Amino acid dipepetide frequency for Pseudomonas sp. GW456-E7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.833AlaAla: 11.833 ± 0.106	1.168AlaCys: 1.168 ± 0.026	5.77AlaAsp: 5.77 ± 0.063	6.549AlaGlu: 6.549 ± 0.064	3.836AlaPhe: 3.836 ± 0.044	8.596AlaGly: 8.596 ± 0.068	2.05AlaHis: 2.05 ± 0.031	5.296AlaIle: 5.296 ± 0.059	3.975AlaLys: 3.975 ± 0.058	12.591AlaLeu: 12.591 ± 0.094	2.959AlaMet: 2.959 ± 0.039	3.117AlaAsn: 3.117 ± 0.05	4.505AlaPro: 4.505 ± 0.063	4.817AlaGln: 4.817 ± 0.053	6.325AlaArg: 6.325 ± 0.066	5.945AlaSer: 5.945 ± 0.052	4.831AlaThr: 4.831 ± 0.049	7.368AlaVal: 7.368 ± 0.065	1.452AlaTrp: 1.452 ± 0.031	2.55AlaTyr: 2.55 ± 0.034	0.0AlaXaa: 0.0 ± 0.0
Cys	1.074CysAla: 1.074 ± 0.025	0.139CysCys: 0.139 ± 0.009	0.519CysAsp: 0.519 ± 0.016	0.548CysGlu: 0.548 ± 0.017	0.356CysPhe: 0.356 ± 0.012	0.933CysGly: 0.933 ± 0.021	0.275CysHis: 0.275 ± 0.013	0.457CysIle: 0.457 ± 0.014	0.308CysLys: 0.308 ± 0.012	1.103CysLeu: 1.103 ± 0.025	0.222CysMet: 0.222 ± 0.011	0.289CysAsn: 0.289 ± 0.013	0.478CysPro: 0.478 ± 0.016	0.359CysGln: 0.359 ± 0.014	0.591CysArg: 0.591 ± 0.018	0.628CysSer: 0.628 ± 0.017	0.475CysThr: 0.475 ± 0.015	0.725CysVal: 0.725 ± 0.019	0.143CysTrp: 0.143 ± 0.009	0.24CysTyr: 0.24 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	5.437AspAla: 5.437 ± 0.063	0.529AspCys: 0.529 ± 0.017	2.901AspAsp: 2.901 ± 0.047	3.395AspGlu: 3.395 ± 0.043	2.187AspPhe: 2.187 ± 0.034	4.482AspGly: 4.482 ± 0.058	1.221AspHis: 1.221 ± 0.026	2.861AspIle: 2.861 ± 0.042	2.184AspLys: 2.184 ± 0.04	5.798AspLeu: 5.798 ± 0.06	1.276AspMet: 1.276 ± 0.023	1.733AspAsn: 1.733 ± 0.032	2.804AspPro: 2.804 ± 0.036	2.179AspGln: 2.179 ± 0.034	3.004AspArg: 3.004 ± 0.039	2.97AspSer: 2.97 ± 0.041	2.612AspThr: 2.612 ± 0.043	3.685AspVal: 3.685 ± 0.046	0.934AspTrp: 0.934 ± 0.023	1.74AspTyr: 1.74 ± 0.032	0.0AspXaa: 0.0 ± 0.0
Glu	6.127GluAla: 6.127 ± 0.066	0.436GluCys: 0.436 ± 0.013	2.647GluAsp: 2.647 ± 0.036	3.238GluGlu: 3.238 ± 0.049	1.936GluPhe: 1.936 ± 0.035	4.039GluGly: 4.039 ± 0.05	1.644GluHis: 1.644 ± 0.027	2.899GluIle: 2.899 ± 0.041	2.541GluLys: 2.541 ± 0.041	6.81GluLeu: 6.81 ± 0.063	1.43GluMet: 1.43 ± 0.03	1.726GluAsn: 1.726 ± 0.028	2.448GluPro: 2.448 ± 0.037	3.498GluGln: 3.498 ± 0.048	4.386GluArg: 4.386 ± 0.053	2.874GluSer: 2.874 ± 0.039	2.71GluThr: 2.71 ± 0.038	4.073GluVal: 4.073 ± 0.044	0.729GluTrp: 0.729 ± 0.016	1.286GluTyr: 1.286 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	4.046PheAla: 4.046 ± 0.046	0.47PheCys: 0.47 ± 0.015	2.499PheAsp: 2.499 ± 0.036	2.132PheGlu: 2.132 ± 0.035	1.603PhePhe: 1.603 ± 0.034	3.392PheGly: 3.392 ± 0.043	0.78PheHis: 0.78 ± 0.021	2.067PheIle: 2.067 ± 0.033	1.448PheLys: 1.448 ± 0.026	3.415PheLeu: 3.415 ± 0.04	0.881PheMet: 0.881 ± 0.023	1.49PheAsn: 1.49 ± 0.027	1.543PhePro: 1.543 ± 0.034	1.251PheGln: 1.251 ± 0.025	1.744PheArg: 1.744 ± 0.028	2.707PheSer: 2.707 ± 0.043	2.094PheThr: 2.094 ± 0.038	2.627PheVal: 2.627 ± 0.035	0.59PheTrp: 0.59 ± 0.018	1.121PheTyr: 1.121 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	7.244GlyAla: 7.244 ± 0.073	0.945GlyCys: 0.945 ± 0.024	3.931GlyAsp: 3.931 ± 0.052	4.625GlyGlu: 4.625 ± 0.05	3.471GlyPhe: 3.471 ± 0.039	6.051GlyGly: 6.051 ± 0.072	1.874GlyHis: 1.874 ± 0.029	4.417GlyIle: 4.417 ± 0.054	3.664GlyLys: 3.664 ± 0.046	8.947GlyLeu: 8.947 ± 0.078	2.267GlyMet: 2.267 ± 0.032	2.658GlyAsn: 2.658 ± 0.057	2.651GlyPro: 2.651 ± 0.039	3.552GlyGln: 3.552 ± 0.048	4.453GlyArg: 4.453 ± 0.048	4.798GlySer: 4.798 ± 0.057	4.047GlyThr: 4.047 ± 0.06	6.108GlyVal: 6.108 ± 0.051	1.319GlyTrp: 1.319 ± 0.027	2.484GlyTyr: 2.484 ± 0.039	0.0GlyXaa: 0.0 ± 0.0
His	2.141HisAla: 2.141 ± 0.032	0.327HisCys: 0.327 ± 0.012	1.192HisAsp: 1.192 ± 0.025	1.24HisGlu: 1.24 ± 0.025	1.006HisPhe: 1.006 ± 0.022	1.921HisGly: 1.921 ± 0.033	0.618HisHis: 0.618 ± 0.021	1.061HisIle: 1.061 ± 0.022	0.72HisLys: 0.72 ± 0.02	2.525HisLeu: 2.525 ± 0.038	0.569HisMet: 0.569 ± 0.016	0.68HisAsn: 0.68 ± 0.019	1.418HisPro: 1.418 ± 0.026	0.945HisGln: 0.945 ± 0.022	1.265HisArg: 1.265 ± 0.025	1.337HisSer: 1.337 ± 0.026	1.029HisThr: 1.029 ± 0.023	1.42HisVal: 1.42 ± 0.026	0.446HisTrp: 0.446 ± 0.016	0.742HisTyr: 0.742 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	6.174IleAla: 6.174 ± 0.059	0.558IleCys: 0.558 ± 0.018	3.453IleAsp: 3.453 ± 0.044	3.559IleGlu: 3.559 ± 0.039	1.656IlePhe: 1.656 ± 0.031	4.778IleGly: 4.778 ± 0.059	1.063IleHis: 1.063 ± 0.024	2.374IleIle: 2.374 ± 0.036	2.003IleLys: 2.003 ± 0.037	4.299IleLeu: 4.299 ± 0.046	0.932IleMet: 0.932 ± 0.024	1.918IleAsn: 1.918 ± 0.031	2.392IlePro: 2.392 ± 0.034	1.696IleGln: 1.696 ± 0.03	2.815IleArg: 2.815 ± 0.038	3.258IleSer: 3.258 ± 0.042	2.81IleThr: 2.81 ± 0.036	3.503IleVal: 3.503 ± 0.049	0.547IleTrp: 0.547 ± 0.016	1.27IleTyr: 1.27 ± 0.025	0.0IleXaa: 0.0 ± 0.0
Lys	4.352LysAla: 4.352 ± 0.054	0.222LysCys: 0.222 ± 0.011	2.08LysAsp: 2.08 ± 0.039	2.129LysGlu: 2.129 ± 0.036	0.967LysPhe: 0.967 ± 0.021	2.884LysGly: 2.884 ± 0.041	0.889LysHis: 0.889 ± 0.02	1.902LysIle: 1.902 ± 0.034	1.724LysLys: 1.724 ± 0.041	4.074LysLeu: 4.074 ± 0.052	0.875LysMet: 0.875 ± 0.022	1.289LysAsn: 1.289 ± 0.027	2.232LysPro: 2.232 ± 0.045	1.722LysGln: 1.722 ± 0.032	2.427LysArg: 2.427 ± 0.033	2.09LysSer: 2.09 ± 0.034	2.226LysThr: 2.226 ± 0.04	3.036LysVal: 3.036 ± 0.045	0.446LysTrp: 0.446 ± 0.013	0.837LysTyr: 0.837 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	12.736LeuAla: 12.736 ± 0.103	1.165LeuCys: 1.165 ± 0.026	6.392LeuAsp: 6.392 ± 0.058	6.258LeuGlu: 6.258 ± 0.057	4.203LeuPhe: 4.203 ± 0.053	8.787LeuGly: 8.787 ± 0.073	2.402LeuHis: 2.402 ± 0.037	5.799LeuIle: 5.799 ± 0.065	4.811LeuLys: 4.811 ± 0.057	12.732LeuLeu: 12.732 ± 0.121	2.684LeuMet: 2.684 ± 0.037	3.867LeuAsn: 3.867 ± 0.048	6.005LeuPro: 6.005 ± 0.067	4.454LeuGln: 4.454 ± 0.052	6.658LeuArg: 6.658 ± 0.067	7.326LeuSer: 7.326 ± 0.064	6.02LeuThr: 6.02 ± 0.061	7.42LeuVal: 7.42 ± 0.073	1.318LeuTrp: 1.318 ± 0.03	2.556LeuTyr: 2.556 ± 0.043	0.0LeuXaa: 0.0 ± 0.0
Met	2.714MetAla: 2.714 ± 0.039	0.187MetCys: 0.187 ± 0.01	1.11MetAsp: 1.11 ± 0.023	1.046MetGlu: 1.046 ± 0.024	0.8MetPhe: 0.8 ± 0.022	1.76MetGly: 1.76 ± 0.033	0.523MetHis: 0.523 ± 0.017	1.321MetIle: 1.321 ± 0.025	1.061MetLys: 1.061 ± 0.023	2.794MetLeu: 2.794 ± 0.039	0.597MetMet: 0.597 ± 0.023	0.969MetAsn: 0.969 ± 0.023	1.348MetPro: 1.348 ± 0.024	1.034MetGln: 1.034 ± 0.023	1.438MetArg: 1.438 ± 0.026	1.816MetSer: 1.816 ± 0.031	1.546MetThr: 1.546 ± 0.027	1.651MetVal: 1.651 ± 0.029	0.195MetTrp: 0.195 ± 0.011	0.423MetTyr: 0.423 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.347AsnAla: 3.347 ± 0.046	0.294AsnCys: 0.294 ± 0.012	1.827AsnAsp: 1.827 ± 0.039	1.675AsnGlu: 1.675 ± 0.031	1.164AsnPhe: 1.164 ± 0.027	2.9AsnGly: 2.9 ± 0.047	0.681AsnHis: 0.681 ± 0.018	1.677AsnIle: 1.677 ± 0.03	1.096AsnLys: 1.096 ± 0.026	3.549AsnLeu: 3.549 ± 0.045	0.67AsnMet: 0.67 ± 0.016	1.117AsnAsn: 1.117 ± 0.031	2.09AsnPro: 2.09 ± 0.035	1.343AsnGln: 1.343 ± 0.031	1.915AsnArg: 1.915 ± 0.029	1.783AsnSer: 1.783 ± 0.033	1.672AsnThr: 1.672 ± 0.028	2.234AsnVal: 2.234 ± 0.038	0.496AsnTrp: 0.496 ± 0.016	0.863AsnTyr: 0.863 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	5.235ProAla: 5.235 ± 0.067	0.359ProCys: 0.359 ± 0.014	2.926ProAsp: 2.926 ± 0.036	3.22ProGlu: 3.22 ± 0.043	1.849ProPhe: 1.849 ± 0.029	3.931ProGly: 3.931 ± 0.049	1.035ProHis: 1.035 ± 0.023	2.135ProIle: 2.135 ± 0.034	1.729ProLys: 1.729 ± 0.031	5.462ProLeu: 5.462 ± 0.064	1.165ProMet: 1.165 ± 0.024	1.481ProAsn: 1.481 ± 0.03	1.893ProPro: 1.893 ± 0.039	2.263ProGln: 2.263 ± 0.032	2.303ProArg: 2.303 ± 0.036	2.624ProSer: 2.624 ± 0.033	2.373ProThr: 2.373 ± 0.033	3.978ProVal: 3.978 ± 0.054	0.684ProTrp: 0.684 ± 0.019	1.208ProTyr: 1.208 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	5.283GlnAla: 5.283 ± 0.063	0.324GlnCys: 0.324 ± 0.014	1.817GlnAsp: 1.817 ± 0.029	1.875GlnGlu: 1.875 ± 0.032	1.403GlnPhe: 1.403 ± 0.025	3.156GlnGly: 3.156 ± 0.043	1.098GlnHis: 1.098 ± 0.024	2.149GlnIle: 2.149 ± 0.039	1.498GlnLys: 1.498 ± 0.029	5.181GlnLeu: 5.181 ± 0.056	1.091GlnMet: 1.091 ± 0.024	1.219GlnAsn: 1.219 ± 0.023	2.274GlnPro: 2.274 ± 0.034	2.472GlnGln: 2.472 ± 0.044	3.246GlnArg: 3.246 ± 0.042	2.427GlnSer: 2.427 ± 0.037	2.12GlnThr: 2.12 ± 0.036	3.366GlnVal: 3.366 ± 0.044	0.723GlnTrp: 0.723 ± 0.02	1.015GlnTyr: 1.015 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	5.367ArgAla: 5.367 ± 0.057	0.561ArgCys: 0.561 ± 0.018	3.212ArgAsp: 3.212 ± 0.045	3.913ArgGlu: 3.913 ± 0.048	2.604ArgPhe: 2.604 ± 0.037	3.74ArgGly: 3.74 ± 0.043	1.591ArgHis: 1.591 ± 0.031	3.271ArgIle: 3.271 ± 0.042	2.164ArgLys: 2.164 ± 0.037	7.504ArgLeu: 7.504 ± 0.067	1.56ArgMet: 1.56 ± 0.028	1.912ArgAsn: 1.912 ± 0.038	2.537ArgPro: 2.537 ± 0.036	3.035ArgGln: 3.035 ± 0.043	3.767ArgArg: 3.767 ± 0.044	3.274ArgSer: 3.274 ± 0.044	2.715ArgThr: 2.715 ± 0.037	4.216ArgVal: 4.216 ± 0.048	0.952ArgTrp: 0.952 ± 0.023	1.901ArgTyr: 1.901 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	6.078SerAla: 6.078 ± 0.052	0.485SerCys: 0.485 ± 0.016	2.991SerAsp: 2.991 ± 0.038	3.18SerGlu: 3.18 ± 0.041	2.401SerPhe: 2.401 ± 0.034	5.379SerGly: 5.379 ± 0.056	1.354SerHis: 1.354 ± 0.028	3.076SerIle: 3.076 ± 0.039	2.063SerLys: 2.063 ± 0.034	6.959SerLeu: 6.959 ± 0.066	1.462SerMet: 1.462 ± 0.026	1.876SerAsn: 1.876 ± 0.033	2.768SerPro: 2.768 ± 0.04	2.352SerGln: 2.352 ± 0.036	3.557SerArg: 3.557 ± 0.042	3.526SerSer: 3.526 ± 0.046	3.069SerThr: 3.069 ± 0.04	4.348SerVal: 4.348 ± 0.051	0.793SerTrp: 0.793 ± 0.02	1.633SerTyr: 1.633 ± 0.028	0.0SerXaa: 0.0 ± 0.0
Thr	5.366ThrAla: 5.366 ± 0.057	0.494ThrCys: 0.494 ± 0.017	2.647ThrAsp: 2.647 ± 0.039	2.68ThrGlu: 2.68 ± 0.037	1.998ThrPhe: 1.998 ± 0.034	4.463ThrGly: 4.463 ± 0.064	1.12ThrHis: 1.12 ± 0.022	2.3ThrIle: 2.3 ± 0.04	1.33ThrLys: 1.33 ± 0.024	6.682ThrLeu: 6.682 ± 0.067	0.953ThrMet: 0.953 ± 0.023	1.333ThrAsn: 1.333 ± 0.026	3.163ThrPro: 3.163 ± 0.044	2.016ThrGln: 2.016 ± 0.034	2.953ThrArg: 2.953 ± 0.038	2.878ThrSer: 2.878 ± 0.039	2.649ThrThr: 2.649 ± 0.047	3.878ThrVal: 3.878 ± 0.049	0.7ThrTrp: 0.7 ± 0.017	1.37ThrTyr: 1.37 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	7.308ValAla: 7.308 ± 0.067	0.743ValCys: 0.743 ± 0.021	3.975ValAsp: 3.975 ± 0.049	4.326ValGlu: 4.326 ± 0.051	2.782ValPhe: 2.782 ± 0.039	5.307ValGly: 5.307 ± 0.06	1.429ValHis: 1.429 ± 0.026	4.164ValIle: 4.164 ± 0.051	2.678ValLys: 2.678 ± 0.04	8.059ValLeu: 8.059 ± 0.077	1.834ValMet: 1.834 ± 0.029	2.408ValAsn: 2.408 ± 0.034	3.374ValPro: 3.374 ± 0.043	2.679ValGln: 2.679 ± 0.038	4.077ValArg: 4.077 ± 0.045	4.598ValSer: 4.598 ± 0.045	3.973ValThr: 3.973 ± 0.059	5.513ValVal: 5.513 ± 0.074	0.853ValTrp: 0.853 ± 0.021	1.661ValTyr: 1.661 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	1.147TrpAla: 1.147 ± 0.026	0.163TrpCys: 0.163 ± 0.009	0.628TrpAsp: 0.628 ± 0.018	0.589TrpGlu: 0.589 ± 0.015	0.558TrpPhe: 0.558 ± 0.018	0.89TrpGly: 0.89 ± 0.025	0.349TrpHis: 0.349 ± 0.015	0.701TrpIle: 0.701 ± 0.018	0.51TrpLys: 0.51 ± 0.017	2.069TrpLeu: 2.069 ± 0.038	0.385TrpMet: 0.385 ± 0.014	0.505TrpAsn: 0.505 ± 0.016	0.63TrpPro: 0.63 ± 0.017	0.846TrpGln: 0.846 ± 0.02	0.985TrpArg: 0.985 ± 0.024	0.876TrpSer: 0.876 ± 0.021	0.627TrpThr: 0.627 ± 0.016	0.915TrpVal: 0.915 ± 0.02	0.211TrpTrp: 0.211 ± 0.01	0.357TrpTyr: 0.357 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.526TyrAla: 2.526 ± 0.037	0.293TyrCys: 0.293 ± 0.012	1.415TyrAsp: 1.415 ± 0.032	1.3TyrGlu: 1.3 ± 0.031	1.121TyrPhe: 1.121 ± 0.024	2.119TyrGly: 2.119 ± 0.033	0.609TyrHis: 0.609 ± 0.017	1.129TyrIle: 1.129 ± 0.025	0.895TyrLys: 0.895 ± 0.023	3.038TyrLeu: 3.038 ± 0.04	0.524TyrMet: 0.524 ± 0.018	0.807TyrAsn: 0.807 ± 0.023	1.3TyrPro: 1.3 ± 0.023	1.22TyrGln: 1.22 ± 0.019	1.888TyrArg: 1.888 ± 0.031	1.616TyrSer: 1.616 ± 0.031	1.375TyrThr: 1.375 ± 0.031	1.677TyrVal: 1.677 ± 0.034	0.406TyrTrp: 0.406 ± 0.013	0.761TyrTyr: 0.761 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7747 proteins (2097422 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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