Streptococcus satellite phage Javan463

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZP56|A0A4D5ZP56_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan463 OX=2558714 GN=JavanS463_0013 PE=4 SV=1
MM1 pKa = 7.72AKK3 pKa = 10.2NSPQDD8 pKa = 3.89LINRR12 pKa = 11.84KK13 pKa = 8.98IFSTNLNMLMAKK25 pKa = 10.2KK26 pKa = 10.1NIKK29 pKa = 10.21QIDD32 pKa = 3.46IHH34 pKa = 6.66NKK36 pKa = 9.74LGIPKK41 pKa = 8.5STITGYY47 pKa = 10.91VKK49 pKa = 10.57GRR51 pKa = 11.84SLPTAGNVQKK61 pKa = 10.8LADD64 pKa = 3.93FFGVLKK70 pKa = 10.84SDD72 pKa = 3.19IDD74 pKa = 3.86PRR76 pKa = 11.84FDD78 pKa = 4.03SNNIEE83 pKa = 4.29TNSNIIPSTLQKK95 pKa = 9.47VTSTLSQLEE104 pKa = 4.51HH105 pKa = 6.54KK106 pKa = 10.36RR107 pKa = 11.84QLNVLDD113 pKa = 3.87YY114 pKa = 11.66AEE116 pKa = 4.47TQLEE120 pKa = 4.14QQNTVEE126 pKa = 4.75EE127 pKa = 4.39PQATYY132 pKa = 8.87YY133 pKa = 9.48TYY135 pKa = 11.16NYY137 pKa = 9.02YY138 pKa = 11.1DD139 pKa = 3.61HH140 pKa = 7.34AASAGTGQYY149 pKa = 11.03LNDD152 pKa = 3.69VQVEE156 pKa = 4.58TIEE159 pKa = 5.25LPVDD163 pKa = 3.45YY164 pKa = 10.6DD165 pKa = 3.94ADD167 pKa = 3.98FVIPVYY173 pKa = 10.34GDD175 pKa = 3.3SMEE178 pKa = 4.56PEE180 pKa = 4.48YY181 pKa = 11.08HH182 pKa = 6.24SGDD185 pKa = 3.53YY186 pKa = 11.17VFIKK190 pKa = 10.62LSINLSDD197 pKa = 3.52GDD199 pKa = 3.54IGVFEE204 pKa = 4.94YY205 pKa = 11.34YY206 pKa = 10.64GDD208 pKa = 4.18AYY210 pKa = 9.96IKK212 pKa = 10.44QLVINDD218 pKa = 3.53SGAFLHH224 pKa = 6.49SLNDD228 pKa = 3.42KK229 pKa = 10.89YY230 pKa = 11.69DD231 pKa = 5.24DD232 pKa = 3.68ILIDD236 pKa = 3.7RR237 pKa = 11.84DD238 pKa = 3.23SDD240 pKa = 3.49FRR242 pKa = 11.84IIGEE246 pKa = 4.32VIGSFTSKK254 pKa = 10.46SS255 pKa = 3.33

Molecular weight: 28.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZTZ1|A0A4D5ZTZ1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan463 OX=2558714 GN=JavanS463_0011 PE=4 SV=1
MM1 pKa = 7.31IMKK4 pKa = 8.92ITEE7 pKa = 4.11HH8 pKa = 6.3KK9 pKa = 9.71KK10 pKa = 9.75KK11 pKa = 10.59NGTIVYY17 pKa = 9.02RR18 pKa = 11.84ASIYY22 pKa = 10.8LGIDD26 pKa = 2.83QMTGKK31 pKa = 10.05RR32 pKa = 11.84VKK34 pKa = 9.97TSITGRR40 pKa = 11.84TRR42 pKa = 11.84KK43 pKa = 9.11EE44 pKa = 3.7VNQKK48 pKa = 10.29AKK50 pKa = 10.21HH51 pKa = 5.3AQFDD55 pKa = 4.08FLSNGSTIKK64 pKa = 10.43RR65 pKa = 11.84KK66 pKa = 9.88VVIKK70 pKa = 8.87TFKK73 pKa = 10.26EE74 pKa = 4.46LSHH77 pKa = 6.68LWLEE81 pKa = 4.6TYY83 pKa = 10.71KK84 pKa = 10.43LTVKK88 pKa = 10.0PQTYY92 pKa = 9.55DD93 pKa = 3.09ATVTRR98 pKa = 11.84LNRR101 pKa = 11.84HH102 pKa = 5.38IMPTLGNMKK111 pKa = 9.51VDD113 pKa = 4.87KK114 pKa = 9.89ITASDD119 pKa = 3.12IQMLINRR126 pKa = 11.84LSKK129 pKa = 11.11YY130 pKa = 8.49YY131 pKa = 11.04VNYY134 pKa = 8.12TAVRR138 pKa = 11.84SVIRR142 pKa = 11.84KK143 pKa = 8.39VLQQGVLLGLIDD155 pKa = 3.95YY156 pKa = 10.62NSARR160 pKa = 11.84DD161 pKa = 3.44IILPRR166 pKa = 11.84KK167 pKa = 8.91QPNAKK172 pKa = 9.88KK173 pKa = 9.75KK174 pKa = 10.82VKK176 pKa = 10.53FIDD179 pKa = 4.16PSDD182 pKa = 3.48LKK184 pKa = 11.33SFLEE188 pKa = 4.04HH189 pKa = 7.4LEE191 pKa = 4.21TSQHH195 pKa = 5.32KK196 pKa = 10.23RR197 pKa = 11.84YY198 pKa = 10.13NLYY201 pKa = 10.34FDD203 pKa = 4.37AVLYY207 pKa = 10.4QLLLSTGLRR216 pKa = 11.84IGEE219 pKa = 4.13ACALEE224 pKa = 4.31WGDD227 pKa = 3.94IDD229 pKa = 5.91LEE231 pKa = 4.47NGTIAINKK239 pKa = 7.38TYY241 pKa = 11.06NKK243 pKa = 9.77NLKK246 pKa = 9.76FLSTAKK252 pKa = 8.49TQSGNRR258 pKa = 11.84VISVDD263 pKa = 3.32KK264 pKa = 10.13KK265 pKa = 7.7TLRR268 pKa = 11.84SLKK271 pKa = 9.82LYY273 pKa = 9.48QMRR276 pKa = 11.84QRR278 pKa = 11.84QLFNEE283 pKa = 3.92VGARR287 pKa = 11.84VSEE290 pKa = 4.58VVFATPTRR298 pKa = 11.84KK299 pKa = 9.82YY300 pKa = 9.68FNASVRR306 pKa = 11.84QSALDD311 pKa = 3.79TRR313 pKa = 11.84CKK315 pKa = 9.91EE316 pKa = 3.83AGIEE320 pKa = 3.94RR321 pKa = 11.84FTFHH325 pKa = 7.65AFRR328 pKa = 11.84HH329 pKa = 4.69THH331 pKa = 6.92ASLLLNAGISYY342 pKa = 10.68KK343 pKa = 10.23EE344 pKa = 3.61LQYY347 pKa = 11.51RR348 pKa = 11.84LGHH351 pKa = 6.41ANISMTLDD359 pKa = 3.43TYY361 pKa = 11.88GHH363 pKa = 6.98LSKK366 pKa = 11.01DD367 pKa = 3.47KK368 pKa = 10.63EE369 pKa = 4.32KK370 pKa = 11.01EE371 pKa = 3.74AVLYY375 pKa = 9.78YY376 pKa = 10.38EE377 pKa = 4.79KK378 pKa = 11.4AMNNLL383 pKa = 3.41

Molecular weight: 44.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23

0

23

4053

46

545

176.2

20.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.798 ± 0.265

0.691 ± 0.231

5.65 ± 0.408

7.525 ± 0.611

3.997 ± 0.215

4.787 ± 0.436

2.073 ± 0.226

7.427 ± 0.468

9.055 ± 0.513

9.771 ± 0.474

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.27 ± 0.246

5.255 ± 0.36

3.133 ± 0.405

4.762 ± 0.233

5.009 ± 0.469

5.576 ± 0.363

6.168 ± 0.347

5.157 ± 0.32

0.888 ± 0.164

5.009 ± 0.385
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski