Maribellus luteus
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4999 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A399T8F4|A0A399T8F4_9BACT Uncharacterized protein OS=Maribellus luteus OX=2305463 GN=D1614_00420 PE=4 SV=1
MM1 pKa = 7.64 KK2 pKa = 10.29 KK3 pKa = 10.19 QIFNLFYY10 pKa = 11.15 ALALLAIVTACSDD23 pKa = 3.82 DD24 pKa = 4.86 DD25 pKa = 5.17 DD26 pKa = 6.47 PIDD29 pKa = 4.12 LPVYY33 pKa = 10.67 GDD35 pKa = 3.33 ATMTSFGFYY44 pKa = 10.65 VEE46 pKa = 5.24 DD47 pKa = 3.72 NEE49 pKa = 5.0 GVILKK54 pKa = 10.29 DD55 pKa = 3.67 YY56 pKa = 9.68 VAEE59 pKa = 5.29 GITGDD64 pKa = 3.81 FTVSVPDD71 pKa = 3.88 YY72 pKa = 10.49 VNKK75 pKa = 8.83 TALVARR81 pKa = 11.84 FVVSEE86 pKa = 3.95 NDD88 pKa = 3.29 TVYY91 pKa = 11.25 VNNVQQEE98 pKa = 4.53 SGVTANDD105 pKa = 3.85 FSAPLDD111 pKa = 3.96 YY112 pKa = 10.6 IVSEE116 pKa = 4.28 GTNNTKK122 pKa = 9.19 YY123 pKa = 9.27 TVTVANLPAAVWSLAASDD141 pKa = 3.8 TTDD144 pKa = 3.03 LRR146 pKa = 11.84 EE147 pKa = 3.62 FSMRR151 pKa = 11.84 VNPVTNVPYY160 pKa = 10.38 LAYY163 pKa = 10.28 ALDD166 pKa = 4.15 MDD168 pKa = 5.15 DD169 pKa = 5.1 SADD172 pKa = 3.57 EE173 pKa = 4.06 KK174 pKa = 11.53 VGVFKK179 pKa = 11.19 LDD181 pKa = 3.09 GNTLVNVGAKK191 pKa = 8.58 TVSEE195 pKa = 4.42 GRR197 pKa = 11.84 ASYY200 pKa = 10.08 PRR202 pKa = 11.84 IAFDD206 pKa = 4.2 AEE208 pKa = 3.95 GAPFISYY215 pKa = 11.16 ADD217 pKa = 3.63 YY218 pKa = 11.18 TNNDD222 pKa = 3.68 PYY224 pKa = 11.41 KK225 pKa = 10.39 EE226 pKa = 4.15 GSTTYY231 pKa = 10.42 AASVMNYY238 pKa = 9.92 NGSSWSYY245 pKa = 10.66 VGNKK249 pKa = 9.83 GITDD253 pKa = 3.32 VRR255 pKa = 11.84 ITYY258 pKa = 10.78 NDD260 pKa = 3.08 IVLKK264 pKa = 10.93 NDD266 pKa = 3.53 GNPMLFCYY274 pKa = 10.61 NNAAGTLARR283 pKa = 11.84 RR284 pKa = 11.84 EE285 pKa = 4.12 LNISDD290 pKa = 3.46 FTGNSWASNLTIPGRR305 pKa = 11.84 ASTQYY310 pKa = 11.18 AYY312 pKa = 8.73 HH313 pKa = 5.63 TTAKK317 pKa = 10.42 NVDD320 pKa = 3.1 GVIYY324 pKa = 10.51 LGVYY328 pKa = 8.63 NANEE332 pKa = 4.38 GTFSVYY338 pKa = 10.94 KK339 pKa = 9.71 NTDD342 pKa = 3.36 GSWTTIVEE350 pKa = 4.47 SYY352 pKa = 10.42 LQDD355 pKa = 3.34 GATTGNLRR363 pKa = 11.84 DD364 pKa = 3.51 FDD366 pKa = 4.1 MDD368 pKa = 3.0 VDD370 pKa = 3.79 RR371 pKa = 11.84 DD372 pKa = 3.99 GNVFVCVADD381 pKa = 4.45 DD382 pKa = 3.47 EE383 pKa = 5.12 AGADD387 pKa = 3.15 IYY389 pKa = 10.89 RR390 pKa = 11.84 PKK392 pKa = 9.91 VLKK395 pKa = 10.7 YY396 pKa = 10.57 DD397 pKa = 3.54 ATAEE401 pKa = 4.18 TWSKK405 pKa = 11.0 VGTPIAIDD413 pKa = 4.07 FSTTRR418 pKa = 11.84 EE419 pKa = 3.64 FSLAISPVGTPFLMYY434 pKa = 9.9 RR435 pKa = 11.84 DD436 pKa = 4.31 DD437 pKa = 4.49 SMFPVVVSFDD447 pKa = 4.36 EE448 pKa = 4.57 DD449 pKa = 3.89 TQDD452 pKa = 2.75 WTTPAQLNAVEE463 pKa = 4.81 AEE465 pKa = 4.02 NLYY468 pKa = 10.07 MDD470 pKa = 4.65 FAPNGVAYY478 pKa = 10.03 AAFTTSATGEE488 pKa = 4.29 TMLFKK493 pKa = 10.91 YY494 pKa = 9.83 DD495 pKa = 3.46 IPAEE499 pKa = 4.05
Molecular weight: 54.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.935
IPC_protein 3.973
Toseland 3.745
ProMoST 4.113
Dawson 3.961
Bjellqvist 4.126
Wikipedia 3.897
Rodwell 3.795
Grimsley 3.643
Solomon 3.961
Lehninger 3.923
Nozaki 4.062
DTASelect 4.342
Thurlkill 3.795
EMBOSS 3.91
Sillero 4.088
Patrickios 1.202
IPC_peptide 3.961
IPC2_peptide 4.062
IPC2.peptide.svr19 3.963
Protein with the highest isoelectric point:
>tr|A0A399SQ95|A0A399SQ95_9BACT CoA transferase (Fragment) OS=Maribellus luteus OX=2305463 GN=D1614_24595 PE=4 SV=1
AA1 pKa = 6.75 QGKK4 pKa = 9.28 RR5 pKa = 11.84 RR6 pKa = 11.84 LHH8 pKa = 6.2 RR9 pKa = 11.84 GGTGLSARR17 pKa = 11.84 RR18 pKa = 11.84 GARR21 pKa = 11.84 PRR23 pKa = 11.84 LGGTAPPPRR32 pKa = 11.84 PPAKK36 pKa = 10.46 ASGKK40 pKa = 8.58 PGRR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 PQLSAGLAVATGSRR59 pKa = 11.84 RR60 pKa = 11.84 RR61 pKa = 11.84 VRR63 pKa = 11.84 RR64 pKa = 11.84 AAPIGGPLRR73 pKa = 11.84 RR74 pKa = 11.84 DD75 pKa = 3.17 RR76 pKa = 11.84 PAPARR81 pKa = 11.84 RR82 pKa = 11.84 GRR84 pKa = 11.84 RR85 pKa = 11.84 RR86 pKa = 11.84 GRR88 pKa = 11.84 DD89 pKa = 2.97 RR90 pKa = 11.84 NGRR93 pKa = 11.84 RR94 pKa = 11.84 SRR96 pKa = 11.84 SGRR99 pKa = 11.84 RR100 pKa = 11.84 GPAHH104 pKa = 7.46 PGRR107 pKa = 11.84 HH108 pKa = 5.39 PAAGRR113 pKa = 11.84 GAARR117 pKa = 11.84 RR118 pKa = 11.84 RR119 pKa = 11.84 RR120 pKa = 11.84 SAGARR125 pKa = 11.84 RR126 pKa = 11.84 APTARR131 pKa = 11.84 RR132 pKa = 11.84 RR133 pKa = 11.84 RR134 pKa = 11.84 RR135 pKa = 11.84 RR136 pKa = 11.84 PLRR139 pKa = 11.84 NRR141 pKa = 11.84 TPGRR145 pKa = 11.84 RR146 pKa = 11.84 ARR148 pKa = 11.84 SPRR151 pKa = 11.84 RR152 pKa = 11.84 SARR155 pKa = 11.84 PGAGLRR161 pKa = 11.84 RR162 pKa = 11.84 LGPDD166 pKa = 2.43 RR167 pKa = 11.84 LFPEE171 pKa = 5.05 RR172 pKa = 11.84 PPP174 pKa = 3.72
Molecular weight: 19.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.852
Toseland 13.013
ProMoST 13.51
Dawson 13.013
Bjellqvist 13.013
Wikipedia 13.495
Rodwell 12.544
Grimsley 13.056
Solomon 13.525
Lehninger 13.422
Nozaki 13.013
DTASelect 13.013
Thurlkill 13.013
EMBOSS 13.525
Sillero 13.013
Patrickios 12.252
IPC_peptide 13.525
IPC2_peptide 12.515
IPC2.peptide.svr19 9.221
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4999
0
4999
1801786
18
3326
360.4
40.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.962 ± 0.035
0.86 ± 0.012
5.528 ± 0.03
6.782 ± 0.032
5.052 ± 0.025
6.957 ± 0.031
1.829 ± 0.015
6.864 ± 0.029
6.693 ± 0.036
9.215 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.39 ± 0.014
5.431 ± 0.029
3.874 ± 0.019
3.508 ± 0.02
4.151 ± 0.023
6.36 ± 0.025
5.412 ± 0.032
6.641 ± 0.027
1.384 ± 0.014
4.108 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here