ± 0.147
1.047HisSer: 1.047 ± 0.185
0.743HisThr: 0.743 ± 0.157
0.912HisVal: 0.912 ± 0.158
0.068HisTrp: 0.068 ± 0.042
0.642HisTyr: 0.642 ± 0.143
0.0HisXaa: 0.0 ± 0.0
Ile
4.458IleAla: 4.458 ± 0.38
0.81IleCys: 0.81 ± 0.197
4.762IleAsp: 4.762 ± 0.435
4.762IleGlu: 4.762 ± 0.351
2.263IlePhe: 2.263 ± 0.265
4.019IleGly: 4.019 ± 0.356
1.148IleHis: 1.148 ± 0.169
4.12IleIle: 4.12 ± 0.332
5.066IleLys: 5.066 ± 0.459
5.167IleLeu: 5.167 ± 0.453
1.52IleMet: 1.52 ± 0.259
4.052IleAsn: 4.052 ± 0.385
2.567IlePro: 2.567 ± 0.318
2.195IleGln: 2.195 ± 0.264
3.006IleArg: 3.006 ± 0.312
4.694IleSer: 4.694 ± 0.413
4.525IleThr: 4.525 ± 0.38
3.546IleVal: 3.546 ± 0.353
0.675IleTrp: 0.675 ± 0.146
2.263IleTyr: 2.263 ± 0.277
0.0IleXaa: 0.0 ± 0.0
Lys
6.349LysAla: 6.349 ± 0.54
0.507LysCys: 0.507 ± 0.117
4.93LysAsp: 4.93 ± 0.431
5.167LysGlu: 5.167 ± 0.461
3.613LysPhe: 3.613 ± 0.447
3.478LysGly: 3.478 ± 0.347
1.317LysHis: 1.317 ± 0.244
3.748LysIle: 3.748 ± 0.337
4.998LysLys: 4.998 ± 0.508
6.822LysLeu: 6.822 ± 0.603
2.229LysMet: 2.229 ± 0.314
3.884LysAsn: 3.884 ± 0.412
2.398LysPro: 2.398 ± 0.366
3.58LysGln: 3.58 ± 0.366
3.343LysArg: 3.343 ± 0.339
4.323LysSer: 4.323 ± 0.38
4.559LysThr: 4.559 ± 0.46
4.998LysVal: 4.998 ± 0.446
0.743LysTrp: 0.743 ± 0.176
3.141LysTyr: 3.141 ± 0.351
0.0LysXaa: 0.0 ± 0.0
Leu
6.72LeuAla: 6.72 ± 0.498
0.777LeuCys: 0.777 ± 0.131
6.18LeuAsp: 6.18 ± 0.458
7.328LeuGlu: 7.328 ± 0.522
2.702LeuPhe: 2.702 ± 0.345
5.133LeuGly: 5.133 ± 0.574
1.756LeuHis: 1.756 ± 0.233
5.032LeuIle: 5.032 ± 0.495
5.64LeuLys: 5.64 ± 0.41
5.944LeuLeu: 5.944 ± 0.519
2.026LeuMet: 2.026 ± 0.319
5.133LeuAsn: 5.133 ± 0.385
3.276LeuPro: 3.276 ± 0.367
3.107LeuGln: 3.107 ± 0.417
4.12LeuArg: 4.12 ± 0.349
5.302LeuSer: 5.302 ± 0.44
5.066LeuThr: 5.066 ± 0.472
4.863LeuVal: 4.863 ± 0.431
0.743LeuTrp: 0.743 ± 0.161
2.6LeuTyr: 2.6 ± 0.284
0.0LeuXaa: 0.0 ± 0.0
Met
1.385MetAla: 1.385 ± 0.224
0.236MetCys: 0.236 ± 0.075
1.351MetAsp: 1.351 ± 0.22
1.925MetGlu: 1.925 ± 0.251
0.946MetPhe: 0.946 ± 0.187
1.52MetGly: 1.52 ± 0.216
0.54MetHis: 0.54 ± 0.127
1.992MetIle: 1.992 ± 0.305
2.567MetLys: 2.567 ± 0.366
1.891MetLeu: 1.891 ± 0.296
0.54MetMet: 0.54 ± 0.151
0.946MetAsn: 0.946 ± 0.163
0.844MetPro: 0.844 ± 0.161
1.182MetGln: 1.182 ± 0.233
1.047MetArg: 1.047 ± 0.152
2.398MetSer: 2.398 ± 0.299
1.553MetThr: 1.553 ± 0.203
1.249MetVal: 1.249 ± 0.212
0.135MetTrp: 0.135 ± 0.063
0.844MetTyr: 0.844 ± 0.129
0.0MetXaa: 0.0 ± 0.0
Asn
3.546AsnAla: 3.546 ± 0.684
0.675AsnCys: 0.675 ± 0.169
2.465AsnAsp: 2.465 ± 0.285
2.735AsnGlu: 2.735 ± 0.318
2.128AsnPhe: 2.128 ± 0.288
4.39AsnGly: 4.39 ± 0.497
0.81AsnHis: 0.81 ± 0.186
3.782AsnIle: 3.782 ± 0.3
4.086AsnLys: 4.086 ± 0.422
4.897AsnLeu: 4.897 ± 0.377
1.148AsnMet: 1.148 ± 0.192
3.512AsnAsn: 3.512 ± 0.404
2.769AsnPro: 2.769 ± 0.335
1.655AsnGln: 1.655 ± 0.218
2.769AsnArg: 2.769 ± 0.334
3.985AsnSer: 3.985 ± 0.397
3.174AsnThr: 3.174 ± 0.285
3.546AsnVal: 3.546 ± 0.299
0.709AsnTrp: 0.709 ± 0.154
1.824AsnTyr: 1.824 ± 0.297
0.0AsnXaa: 0.0 ± 0.0
Pro
2.499ProAla: 2.499 ± 0.309
0.338ProCys: 0.338 ± 0.101
2.431ProAsp: 2.431 ± 0.256
3.276ProGlu: 3.276 ± 0.373
1.52ProPhe: 1.52 ± 0.264
2.229ProGly: 2.229 ± 0.325
0.574ProHis: 0.574 ± 0.151
2.128ProIle: 2.128 ± 0.29
2.195ProLys: 2.195 ± 0.275
2.161ProLeu: 2.161 ± 0.26
0.439ProMet: 0.439 ± 0.105
2.026ProAsn: 2.026 ± 0.301
1.317ProPro: 1.317 ± 0.247
1.081ProGln: 1.081 ± 0.196
1.486ProArg: 1.486 ± 0.227
2.06ProSer: 2.06 ± 0.277
2.06ProThr: 2.06 ± 0.257
2.195ProVal: 2.195 ± 0.265
0.473ProTrp: 0.473 ± 0.114
1.722ProTyr: 1.722 ± 0.234
0.0ProXaa: 0.0 ± 0.0
Gln
2.972GlnAla: 2.972 ± 0.504
0.507GlnCys: 0.507 ± 0.132
1.992GlnAsp: 1.992 ± 0.282
2.972GlnGlu: 2.972 ± 0.354
1.655GlnPhe: 1.655 ± 0.243
1.756GlnGly: 1.756 ± 0.17
0.507GlnHis: 0.507 ± 0.148
2.296GlnIle: 2.296 ± 0.291
2.499GlnLys: 2.499 ± 0.371
4.154GlnLeu: 4.154 ± 0.433
0.912GlnMet: 0.912 ± 0.16
1.722GlnAsn: 1.722 ± 0.217
0.675GlnPro: 0.675 ± 0.167
2.229GlnGln: 2.229 ± 0.37
1.891GlnArg: 1.891 ± 0.249
2.263GlnSer: 2.263 ± 0.367
2.06GlnThr: 2.06 ± 0.292
2.6GlnVal: 2.6 ± 0.289
0.608GlnTrp: 0.608 ± 0.175
1.655GlnTyr: 1.655 ± 0.191
0.0GlnXaa: 0.0 ± 0.0
Arg
3.039ArgAla: 3.039 ± 0.306
0.439ArgCys: 0.439 ± 0.121
2.87ArgAsp: 2.87 ± 0.247
3.208ArgGlu: 3.208 ± 0.344
1.689ArgPhe: 1.689 ± 0.264
3.681ArgGly: 3.681 ± 0.313
0.675ArgHis: 0.675 ± 0.176
2.938ArgIle: 2.938 ± 0.346
3.242ArgLys: 3.242 ± 0.425
3.884ArgLeu: 3.884 ± 0.364
1.283ArgMet: 1.283 ± 0.244
2.567ArgAsn: 2.567 ± 0.243
1.351ArgPro: 1.351 ± 0.202
1.824ArgGln: 1.824 ± 0.262
2.499ArgArg: 2.499 ± 0.298
2.634ArgSer: 2.634 ± 0.312
2.634ArgThr: 2.634 ± 0.266
3.107ArgVal: 3.107 ± 0.355
0.507ArgTrp: 0.507 ± 0.146
1.655ArgTyr: 1.655 ± 0.257
0.0ArgXaa: 0.0 ± 0.0
Ser
5.234SerAla: 5.234 ± 1.055
0.946SerCys: 0.946 ± 0.174
3.343SerAsp: 3.343 ± 0.336
4.052SerGlu: 4.052 ± 0.732
2.769SerPhe: 2.769 ± 0.246
5.403SerGly: 5.403 ± 0.566
0.878SerHis: 0.878 ± 0.175
4.221SerIle: 4.221 ± 0.438
4.829SerLys: 4.829 ± 0.381
6.045SerLeu: 6.045 ± 0.432
1.621SerMet: 1.621 ± 0.226
4.086SerAsn: 4.086 ± 0.457
1.925SerPro: 1.925 ± 0.254
2.195SerGln: 2.195 ± 0.304
2.938SerArg: 2.938 ± 0.333
4.221SerSer: 4.221 ± 0.454
3.546SerThr: 3.546 ± 0.346
3.917SerVal: 3.917 ± 0.35
1.047SerTrp: 1.047 ± 0.213
2.668SerTyr: 2.668 ± 0.3
0.0SerXaa: 0.0 ± 0.0
Thr
4.66ThrAla: 4.66 ± 0.708
0.439ThrCys: 0.439 ± 0.102
3.039ThrAsp: 3.039 ± 0.304
3.343ThrGlu: 3.343 ± 0.336
2.263ThrPhe: 2.263 ± 0.288
5.032ThrGly: 5.032 ± 0.504
1.081ThrHis: 1.081 ± 0.218
4.019ThrIle: 4.019 ± 0.424
4.221ThrLys: 4.221 ± 0.382
4.627ThrLeu: 4.627 ± 0.428
1.114ThrMet: 1.114 ± 0.183
3.512ThrAsn: 3.512 ± 0.494
2.533ThrPro: 2.533 ± 0.248
2.229ThrGln: 2.229 ± 0.267
2.499ThrArg: 2.499 ± 0.305
4.019ThrSer: 4.019 ± 0.609
2.972ThrThr: 2.972 ± 0.361
4.154ThrVal: 4.154 ± 0.376
0.574ThrTrp: 0.574 ± 0.141
2.128ThrTyr: 2.128 ± 0.262
0.0ThrXaa: 0.0 ± 0.0
Val
4.93ValAla: 4.93 ± 0.43
0.675ValCys: 0.675 ± 0.183
4.356ValAsp: 4.356 ± 0.364
3.917ValGlu: 3.917 ± 0.358
2.229ValPhe: 2.229 ± 0.246
3.85ValGly: 3.85 ± 0.397
1.148ValHis: 1.148 ± 0.234
4.221ValIle: 4.221 ± 0.418
4.66ValLys: 4.66 ± 0.379
4.525ValLeu: 4.525 ± 0.374
1.621ValMet: 1.621 ± 0.209
3.242ValAsn: 3.242 ± 0.443
2.634ValPro: 2.634 ± 0.322
2.465ValGln: 2.465 ± 0.319
2.431ValArg: 2.431 ± 0.289
4.525ValSer: 4.525 ± 0.423
4.221ValThr: 4.221 ± 0.443
4.052ValVal: 4.052 ± 0.442
0.709ValTrp: 0.709 ± 0.155
2.364ValTyr: 2.364 ± 0.302
0.0ValXaa: 0.0 ± 0.0
Trp
0.54TrpAla: 0.54 ± 0.137
0.304TrpCys: 0.304 ± 0.111
0.979TrpAsp: 0.979 ± 0.199
1.182TrpGlu: 1.182 ± 0.223
0.507TrpPhe: 0.507 ± 0.137
0.743TrpGly: 0.743 ± 0.186
0.203TrpHis: 0.203 ± 0.076
0.675TrpIle: 0.675 ± 0.164
1.013TrpLys: 1.013 ± 0.17
1.182TrpLeu: 1.182 ± 0.216
0.338TrpMet: 0.338 ± 0.113
0.54TrpAsn: 0.54 ± 0.142
0.135TrpPro: 0.135 ± 0.066
0.54TrpGln: 0.54 ± 0.151
0.54TrpArg: 0.54 ± 0.124
0.608TrpSer: 0.608 ± 0.169
0.574TrpThr: 0.574 ± 0.133
0.608TrpVal: 0.608 ± 0.13
0.203TrpTrp: 0.203 ± 0.103
0.439TrpTyr: 0.439 ± 0.139
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.195TyrAla: 2.195 ± 0.288
0.709TyrCys: 0.709 ± 0.154
2.972TyrAsp: 2.972 ± 0.312
2.06TyrGlu: 2.06 ± 0.275
1.722TyrPhe: 1.722 ± 0.276
2.567TyrGly: 2.567 ± 0.319
1.047TyrHis: 1.047 ± 0.183
2.769TyrIle: 2.769 ± 0.339
2.87TyrLys: 2.87 ± 0.317
3.006TyrLeu: 3.006 ± 0.356
1.013TyrMet: 1.013 ± 0.21
2.431TyrAsn: 2.431 ± 0.245
1.317TyrPro: 1.317 ± 0.183
1.553TyrGln: 1.553 ± 0.242
1.891TyrArg: 1.891 ± 0.273
2.6TyrSer: 2.6 ± 0.248
2.702TyrThr: 2.702 ± 0.307
2.094TyrVal: 2.094 ± 0.315
0.574TyrTrp: 0.574 ± 0.15
1.553TyrTyr: 1.553 ± 0.266
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 145 proteins (29613 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski