Paenibacillus swuensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4095 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A172TK27|A0A172TK27_9BACL Purine nucleoside phosphorylase OS=Paenibacillus swuensis OX=1178515 GN=SY83_15115 PE=3 SV=1
MM1 pKa = 7.5SEE3 pKa = 4.2HH4 pKa = 6.28VHH6 pKa = 7.37DD7 pKa = 4.88EE8 pKa = 4.39NCNHH12 pKa = 6.47DD13 pKa = 4.53HH14 pKa = 7.21EE15 pKa = 5.02DD16 pKa = 3.36QVFIITDD23 pKa = 3.29ADD25 pKa = 3.96GNEE28 pKa = 4.32HH29 pKa = 6.17EE30 pKa = 4.79MVMVYY35 pKa = 9.03TFEE38 pKa = 5.34SEE40 pKa = 4.16EE41 pKa = 3.67QVYY44 pKa = 10.34AVLLDD49 pKa = 3.73KK50 pKa = 11.32NNPEE54 pKa = 3.69EE55 pKa = 4.73DD56 pKa = 3.56GVIFRR61 pKa = 11.84IEE63 pKa = 4.12EE64 pKa = 3.9EE65 pKa = 4.3GEE67 pKa = 4.02EE68 pKa = 4.14AFLEE72 pKa = 4.61SIEE75 pKa = 6.48DD76 pKa = 3.37EE77 pKa = 4.77DD78 pKa = 3.27EE79 pKa = 4.08WNRR82 pKa = 11.84VVAVYY87 pKa = 10.29NQIAAEE93 pKa = 3.96EE94 pKa = 4.47SEE96 pKa = 4.44NVV98 pKa = 3.24

Molecular weight: 11.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A172TGL9|A0A172TGL9_9BACL ABC transporter substrate-binding protein OS=Paenibacillus swuensis OX=1178515 GN=SY83_07280 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.84PNVSKK11 pKa = 10.73RR12 pKa = 11.84AKK14 pKa = 8.72VHH16 pKa = 5.43GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.78VLAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.05GRR39 pKa = 11.84KK40 pKa = 8.85VISAA44 pKa = 4.05

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4095

0

4095

1341642

26

2618

327.6

36.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.197 ± 0.045

0.718 ± 0.012

5.046 ± 0.024

6.675 ± 0.042

4.13 ± 0.027

7.509 ± 0.036

2.194 ± 0.016

6.558 ± 0.037

5.284 ± 0.031

10.0 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.935 ± 0.021

3.992 ± 0.032

4.056 ± 0.022

3.815 ± 0.022

4.949 ± 0.027

6.111 ± 0.036

5.698 ± 0.038

7.248 ± 0.028

1.298 ± 0.018

3.586 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski