Nosema bombycis (strain CQ1 / CVCC 102059) (Microsporidian parasite) (Pebrine of silkworm)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Apansporoblastina; Nosematidae; Nosema; Nosema bombycis

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4398 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R0KN11|R0KN11_NOSB1 Uncharacterized protein OS=Nosema bombycis (strain CQ1 / CVCC 102059) OX=578461 GN=NBO_1103g0002 PE=4 SV=1
MM1 pKa = 7.63EE2 pKa = 6.0IDD4 pKa = 3.76TNVLSTQYY12 pKa = 10.38TPLDD16 pKa = 3.66IKK18 pKa = 11.06FKK20 pKa = 10.19TCDD23 pKa = 3.09FDD25 pKa = 5.32FFSDD29 pKa = 3.91CTNNTLVEE37 pKa = 4.15LCNASNYY44 pKa = 10.38LNYY47 pKa = 10.11PYY49 pKa = 10.8LLEE52 pKa = 4.23LTCKK56 pKa = 10.17ILANRR61 pKa = 11.84MQYY64 pKa = 10.5RR65 pKa = 11.84PTEE68 pKa = 3.99EE69 pKa = 3.81LRR71 pKa = 11.84EE72 pKa = 4.27LIGQGNEE79 pKa = 4.07IIEE82 pKa = 4.62DD83 pKa = 3.77TDD85 pKa = 4.31DD86 pKa = 5.49DD87 pKa = 4.38INKK90 pKa = 9.06EE91 pKa = 4.21LEE93 pKa = 4.06WLSSSEE99 pKa = 3.97

Molecular weight: 11.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R0MFE7|R0MFE7_NOSB1 Uncharacterized protein OS=Nosema bombycis (strain CQ1 / CVCC 102059) OX=578461 GN=NBO_1151g0001 PE=4 SV=1
MM1 pKa = 7.58LFFNMLYY8 pKa = 10.25HH9 pKa = 6.55FCSSLKK15 pKa = 10.84SFITSTTSSKK25 pKa = 11.01SNRR28 pKa = 11.84IFTNIRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84NKK38 pKa = 9.7GIIVIICLSLLSCSLSPSS56 pKa = 3.21

Molecular weight: 6.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4398

0

4398

1099605

49

1871

250.0

28.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.062 ± 0.035

1.705 ± 0.018

5.91 ± 0.029

7.576 ± 0.046

5.838 ± 0.042

4.138 ± 0.04

1.762 ± 0.018

8.812 ± 0.039

10.131 ± 0.054

9.842 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.193 ± 0.016

7.204 ± 0.04

3.127 ± 0.035

2.684 ± 0.022

3.706 ± 0.023

7.416 ± 0.039

4.808 ± 0.03

5.308 ± 0.03

0.573 ± 0.009

4.204 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski