Pseudomonas sp. GW456-E7

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7747 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N8FK52|A0A2N8FK52_9PSED Spermidine/putrescine ABC transporter ATP-binding protein (Fragment) OS=Pseudomonas sp. GW456-E7 OX=2070669 GN=C1X64_37850 PE=4 SV=1
LL1 pKa = 8.1DD2 pKa = 3.83MAQLLLDD9 pKa = 3.67VLCFGKK15 pKa = 10.42DD16 pKa = 2.86ADD18 pKa = 4.92LPDD21 pKa = 4.22GLLDD25 pKa = 3.53QEE27 pKa = 4.63PYY29 pKa = 10.84YY30 pKa = 11.17VADD33 pKa = 3.42CHH35 pKa = 7.6LYY37 pKa = 9.13PQHH40 pKa = 6.74FKK42 pKa = 11.29QNGQIPEE49 pKa = 4.11TAEE52 pKa = 3.99DD53 pKa = 3.97LVIEE57 pKa = 5.56AIDD60 pKa = 4.89LPDD63 pKa = 4.24GLLKK67 pKa = 10.74GDD69 pKa = 3.78TEE71 pKa = 4.37IVSFSAAAPGTSVDD85 pKa = 3.36

Molecular weight: 9.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N8GG18|A0A2N8GG18_9PSED Glycine--tRNA ligase beta subunit OS=Pseudomonas sp. GW456-E7 OX=2070669 GN=glyS PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.38GRR39 pKa = 11.84ARR41 pKa = 11.84LAVV44 pKa = 3.42

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7747

0

7747

2097422

20

4332

270.7

29.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.553 ± 0.034

0.986 ± 0.011

5.393 ± 0.022

5.748 ± 0.027

3.79 ± 0.017

7.951 ± 0.035

2.26 ± 0.014

5.106 ± 0.022

3.84 ± 0.026

11.359 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.014

3.239 ± 0.021

4.785 ± 0.022

4.374 ± 0.025

5.98 ± 0.031

5.935 ± 0.022

5.097 ± 0.028

7.174 ± 0.024

1.394 ± 0.012

2.602 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski