Tropicimonas sp. IMCC6043
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5038 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q3Z7Z2|A0A4Q3Z7Z2_9RHOB Nucleoside triphosphate pyrophosphohydrolase OS=Tropicimonas sp. IMCC6043 OX=2510645 GN=EU800_12730 PE=4 SV=1
MM1 pKa = 6.42 FTSRR5 pKa = 11.84 IAICTAISMLALAGTASAQSAGEE28 pKa = 3.87 WTLGFGAAYY37 pKa = 10.78 SMPKK41 pKa = 9.48 YY42 pKa = 10.51 DD43 pKa = 5.0 NGYY46 pKa = 9.84 FDD48 pKa = 5.97 PDD50 pKa = 3.53 NTSSNQTVTVSDD62 pKa = 3.77 TASLTFTGEE71 pKa = 3.63 YY72 pKa = 10.01 FIWDD76 pKa = 3.59 NVGVEE81 pKa = 4.53 LLVPLPFSHH90 pKa = 6.84 SADD93 pKa = 3.38 VEE95 pKa = 4.64 DD96 pKa = 3.99 YY97 pKa = 11.18 GKK99 pKa = 10.61 AVDD102 pKa = 4.13 LTYY105 pKa = 10.2 FAPTLSLQYY114 pKa = 10.23 HH115 pKa = 5.79 FRR117 pKa = 11.84 ASEE120 pKa = 3.99 QISYY124 pKa = 10.87 LIGLGVNYY132 pKa = 9.51 STFTDD137 pKa = 3.64 VSTSGDD143 pKa = 3.79 LKK145 pKa = 11.38 GLDD148 pKa = 3.87 LDD150 pKa = 4.03 VDD152 pKa = 3.9 NSFGGALHH160 pKa = 7.12 LGLDD164 pKa = 3.83 YY165 pKa = 11.16 WMSDD169 pKa = 3.28 DD170 pKa = 3.79 SAIRR174 pKa = 11.84 ADD176 pKa = 3.91 MRR178 pKa = 11.84 WISIDD183 pKa = 3.12 TDD185 pKa = 3.35 ADD187 pKa = 3.58 AGGSRR192 pKa = 11.84 VGEE195 pKa = 3.96 IEE197 pKa = 3.93 VDD199 pKa = 3.63 PFILGLSYY207 pKa = 10.32 IRR209 pKa = 11.84 KK210 pKa = 8.69 FF211 pKa = 3.35
Molecular weight: 22.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.923
IPC_protein 3.935
Toseland 3.706
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.101
Wikipedia 3.897
Rodwell 3.757
Grimsley 3.617
Solomon 3.935
Lehninger 3.897
Nozaki 4.05
DTASelect 4.342
Thurlkill 3.77
EMBOSS 3.91
Sillero 4.062
Patrickios 1.138
IPC_peptide 3.923
IPC2_peptide 4.037
IPC2.peptide.svr19 3.926
Protein with the highest isoelectric point:
>tr|A0A4V1V6Q4|A0A4V1V6Q4_9RHOB Peptidoglycan-binding protein OS=Tropicimonas sp. IMCC6043 OX=2510645 GN=EU800_12775 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5038
0
5038
1561054
24
10442
309.9
33.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.638 ± 0.049
0.905 ± 0.013
5.865 ± 0.035
6.194 ± 0.03
3.762 ± 0.023
8.83 ± 0.043
2.001 ± 0.018
5.116 ± 0.025
2.702 ± 0.03
10.229 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.648 ± 0.022
2.401 ± 0.022
5.247 ± 0.032
2.779 ± 0.015
7.174 ± 0.046
5.245 ± 0.032
5.345 ± 0.032
7.369 ± 0.03
1.41 ± 0.016
2.142 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here