Pontibacter sp. GY10130
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4726 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C8KB90|A0A5C8KB90_9BACT Uncharacterized protein OS=Pontibacter sp. GY10130 OX=2603253 GN=FVR03_03740 PE=4 SV=1
MM1 pKa = 7.18 TPHH4 pKa = 7.43 IILLTQEE11 pKa = 4.44 PNADD15 pKa = 2.81 MRR17 pKa = 11.84 FYY19 pKa = 9.3 YY20 pKa = 10.13 TADD23 pKa = 3.23 IAHH26 pKa = 6.85 TDD28 pKa = 3.36 DD29 pKa = 5.77 DD30 pKa = 4.46 GALKK34 pKa = 10.41 VFPSFDD40 pKa = 3.24 AAADD44 pKa = 3.69 EE45 pKa = 4.84 RR46 pKa = 11.84 DD47 pKa = 3.6 DD48 pKa = 5.65 LEE50 pKa = 5.6 IDD52 pKa = 4.06 GYY54 pKa = 10.65 IIEE57 pKa = 4.62 LPIYY61 pKa = 10.28 SII63 pKa = 4.52
Molecular weight: 7.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.735
IPC2_protein 3.808
IPC_protein 3.757
Toseland 3.541
ProMoST 3.961
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.465
Solomon 3.757
Lehninger 3.706
Nozaki 3.91
DTASelect 4.151
Thurlkill 3.63
EMBOSS 3.757
Sillero 3.884
Patrickios 1.901
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.811
Protein with the highest isoelectric point:
>tr|A0A5C8J567|A0A5C8J567_9BACT Uncharacterized protein OS=Pontibacter sp. GY10130 OX=2603253 GN=FVR03_20020 PE=4 SV=1
MM1 pKa = 7.52 LLFRR5 pKa = 11.84 RR6 pKa = 11.84 KK7 pKa = 9.42 SALDD11 pKa = 3.56 KK12 pKa = 10.95 PPEE15 pKa = 4.45 GSDD18 pKa = 3.73 ARR20 pKa = 11.84 DD21 pKa = 3.02 KK22 pKa = 11.04 AARR25 pKa = 11.84 AIAAVIVNRR34 pKa = 11.84 QLQLAKK40 pKa = 9.92 CCSSRR45 pKa = 11.84 YY46 pKa = 8.97 EE47 pKa = 3.98 RR48 pKa = 11.84 LSLGVRR54 pKa = 11.84 KK55 pKa = 9.65 ALLLLLCMLFGGTSLWFLARR75 pKa = 11.84 GVWPEE80 pKa = 3.71 QMQQALIHH88 pKa = 5.08 VQSYY92 pKa = 10.27 KK93 pKa = 10.28 PDD95 pKa = 3.36 NRR97 pKa = 11.84 PIAGPNLRR105 pKa = 11.84 DD106 pKa = 3.53 SLNRR110 pKa = 11.84 QHH112 pKa = 6.96 NLPLKK117 pKa = 10.3 KK118 pKa = 10.49
Molecular weight: 13.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.619
IPC_protein 10.189
Toseland 10.774
ProMoST 10.452
Dawson 10.847
Bjellqvist 10.526
Wikipedia 11.023
Rodwell 11.155
Grimsley 10.877
Solomon 10.965
Lehninger 10.935
Nozaki 10.774
DTASelect 10.511
Thurlkill 10.76
EMBOSS 11.169
Sillero 10.774
Patrickios 10.906
IPC_peptide 10.979
IPC2_peptide 9.575
IPC2.peptide.svr19 8.657
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4726
0
4726
1649456
14
3193
349.0
39.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.224 ± 0.035
0.738 ± 0.012
4.961 ± 0.027
6.312 ± 0.041
4.641 ± 0.025
6.921 ± 0.034
2.059 ± 0.019
5.983 ± 0.033
5.954 ± 0.037
10.079 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.272 ± 0.016
4.888 ± 0.038
4.155 ± 0.023
4.434 ± 0.026
4.571 ± 0.026
6.006 ± 0.028
5.86 ± 0.037
6.826 ± 0.026
1.204 ± 0.016
3.912 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here