Amino acid dipepetide frequency for Nosema bombycis (strain CQ1 / CVCC 102059) (Microsporidian parasite) (Pebrine of silkworm)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	1.323AlaAla: 1.323 ± 0.043	0.574AlaCys: 0.574 ± 0.023	1.586AlaAsp: 1.586 ± 0.045	1.862AlaGlu: 1.862 ± 0.05	1.874AlaPhe: 1.874 ± 0.043	1.421AlaGly: 1.421 ± 0.042	0.566AlaHis: 0.566 ± 0.025	2.817AlaIle: 2.817 ± 0.058	2.587AlaLys: 2.587 ± 0.059	3.398AlaLeu: 3.398 ± 0.066	0.742AlaMet: 0.742 ± 0.027	1.678AlaAsn: 1.678 ± 0.045	0.979AlaPro: 0.979 ± 0.036	1.004AlaGln: 1.004 ± 0.043	1.203AlaArg: 1.203 ± 0.039	2.257AlaSer: 2.257 ± 0.052	1.451AlaThr: 1.451 ± 0.04	1.822AlaVal: 1.822 ± 0.042	0.188AlaTrp: 0.188 ± 0.014	1.287AlaTyr: 1.287 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	0.578CysAla: 0.578 ± 0.023	0.397CysCys: 0.397 ± 0.022	1.09CysAsp: 1.09 ± 0.036	1.066CysGlu: 1.066 ± 0.034	1.064CysPhe: 1.064 ± 0.032	0.95CysGly: 0.95 ± 0.032	0.241CysHis: 0.241 ± 0.013	1.461CysIle: 1.461 ± 0.034	1.536CysLys: 1.536 ± 0.037	1.879CysLeu: 1.879 ± 0.048	0.327CysMet: 0.327 ± 0.019	0.959CysAsn: 0.959 ± 0.027	0.56CysPro: 0.56 ± 0.024	0.346CysGln: 0.346 ± 0.017	0.638CysArg: 0.638 ± 0.025	1.343CysSer: 1.343 ± 0.037	0.821CysThr: 0.821 ± 0.025	1.024CysVal: 1.024 ± 0.036	0.091CysTrp: 0.091 ± 0.008	0.679CysTyr: 0.679 ± 0.024	0.0CysXaa: 0.0 ± 0.0
Asp	1.581AspAla: 1.581 ± 0.044	1.024AspCys: 1.024 ± 0.03	3.572AspAsp: 3.572 ± 0.064	4.535AspGlu: 4.535 ± 0.073	4.115AspPhe: 4.115 ± 0.069	2.28AspGly: 2.28 ± 0.054	1.09AspHis: 1.09 ± 0.034	4.762AspIle: 4.762 ± 0.07	6.026AspLys: 6.026 ± 0.091	7.153AspLeu: 7.153 ± 0.092	0.992AspMet: 0.992 ± 0.033	3.919AspAsn: 3.919 ± 0.058	1.71AspPro: 1.71 ± 0.041	1.646AspGln: 1.646 ± 0.045	1.741AspArg: 1.741 ± 0.038	4.153AspSer: 4.153 ± 0.069	2.528AspThr: 2.528 ± 0.058	3.189AspVal: 3.189 ± 0.053	0.401AspTrp: 0.401 ± 0.019	2.685AspTyr: 2.685 ± 0.047	0.001AspXaa: 0.001 ± 0.001
Glu	2.344GluAla: 2.344 ± 0.061	1.17GluCys: 1.17 ± 0.039	5.453GluAsp: 5.453 ± 0.074	7.319GluGlu: 7.319 ± 0.129	3.49GluPhe: 3.49 ± 0.054	3.313GluGly: 3.313 ± 0.075	1.267GluHis: 1.267 ± 0.032	7.885GluIle: 7.885 ± 0.094	7.93GluLys: 7.93 ± 0.1	5.574GluLeu: 5.574 ± 0.086	2.078GluMet: 2.078 ± 0.052	6.414GluAsn: 6.414 ± 0.081	1.626GluPro: 1.626 ± 0.044	1.794GluGln: 1.794 ± 0.041	2.63GluArg: 2.63 ± 0.06	4.952GluSer: 4.952 ± 0.077	3.184GluThr: 3.184 ± 0.063	4.104GluVal: 4.104 ± 0.06	0.385GluTrp: 0.385 ± 0.019	2.852GluTyr: 2.852 ± 0.051	0.0GluXaa: 0.0 ± 0.0
Phe	1.463PheAla: 1.463 ± 0.042	1.232PheCys: 1.232 ± 0.036	3.495PheAsp: 3.495 ± 0.056	4.043PheGlu: 4.043 ± 0.068	3.535PhePhe: 3.535 ± 0.077	2.364PheGly: 2.364 ± 0.055	0.913PheHis: 0.913 ± 0.034	5.7PheIle: 5.7 ± 0.081	5.546PheLys: 5.546 ± 0.075	6.274PheLeu: 6.274 ± 0.103	1.411PheMet: 1.411 ± 0.034	3.973PheAsn: 3.973 ± 0.069	1.424PhePro: 1.424 ± 0.038	1.125PheGln: 1.125 ± 0.035	1.838PheArg: 1.838 ± 0.042	4.153PheSer: 4.153 ± 0.072	2.963PheThr: 2.963 ± 0.051	3.18PheVal: 3.18 ± 0.057	0.356PheTrp: 0.356 ± 0.02	3.394PheTyr: 3.394 ± 0.071	0.001PheXaa: 0.001 ± 0.001
Gly	1.342GlyAla: 1.342 ± 0.044	0.78GlyCys: 0.78 ± 0.029	2.722GlyAsp: 2.722 ± 0.056	2.862GlyGlu: 2.862 ± 0.059	2.354GlyPhe: 2.354 ± 0.048	2.368GlyGly: 2.368 ± 0.074	0.859GlyHis: 0.859 ± 0.029	3.453GlyIle: 3.453 ± 0.064	3.559GlyLys: 3.559 ± 0.066	3.94GlyLeu: 3.94 ± 0.061	0.918GlyMet: 0.918 ± 0.03	2.587GlyAsn: 2.587 ± 0.051	1.04GlyPro: 1.04 ± 0.033	1.076GlyGln: 1.076 ± 0.048	1.747GlyArg: 1.747 ± 0.047	3.07GlySer: 3.07 ± 0.087	2.061GlyThr: 2.061 ± 0.052	2.64GlyVal: 2.64 ± 0.052	0.296GlyTrp: 0.296 ± 0.018	1.702GlyTyr: 1.702 ± 0.041	0.002GlyXaa: 0.002 ± 0.001
His	0.516HisAla: 0.516 ± 0.021	0.281HisCys: 0.281 ± 0.016	0.887HisAsp: 0.887 ± 0.03	1.094HisGlu: 1.094 ± 0.032	1.282HisPhe: 1.282 ± 0.039	0.774HisGly: 0.774 ± 0.03	0.518HisHis: 0.518 ± 0.03	1.353HisIle: 1.353 ± 0.032	1.754HisLys: 1.754 ± 0.044	1.916HisLeu: 1.916 ± 0.043	0.317HisMet: 0.317 ± 0.018	1.185HisAsn: 1.185 ± 0.041	0.749HisPro: 0.749 ± 0.024	0.453HisGln: 0.453 ± 0.022	0.641HisArg: 0.641 ± 0.026	1.379HisSer: 1.379 ± 0.034	0.845HisThr: 0.845 ± 0.028	0.937HisVal: 0.937 ± 0.029	0.104HisTrp: 0.104 ± 0.01	0.633HisTyr: 0.633 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	2.535IleAla: 2.535 ± 0.051	1.644IleCys: 1.644 ± 0.038	4.96IleAsp: 4.96 ± 0.066	6.892IleGlu: 6.892 ± 0.081	5.41IlePhe: 5.41 ± 0.084	3.249IleGly: 3.249 ± 0.064	1.548IleHis: 1.548 ± 0.041	6.848IleIle: 6.848 ± 0.087	9.451IleLys: 9.451 ± 0.112	9.214IleLeu: 9.214 ± 0.12	1.739IleMet: 1.739 ± 0.043	6.369IleAsn: 6.369 ± 0.09	2.896IlePro: 2.896 ± 0.047	2.506IleGln: 2.506 ± 0.047	3.254IleArg: 3.254 ± 0.058	6.764IleSer: 6.764 ± 0.084	4.11IleThr: 4.11 ± 0.066	4.315IleVal: 4.315 ± 0.076	0.495IleTrp: 0.495 ± 0.021	3.919IleTyr: 3.919 ± 0.068	0.0IleXaa: 0.0 ± 0.0
Lys	3.191LysAla: 3.191 ± 0.062	1.39LysCys: 1.39 ± 0.042	6.554LysAsp: 6.554 ± 0.096	9.083LysGlu: 9.083 ± 0.132	4.59LysPhe: 4.59 ± 0.066	4.188LysGly: 4.188 ± 0.08	1.619LysHis: 1.619 ± 0.039	10.107LysIle: 10.107 ± 0.091	11.163LysLys: 11.163 ± 0.121	7.678LysLeu: 7.678 ± 0.1	2.806LysMet: 2.806 ± 0.051	8.161LysAsn: 8.161 ± 0.085	2.719LysPro: 2.719 ± 0.066	2.545LysGln: 2.545 ± 0.047	4.08LysArg: 4.08 ± 0.061	6.942LysSer: 6.942 ± 0.092	5.074LysThr: 5.074 ± 0.073	4.807LysVal: 4.807 ± 0.075	0.577LysTrp: 0.577 ± 0.023	4.035LysTyr: 4.035 ± 0.065	0.002LysXaa: 0.002 ± 0.001
Leu	2.953LeuAla: 2.953 ± 0.064	1.802LeuCys: 1.802 ± 0.042	5.549LeuAsp: 5.549 ± 0.087	7.185LeuGlu: 7.185 ± 0.094	5.965LeuPhe: 5.965 ± 0.085	3.785LeuGly: 3.785 ± 0.074	1.572LeuHis: 1.572 ± 0.036	8.95LeuIle: 8.95 ± 0.101	11.066LeuLys: 11.066 ± 0.121	9.613LeuLeu: 9.613 ± 0.111	2.099LeuMet: 2.099 ± 0.044	7.583LeuAsn: 7.583 ± 0.097	2.902LeuPro: 2.902 ± 0.058	2.46LeuGln: 2.46 ± 0.052	3.876LeuArg: 3.876 ± 0.069	7.497LeuSer: 7.497 ± 0.094	4.27LeuThr: 4.27 ± 0.071	4.817LeuVal: 4.817 ± 0.072	0.564LeuTrp: 0.564 ± 0.022	3.91LeuTyr: 3.91 ± 0.062	0.0LeuXaa: 0.0 ± 0.0
Met	0.939MetAla: 0.939 ± 0.031	0.365MetCys: 0.365 ± 0.019	1.269MetAsp: 1.269 ± 0.034	1.267MetGlu: 1.267 ± 0.032	1.286MetPhe: 1.286 ± 0.035	0.856MetGly: 0.856 ± 0.029	0.364MetHis: 0.364 ± 0.019	2.075MetIle: 2.075 ± 0.047	2.501MetLys: 2.501 ± 0.048	1.718MetLeu: 1.718 ± 0.038	0.545MetMet: 0.545 ± 0.023	1.765MetAsn: 1.765 ± 0.042	0.718MetPro: 0.718 ± 0.025	0.546MetGln: 0.546 ± 0.022	0.908MetArg: 0.908 ± 0.03	1.603MetSer: 1.603 ± 0.034	1.083MetThr: 1.083 ± 0.032	1.167MetVal: 1.167 ± 0.036	0.139MetTrp: 0.139 ± 0.01	0.813MetTyr: 0.813 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	1.75AsnAla: 1.75 ± 0.045	1.127AsnCys: 1.127 ± 0.033	4.153AsnAsp: 4.153 ± 0.059	5.857AsnGlu: 5.857 ± 0.076	4.057AsnPhe: 4.057 ± 0.066	2.791AsnGly: 2.791 ± 0.062	1.243AsnHis: 1.243 ± 0.041	6.2AsnIle: 6.2 ± 0.069	7.067AsnLys: 7.067 ± 0.083	8.021AsnLeu: 8.021 ± 0.086	1.472AsnMet: 1.472 ± 0.04	5.556AsnAsn: 5.556 ± 0.095	2.477AsnPro: 2.477 ± 0.062	2.252AsnGln: 2.252 ± 0.052	2.404AsnArg: 2.404 ± 0.045	4.824AsnSer: 4.824 ± 0.074	3.408AsnThr: 3.408 ± 0.062	3.981AsnVal: 3.981 ± 0.066	0.384AsnTrp: 0.384 ± 0.017	3.018AsnTyr: 3.018 ± 0.055	0.001AsnXaa: 0.001 ± 0.001
Pro	0.914ProAla: 0.914 ± 0.036	0.433ProCys: 0.433 ± 0.022	1.401ProAsp: 1.401 ± 0.037	2.105ProGlu: 2.105 ± 0.049	2.078ProPhe: 2.078 ± 0.055	1.18ProGly: 1.18 ± 0.037	0.642ProHis: 0.642 ± 0.023	2.574ProIle: 2.574 ± 0.054	2.648ProLys: 2.648 ± 0.06	3.21ProLeu: 3.21 ± 0.064	0.574ProMet: 0.574 ± 0.022	2.058ProAsn: 2.058 ± 0.049	1.624ProPro: 1.624 ± 0.069	1.045ProGln: 1.045 ± 0.039	1.021ProArg: 1.021 ± 0.034	2.836ProSer: 2.836 ± 0.067	1.802ProThr: 1.802 ± 0.057	1.756ProVal: 1.756 ± 0.052	0.145ProTrp: 0.145 ± 0.013	1.222ProTyr: 1.222 ± 0.042	0.001ProXaa: 0.001 ± 0.001
Gln	0.951GlnAla: 0.951 ± 0.033	0.376GlnCys: 0.376 ± 0.019	1.578GlnAsp: 1.578 ± 0.04	2.154GlnGlu: 2.154 ± 0.047	1.281GlnPhe: 1.281 ± 0.036	1.14GlnGly: 1.14 ± 0.04	0.458GlnHis: 0.458 ± 0.016	2.505GlnIle: 2.505 ± 0.042	2.812GlnLys: 2.812 ± 0.052	2.086GlnLeu: 2.086 ± 0.046	0.657GlnMet: 0.657 ± 0.026	2.334GlnAsn: 2.334 ± 0.054	1.047GlnPro: 1.047 ± 0.045	0.862GlnGln: 0.862 ± 0.037	1.074GlnArg: 1.074 ± 0.033	1.851GlnSer: 1.851 ± 0.042	1.417GlnThr: 1.417 ± 0.042	1.196GlnVal: 1.196 ± 0.036	0.13GlnTrp: 0.13 ± 0.011	0.932GlnTyr: 0.932 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	1.221ArgAla: 1.221 ± 0.037	0.566ArgCys: 0.566 ± 0.023	2.161ArgAsp: 2.161 ± 0.057	2.877ArgGlu: 2.877 ± 0.056	1.928ArgPhe: 1.928 ± 0.044	1.467ArgGly: 1.467 ± 0.04	0.722ArgHis: 0.722 ± 0.029	3.348ArgIle: 3.348 ± 0.054	3.791ArgLys: 3.791 ± 0.06	3.421ArgLeu: 3.421 ± 0.059	0.969ArgMet: 0.969 ± 0.027	2.635ArgAsn: 2.635 ± 0.05	1.101ArgPro: 1.101 ± 0.034	0.999ArgGln: 0.999 ± 0.035	2.08ArgArg: 2.08 ± 0.056	2.535ArgSer: 2.535 ± 0.051	1.716ArgThr: 1.716 ± 0.043	1.885ArgVal: 1.885 ± 0.04	0.203ArgTrp: 0.203 ± 0.014	1.433ArgTyr: 1.433 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	1.996SerAla: 1.996 ± 0.046	1.206SerCys: 1.206 ± 0.031	3.932SerAsp: 3.932 ± 0.069	4.939SerGlu: 4.939 ± 0.074	4.597SerPhe: 4.597 ± 0.062	2.881SerGly: 2.881 ± 0.063	1.312SerHis: 1.312 ± 0.04	6.162SerIle: 6.162 ± 0.086	7.642SerLys: 7.642 ± 0.1	7.979SerLeu: 7.979 ± 0.099	1.396SerMet: 1.396 ± 0.034	5.138SerAsn: 5.138 ± 0.078	2.422SerPro: 2.422 ± 0.06	2.155SerGln: 2.155 ± 0.053	2.643SerArg: 2.643 ± 0.05	6.745SerSer: 6.745 ± 0.13	3.898SerThr: 3.898 ± 0.075	3.668SerVal: 3.668 ± 0.068	0.412SerTrp: 0.412 ± 0.019	3.037SerTyr: 3.037 ± 0.055	0.0SerXaa: 0.0 ± 0.0
Thr	1.618ThrAla: 1.618 ± 0.043	0.77ThrCys: 0.77 ± 0.027	2.355ThrAsp: 2.355 ± 0.054	3.225ThrGlu: 3.225 ± 0.059	2.795ThrPhe: 2.795 ± 0.053	2.101ThrGly: 2.101 ± 0.05	0.808ThrHis: 0.808 ± 0.026	3.951ThrIle: 3.951 ± 0.062	4.452ThrLys: 4.452 ± 0.075	4.629ThrLeu: 4.629 ± 0.069	0.884ThrMet: 0.884 ± 0.03	3.216ThrAsn: 3.216 ± 0.062	2.304ThrPro: 2.304 ± 0.062	1.457ThrGln: 1.457 ± 0.041	1.86ThrArg: 1.86 ± 0.042	3.81ThrSer: 3.81 ± 0.067	3.059ThrThr: 3.059 ± 0.069	2.708ThrVal: 2.708 ± 0.052	0.232ThrTrp: 0.232 ± 0.015	1.842ThrTyr: 1.842 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	1.739ValAla: 1.739 ± 0.044	1.054ValCys: 1.054 ± 0.029	3.455ValAsp: 3.455 ± 0.058	3.782ValGlu: 3.782 ± 0.071	3.418ValPhe: 3.418 ± 0.063	2.274ValGly: 2.274 ± 0.05	0.964ValHis: 0.964 ± 0.03	4.476ValIle: 4.476 ± 0.058	4.931ValLys: 4.931 ± 0.074	5.507ValLeu: 5.507 ± 0.074	1.103ValMet: 1.103 ± 0.032	3.375ValAsn: 3.375 ± 0.057	1.53ValPro: 1.53 ± 0.042	1.376ValGln: 1.376 ± 0.032	1.763ValArg: 1.763 ± 0.042	3.801ValSer: 3.801 ± 0.056	2.264ValThr: 2.264 ± 0.05	3.371ValVal: 3.371 ± 0.072	0.316ValTrp: 0.316 ± 0.018	2.582ValTyr: 2.582 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.265TrpAla: 0.265 ± 0.015	0.121TrpCys: 0.121 ± 0.011	0.365TrpAsp: 0.365 ± 0.018	0.374TrpGlu: 0.374 ± 0.019	0.327TrpPhe: 0.327 ± 0.017	0.256TrpGly: 0.256 ± 0.018	0.097TrpHis: 0.097 ± 0.009	0.474TrpIle: 0.474 ± 0.022	0.511TrpLys: 0.511 ± 0.019	0.543TrpLeu: 0.543 ± 0.025	0.139TrpMet: 0.139 ± 0.01	0.375TrpAsn: 0.375 ± 0.018	0.135TrpPro: 0.135 ± 0.012	0.115TrpGln: 0.115 ± 0.011	0.281TrpArg: 0.281 ± 0.015	0.45TrpSer: 0.45 ± 0.019	0.279TrpThr: 0.279 ± 0.014	0.315TrpVal: 0.315 ± 0.019	0.047TrpTrp: 0.047 ± 0.007	0.266TrpTyr: 0.266 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.399TyrAla: 1.399 ± 0.038	0.739TyrCys: 0.739 ± 0.025	2.566TyrAsp: 2.566 ± 0.052	3.242TyrGlu: 3.242 ± 0.054	2.937TyrPhe: 2.937 ± 0.07	1.742TyrGly: 1.742 ± 0.045	0.813TyrHis: 0.813 ± 0.03	3.016TyrIle: 3.016 ± 0.052	4.337TyrLys: 4.337 ± 0.081	4.663TyrLeu: 4.663 ± 0.076	0.756TyrMet: 0.756 ± 0.023	2.763TyrAsn: 2.763 ± 0.057	1.305TyrPro: 1.305 ± 0.048	1.08TyrGln: 1.08 ± 0.036	1.379TyrArg: 1.379 ± 0.038	3.194TyrSer: 3.194 ± 0.057	1.844TyrThr: 1.844 ± 0.048	2.196TyrVal: 2.196 ± 0.054	0.269TyrTrp: 0.269 ± 0.015	1.803TyrTyr: 1.803 ± 0.045	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.0XaaLeu: 0.0 ± 0.0	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4398 proteins (1099605 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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